Hubei tombus-like virus 34

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT0|A0A1L3KGT0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 34 OX=1923282 PE=4 SV=1
MM1 pKa = 7.24PTEE4 pKa = 3.88KK5 pKa = 10.65LYY7 pKa = 10.93DD8 pKa = 3.78EE9 pKa = 5.41RR10 pKa = 11.84DD11 pKa = 3.46GTNWDD16 pKa = 3.25STMQEE21 pKa = 3.22PHH23 pKa = 5.86MRR25 pKa = 11.84FEE27 pKa = 4.23ASVYY31 pKa = 10.74SLFDD35 pKa = 3.3AVIGQRR41 pKa = 11.84HH42 pKa = 5.5LQRR45 pKa = 11.84STHH48 pKa = 5.99CKK50 pKa = 8.48GTIATRR56 pKa = 11.84DD57 pKa = 3.7YY58 pKa = 10.32IIKK61 pKa = 10.55YY62 pKa = 4.66VTRR65 pKa = 11.84WKK67 pKa = 10.53RR68 pKa = 11.84LSGDD72 pKa = 2.86WNTSVGNSIISMAICITAILSLPSHH97 pKa = 6.62LRR99 pKa = 11.84PHH101 pKa = 6.36RR102 pKa = 11.84VAAFFHH108 pKa = 6.69GDD110 pKa = 3.45DD111 pKa = 3.64YY112 pKa = 11.78LAIYY116 pKa = 9.02HH117 pKa = 5.46YY118 pKa = 10.33HH119 pKa = 7.2RR120 pKa = 11.84SPPPQVLNTALADD133 pKa = 3.85LEE135 pKa = 4.32KK136 pKa = 10.93SLGITPVRR144 pKa = 11.84GVFRR148 pKa = 11.84DD149 pKa = 3.74PLLVEE154 pKa = 4.51YY155 pKa = 10.24ISMSVWPCYY164 pKa = 10.44DD165 pKa = 2.74GTYY168 pKa = 10.53FFAPKK173 pKa = 10.3LSNLFVRR180 pKa = 11.84LFFSTRR186 pKa = 11.84PLSQHH191 pKa = 5.74TADD194 pKa = 4.92DD195 pKa = 3.85VCATIAALAPHH206 pKa = 6.58FVGCEE211 pKa = 3.33PAEE214 pKa = 4.1RR215 pKa = 11.84FFAAHH220 pKa = 5.51RR221 pKa = 11.84RR222 pKa = 11.84AWIARR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 11.84GIKK232 pKa = 9.78HH233 pKa = 6.57AGNQFRR239 pKa = 11.84DD240 pKa = 3.73CHH242 pKa = 7.07SLEE245 pKa = 4.2KK246 pKa = 11.09LEE248 pKa = 4.77IDD250 pKa = 3.81HH251 pKa = 6.41TPKK254 pKa = 10.41VHH256 pKa = 5.92WAYY259 pKa = 10.7GFAHH263 pKa = 7.76KK264 pKa = 10.92YY265 pKa = 9.82HH266 pKa = 6.15MPITALHH273 pKa = 6.0VEE275 pKa = 4.56VEE277 pKa = 4.45CGSAALLRR285 pKa = 11.84HH286 pKa = 6.2PAYY289 pKa = 10.54DD290 pKa = 3.35HH291 pKa = 7.1LFEE294 pKa = 6.05CEE296 pKa = 4.29HH297 pKa = 7.09LDD299 pKa = 3.31PDD301 pKa = 3.8VRR303 pKa = 11.84MGASATRR310 pKa = 11.84DD311 pKa = 2.97II312 pKa = 4.9

Molecular weight: 35.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGM2|A0A1L3KGM2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 34 OX=1923282 PE=4 SV=1
MM1 pKa = 7.49LEE3 pKa = 3.46WGRR6 pKa = 11.84PRR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 3.89TSDD13 pKa = 2.93SDD15 pKa = 3.44LRR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.82IEE21 pKa = 3.99TMATNLARR29 pKa = 11.84AIALPGKK36 pKa = 9.19HH37 pKa = 6.08APLRR41 pKa = 11.84FPSFPALEE49 pKa = 4.21RR50 pKa = 11.84TAVMAFNASMPFSIGADD67 pKa = 3.39STKK70 pKa = 10.79VILTRR75 pKa = 11.84QAALPLWGEE84 pKa = 4.21TKK86 pKa = 10.89APGGEE91 pKa = 4.1NAALTYY97 pKa = 10.48GVVYY101 pKa = 10.64SCTGVSAVNTQGGIYY116 pKa = 9.45TEE118 pKa = 4.31AVDD121 pKa = 6.29AIGGWHH127 pKa = 5.83QSVTYY132 pKa = 10.11GLSAPVMAVPGGTWPTSYY150 pKa = 10.53PVVAFDD156 pKa = 4.98AATGDD161 pKa = 4.35LPWVYY166 pKa = 10.1CPNGGKK172 pKa = 9.62VLITSNAMAFAHH184 pKa = 6.63NDD186 pKa = 3.44NIQLEE191 pKa = 4.67RR192 pKa = 11.84WTGPGQSEE200 pKa = 5.22PITLTAAHH208 pKa = 6.25TASLNLGGGSYY219 pKa = 11.04FDD221 pKa = 3.39VTANAWYY228 pKa = 10.0RR229 pKa = 11.84FRR231 pKa = 11.84SSSIQDD237 pKa = 3.36TTTGSVLKK245 pKa = 7.51FTPVYY250 pKa = 10.44SLGVLLSDD258 pKa = 4.13QAPTFTGAAVNGTWAIPTTNDD279 pKa = 2.56QRR281 pKa = 11.84RR282 pKa = 11.84FLLPLTVSPEE292 pKa = 3.94FSNSHH297 pKa = 6.19LPWAATRR304 pKa = 11.84LTAVGALFTNTTKK317 pKa = 10.7VLNKK321 pKa = 9.95EE322 pKa = 4.28GTVLWGRR329 pKa = 11.84INPVVYY335 pKa = 10.48DD336 pKa = 3.83PFIVTSTTIQNLHH349 pKa = 6.37PAEE352 pKa = 4.1KK353 pKa = 10.16AYY355 pKa = 11.1LDD357 pKa = 4.54LEE359 pKa = 4.95HH360 pKa = 6.68GTYY363 pKa = 10.29AYY365 pKa = 10.38NPPSTDD371 pKa = 3.25LADD374 pKa = 3.83FTPYY378 pKa = 10.36ILRR381 pKa = 11.84LLPNSASNSAIWPVYY396 pKa = 10.28RR397 pKa = 11.84LDD399 pKa = 3.48NRR401 pKa = 11.84AFAAVGFFNDD411 pKa = 3.99PDD413 pKa = 4.28GGTNLAVNLDD423 pKa = 3.46YY424 pKa = 10.97HH425 pKa = 7.98IEE427 pKa = 4.02FRR429 pKa = 11.84TSSTLFQIGVSTMPLEE445 pKa = 4.5ALHH448 pKa = 6.49AAQIVLLKK456 pKa = 10.92AGFFFHH462 pKa = 7.08NEE464 pKa = 3.4DD465 pKa = 3.36HH466 pKa = 6.55GAVIARR472 pKa = 11.84IVKK475 pKa = 9.39FMAGMHH481 pKa = 6.92PLLSMAVPIANGLIGASSYY500 pKa = 11.6ALSSKK505 pKa = 9.96PNQSHH510 pKa = 5.78TPAATSGQGAGMVSVAAPPRR530 pKa = 11.84PRR532 pKa = 11.84RR533 pKa = 11.84AAARR537 pKa = 11.84RR538 pKa = 11.84PPARR542 pKa = 11.84ARR544 pKa = 11.84PRR546 pKa = 11.84PRR548 pKa = 11.84RR549 pKa = 11.84AAPPPPPPPQKK560 pKa = 10.62KK561 pKa = 9.6KK562 pKa = 10.91GKK564 pKa = 9.06LASGLDD570 pKa = 3.58MYY572 pKa = 11.16LSRR575 pKa = 11.84KK576 pKa = 8.94KK577 pKa = 10.5RR578 pKa = 3.36

Molecular weight: 62.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1665

312

775

555.0

60.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.793 ± 0.973

1.502 ± 0.484

4.865 ± 0.52

4.144 ± 0.607

3.844 ± 0.464

6.426 ± 0.592

3.183 ± 0.924

3.844 ± 0.599

3.483 ± 0.224

8.228 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.219

3.724 ± 0.509

6.486 ± 0.938

2.763 ± 0.352

6.667 ± 0.409

6.066 ± 0.619

7.147 ± 0.565

7.327 ± 0.917

1.802 ± 0.072

3.243 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski