Amino acid dipepetide frequency for Methanobacterium lacus (strain AL-21)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.868AlaAla: 4.868 ± 0.1	0.728AlaCys: 0.728 ± 0.042	3.395AlaAsp: 3.395 ± 0.095	3.841AlaGlu: 3.841 ± 0.082	2.386AlaPhe: 2.386 ± 0.063	4.502AlaGly: 4.502 ± 0.097	0.996AlaHis: 0.996 ± 0.044	5.467AlaIle: 5.467 ± 0.113	3.877AlaLys: 3.877 ± 0.084	6.057AlaLeu: 6.057 ± 0.132	1.756AlaMet: 1.756 ± 0.049	2.704AlaAsn: 2.704 ± 0.08	1.822AlaPro: 1.822 ± 0.059	1.496AlaGln: 1.496 ± 0.052	2.106AlaArg: 2.106 ± 0.059	3.851AlaSer: 3.851 ± 0.082	3.251AlaThr: 3.251 ± 0.098	5.361AlaVal: 5.361 ± 0.102	0.411AlaTrp: 0.411 ± 0.021	1.86AlaTyr: 1.86 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.629CysAla: 0.629 ± 0.032	0.17CysCys: 0.17 ± 0.017	0.597CysAsp: 0.597 ± 0.033	0.734CysGlu: 0.734 ± 0.038	0.404CysPhe: 0.404 ± 0.023	1.312CysGly: 1.312 ± 0.059	0.232CysHis: 0.232 ± 0.021	0.768CysIle: 0.768 ± 0.033	0.701CysLys: 0.701 ± 0.035	0.84CysLeu: 0.84 ± 0.043	0.3CysMet: 0.3 ± 0.023	0.546CysAsn: 0.546 ± 0.028	0.798CysPro: 0.798 ± 0.055	0.264CysGln: 0.264 ± 0.019	0.407CysArg: 0.407 ± 0.025	0.749CysSer: 0.749 ± 0.032	0.573CysThr: 0.573 ± 0.034	0.745CysVal: 0.745 ± 0.034	0.059CysTrp: 0.059 ± 0.008	0.426CysTyr: 0.426 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.277AspAla: 3.277 ± 0.076	0.538AspCys: 0.538 ± 0.026	3.116AspAsp: 3.116 ± 0.104	4.58AspGlu: 4.58 ± 0.097	2.349AspPhe: 2.349 ± 0.062	3.672AspGly: 3.672 ± 0.094	1.093AspHis: 1.093 ± 0.042	5.096AspIle: 5.096 ± 0.088	3.746AspLys: 3.746 ± 0.087	5.655AspLeu: 5.655 ± 0.1	1.717AspMet: 1.717 ± 0.048	2.469AspAsn: 2.469 ± 0.065	2.68AspPro: 2.68 ± 0.072	1.276AspGln: 1.276 ± 0.045	1.714AspArg: 1.714 ± 0.054	3.193AspSer: 3.193 ± 0.1	2.542AspThr: 2.542 ± 0.074	4.629AspVal: 4.629 ± 0.089	0.428AspTrp: 0.428 ± 0.03	2.241AspTyr: 2.241 ± 0.071	0.0AspXaa: 0.0 ± 0.0
Glu	3.998GluAla: 3.998 ± 0.089	0.71GluCys: 0.71 ± 0.038	4.357GluAsp: 4.357 ± 0.092	5.677GluGlu: 5.677 ± 0.127	2.782GluPhe: 2.782 ± 0.067	3.925GluGly: 3.925 ± 0.085	1.13GluHis: 1.13 ± 0.044	6.64GluIle: 6.64 ± 0.129	5.867GluLys: 5.867 ± 0.112	6.817GluLeu: 6.817 ± 0.137	2.055GluMet: 2.055 ± 0.062	4.463GluAsn: 4.463 ± 0.096	1.914GluPro: 1.914 ± 0.063	1.488GluGln: 1.488 ± 0.048	2.603GluArg: 2.603 ± 0.071	3.946GluSer: 3.946 ± 0.085	3.968GluThr: 3.968 ± 0.096	4.492GluVal: 4.492 ± 0.101	0.486GluTrp: 0.486 ± 0.026	2.244GluTyr: 2.244 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	2.479PheAla: 2.479 ± 0.065	0.482PheCys: 0.482 ± 0.028	2.328PheAsp: 2.328 ± 0.06	2.686PheGlu: 2.686 ± 0.061	1.805PhePhe: 1.805 ± 0.061	3.05PheGly: 3.05 ± 0.082	0.658PheHis: 0.658 ± 0.034	3.666PheIle: 3.666 ± 0.087	3.179PheLys: 3.179 ± 0.061	3.943PheLeu: 3.943 ± 0.087	1.204PheMet: 1.204 ± 0.044	2.403PheAsn: 2.403 ± 0.06	1.364PhePro: 1.364 ± 0.043	0.992PheGln: 0.992 ± 0.042	1.33PheArg: 1.33 ± 0.045	2.888PheSer: 2.888 ± 0.065	2.349PheThr: 2.349 ± 0.071	2.8PheVal: 2.8 ± 0.068	0.344PheTrp: 0.344 ± 0.022	1.496PheTyr: 1.496 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.661GlyAla: 4.661 ± 0.122	0.929GlyCys: 0.929 ± 0.042	3.647GlyAsp: 3.647 ± 0.079	3.826GlyGlu: 3.826 ± 0.078	3.256GlyPhe: 3.256 ± 0.066	4.783GlyGly: 4.783 ± 0.132	1.216GlyHis: 1.216 ± 0.046	6.786GlyIle: 6.786 ± 0.119	4.953GlyLys: 4.953 ± 0.088	6.138GlyLeu: 6.138 ± 0.129	1.992GlyMet: 1.992 ± 0.054	3.711GlyAsn: 3.711 ± 0.116	1.983GlyPro: 1.983 ± 0.059	1.561GlyGln: 1.561 ± 0.045	2.324GlyArg: 2.324 ± 0.059	4.756GlySer: 4.756 ± 0.089	4.667GlyThr: 4.667 ± 0.143	5.283GlyVal: 5.283 ± 0.111	0.662GlyTrp: 0.662 ± 0.029	2.824GlyTyr: 2.824 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.917HisAla: 0.917 ± 0.043	0.233HisCys: 0.233 ± 0.016	1.045HisAsp: 1.045 ± 0.036	1.276HisGlu: 1.276 ± 0.051	0.64HisPhe: 0.64 ± 0.029	1.399HisGly: 1.399 ± 0.048	0.457HisHis: 0.457 ± 0.025	1.266HisIle: 1.266 ± 0.041	1.016HisLys: 1.016 ± 0.042	1.401HisLeu: 1.401 ± 0.042	0.419HisMet: 0.419 ± 0.025	0.775HisAsn: 0.775 ± 0.032	0.929HisPro: 0.929 ± 0.04	0.439HisGln: 0.439 ± 0.025	0.657HisArg: 0.657 ± 0.032	1.133HisSer: 1.133 ± 0.037	0.844HisThr: 0.844 ± 0.038	1.277HisVal: 1.277 ± 0.042	0.126HisTrp: 0.126 ± 0.014	0.664HisTyr: 0.664 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.414IleAla: 5.414 ± 0.102	0.972IleCys: 0.972 ± 0.036	4.521IleAsp: 4.521 ± 0.073	5.934IleGlu: 5.934 ± 0.12	4.125IlePhe: 4.125 ± 0.105	6.124IleGly: 6.124 ± 0.122	1.513IleHis: 1.513 ± 0.05	8.309IleIle: 8.309 ± 0.152	6.68IleLys: 6.68 ± 0.091	8.529IleLeu: 8.529 ± 0.131	2.326IleMet: 2.326 ± 0.067	4.928IleAsn: 4.928 ± 0.103	3.585IlePro: 3.585 ± 0.082	2.21IleGln: 2.21 ± 0.059	2.785IleArg: 2.785 ± 0.066	6.042IleSer: 6.042 ± 0.103	5.22IleThr: 5.22 ± 0.102	5.896IleVal: 5.896 ± 0.101	0.682IleTrp: 0.682 ± 0.035	3.348IleTyr: 3.348 ± 0.081	0.0IleXaa: 0.0 ± 0.0
Lys	3.792LysAla: 3.792 ± 0.081	0.903LysCys: 0.903 ± 0.05	4.312LysAsp: 4.312 ± 0.102	5.596LysGlu: 5.596 ± 0.12	2.979LysPhe: 2.979 ± 0.07	4.147LysGly: 4.147 ± 0.085	1.252LysHis: 1.252 ± 0.044	7.018LysIle: 7.018 ± 0.117	6.248LysLys: 6.248 ± 0.122	6.552LysLeu: 6.552 ± 0.112	2.159LysMet: 2.159 ± 0.054	5.174LysAsn: 5.174 ± 0.088	2.688LysPro: 2.688 ± 0.067	1.896LysGln: 1.896 ± 0.05	2.779LysArg: 2.779 ± 0.07	4.484LysSer: 4.484 ± 0.09	4.934LysThr: 4.934 ± 0.08	4.39LysVal: 4.39 ± 0.074	0.594LysTrp: 0.594 ± 0.034	2.933LysTyr: 2.933 ± 0.074	0.0LysXaa: 0.0 ± 0.0
Leu	5.512LeuAla: 5.512 ± 0.1	0.915LeuCys: 0.915 ± 0.043	5.139LeuAsp: 5.139 ± 0.096	6.636LeuGlu: 6.636 ± 0.135	3.703LeuPhe: 3.703 ± 0.094	6.184LeuGly: 6.184 ± 0.103	1.291LeuHis: 1.291 ± 0.042	8.322LeuIle: 8.322 ± 0.161	8.349LeuLys: 8.349 ± 0.128	7.959LeuLeu: 7.959 ± 0.146	2.544LeuMet: 2.544 ± 0.064	5.666LeuAsn: 5.666 ± 0.11	3.14LeuPro: 3.14 ± 0.066	2.096LeuGln: 2.096 ± 0.058	3.071LeuArg: 3.071 ± 0.069	5.842LeuSer: 5.842 ± 0.098	5.05LeuThr: 5.05 ± 0.084	6.11LeuVal: 6.11 ± 0.098	0.676LeuTrp: 0.676 ± 0.03	2.768LeuTyr: 2.768 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	2.001MetAla: 2.001 ± 0.055	0.246MetCys: 0.246 ± 0.02	2.044MetAsp: 2.044 ± 0.053	2.261MetGlu: 2.261 ± 0.06	0.964MetPhe: 0.964 ± 0.035	2.28MetGly: 2.28 ± 0.057	0.429MetHis: 0.429 ± 0.027	2.373MetIle: 2.373 ± 0.058	2.244MetLys: 2.244 ± 0.058	2.127MetLeu: 2.127 ± 0.054	0.756MetMet: 0.756 ± 0.036	1.573MetAsn: 1.573 ± 0.044	0.896MetPro: 0.896 ± 0.036	0.606MetGln: 0.606 ± 0.029	0.865MetArg: 0.865 ± 0.033	1.58MetSer: 1.58 ± 0.052	1.285MetThr: 1.285 ± 0.046	2.331MetVal: 2.331 ± 0.059	0.163MetTrp: 0.163 ± 0.015	0.671MetTyr: 0.671 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.014AsnAla: 3.014 ± 0.076	0.647AsnCys: 0.647 ± 0.038	2.584AsnAsp: 2.584 ± 0.063	3.314AsnGlu: 3.314 ± 0.082	2.373AsnPhe: 2.373 ± 0.063	4.143AsnGly: 4.143 ± 0.136	1.037AsnHis: 1.037 ± 0.036	5.121AsnIle: 5.121 ± 0.1	3.894AsnLys: 3.894 ± 0.078	5.147AsnLeu: 5.147 ± 0.1	1.589AsnMet: 1.589 ± 0.05	3.923AsnAsn: 3.923 ± 0.188	2.656AsnPro: 2.656 ± 0.074	1.89AsnGln: 1.89 ± 0.06	1.811AsnArg: 1.811 ± 0.067	4.39AsnSer: 4.39 ± 0.124	3.328AsnThr: 3.328 ± 0.119	3.682AsnVal: 3.682 ± 0.071	0.584AsnTrp: 0.584 ± 0.032	2.392AsnTyr: 2.392 ± 0.069	0.0AsnXaa: 0.0 ± 0.0
