Coriobacteriales bacterium DNF00809
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133Z8W7|A0A133Z8W7_9ACTN Chloride transporter ClC family OS=Coriobacteriales bacterium DNF00809 OX=1588753 GN=HMPREF3190_01076 PE=4 SV=1
MM1 pKa = 7.31 SFSGGADD8 pKa = 3.12 TCVVIVGLDD17 pKa = 3.31 EE18 pKa = 4.55 TGTVDD23 pKa = 6.05 DD24 pKa = 5.49 RR25 pKa = 11.84 DD26 pKa = 3.95 GLNPADD32 pKa = 4.24 GSDD35 pKa = 3.41 VDD37 pKa = 4.1 VCAGNDD43 pKa = 3.55 VVDD46 pKa = 4.17 CTRR49 pKa = 11.84 DD50 pKa = 3.58 GEE52 pKa = 4.29 AHH54 pKa = 6.49 AGGVGDD60 pKa = 3.73 VTGCSAVTGDD70 pKa = 4.37 TLCACDD76 pKa = 3.99 GVLSKK81 pKa = 11.52 GDD83 pKa = 3.28 VWCIIGVSQRR93 pKa = 11.84 EE94 pKa = 4.33 AFDD97 pKa = 3.28 STGRR101 pKa = 11.84 LSEE104 pKa = 4.22 VASSGVFDD112 pKa = 5.85 VIPCSKK118 pKa = 10.34 LLYY121 pKa = 8.66 RR122 pKa = 11.84 QGVIEE127 pKa = 4.05 AA128 pKa = 4.35
Molecular weight: 12.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.503
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.164
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A133YYN5|A0A133YYN5_9ACTN Uncharacterized protein OS=Coriobacteriales bacterium DNF00809 OX=1588753 GN=HMPREF3190_01665 PE=4 SV=1
MM1 pKa = 7.51 CVITRR6 pKa = 11.84 AAARR10 pKa = 11.84 SGTRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 SRR18 pKa = 11.84 FRR20 pKa = 11.84 TRR22 pKa = 11.84 SRR24 pKa = 11.84 ASTRR28 pKa = 11.84 ASNAPRR34 pKa = 11.84 ASQRR38 pKa = 11.84 ITSLL42 pKa = 3.22
Molecular weight: 4.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.945
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1709
0
1709
504769
37
2722
295.4
32.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.169 ± 0.079
1.889 ± 0.038
5.411 ± 0.055
5.555 ± 0.062
3.947 ± 0.048
6.467 ± 0.06
2.72 ± 0.035
5.978 ± 0.065
5.124 ± 0.088
9.25 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.441 ± 0.034
3.92 ± 0.055
4.016 ± 0.056
4.029 ± 0.046
5.055 ± 0.064
6.858 ± 0.057
6.014 ± 0.072
7.164 ± 0.06
0.861 ± 0.019
3.134 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here