Marinococcus luteus
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2897 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H2WA80|A0A1H2WA80_9BACI Two-component signal transduction system YycFG regulatory protein YycI OS=Marinococcus luteus OX=1122204 GN=SAMN05421781_2336 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 9.11 EE3 pKa = 4.28 TIKK6 pKa = 10.61 SWTLSILVIGSLLLTWQNWSFQPEE30 pKa = 4.32 YY31 pKa = 10.84 EE32 pKa = 4.17 NVNSSEE38 pKa = 4.34 SVQEE42 pKa = 3.9 TTEE45 pKa = 3.78 IAEE48 pKa = 4.03 TRR50 pKa = 11.84 EE51 pKa = 3.94 AGEE54 pKa = 4.29 VVRR57 pKa = 11.84 PTQGVVRR64 pKa = 11.84 SNGSSSLMTEE74 pKa = 4.11 DD75 pKa = 4.08 QAASDD80 pKa = 4.0 LDD82 pKa = 3.82 KK83 pKa = 11.17 VYY85 pKa = 10.57 HH86 pKa = 5.57 QLGEE90 pKa = 3.92 ADD92 pKa = 3.17 ITEE95 pKa = 4.32 TSVTDD100 pKa = 3.95 LDD102 pKa = 4.77 HH103 pKa = 8.26 DD104 pKa = 4.06 EE105 pKa = 5.21 LYY107 pKa = 11.19 QNASSAEE114 pKa = 3.95 YY115 pKa = 10.12 SVEE118 pKa = 3.96 LVFPSAVSWSMVNEE132 pKa = 4.18 LFSFEE137 pKa = 4.85 DD138 pKa = 3.55 EE139 pKa = 4.45 SNEE142 pKa = 4.19 VEE144 pKa = 4.15 EE145 pKa = 5.19 ALDD148 pKa = 3.86 GSIDD152 pKa = 4.32 RR153 pKa = 11.84 ILLTASPGSEE163 pKa = 3.69 EE164 pKa = 3.99 LNAVFVSYY172 pKa = 11.32 DD173 pKa = 3.1 EE174 pKa = 4.53 GVVVNSNTNLDD185 pKa = 3.79 KK186 pKa = 11.8 NEE188 pKa = 4.06 IEE190 pKa = 4.27 YY191 pKa = 10.21 EE192 pKa = 4.03 QYY194 pKa = 10.83 LAEE197 pKa = 4.61 HH198 pKa = 6.92 EE199 pKa = 4.58 SMLTHH204 pKa = 7.0 DD205 pKa = 4.65 TDD207 pKa = 3.55 QEE209 pKa = 4.66 GYY211 pKa = 9.27 EE212 pKa = 3.87 KK213 pKa = 10.33 HH214 pKa = 6.8 IYY216 pKa = 9.92 LPQDD220 pKa = 3.15 PGARR224 pKa = 11.84 TNYY227 pKa = 9.24 TYY229 pKa = 11.51 ASTDD233 pKa = 2.89 LSTNEE238 pKa = 3.82 FQQVLIADD246 pKa = 4.07 EE247 pKa = 4.31 NAQNLQTYY255 pKa = 8.43 TSDD258 pKa = 3.26 STEE261 pKa = 4.05 VITDD265 pKa = 3.55 GTRR268 pKa = 11.84 EE269 pKa = 3.81 LRR271 pKa = 11.84 INNVGDD277 pKa = 3.47 YY278 pKa = 10.81 LRR280 pKa = 11.84 YY281 pKa = 10.17 ANPTYY286 pKa = 10.77 SEE288 pKa = 4.11 QTDD291 pKa = 3.96 GNDD294 pKa = 3.25 TSSLISSFEE303 pKa = 3.81 FVNNHH308 pKa = 6.39 AGWSDD313 pKa = 3.31 EE314 pKa = 3.43 YY315 pKa = 10.82 RR316 pKa = 11.84 YY317 pKa = 10.4 YY318 pKa = 11.11 SSSTVDD324 pKa = 4.02 GEE326 pKa = 4.52 TTDD329 pKa = 3.42 TFRR332 pKa = 11.84 MNKK335 pKa = 9.81 GGLPVFRR342 pKa = 11.84 DD343 pKa = 3.72 SNGSDD348 pKa = 3.22 FASIQVSNVGTQTTGYY364 pKa = 10.0 SRR366 pKa = 11.84 PLFDD370 pKa = 5.63 LEE372 pKa = 4.29 NNPVRR377 pKa = 11.84 TGDD380 pKa = 3.58 AGVSTDD386 pKa = 4.06 LPTGEE391 pKa = 4.32 EE392 pKa = 3.96 TLDD395 pKa = 3.69 AVQEE399 pKa = 4.25 QCSSSLIEE407 pKa = 5.48 DD408 pKa = 3.31 IQIGYY413 pKa = 9.63 SISRR417 pKa = 11.84 NGEE420 pKa = 3.38 NSGYY424 pKa = 10.74 YY425 pKa = 10.47 DD426 pKa = 4.17 MEE428 pKa = 4.94 PGWFVRR434 pKa = 11.84 CDD436 pKa = 3.85 GSWSPVEE443 pKa = 5.12 FGGDD447 pKa = 3.49 DD448 pKa = 3.62 NEE450 pKa = 4.67 EE451 pKa = 4.08 GGEE454 pKa = 4.0
Molecular weight: 50.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.935
Patrickios 1.125
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A1H2WHJ4|A0A1H2WHJ4_9BACI Glutamate synthase domain-containing protein 2 OS=Marinococcus luteus OX=1122204 GN=SAMN05421781_2414 PE=3 SV=1
MM1 pKa = 7.67 GKK3 pKa = 7.99 PTFTPNNRR11 pKa = 11.84 KK12 pKa = 9.22 RR13 pKa = 11.84 KK14 pKa = 8.37 KK15 pKa = 8.69 VHH17 pKa = 5.54 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MGTKK26 pKa = 10.01 NGRR29 pKa = 11.84 QVLKK33 pKa = 10.19 NRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.66 GRR40 pKa = 11.84 KK41 pKa = 8.69 VLSAA45 pKa = 4.05
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2897
0
2897
842470
29
1535
290.8
32.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.352 ± 0.056
0.639 ± 0.012
5.211 ± 0.04
8.465 ± 0.061
4.229 ± 0.037
7.36 ± 0.044
2.26 ± 0.026
6.408 ± 0.046
5.256 ± 0.047
9.206 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.913 ± 0.022
3.719 ± 0.032
3.948 ± 0.023
3.953 ± 0.029
4.807 ± 0.037
6.034 ± 0.037
5.483 ± 0.028
7.198 ± 0.044
1.185 ± 0.018
3.374 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here