Pro	1.941ProAla: 1.941 ± 0.059	0.43ProCys: 0.43 ± 0.027	2.421ProAsp: 2.421 ± 0.061	3.433ProGlu: 3.433 ± 0.088	1.576ProPhe: 1.576 ± 0.048	2.366ProGly: 2.366 ± 0.074	0.676ProHis: 0.676 ± 0.033	2.835ProIle: 2.835 ± 0.06	2.437ProLys: 2.437 ± 0.067	3.235ProLeu: 3.235 ± 0.07	0.953ProMet: 0.953 ± 0.038	1.786ProAsn: 1.786 ± 0.049	1.07ProPro: 1.07 ± 0.038	1.037ProGln: 1.037 ± 0.043	1.118ProArg: 1.118 ± 0.043	2.273ProSer: 2.273 ± 0.056	2.053ProThr: 2.053 ± 0.068	3.077ProVal: 3.077 ± 0.08	0.295ProTrp: 0.295 ± 0.022	1.301ProTyr: 1.301 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	1.442GlnAla: 1.442 ± 0.047	0.228GlnCys: 0.228 ± 0.021	1.285GlnAsp: 1.285 ± 0.05	1.643GlnGlu: 1.643 ± 0.058	0.986GlnPhe: 0.986 ± 0.034	1.513GlnGly: 1.513 ± 0.045	0.437GlnHis: 0.437 ± 0.027	2.373GlnIle: 2.373 ± 0.055	2.047GlnLys: 2.047 ± 0.057	2.233GlnLeu: 2.233 ± 0.065	0.753GlnMet: 0.753 ± 0.03	1.698GlnAsn: 1.698 ± 0.055	0.738GlnPro: 0.738 ± 0.032	0.673GlnGln: 0.673 ± 0.031	1.049GlnArg: 1.049 ± 0.042	1.547GlnSer: 1.547 ± 0.043	1.664GlnThr: 1.664 ± 0.045	1.488GlnVal: 1.488 ± 0.046	0.189GlnTrp: 0.189 ± 0.016	0.887GlnTyr: 0.887 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.082ArgAla: 2.082 ± 0.056	0.428ArgCys: 0.428 ± 0.025	1.9ArgAsp: 1.9 ± 0.057	2.711ArgGlu: 2.711 ± 0.063	1.373ArgPhe: 1.373 ± 0.047	2.216ArgGly: 2.216 ± 0.06	0.456ArgHis: 0.456 ± 0.024	3.102ArgIle: 3.102 ± 0.066	2.736ArgLys: 2.736 ± 0.072	2.706ArgLeu: 2.706 ± 0.066	1.084ArgMet: 1.084 ± 0.038	1.835ArgAsn: 1.835 ± 0.056	0.957ArgPro: 0.957 ± 0.046	0.77ArgGln: 0.77 ± 0.036	1.569ArgArg: 1.569 ± 0.057	2.266ArgSer: 2.266 ± 0.059	1.74ArgThr: 1.74 ± 0.044	2.361ArgVal: 2.361 ± 0.071	0.263ArgTrp: 0.263 ± 0.023	1.276ArgTyr: 1.276 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	3.689SerAla: 3.689 ± 0.077	0.725SerCys: 0.725 ± 0.039	3.344SerAsp: 3.344 ± 0.08	4.252SerGlu: 4.252 ± 0.098	2.789SerPhe: 2.789 ± 0.073	5.066SerGly: 5.066 ± 0.122	1.042SerHis: 1.042 ± 0.039	5.624SerIle: 5.624 ± 0.101	5.083SerLys: 5.083 ± 0.095	5.733SerLeu: 5.733 ± 0.103	1.699SerMet: 1.699 ± 0.049	3.856SerAsn: 3.856 ± 0.122	2.259SerPro: 2.259 ± 0.054	1.815SerGln: 1.815 ± 0.05	2.243SerArg: 2.243 ± 0.056	4.864SerSer: 4.864 ± 0.114	3.996SerThr: 3.996 ± 0.091	4.26SerVal: 4.26 ± 0.094	0.567SerTrp: 0.567 ± 0.028	2.563SerTyr: 2.563 ± 0.07	0.0SerXaa: 0.0 ± 0.0
Thr	4.02ThrAla: 4.02 ± 0.105	0.625ThrCys: 0.625 ± 0.036	3.135ThrAsp: 3.135 ± 0.072	3.365ThrGlu: 3.365 ± 0.068	2.191ThrPhe: 2.191 ± 0.061	4.843ThrGly: 4.843 ± 0.114	0.993ThrHis: 0.993 ± 0.037	5.146ThrIle: 5.146 ± 0.115	3.532ThrLys: 3.532 ± 0.072	5.092ThrLeu: 5.092 ± 0.106	1.389ThrMet: 1.389 ± 0.042	3.17ThrAsn: 3.17 ± 0.108	2.391ThrPro: 2.391 ± 0.074	1.452ThrGln: 1.452 ± 0.051	1.695ThrArg: 1.695 ± 0.051	3.905ThrSer: 3.905 ± 0.108	3.637ThrThr: 3.637 ± 0.109	4.897ThrVal: 4.897 ± 0.125	0.4ThrTrp: 0.4 ± 0.023	1.885ThrTyr: 1.885 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	4.755ValAla: 4.755 ± 0.086	0.833ValCys: 0.833 ± 0.04	4.378ValAsp: 4.378 ± 0.097	5.255ValGlu: 5.255 ± 0.101	2.771ValPhe: 2.771 ± 0.076	5.09ValGly: 5.09 ± 0.097	1.196ValHis: 1.196 ± 0.038	5.541ValIle: 5.541 ± 0.111	5.226ValLys: 5.226 ± 0.096	6.684ValLeu: 6.684 ± 0.106	1.797ValMet: 1.797 ± 0.055	3.823ValAsn: 3.823 ± 0.088	2.755ValPro: 2.755 ± 0.067	1.615ValGln: 1.615 ± 0.054	2.169ValArg: 2.169 ± 0.065	4.758ValSer: 4.758 ± 0.095	4.239ValThr: 4.239 ± 0.132	5.887ValVal: 5.887 ± 0.114	0.524ValTrp: 0.524 ± 0.029	2.459ValTyr: 2.459 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.444TrpAla: 0.444 ± 0.024	0.077TrpCys: 0.077 ± 0.01	0.471TrpAsp: 0.471 ± 0.027	0.437TrpGlu: 0.437 ± 0.026	0.367TrpPhe: 0.367 ± 0.024	0.538TrpGly: 0.538 ± 0.03	0.126TrpHis: 0.126 ± 0.014	0.784TrpIle: 0.784 ± 0.038	0.613TrpLys: 0.613 ± 0.03	0.701TrpLeu: 0.701 ± 0.037	0.245TrpMet: 0.245 ± 0.018	0.616TrpAsn: 0.616 ± 0.031	0.207TrpPro: 0.207 ± 0.018	0.236TrpGln: 0.236 ± 0.017	0.267TrpArg: 0.267 ± 0.021	0.468TrpSer: 0.468 ± 0.029	0.404TrpThr: 0.404 ± 0.026	0.468TrpVal: 0.468 ± 0.026	0.162TrpTrp: 0.162 ± 0.017	0.312TrpTyr: 0.312 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.804TyrAla: 1.804 ± 0.05	0.435TyrCys: 0.435 ± 0.028	1.99TyrAsp: 1.99 ± 0.056	2.107TyrGlu: 2.107 ± 0.073	1.628TyrPhe: 1.628 ± 0.058	2.804TyrGly: 2.804 ± 0.068	0.682TyrHis: 0.682 ± 0.035	2.908TyrIle: 2.908 ± 0.069	2.402TyrLys: 2.402 ± 0.062	3.423TyrLeu: 3.423 ± 0.073	0.954TyrMet: 0.954 ± 0.037	2.399TyrAsn: 2.399 ± 0.085	1.48TyrPro: 1.48 ± 0.047	1.061TyrGln: 1.061 ± 0.037	1.27TyrArg: 1.27 ± 0.042	2.535TyrSer: 2.535 ± 0.071	2.019TyrThr: 2.019 ± 0.085	2.319TyrVal: 2.319 ± 0.06	0.33TyrTrp: 0.33 ± 0.024	1.469TyrTyr: 1.469 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2493 proteins (715694 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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