Clostridiales bacterium oral taxon 876 str. F0540

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales; Clostridiales bacterium oral taxon 876

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2D2Q7|U2D2Q7_9FIRM Acetyltransferase GNAT family OS=Clostridiales bacterium oral taxon 876 str. F0540 OX=1321778 GN=HMPREF1982_03430 PE=4 SV=1
MM1 pKa = 7.77AGRR4 pKa = 11.84PIFNGQVLVNEE15 pKa = 4.68GKK17 pKa = 10.34IIEE20 pKa = 4.46IGDD23 pKa = 4.24NITEE27 pKa = 4.27SEE29 pKa = 4.17DD30 pKa = 4.23CEE32 pKa = 5.6IIDD35 pKa = 3.97ADD37 pKa = 4.3GGFVMPGIIDD47 pKa = 3.63AHH49 pKa = 5.7CHH51 pKa = 4.69IGMWEE56 pKa = 3.82EE57 pKa = 4.04GMGFEE62 pKa = 5.06GSDD65 pKa = 3.59GNEE68 pKa = 3.79ITDD71 pKa = 3.72PATPQLRR78 pKa = 11.84AIDD81 pKa = 4.87AINPRR86 pKa = 11.84DD87 pKa = 3.36TAFIEE92 pKa = 4.87AYY94 pKa = 9.56EE95 pKa = 4.0NGITCVCTGPGSANVIGCIGSLL117 pKa = 3.46

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2D247|U2D247_9FIRM ATP-grasp domain protein OS=Clostridiales bacterium oral taxon 876 str. F0540 OX=1321778 GN=HMPREF1982_03585 PE=4 SV=1
FF1 pKa = 7.3LLSIEE6 pKa = 4.42TLGSLGCSFPFARR19 pKa = 11.84LPVEE23 pKa = 3.92VHH25 pKa = 6.64LGLQFGAPLTRR36 pKa = 11.84SFPFARR42 pKa = 11.84LPVEE46 pKa = 3.98VHH48 pKa = 6.96LGLQPPKK55 pKa = 10.32IILKK59 pKa = 10.36RR60 pKa = 11.84KK61 pKa = 7.25TSSLNLMSFILWLSNPISRR80 pKa = 11.84VLYY83 pKa = 10.34

Molecular weight: 9.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4702

0

4702

1392343

33

2881

296.1

33.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.109 ± 0.04

1.089 ± 0.014

5.423 ± 0.025

7.214 ± 0.041

4.513 ± 0.03

6.334 ± 0.03

1.4 ± 0.014

9.491 ± 0.04

8.713 ± 0.032

9.217 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.014

6.018 ± 0.032

2.889 ± 0.018

2.499 ± 0.019

3.557 ± 0.023

6.551 ± 0.028

4.883 ± 0.024

6.427 ± 0.032

0.79 ± 0.014

4.216 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski