Grapevine red blotch virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Grablovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TCQ6|S5TCQ6_9GEMI C3 OS=Grapevine red blotch virus OX=1381007 GN=C3 PE=4 SV=1
MM1 pKa = 6.59VTLNKK6 pKa = 9.91RR7 pKa = 11.84NRR9 pKa = 11.84VLPEE13 pKa = 4.23CDD15 pKa = 3.04SCSSSEE21 pKa = 4.41SSLNDD26 pKa = 3.1IDD28 pKa = 4.91ICGDD32 pKa = 3.54DD33 pKa = 5.24DD34 pKa = 4.85GLGDD38 pKa = 3.74EE39 pKa = 5.23ALDD42 pKa = 4.0AGSVYY47 pKa = 10.77SSSQKK52 pKa = 10.87LLVSVAKK59 pKa = 10.56DD60 pKa = 3.46VLLDD64 pKa = 4.35DD65 pKa = 5.14CDD67 pKa = 3.91STILDD72 pKa = 3.4ISLPSALWFLSQRR85 pKa = 11.84YY86 pKa = 7.02LTCCLRR92 pKa = 11.84KK93 pKa = 9.59EE94 pKa = 4.09LLPLPGISEE103 pKa = 4.25KK104 pKa = 9.8QTVLLRR110 pKa = 11.84QLIRR114 pKa = 11.84RR115 pKa = 11.84VARR118 pKa = 11.84RR119 pKa = 11.84HH120 pKa = 5.76CLFTYY125 pKa = 9.95KK126 pKa = 10.49CEE128 pKa = 3.96EE129 pKa = 4.08WFEE132 pKa = 4.23GCLKK136 pKa = 10.31IKK138 pKa = 10.65KK139 pKa = 9.9DD140 pKa = 3.61GNEE143 pKa = 3.98KK144 pKa = 10.55KK145 pKa = 10.42EE146 pKa = 4.13PPTEE150 pKa = 4.14AEE152 pKa = 5.31KK153 pKa = 10.7KK154 pKa = 10.54AQDD157 pKa = 3.45DD158 pKa = 3.72WEE160 pKa = 4.41EE161 pKa = 4.05FCRR164 pKa = 11.84KK165 pKa = 9.05AACSASS171 pKa = 3.31

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TLE1|S5TLE1_9GEMI Replication-associated protein OS=Grapevine red blotch virus OX=1381007 GN=C1 PE=3 SV=1
MM1 pKa = 7.23VMKK4 pKa = 10.42KK5 pKa = 10.13RR6 pKa = 11.84SRR8 pKa = 11.84QRR10 pKa = 11.84KK11 pKa = 4.54QRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TTGRR21 pKa = 11.84SSAVRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84ARR30 pKa = 11.84PRR32 pKa = 11.84SRR34 pKa = 11.84PCQFAFHH41 pKa = 6.71GNSFGGTPSLFFLTPIALGTGAEE64 pKa = 4.27DD65 pKa = 3.18RR66 pKa = 11.84TGPVLTVSSMYY77 pKa = 10.79LKK79 pKa = 10.86GVVLPSDD86 pKa = 3.44NVTDD90 pKa = 4.3GLHH93 pKa = 7.49DD94 pKa = 3.64IYY96 pKa = 11.1FWIILDD102 pKa = 4.21RR103 pKa = 11.84FPTGTDD109 pKa = 3.31PSVSDD114 pKa = 3.88IFTGSDD120 pKa = 2.91NSGSMIEE127 pKa = 4.08TLTRR131 pKa = 11.84NRR133 pKa = 11.84LNRR136 pKa = 11.84KK137 pKa = 8.14RR138 pKa = 11.84FRR140 pKa = 11.84ILGSKK145 pKa = 9.98KK146 pKa = 10.47LVVGVNKK153 pKa = 10.1KK154 pKa = 8.96PQEE157 pKa = 4.26SLPHH161 pKa = 5.69SRR163 pKa = 11.84AAFNIFQRR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84LVVAFKK179 pKa = 11.03NDD181 pKa = 3.04VSGGGRR187 pKa = 11.84NDD189 pKa = 3.11VEE191 pKa = 4.33RR192 pKa = 11.84NRR194 pKa = 11.84IYY196 pKa = 11.03LSAASASGHH205 pKa = 4.99TFRR208 pKa = 11.84LYY210 pKa = 11.35LNGIVNFYY218 pKa = 10.81NGVIFQQ224 pKa = 3.96

Molecular weight: 25.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1086

123

264

181.0

20.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.512 ± 0.658

2.026 ± 0.905

6.077 ± 1.19

6.169 ± 1.441

4.236 ± 0.752

5.157 ± 0.998

2.486 ± 0.551

5.617 ± 1.055

6.446 ± 0.723

9.576 ± 0.869

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.381 ± 0.314

5.433 ± 1.098

5.433 ± 0.748

3.867 ± 0.785

6.998 ± 1.911

8.471 ± 1.381

6.077 ± 0.83

4.788 ± 1.004

1.381 ± 0.288

3.867 ± 0.711
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski