Sphingomonas sp. EC-HK361

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E7XR96|A0A5E7XR96_9SPHN 50S ribosomal protein L33 OS=Sphingomonas sp. EC-HK361 OX=2038397 GN=rpmG PE=3 SV=1
MM1 pKa = 7.69ADD3 pKa = 3.42YY4 pKa = 11.22YY5 pKa = 10.36NTLGSSIANGTTGRR19 pKa = 11.84DD20 pKa = 3.11LFFPFTKK27 pKa = 10.48DD28 pKa = 3.07PNFDD32 pKa = 3.59HH33 pKa = 6.92LRR35 pKa = 11.84ADD37 pKa = 3.39ATLSQLVWSTGILTGSGTAYY57 pKa = 10.43QIIAPNVQISMDD69 pKa = 4.08LLNGGEE75 pKa = 4.37GTDD78 pKa = 3.04IVYY81 pKa = 10.85ASNVADD87 pKa = 3.59ALFYY91 pKa = 11.42NNGAIAGGFGGFSNIEE107 pKa = 4.05QFWMGDD113 pKa = 2.94GDD115 pKa = 5.82DD116 pKa = 5.21IIDD119 pKa = 4.09LTAHH123 pKa = 6.61GAGGVDD129 pKa = 3.65YY130 pKa = 10.87AKK132 pKa = 10.63AAAIHH137 pKa = 5.57GQGGNDD143 pKa = 3.75IIIGGAGKK151 pKa = 8.58DD152 pKa = 3.66TLEE155 pKa = 4.73GDD157 pKa = 3.7AGNDD161 pKa = 3.57IIFGWRR167 pKa = 11.84GSDD170 pKa = 3.44TIFGGTGDD178 pKa = 4.37DD179 pKa = 4.74LLYY182 pKa = 11.19GDD184 pKa = 5.62DD185 pKa = 4.73LGFNGIAGDD194 pKa = 3.82DD195 pKa = 4.25TIDD198 pKa = 3.63GGAGNDD204 pKa = 3.05ILYY207 pKa = 10.5GGRR210 pKa = 11.84GSDD213 pKa = 4.0KK214 pKa = 9.3MTGGDD219 pKa = 4.91DD220 pKa = 3.81NDD222 pKa = 3.64ILYY225 pKa = 9.93GQAGGDD231 pKa = 3.61NMSGGSGDD239 pKa = 4.01DD240 pKa = 3.87LLYY243 pKa = 11.2GDD245 pKa = 5.82DD246 pKa = 6.16ADD248 pKa = 4.14TTSNDD253 pKa = 3.82TLNGDD258 pKa = 3.66AGADD262 pKa = 3.38RR263 pKa = 11.84LYY265 pKa = 11.28GGGGDD270 pKa = 3.86DD271 pKa = 3.82EE272 pKa = 6.14LYY274 pKa = 10.98GGSGDD279 pKa = 4.87DD280 pKa = 3.99YY281 pKa = 11.7LDD283 pKa = 4.0GGTGNDD289 pKa = 3.88YY290 pKa = 11.25VHH292 pKa = 7.03GGAGNDD298 pKa = 3.89TIVAGAGNDD307 pKa = 3.87IIDD310 pKa = 4.19GSADD314 pKa = 2.97IDD316 pKa = 3.79TVVFAGNRR324 pKa = 11.84ADD326 pKa = 3.69YY327 pKa = 10.86QFALQTDD334 pKa = 4.46GSFIATDD341 pKa = 3.44MRR343 pKa = 11.84GGSPEE348 pKa = 3.8GTKK351 pKa = 9.94TIRR354 pKa = 11.84NVEE357 pKa = 4.28FFAFADD363 pKa = 3.94TTVSSTLLNYY373 pKa = 10.67LPVITSNGSGAAASLDD389 pKa = 3.17IDD391 pKa = 4.05EE392 pKa = 5.05NATLVTTVTATDD404 pKa = 3.15QDD406 pKa = 3.52AGQTLTFTIAGGADD420 pKa = 2.98AALFTVDD427 pKa = 3.99PVTGVLSFITAPNFEE442 pKa = 4.79SPSDD446 pKa = 3.79ANGDD450 pKa = 3.2NVYY453 pKa = 10.48RR454 pKa = 11.84VVVAVNDD461 pKa = 4.03GNGGVDD467 pKa = 3.55TQDD470 pKa = 3.26LSVNVRR476 pKa = 11.84DD477 pKa = 4.31VPDD480 pKa = 3.8GFAPVITSNGGGATAAITVNEE501 pKa = 4.14NLTAVTTVTATDD513 pKa = 3.57ADD515 pKa = 4.32GPTLAFAIIGGADD528 pKa = 3.18AALFAIDD535 pKa = 4.43PATGALTFKK544 pKa = 10.41NAPDD548 pKa = 3.77FEE550 pKa = 4.76NPGDD554 pKa = 3.98ANHH557 pKa = 7.2DD558 pKa = 3.51NVYY561 pKa = 10.92DD562 pKa = 3.98VVVQASDD569 pKa = 3.62GNNTDD574 pKa = 3.26SQALSVTVGNVNDD587 pKa = 3.95NAPALTSFGGALAASFTVNEE607 pKa = 4.36NVTAATTIAASDD619 pKa = 3.78ADD621 pKa = 4.33GDD623 pKa = 4.21ALSYY627 pKa = 10.43TITGGADD634 pKa = 2.9AALFAVDD641 pKa = 3.78AATGRR646 pKa = 11.84LTFITPPDD654 pKa = 3.61YY655 pKa = 10.2EE656 pKa = 5.0APGDD660 pKa = 3.87SDD662 pKa = 3.62HH663 pKa = 7.38DD664 pKa = 4.13RR665 pKa = 11.84IYY667 pKa = 10.93QVVVSASDD675 pKa = 3.47GTNSVAQALSITIANVNDD693 pKa = 3.65NAPVITSNGGGGTASLGIAEE713 pKa = 5.14NGTAVTIVTATDD725 pKa = 3.39ADD727 pKa = 4.23GTVPTYY733 pKa = 10.87AIAGGADD740 pKa = 3.06AALFTIDD747 pKa = 4.22PATGALSFLSAPDD760 pKa = 3.82FEE762 pKa = 6.09HH763 pKa = 7.51PLDD766 pKa = 4.46ADD768 pKa = 3.65ANGIYY773 pKa = 9.89QVTVRR778 pKa = 11.84ATDD781 pKa = 3.44GLNVVDD787 pKa = 4.41QLLSVSVTDD796 pKa = 3.47VNEE799 pKa = 3.91VGRR802 pKa = 11.84TITGTSGNNTISPTTTVVGYY822 pKa = 7.47QTTALGDD829 pKa = 3.7TIYY832 pKa = 11.41ALAGNDD838 pKa = 4.05IIDD841 pKa = 4.14GGAGADD847 pKa = 3.9YY848 pKa = 10.48MDD850 pKa = 4.95GGAGNDD856 pKa = 3.35TFYY859 pKa = 11.74VDD861 pKa = 3.46TFSDD865 pKa = 3.66DD866 pKa = 3.9GYY868 pKa = 11.22AGNDD872 pKa = 3.32DD873 pKa = 4.49RR874 pKa = 11.84VIEE877 pKa = 4.03LAGGGTDD884 pKa = 3.68LVYY887 pKa = 11.06ASVSYY892 pKa = 10.8RR893 pKa = 11.84LADD896 pKa = 3.39QVEE899 pKa = 4.18NLTLTGTAALNGYY912 pKa = 10.21GNDD915 pKa = 3.54LANIITGNDD924 pKa = 2.96AANRR928 pKa = 11.84LEE930 pKa = 4.55GGLGADD936 pKa = 3.68TLYY939 pKa = 11.55GMGGNDD945 pKa = 3.7TLDD948 pKa = 3.8GGDD951 pKa = 3.91GNDD954 pKa = 3.41RR955 pKa = 11.84LYY957 pKa = 11.36GGDD960 pKa = 3.73GTDD963 pKa = 3.38TLIGGTGDD971 pKa = 3.49DD972 pKa = 4.27TLDD975 pKa = 3.78GGTGADD981 pKa = 3.6TMTGGDD987 pKa = 4.22GNDD990 pKa = 3.1SYY992 pKa = 12.01YY993 pKa = 11.05VDD995 pKa = 4.2DD996 pKa = 5.85AGDD999 pKa = 3.63QVVEE1003 pKa = 4.18LANGGTTDD1011 pKa = 3.99QIFSAITYY1019 pKa = 7.53SLPAEE1024 pKa = 4.3VEE1026 pKa = 4.14KK1027 pKa = 10.44LTLTGTDD1034 pKa = 3.89AIDD1037 pKa = 3.79GRR1039 pKa = 11.84GNEE1042 pKa = 4.47LNNTITGNGANNGLWGGAGNDD1063 pKa = 3.89TLSGNAGDD1071 pKa = 4.98DD1072 pKa = 3.5RR1073 pKa = 11.84LFGEE1077 pKa = 5.45DD1078 pKa = 5.3GLDD1081 pKa = 3.53TLDD1084 pKa = 4.18GGVGNDD1090 pKa = 3.94RR1091 pKa = 11.84LDD1093 pKa = 3.88GGAGADD1099 pKa = 3.68TLLGKK1104 pKa = 10.31AGNDD1108 pKa = 3.61TLIGGAGKK1116 pKa = 8.54DD1117 pKa = 3.47TLTGGTEE1124 pKa = 3.76ADD1126 pKa = 3.14TFVFNFGDD1134 pKa = 3.64TTLNSASYY1142 pKa = 11.18DD1143 pKa = 3.65RR1144 pKa = 11.84ITDD1147 pKa = 4.24FKK1149 pKa = 11.21ASEE1152 pKa = 4.3GDD1154 pKa = 4.06VIDD1157 pKa = 5.0LDD1159 pKa = 4.22VVNGSLAPADD1169 pKa = 4.04FTATTIATNNFADD1182 pKa = 4.09ALTAAKK1188 pKa = 8.54LTQSAGHH1195 pKa = 5.64ITFVAGATDD1204 pKa = 4.41GWLFWDD1210 pKa = 4.38GNGDD1214 pKa = 3.83GVFDD1218 pKa = 4.41QSVLLSGANTQSAIDD1233 pKa = 3.94PLHH1236 pKa = 6.27IVV1238 pKa = 3.39

Molecular weight: 124.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E7YES7|A0A5E7YES7_9SPHN 30S ribosomal protein S4 OS=Sphingomonas sp. EC-HK361 OX=2038397 GN=rpsD PE=3 SV=1
MM1 pKa = 7.59ARR3 pKa = 11.84TSLPYY8 pKa = 10.37RR9 pKa = 11.84ITAQALQSRR18 pKa = 11.84ARR20 pKa = 11.84APRR23 pKa = 11.84AARR26 pKa = 11.84SISAARR32 pKa = 11.84VVFAPRR38 pKa = 11.84LAVGRR43 pKa = 11.84PGSS46 pKa = 3.44

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3256

0

3256

1014211

20

1842

311.5

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.165 ± 0.082

0.75 ± 0.015

6.172 ± 0.031

4.909 ± 0.04

3.462 ± 0.028

8.975 ± 0.041

1.946 ± 0.026

4.977 ± 0.025

2.872 ± 0.037

9.581 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.021

2.375 ± 0.032

5.395 ± 0.033

2.951 ± 0.028

7.604 ± 0.049

4.964 ± 0.036

5.609 ± 0.038

7.348 ± 0.033

1.407 ± 0.019

2.183 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski