Methanobacterium lacus (strain AL-21)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobacterium

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0T7P3|F0T7P3_METLA Uncharacterized protein OS=Methanobacterium lacus (strain AL-21) OX=877455 GN=Metbo_1371 PE=4 SV=1
MM1 pKa = 8.26VIMAFMFFGILSTSSAANMTGTWSGSNGVYY31 pKa = 10.56NSTAGPVKK39 pKa = 10.74VNFTEE44 pKa = 4.08KK45 pKa = 10.58HH46 pKa = 5.22NGAATVTATPTGTTNTNTAFWSNASAAGHH75 pKa = 6.11SSLEE79 pKa = 4.28VTLSWNTLTKK89 pKa = 10.73ANNGTITFNFNRR101 pKa = 11.84PVNNPILYY109 pKa = 9.43IDD111 pKa = 4.85RR112 pKa = 11.84IGGNLQNGGTITSSSALLTLLNSGMYY138 pKa = 8.25LTKK141 pKa = 10.84LSGPTSFEE149 pKa = 4.24VTSSTIQRR157 pKa = 11.84TPGVTISSTSTEE169 pKa = 3.67ASMDD173 pKa = 4.0PLGGTAAGTVQIMGTNITSVTFSWWGVGADD203 pKa = 3.64SQADD207 pKa = 3.5GLEE210 pKa = 4.17FLWEE214 pKa = 3.95LEE216 pKa = 4.12APVDD220 pKa = 4.23LSISKK225 pKa = 10.19SDD227 pKa = 3.59SPDD230 pKa = 3.24PVVAGKK236 pKa = 9.98NLTYY240 pKa = 10.49TITVHH245 pKa = 6.35NNGPSSIISSDD256 pKa = 3.33VFTVIDD262 pKa = 3.95NLPAGFTATSYY273 pKa = 10.37TPSEE277 pKa = 4.03GSYY280 pKa = 11.18NPVTGAWTGVTLANGEE296 pKa = 4.47DD297 pKa = 3.52VTLTIIGIVNSTKK310 pKa = 10.43TGTLTNTASVQVPSGITDD328 pKa = 3.81PNTNNNQAPPVITTVDD344 pKa = 3.68GLPTANNDD352 pKa = 3.38SKK354 pKa = 8.73TTPEE358 pKa = 4.09DD359 pKa = 3.75TPISGNLPASDD370 pKa = 4.0PDD372 pKa = 4.0GPVTVVNFVINGTTYY387 pKa = 11.26APGTVNIPGVGKK399 pKa = 10.27IVINSDD405 pKa = 2.54GSYY408 pKa = 10.94LFTPASNYY416 pKa = 9.78NGPVPTITYY425 pKa = 7.28TASDD429 pKa = 3.65NTGNTATGNLNINVTPVNDD448 pKa = 3.86APVGTGDD455 pKa = 4.61SKK457 pKa = 8.75TTPEE461 pKa = 3.97DD462 pKa = 3.41TQATGKK468 pKa = 8.41VTGTDD473 pKa = 3.0VDD475 pKa = 4.0GDD477 pKa = 3.99TLTFAKK483 pKa = 9.77DD484 pKa = 3.27TDD486 pKa = 4.24PSHH489 pKa = 5.82GTVVVNADD497 pKa = 3.01GTYY500 pKa = 10.23TYY502 pKa = 10.12MPNANYY508 pKa = 10.24NGPDD512 pKa = 3.33SFTVIVSDD520 pKa = 4.0GKK522 pKa = 10.77GGSDD526 pKa = 2.49IVTVNINVTPVNDD539 pKa = 3.73APIGNGDD546 pKa = 3.84SKK548 pKa = 9.92TIPEE552 pKa = 4.21DD553 pKa = 3.53TQATGKK559 pKa = 8.41VTGTDD564 pKa = 2.89VDD566 pKa = 4.35GDD568 pKa = 3.43ILTYY572 pKa = 10.02TKK574 pKa = 10.89NSNPTHH580 pKa = 5.37GTVVVNADD588 pKa = 3.01GTYY591 pKa = 10.23TYY593 pKa = 10.12MPNANYY599 pKa = 10.24NGPDD603 pKa = 3.33SFTVIVSDD611 pKa = 4.43GNGGTDD617 pKa = 2.95TVTVTINVTPVNDD630 pKa = 3.81APIGTGDD637 pKa = 3.51SKK639 pKa = 11.42AIPEE643 pKa = 4.13DD644 pKa = 3.76TQATGKK650 pKa = 8.41VTGTDD655 pKa = 3.0VDD657 pKa = 4.43GDD659 pKa = 3.9TLTYY663 pKa = 9.7TKK665 pKa = 10.8NSNPTHH671 pKa = 5.35GTAVVNADD679 pKa = 2.95GTYY682 pKa = 10.08TYY684 pKa = 10.37TPNANYY690 pKa = 10.2NGPDD694 pKa = 3.12SFTIIVSDD702 pKa = 4.02GNGGSDD708 pKa = 3.45VVTVTIDD715 pKa = 3.28VTPVNDD721 pKa = 3.79APVGTGDD728 pKa = 4.02SKK730 pKa = 10.0TIPEE734 pKa = 4.21DD735 pKa = 3.53TQATGKK741 pKa = 8.41VTGTDD746 pKa = 3.0VDD748 pKa = 4.43GDD750 pKa = 3.9TLTYY754 pKa = 9.88TKK756 pKa = 9.54NTNPSHH762 pKa = 5.38GTAVVNADD770 pKa = 2.95GTYY773 pKa = 10.08TYY775 pKa = 10.37TPNANYY781 pKa = 10.2NGPDD785 pKa = 3.35SFTVTVSDD793 pKa = 4.17GNGGSDD799 pKa = 3.45VVTVTIDD806 pKa = 3.28VTPVNDD812 pKa = 3.79APVGTGDD819 pKa = 4.02SKK821 pKa = 10.0TIPEE825 pKa = 4.21DD826 pKa = 3.53TQATGKK832 pKa = 8.41VTGTDD837 pKa = 3.0VDD839 pKa = 4.43GDD841 pKa = 3.9TLTYY845 pKa = 9.92TKK847 pKa = 9.83NTDD850 pKa = 3.54PSHH853 pKa = 5.44GTAVVNADD861 pKa = 2.95GTYY864 pKa = 10.08TYY866 pKa = 10.37TPNANYY872 pKa = 10.2NGPDD876 pKa = 3.35SFTVTVSDD884 pKa = 4.39GNGGTDD890 pKa = 2.95TVTVTINVTPVNDD903 pKa = 3.76APIGNDD909 pKa = 3.26DD910 pKa = 4.22SKK912 pKa = 10.14TIPEE916 pKa = 4.17DD917 pKa = 3.53TQATGKK923 pKa = 8.41VTGTDD928 pKa = 3.0VDD930 pKa = 4.43GDD932 pKa = 3.9TLTYY936 pKa = 9.63TKK938 pKa = 10.78NSNPSHH944 pKa = 5.45GTAVVNADD952 pKa = 2.95GTYY955 pKa = 10.08TYY957 pKa = 10.37TPNANYY963 pKa = 10.2NGPDD967 pKa = 3.12SFTIIVSDD975 pKa = 3.79GKK977 pKa = 11.02GGSDD981 pKa = 3.18VVTVNINVTPVNDD994 pKa = 3.76APIGNDD1000 pKa = 3.26DD1001 pKa = 4.22SKK1003 pKa = 10.14TIPEE1007 pKa = 4.19DD1008 pKa = 3.55TQATGNVTGTDD1019 pKa = 3.17VDD1021 pKa = 4.38GDD1023 pKa = 3.89TLTYY1027 pKa = 9.93TKK1029 pKa = 9.99STDD1032 pKa = 3.57PSHH1035 pKa = 5.75GTAVVNADD1043 pKa = 2.95GTYY1046 pKa = 10.08TYY1048 pKa = 10.37TPNANYY1054 pKa = 10.2NGPDD1058 pKa = 3.35SFTVTVSDD1066 pKa = 4.17GNGGSDD1072 pKa = 3.26VVTVNINVTPVNDD1085 pKa = 3.76APIGNDD1091 pKa = 3.26DD1092 pKa = 4.22SKK1094 pKa = 10.14TIPEE1098 pKa = 4.17DD1099 pKa = 3.53TQATGKK1105 pKa = 8.4VTGTDD1110 pKa = 2.83IDD1112 pKa = 4.74GDD1114 pKa = 3.93TLTYY1118 pKa = 9.92TKK1120 pKa = 9.54NTNPSHH1126 pKa = 5.38GTAVVNADD1134 pKa = 2.95GTYY1137 pKa = 10.08TYY1139 pKa = 10.37TPNANYY1145 pKa = 10.2NGPDD1149 pKa = 3.35SFTVTVSDD1157 pKa = 4.17GNGGSDD1163 pKa = 3.45VVTVTIDD1170 pKa = 3.19VMPVNDD1176 pKa = 4.41APVGTGDD1183 pKa = 3.61SQTIPEE1189 pKa = 4.38DD1190 pKa = 3.58TQATGKK1196 pKa = 8.41VTGTDD1201 pKa = 3.0VDD1203 pKa = 4.43GDD1205 pKa = 3.9TLTYY1209 pKa = 9.92TKK1211 pKa = 9.83NTDD1214 pKa = 3.54PSHH1217 pKa = 5.44GTAVVNADD1225 pKa = 2.95GTYY1228 pKa = 10.08TYY1230 pKa = 10.37TPNANYY1236 pKa = 10.2NGPDD1240 pKa = 3.35SFTVTVSDD1248 pKa = 4.17GNGGSDD1254 pKa = 3.45VVTVTIDD1261 pKa = 3.28VTPVNDD1267 pKa = 3.79APVGTGDD1274 pKa = 4.02SKK1276 pKa = 10.0TIPEE1280 pKa = 4.21DD1281 pKa = 3.53TQATGKK1287 pKa = 10.92VIGTDD1292 pKa = 3.09VDD1294 pKa = 4.37GDD1296 pKa = 3.89TLTYY1300 pKa = 9.63TKK1302 pKa = 10.78NSNPSHH1308 pKa = 5.45GTAVVNADD1316 pKa = 2.95GTYY1319 pKa = 10.08TYY1321 pKa = 10.37TPNANYY1327 pKa = 10.2NGPDD1331 pKa = 3.12SFTIIVSDD1339 pKa = 3.79GKK1341 pKa = 11.02GGSDD1345 pKa = 3.41VVTVTIDD1352 pKa = 3.28VTPVNDD1358 pKa = 3.79APVGTGDD1365 pKa = 3.61SQTIPEE1371 pKa = 4.38DD1372 pKa = 3.58TQATGKK1378 pKa = 8.41VTGTDD1383 pKa = 3.0VDD1385 pKa = 4.43GDD1387 pKa = 3.9TLTYY1391 pKa = 9.91TKK1393 pKa = 9.55NTNPTHH1399 pKa = 5.27GTVVVNADD1407 pKa = 3.0GTYY1410 pKa = 10.08TYY1412 pKa = 10.37TPNANYY1418 pKa = 10.2NGPDD1422 pKa = 3.12SFTIIVSDD1430 pKa = 3.79GKK1432 pKa = 11.04GGSDD1436 pKa = 3.1VVLVNITMTPVDD1448 pKa = 4.44DD1449 pKa = 3.96PVKK1452 pKa = 10.68NVTPTKK1458 pKa = 10.55VKK1460 pKa = 10.36NQTEE1464 pKa = 4.33VGTIPMQRR1472 pKa = 11.84TGVPLPMLFMALVMIFGAFVSNKK1495 pKa = 9.38RR1496 pKa = 11.84KK1497 pKa = 9.99

Molecular weight: 153.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0TA09|F0TA09_METLA Isopropylmalate/isohomocitrate dehydrogenase OS=Methanobacterium lacus (strain AL-21) OX=877455 GN=Metbo_0581 PE=3 SV=1
MM1 pKa = 7.46SRR3 pKa = 11.84NKK5 pKa = 9.93PCAKK9 pKa = 10.18KK10 pKa = 10.51LRR12 pKa = 11.84LSKK15 pKa = 9.8ATKK18 pKa = 8.18QNRR21 pKa = 11.84RR22 pKa = 11.84VPLWVMLKK30 pKa = 9.12TSRR33 pKa = 11.84KK34 pKa = 9.07VRR36 pKa = 11.84THH38 pKa = 5.73PKK40 pKa = 7.11MRR42 pKa = 11.84QWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 11.06VKK50 pKa = 10.52AA51 pKa = 3.54

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2493

0

2493

715694

30

2864

287.1

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.074 ± 0.06

1.125 ± 0.028

5.601 ± 0.045

6.956 ± 0.079

4.145 ± 0.042

6.996 ± 0.058

1.691 ± 0.02

8.915 ± 0.054

7.483 ± 0.057

9.097 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.032

5.752 ± 0.093

3.631 ± 0.03

2.487 ± 0.023

3.383 ± 0.044

6.547 ± 0.046

5.776 ± 0.085

7.175 ± 0.051

0.794 ± 0.018

3.602 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski