Amino acid dipepetide frequency for Sphingomonas sp. EC-HK361

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.167AlaAla: 21.167 ± 0.224	1.098AlaCys: 1.098 ± 0.039	8.371AlaAsp: 8.371 ± 0.098	7.348AlaGlu: 7.348 ± 0.103	4.58AlaPhe: 4.58 ± 0.069	12.241AlaGly: 12.241 ± 0.124	2.541AlaHis: 2.541 ± 0.051	7.613AlaIle: 7.613 ± 0.097	4.409AlaLys: 4.409 ± 0.083	14.486AlaLeu: 14.486 ± 0.139	4.071AlaMet: 4.071 ± 0.077	3.335AlaAsn: 3.335 ± 0.073	6.824AlaPro: 6.824 ± 0.1	4.593AlaGln: 4.593 ± 0.082	10.855AlaArg: 10.855 ± 0.128	6.757AlaSer: 6.757 ± 0.082	7.64AlaThr: 7.64 ± 0.083	9.278AlaVal: 9.278 ± 0.113	1.75AlaTrp: 1.75 ± 0.052	2.696AlaTyr: 2.696 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	1.032CysAla: 1.032 ± 0.031	0.096CysCys: 0.096 ± 0.01	0.528CysAsp: 0.528 ± 0.023	0.363CysGlu: 0.363 ± 0.02	0.254CysPhe: 0.254 ± 0.016	0.834CysGly: 0.834 ± 0.032	0.205CysHis: 0.205 ± 0.014	0.304CysIle: 0.304 ± 0.017	0.159CysLys: 0.159 ± 0.013	0.642CysLeu: 0.642 ± 0.028	0.125CysMet: 0.125 ± 0.013	0.177CysAsn: 0.177 ± 0.014	0.443CysPro: 0.443 ± 0.022	0.193CysGln: 0.193 ± 0.015	0.575CysArg: 0.575 ± 0.022	0.4CysSer: 0.4 ± 0.02	0.387CysThr: 0.387 ± 0.02	0.517CysVal: 0.517 ± 0.026	0.105CysTrp: 0.105 ± 0.012	0.157CysTyr: 0.157 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.892AspAla: 8.892 ± 0.105	0.479AspCys: 0.479 ± 0.026	3.595AspAsp: 3.595 ± 0.068	3.24AspGlu: 3.24 ± 0.062	2.131AspPhe: 2.131 ± 0.041	5.832AspGly: 5.832 ± 0.087	1.379AspHis: 1.379 ± 0.039	2.795AspIle: 2.795 ± 0.055	1.558AspLys: 1.558 ± 0.043	5.853AspLeu: 5.853 ± 0.091	1.325AspMet: 1.325 ± 0.035	1.236AspAsn: 1.236 ± 0.039	3.911AspPro: 3.911 ± 0.064	1.865AspGln: 1.865 ± 0.048	5.427AspArg: 5.427 ± 0.088	2.355AspSer: 2.355 ± 0.051	3.046AspThr: 3.046 ± 0.057	4.121AspVal: 4.121 ± 0.063	1.097AspTrp: 1.097 ± 0.042	1.585AspTyr: 1.585 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	7.475GluAla: 7.475 ± 0.098	0.272GluCys: 0.272 ± 0.019	2.565GluAsp: 2.565 ± 0.062	2.362GluGlu: 2.362 ± 0.068	1.282GluPhe: 1.282 ± 0.038	4.194GluGly: 4.194 ± 0.061	1.044GluHis: 1.044 ± 0.029	2.648GluIle: 2.648 ± 0.059	1.695GluLys: 1.695 ± 0.045	4.194GluLeu: 4.194 ± 0.076	1.279GluMet: 1.279 ± 0.042	1.161GluAsn: 1.161 ± 0.032	2.357GluPro: 2.357 ± 0.052	1.737GluGln: 1.737 ± 0.047	4.765GluArg: 4.765 ± 0.082	2.09GluSer: 2.09 ± 0.053	3.104GluThr: 3.104 ± 0.058	3.299GluVal: 3.299 ± 0.066	0.676GluTrp: 0.676 ± 0.024	0.887GluTyr: 0.887 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	5.132PheAla: 5.132 ± 0.074	0.258PheCys: 0.258 ± 0.015	2.743PheAsp: 2.743 ± 0.056	1.847PheGlu: 1.847 ± 0.05	1.203PhePhe: 1.203 ± 0.039	3.62PheGly: 3.62 ± 0.061	0.688PheHis: 0.688 ± 0.025	1.33PheIle: 1.33 ± 0.041	0.803PheLys: 0.803 ± 0.03	2.988PheLeu: 2.988 ± 0.058	0.663PheMet: 0.663 ± 0.026	0.872PheAsn: 0.872 ± 0.037	1.503PhePro: 1.503 ± 0.041	0.867PheGln: 0.867 ± 0.025	2.197PheArg: 2.197 ± 0.051	1.751PheSer: 1.751 ± 0.045	2.031PheThr: 2.031 ± 0.048	2.729PheVal: 2.729 ± 0.056	0.506PheTrp: 0.506 ± 0.025	0.888PheTyr: 0.888 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	10.732GlyAla: 10.732 ± 0.127	0.832GlyCys: 0.832 ± 0.033	5.424GlyAsp: 5.424 ± 0.075	4.542GlyGlu: 4.542 ± 0.07	3.682GlyPhe: 3.682 ± 0.062	8.446GlyGly: 8.446 ± 0.132	1.841GlyHis: 1.841 ± 0.052	4.503GlyIle: 4.503 ± 0.073	3.163GlyLys: 3.163 ± 0.064	8.343GlyLeu: 8.343 ± 0.1	2.267GlyMet: 2.267 ± 0.05	2.19GlyAsn: 2.19 ± 0.058	3.571GlyPro: 3.571 ± 0.063	2.843GlyGln: 2.843 ± 0.061	6.795GlyArg: 6.795 ± 0.095	4.696GlySer: 4.696 ± 0.074	5.162GlyThr: 5.162 ± 0.093	6.682GlyVal: 6.682 ± 0.088	1.661GlyTrp: 1.661 ± 0.044	2.373GlyTyr: 2.373 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.634HisAla: 2.634 ± 0.062	0.203HisCys: 0.203 ± 0.013	1.247HisAsp: 1.247 ± 0.035	0.996HisGlu: 0.996 ± 0.033	0.785HisPhe: 0.785 ± 0.028	1.902HisGly: 1.902 ± 0.051	0.56HisHis: 0.56 ± 0.026	0.88HisIle: 0.88 ± 0.03	0.384HisLys: 0.384 ± 0.021	1.828HisLeu: 1.828 ± 0.05	0.379HisMet: 0.379 ± 0.019	0.407HisAsn: 0.407 ± 0.018	1.333HisPro: 1.333 ± 0.035	0.49HisGln: 0.49 ± 0.024	1.602HisArg: 1.602 ± 0.051	0.803HisSer: 0.803 ± 0.03	0.685HisThr: 0.685 ± 0.026	1.498HisVal: 1.498 ± 0.044	0.321HisTrp: 0.321 ± 0.019	0.525HisTyr: 0.525 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	8.572IleAla: 8.572 ± 0.119	0.372IleCys: 0.372 ± 0.019	4.333IleAsp: 4.333 ± 0.069	3.313IleGlu: 3.313 ± 0.057	1.434IlePhe: 1.434 ± 0.044	5.287IleGly: 5.287 ± 0.085	0.82IleHis: 0.82 ± 0.028	1.796IleIle: 1.796 ± 0.048	1.022IleLys: 1.022 ± 0.035	3.869IleLeu: 3.869 ± 0.07	0.766IleMet: 0.766 ± 0.03	1.139IleAsn: 1.139 ± 0.031	2.215IlePro: 2.215 ± 0.05	1.145IleGln: 1.145 ± 0.039	3.186IleArg: 3.186 ± 0.066	2.113IleSer: 2.113 ± 0.054	2.445IleThr: 2.445 ± 0.051	4.485IleVal: 4.485 ± 0.069	0.525IleTrp: 0.525 ± 0.021	0.934IleTyr: 0.934 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.122LysAla: 4.122 ± 0.077	0.151LysCys: 0.151 ± 0.013	1.526LysAsp: 1.526 ± 0.04	0.98LysGlu: 0.98 ± 0.034	0.768LysPhe: 0.768 ± 0.03	2.511LysGly: 2.511 ± 0.064	0.467LysHis: 0.467 ± 0.021	1.292LysIle: 1.292 ± 0.037	0.95LysLys: 0.95 ± 0.044	2.961LysLeu: 2.961 ± 0.056	0.734LysMet: 0.734 ± 0.027	0.639LysAsn: 0.639 ± 0.025	2.075LysPro: 2.075 ± 0.057	0.786LysGln: 0.786 ± 0.026	2.321LysArg: 2.321 ± 0.047	1.537LysSer: 1.537 ± 0.039	1.846LysThr: 1.846 ± 0.049	2.143LysVal: 2.143 ± 0.054	0.352LysTrp: 0.352 ± 0.021	0.559LysTyr: 0.559 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.412LeuAla: 14.412 ± 0.147	0.712LeuCys: 0.712 ± 0.029	6.179LeuAsp: 6.179 ± 0.088	4.111LeuGlu: 4.111 ± 0.065	3.55LeuPhe: 3.55 ± 0.066	8.293LeuGly: 8.293 ± 0.106	1.739LeuHis: 1.739 ± 0.043	4.693LeuIle: 4.693 ± 0.071	2.978LeuLys: 2.978 ± 0.064	8.836LeuLeu: 8.836 ± 0.117	1.989LeuMet: 1.989 ± 0.045	2.272LeuAsn: 2.272 ± 0.05	5.606LeuPro: 5.606 ± 0.079	2.28LeuGln: 2.28 ± 0.051	6.657LeuArg: 6.657 ± 0.107	5.597LeuSer: 5.597 ± 0.086	5.567LeuThr: 5.567 ± 0.081	7.163LeuVal: 7.163 ± 0.091	1.233LeuTrp: 1.233 ± 0.042	1.939LeuTyr: 1.939 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	3.365MetAla: 3.365 ± 0.069	0.152MetCys: 0.152 ± 0.013	0.997MetAsp: 0.997 ± 0.036	0.872MetGlu: 0.872 ± 0.033	0.64MetPhe: 0.64 ± 0.022	1.841MetGly: 1.841 ± 0.044	0.389MetHis: 0.389 ± 0.02	1.381MetIle: 1.381 ± 0.041	0.913MetLys: 0.913 ± 0.035	2.521MetLeu: 2.521 ± 0.058	0.663MetMet: 0.663 ± 0.029	0.601MetAsn: 0.601 ± 0.028	1.446MetPro: 1.446 ± 0.041	0.689MetGln: 0.689 ± 0.023	1.831MetArg: 1.831 ± 0.049	1.261MetSer: 1.261 ± 0.036	2.006MetThr: 2.006 ± 0.041	1.505MetVal: 1.505 ± 0.038	0.221MetTrp: 0.221 ± 0.015	0.268MetTyr: 0.268 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.321AsnAla: 3.321 ± 0.066	0.213AsnCys: 0.213 ± 0.016	1.346AsnAsp: 1.346 ± 0.047	0.93AsnGlu: 0.93 ± 0.031	0.882AsnPhe: 0.882 ± 0.034	2.288AsnGly: 2.288 ± 0.054	0.427AsnHis: 0.427 ± 0.023	1.184AsnIle: 1.184 ± 0.034	0.557AsnLys: 0.557 ± 0.026	2.373AsnLeu: 2.373 ± 0.055	0.491AsnMet: 0.491 ± 0.023	0.586AsnAsn: 0.586 ± 0.029	1.719AsnPro: 1.719 ± 0.039	0.693AsnGln: 0.693 ± 0.03	1.776AsnArg: 1.776 ± 0.042	1.058AsnSer: 1.058 ± 0.045	1.13AsnThr: 1.13 ± 0.04	1.784AsnVal: 1.784 ± 0.055	0.368AsnTrp: 0.368 ± 0.019	0.624AsnTyr: 0.624 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.727ProAla: 7.727 ± 0.105	0.339ProCys: 0.339 ± 0.018	3.883ProAsp: 3.883 ± 0.065	3.04ProGlu: 3.04 ± 0.065	1.885ProPhe: 1.885 ± 0.043	4.857ProGly: 4.857 ± 0.078	1.057ProHis: 1.057 ± 0.033	2.663ProIle: 2.663 ± 0.057	1.562ProLys: 1.562 ± 0.049	4.741ProLeu: 4.741 ± 0.065	1.255ProMet: 1.255 ± 0.038	1.254ProAsn: 1.254 ± 0.039	2.817ProPro: 2.817 ± 0.085	1.715ProGln: 1.715 ± 0.045	3.36ProArg: 3.36 ± 0.065	2.702ProSer: 2.702 ± 0.052	2.865ProThr: 2.865 ± 0.053	4.464ProVal: 4.464 ± 0.07	0.677ProTrp: 0.677 ± 0.024	1.086ProTyr: 1.086 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.117GlnAla: 4.117 ± 0.069	0.196GlnCys: 0.196 ± 0.014	1.356GlnAsp: 1.356 ± 0.037	1.038GlnGlu: 1.038 ± 0.036	1.027GlnPhe: 1.027 ± 0.033	2.37GlnGly: 2.37 ± 0.053	0.567GlnHis: 0.567 ± 0.024	1.621GlnIle: 1.621 ± 0.043	0.793GlnLys: 0.793 ± 0.027	2.843GlnLeu: 2.843 ± 0.063	0.772GlnMet: 0.772 ± 0.026	0.705GlnAsn: 0.705 ± 0.031	1.864GlnPro: 1.864 ± 0.049	1.083GlnGln: 1.083 ± 0.041	2.571GlnArg: 2.571 ± 0.053	1.631GlnSer: 1.631 ± 0.042	1.758GlnThr: 1.758 ± 0.048	2.208GlnVal: 2.208 ± 0.055	0.397GlnTrp: 0.397 ± 0.018	0.592GlnTyr: 0.592 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	10.116ArgAla: 10.116 ± 0.132	0.529ArgCys: 0.529 ± 0.025	4.698ArgAsp: 4.698 ± 0.074	3.852ArgGlu: 3.852 ± 0.069	3.114ArgPhe: 3.114 ± 0.05	5.539ArgGly: 5.539 ± 0.086	1.718ArgHis: 1.718 ± 0.054	4.163ArgIle: 4.163 ± 0.071	1.952ArgLys: 1.952 ± 0.041	8.093ArgLeu: 8.093 ± 0.113	2.045ArgMet: 2.045 ± 0.044	1.712ArgAsn: 1.712 ± 0.05	3.804ArgPro: 3.804 ± 0.065	2.404ArgGln: 2.404 ± 0.05	6.033ArgArg: 6.033 ± 0.1	3.748ArgSer: 3.748 ± 0.063	3.833ArgThr: 3.833 ± 0.063	5.383ArgVal: 5.383 ± 0.075	1.334ArgTrp: 1.334 ± 0.039	1.966ArgTyr: 1.966 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.459SerAla: 6.459 ± 0.08	0.357SerCys: 0.357 ± 0.019	3.026SerAsp: 3.026 ± 0.055	2.227SerGlu: 2.227 ± 0.05	1.955SerPhe: 1.955 ± 0.052	5.293SerGly: 5.293 ± 0.082	0.863SerHis: 0.863 ± 0.032	2.592SerIle: 2.592 ± 0.06	1.296SerLys: 1.296 ± 0.038	4.748SerLeu: 4.748 ± 0.075	1.081SerMet: 1.081 ± 0.04	1.274SerAsn: 1.274 ± 0.035	2.841SerPro: 2.841 ± 0.048	1.333SerGln: 1.333 ± 0.043	3.432SerArg: 3.432 ± 0.063	2.552SerSer: 2.552 ± 0.072	2.631SerThr: 2.631 ± 0.061	3.719SerVal: 3.719 ± 0.063	0.693SerTrp: 0.693 ± 0.024	1.273SerTyr: 1.273 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	7.205ThrAla: 7.205 ± 0.096	0.375ThrCys: 0.375 ± 0.021	3.138ThrAsp: 3.138 ± 0.053	2.277ThrGlu: 2.277 ± 0.049	1.825ThrPhe: 1.825 ± 0.049	5.429ThrGly: 5.429 ± 0.08	0.994ThrHis: 0.994 ± 0.032	3.178ThrIle: 3.178 ± 0.061	1.423ThrLys: 1.423 ± 0.036	6.265ThrLeu: 6.265 ± 0.084	1.264ThrMet: 1.264 ± 0.037	1.339ThrAsn: 1.339 ± 0.047	3.859ThrPro: 3.859 ± 0.084	1.54ThrGln: 1.54 ± 0.043	3.929ThrArg: 3.929 ± 0.051	2.668ThrSer: 2.668 ± 0.075	3.195ThrThr: 3.195 ± 0.068	4.27ThrVal: 4.27 ± 0.073	0.692ThrTrp: 0.692 ± 0.027	1.225ThrTyr: 1.225 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	10.862ValAla: 10.862 ± 0.116	0.53ValCys: 0.53 ± 0.024	4.465ValAsp: 4.465 ± 0.065	4.194ValGlu: 4.194 ± 0.069	2.232ValPhe: 2.232 ± 0.047	5.81ValGly: 5.81 ± 0.078	1.363ValHis: 1.363 ± 0.04	3.656ValIle: 3.656 ± 0.074	2.126ValLys: 2.126 ± 0.057	6.538ValLeu: 6.538 ± 0.086	1.648ValMet: 1.648 ± 0.043	1.867ValAsn: 1.867 ± 0.049	4.043ValPro: 4.043 ± 0.073	2.008ValGln: 2.008 ± 0.048	5.326ValArg: 5.326 ± 0.071	3.977ValSer: 3.977 ± 0.06	4.819ValThr: 4.819 ± 0.069	5.825ValVal: 5.825 ± 0.09	0.837ValTrp: 0.837 ± 0.03	1.351ValTyr: 1.351 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.462TrpAla: 1.462 ± 0.041	0.129TrpCys: 0.129 ± 0.012	0.714TrpAsp: 0.714 ± 0.031	0.514TrpGlu: 0.514 ± 0.024	0.579TrpPhe: 0.579 ± 0.021	0.99TrpGly: 0.99 ± 0.032	0.318TrpHis: 0.318 ± 0.017	0.674TrpIle: 0.674 ± 0.025	0.439TrpLys: 0.439 ± 0.022	1.608TrpLeu: 1.608 ± 0.042	0.367TrpMet: 0.367 ± 0.017	0.4TrpAsn: 0.4 ± 0.019	0.679TrpPro: 0.679 ± 0.031	0.568TrpGln: 0.568 ± 0.028	1.423TrpArg: 1.423 ± 0.039	0.893TrpSer: 0.893 ± 0.032	0.88TrpThr: 0.88 ± 0.029	0.872TrpVal: 0.872 ± 0.031	0.282TrpTrp: 0.282 ± 0.017	0.278TrpTyr: 0.278 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.849TyrAla: 2.849 ± 0.053	0.201TyrCys: 0.201 ± 0.015	1.588TyrAsp: 1.588 ± 0.04	1.045TyrGlu: 1.045 ± 0.031	0.809TyrPhe: 0.809 ± 0.028	2.17TyrGly: 2.17 ± 0.052	0.479TyrHis: 0.479 ± 0.024	0.804TyrIle: 0.804 ± 0.028	0.539TyrLys: 0.539 ± 0.024	2.077TyrLeu: 2.077 ± 0.048	0.381TyrMet: 0.381 ± 0.021	0.583TyrAsn: 0.583 ± 0.032	1.039TyrPro: 1.039 ± 0.033	0.678TyrGln: 0.678 ± 0.029	1.973TyrArg: 1.973 ± 0.051	1.054TyrSer: 1.054 ± 0.037	1.061TyrThr: 1.061 ± 0.036	1.534TyrVal: 1.534 ± 0.035	0.34TyrTrp: 0.34 ± 0.018	0.624TyrTyr: 0.624 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3256 proteins (1014211 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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