Torque teno mini virus 6
Average proteome isoelectric point is 7.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9QU37|Q9QU37_9VIRU Uncharacterized protein OS=Torque teno mini virus 6 OX=687374 PE=4 SV=1
MM1 pKa = 7.06 NFEE4 pKa = 4.28 YY5 pKa = 10.1 PDD7 pKa = 3.79 YY8 pKa = 10.96 SPKK11 pKa = 10.34 EE12 pKa = 3.99 LQLQWINNIVHH23 pKa = 5.72 THH25 pKa = 7.16 DD26 pKa = 5.23 LLCKK30 pKa = 8.35 CTKK33 pKa = 9.86 PLEE36 pKa = 4.26 HH37 pKa = 7.01 TIHH40 pKa = 7.04 GIIKK44 pKa = 9.73 QEE46 pKa = 3.87 PNLRR50 pKa = 11.84 LEE52 pKa = 4.55 KK53 pKa = 9.4 EE54 pKa = 4.49 TKK56 pKa = 10.12 KK57 pKa = 10.92 LLEE60 pKa = 4.48 KK61 pKa = 10.69 CLTSTEE67 pKa = 4.34 DD68 pKa = 3.32 GSLVEE73 pKa = 5.22 DD74 pKa = 4.41 ALDD77 pKa = 3.88 GLGDD81 pKa = 3.99 GEE83 pKa = 4.79 LEE85 pKa = 4.26 ALFAEE90 pKa = 4.65 NTGEE94 pKa = 4.08 DD95 pKa = 3.34 TGG97 pKa = 4.11
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.576
IPC2_protein 4.609
IPC_protein 4.495
Toseland 4.368
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.291
Rodwell 4.342
Grimsley 4.279
Solomon 4.431
Lehninger 4.393
Nozaki 4.546
DTASelect 4.66
Thurlkill 4.355
EMBOSS 4.317
Sillero 4.609
Patrickios 3.694
IPC_peptide 4.444
IPC2_peptide 4.609
IPC2.peptide.svr19 4.525
Protein with the highest isoelectric point:
>tr|Q9QU36|Q9QU36_9VIRU Capsid protein OS=Torque teno mini virus 6 OX=687374 PE=3 SV=1
MM1 pKa = 7.51 INLVKK6 pKa = 10.56 HH7 pKa = 5.99 ILNMTFVLRR16 pKa = 11.84 LEE18 pKa = 4.57 DD19 pKa = 5.83 VPLQWKK25 pKa = 10.47 NSVTLQNKK33 pKa = 7.67 TNIPYY38 pKa = 9.79 PILSSKK44 pKa = 7.28 QLRR47 pKa = 11.84 YY48 pKa = 9.92 RR49 pKa = 11.84 FQHH52 pKa = 5.81 QPLKK56 pKa = 10.49 PISTTLTKK64 pKa = 10.72 EE65 pKa = 3.71 KK66 pKa = 10.91 DD67 pKa = 3.56 SLQKK71 pKa = 10.94 KK72 pKa = 9.45 LLNEE76 pKa = 4.3 SKK78 pKa = 9.37 KK79 pKa = 9.36 TMEE82 pKa = 4.35 LNKK85 pKa = 10.11 LFCRR89 pKa = 11.84 LQEE92 pKa = 4.06 QQWTSRR98 pKa = 11.84 HH99 pKa = 4.46 QSKK102 pKa = 9.65 AHH104 pKa = 6.08 HH105 pKa = 5.71 RR106 pKa = 11.84 TKK108 pKa = 10.46 HH109 pKa = 4.54 RR110 pKa = 11.84 QRR112 pKa = 11.84 KK113 pKa = 7.72 KK114 pKa = 9.86 KK115 pKa = 9.65 RR116 pKa = 11.84 RR117 pKa = 11.84 HH118 pKa = 5.75 KK119 pKa = 10.61 SSSSSDD125 pKa = 3.41 SEE127 pKa = 4.43 GNNDD131 pKa = 3.29 SSSSEE136 pKa = 4.04 SSSYY140 pKa = 11.27
Molecular weight: 16.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.195
IPC2_protein 9.648
IPC_protein 9.736
Toseland 10.716
ProMoST 10.218
Dawson 10.789
Bjellqvist 10.379
Wikipedia 10.906
Rodwell 11.389
Grimsley 10.818
Solomon 10.833
Lehninger 10.818
Nozaki 10.687
DTASelect 10.379
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.701
Patrickios 11.111
IPC_peptide 10.847
IPC2_peptide 8.843
IPC2.peptide.svr19 8.705
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
893
97
656
297.7
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.576 ± 0.572
1.792 ± 0.349
4.479 ± 0.642
5.151 ± 2.129
3.919 ± 0.944
4.591 ± 1.127
2.576 ± 1.082
6.271 ± 1.089
8.511 ± 1.433
9.183 ± 2.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.792 ± 0.172
6.159 ± 0.23
6.271 ± 1.29
6.159 ± 0.709
5.711 ± 1.061
6.607 ± 2.494
8.511 ± 0.74
2.912 ± 0.232
2.24 ± 0.512
4.591 ± 1.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here