Gordonia phage Marteena
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516KTT5|A0A516KTT5_9CAUD Tail terminator OS=Gordonia phage Marteena OX=2590930 GN=13 PE=4 SV=1
MM1 pKa = 7.41 AVAACLSLAVSAGCSSEE18 pKa = 4.26 SGSNGATSAATSSAAASAPSVAITSWATWAQNYY51 pKa = 7.56 QSEE54 pKa = 4.55 IEE56 pKa = 4.28 DD57 pKa = 3.93 CAGEE61 pKa = 4.08 TDD63 pKa = 3.65 SCFTQNHH70 pKa = 4.41 ATMAEE75 pKa = 4.07 MRR77 pKa = 11.84 QSIIDD82 pKa = 3.63 NNLPIDD88 pKa = 3.97 GTNSGIVNPMQLYY101 pKa = 9.65 QEE103 pKa = 4.89 TYY105 pKa = 11.4 AKK107 pKa = 9.54 FTEE110 pKa = 4.5 QNCIGGPQDD119 pKa = 3.37 MNSANVVCNVKK130 pKa = 9.84 RR131 pKa = 11.84 DD132 pKa = 3.52 QMRR135 pKa = 11.84 VFGSQVKK142 pKa = 9.38 EE143 pKa = 4.12 QLDD146 pKa = 3.8 SMATGG151 pKa = 3.46
Molecular weight: 15.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.982
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.923
ProMoST 4.24
Dawson 4.062
Bjellqvist 4.228
Wikipedia 3.973
Rodwell 3.935
Grimsley 3.834
Solomon 4.05
Lehninger 4.012
Nozaki 4.19
DTASelect 4.342
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.215
Patrickios 0.604
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.116
Protein with the highest isoelectric point:
>tr|A0A516KTU2|A0A516KTU2_9CAUD Major tail protein OS=Gordonia phage Marteena OX=2590930 GN=14 PE=4 SV=1
MM1 pKa = 7.63 SVRR4 pKa = 11.84 LRR6 pKa = 11.84 FNIDD10 pKa = 2.36 GFYY13 pKa = 10.89 DD14 pKa = 3.72 LRR16 pKa = 11.84 RR17 pKa = 11.84 DD18 pKa = 3.61 PGIVAEE24 pKa = 4.75 EE25 pKa = 3.86 EE26 pKa = 4.87 AIAQQIADD34 pKa = 4.32 RR35 pKa = 11.84 ANSIGKK41 pKa = 8.2 GTYY44 pKa = 10.12 AVGSRR49 pKa = 11.84 QGRR52 pKa = 11.84 KK53 pKa = 9.29 APQGRR58 pKa = 11.84 WRR60 pKa = 11.84 TTVVTADD67 pKa = 3.27 ARR69 pKa = 11.84 AMANNARR76 pKa = 11.84 NNTLIRR82 pKa = 11.84 AMEE85 pKa = 3.98
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.648
IPC_protein 10.833
Toseland 10.818
ProMoST 10.906
Dawson 10.891
Bjellqvist 10.73
Wikipedia 11.228
Rodwell 10.804
Grimsley 10.95
Solomon 11.169
Lehninger 11.111
Nozaki 10.789
DTASelect 10.73
Thurlkill 10.818
EMBOSS 11.257
Sillero 10.847
Patrickios 10.599
IPC_peptide 11.169
IPC2_peptide 9.94
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
16230
39
1884
210.8
22.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.898 ± 0.587
0.906 ± 0.155
7.012 ± 0.29
5.564 ± 0.267
2.637 ± 0.122
8.349 ± 0.523
2.187 ± 0.249
4.553 ± 0.218
3.124 ± 0.202
7.813 ± 0.211
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.206 ± 0.129
3.025 ± 0.248
5.533 ± 0.323
3.869 ± 0.44
7.757 ± 0.463
5.705 ± 0.267
6.845 ± 0.284
6.888 ± 0.261
2.002 ± 0.178
2.126 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here