Clostridium ventriculi (Sarcina ventriculi)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firm

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A174A8K5|A0A174A8K5_CLOVE Hypoxanthine phosphoribosyltransferase OS=Clostridium ventriculi OX=1267 GN=hpt_1 PE=3 SV=1
MM1 pKa = 7.53ACDD4 pKa = 3.36NTIQVLNGSVLTFEE18 pKa = 5.64SEE20 pKa = 4.27VQICNSITNLTKK32 pKa = 10.76LQFRR36 pKa = 11.84DD37 pKa = 3.29ILQGGLKK44 pKa = 8.78VTSDD48 pKa = 3.54QVNLTINNTSSTATAIVSEE67 pKa = 4.58TNNPEE72 pKa = 3.62DD73 pKa = 4.05TITVEE78 pKa = 4.1ILAANLGNDD87 pKa = 3.37YY88 pKa = 10.8PVTVKK93 pKa = 10.71VKK95 pKa = 10.06FQATVNDD102 pKa = 4.07LDD104 pKa = 4.57ALLEE108 pKa = 4.38NADD111 pKa = 4.37DD112 pKa = 3.82PTNAGKK118 pKa = 8.48LTNTGTFTALDD129 pKa = 3.63NAGASIGKK137 pKa = 8.8PATVTLADD145 pKa = 4.02TNVEE149 pKa = 4.22EE150 pKa = 4.49YY151 pKa = 10.59SPEE154 pKa = 3.71VSGYY158 pKa = 10.48SITVCPSEE166 pKa = 4.23NDD168 pKa = 3.31AKK170 pKa = 11.1FEE172 pKa = 4.94AKK174 pKa = 10.35LCLNTLCGDD183 pKa = 3.86YY184 pKa = 11.0TDD186 pKa = 4.11GKK188 pKa = 10.87YY189 pKa = 10.89VLDD192 pKa = 3.62VTAPDD197 pKa = 5.05GISFYY202 pKa = 11.21EE203 pKa = 4.75DD204 pKa = 3.01ATSTPPAAVEE214 pKa = 4.64SYY216 pKa = 9.76TGCCCKK222 pKa = 9.6STKK225 pKa = 9.74IEE227 pKa = 4.08MATPPVISADD237 pKa = 3.38NKK239 pKa = 9.65TVTITIASNEE249 pKa = 3.74VDD251 pKa = 3.4VALEE255 pKa = 3.87NDD257 pKa = 3.9TDD259 pKa = 4.24DD260 pKa = 3.86SCNNNISVYY269 pKa = 10.04IPCRR273 pKa = 11.84FDD275 pKa = 3.31NAASACTNEE284 pKa = 3.81PLIINGSIKK293 pKa = 10.07FTDD296 pKa = 3.72DD297 pKa = 3.08TDD299 pKa = 3.52NTLAEE304 pKa = 4.45LKK306 pKa = 11.03NFGLYY311 pKa = 10.92VNVDD315 pKa = 3.68CGNLVGVSKK324 pKa = 9.83TILCC328 pKa = 4.43

Molecular weight: 34.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A174BA25|A0A174BA25_CLOVE Oxygen-independent coproporphyrinogen-III oxidase 2 OS=Clostridium ventriculi OX=1267 GN=hemZ_2 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84EE4 pKa = 3.27RR5 pKa = 11.84DD6 pKa = 3.31ARR8 pKa = 11.84KK9 pKa = 9.28GGKK12 pKa = 7.47MRR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.94KK19 pKa = 9.33VCIFCVDD26 pKa = 3.15KK27 pKa = 11.51AEE29 pKa = 4.63SIDD32 pKa = 3.83YY33 pKa = 10.92KK34 pKa = 10.98DD35 pKa = 3.54VPKK38 pKa = 10.47LKK40 pKa = 10.42KK41 pKa = 10.69YY42 pKa = 7.85ITEE45 pKa = 4.07RR46 pKa = 11.84GKK48 pKa = 10.04ILPRR52 pKa = 11.84RR53 pKa = 11.84ISGTCAKK60 pKa = 9.97HH61 pKa = 5.4QRR63 pKa = 11.84QLTEE67 pKa = 4.3AIKK70 pKa = 10.27RR71 pKa = 11.84SRR73 pKa = 11.84NIALLPFTTEE83 pKa = 3.59

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2228

0

2228

702555

30

2730

315.3

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.361 ± 0.056

1.272 ± 0.021

5.512 ± 0.039

7.187 ± 0.069

4.589 ± 0.042

6.302 ± 0.051

1.289 ± 0.02

10.316 ± 0.064

9.311 ± 0.057

9.178 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.026

6.961 ± 0.067

2.621 ± 0.028

2.15 ± 0.025

3.207 ± 0.037

6.262 ± 0.047

4.812 ± 0.045

6.414 ± 0.045

0.622 ± 0.016

4.113 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski