Amino acid dipepetide frequency for Gordonia phage CloverMinnie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.842AlaAla: 15.842 ± 0.985
0.762AlaCys: 0.762 ± 0.229
8.175AlaAsp: 8.175 ± 0.72
8.682AlaGlu: 8.682 ± 0.786
2.894AlaPhe: 2.894 ± 0.524
8.175AlaGly: 8.175 ± 0.778
2.386AlaHis: 2.386 ± 0.356
4.519AlaIle: 4.519 ± 0.748
3.91AlaLys: 3.91 ± 0.532
10.815AlaLeu: 10.815 ± 0.753
3.656AlaMet: 3.656 ± 0.519
2.589AlaAsn: 2.589 ± 0.466
6.601AlaPro: 6.601 ± 0.613
4.265AlaGln: 4.265 ± 0.513
7.616AlaArg: 7.616 ± 0.805
7.108AlaSer: 7.108 ± 0.681
7.21AlaThr: 7.21 ± 0.681
10.206AlaVal: 10.206 ± 0.706
2.285AlaTrp: 2.285 ± 0.462
2.133AlaTyr: 2.133 ± 0.406
0.0AlaXaa: 0.0 ± 0.0
Cys
0.965CysAla: 0.965 ± 0.219
0.254CysCys: 0.254 ± 0.137
0.965CysAsp: 0.965 ± 0.236
0.609CysGlu: 0.609 ± 0.241
0.203CysPhe: 0.203 ± 0.121
1.422CysGly: 1.422 ± 0.323
0.254CysHis: 0.254 ± 0.101
0.355CysIle: 0.355 ± 0.129
0.203CysLys: 0.203 ± 0.095
0.355CysLeu: 0.355 ± 0.128
0.102CysMet: 0.102 ± 0.069
0.355CysAsn: 0.355 ± 0.147
0.762CysPro: 0.762 ± 0.258
0.102CysGln: 0.102 ± 0.074
0.762CysArg: 0.762 ± 0.226
0.609CysSer: 0.609 ± 0.191
0.406CysThr: 0.406 ± 0.144
0.812CysVal: 0.812 ± 0.242
0.203CysTrp: 0.203 ± 0.115
0.203CysTyr: 0.203 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
8.632AspAla: 8.632 ± 0.692
0.762AspCys: 0.762 ± 0.27
6.855AspAsp: 6.855 ± 0.996
4.976AspGlu: 4.976 ± 0.5
2.031AspPhe: 2.031 ± 0.261
4.976AspGly: 4.976 ± 0.581
1.219AspHis: 1.219 ± 0.245
1.98AspIle: 1.98 ± 0.341
2.386AspLys: 2.386 ± 0.427
6.702AspLeu: 6.702 ± 0.673
1.777AspMet: 1.777 ± 0.294
1.726AspAsn: 1.726 ± 0.289
4.671AspPro: 4.671 ± 0.518
2.285AspGln: 2.285 ± 0.257
5.027AspArg: 5.027 ± 0.43
3.859AspSer: 3.859 ± 0.428
4.57AspThr: 4.57 ± 0.427
4.976AspVal: 4.976 ± 0.465
1.269AspTrp: 1.269 ± 0.324
1.98AspTyr: 1.98 ± 0.377
0.0AspXaa: 0.0 ± 0.0
Glu
8.124GluAla: 8.124 ± 0.779
0.762GluCys: 0.762 ± 0.246
4.214GluAsp: 4.214 ± 0.49
3.503GluGlu: 3.503 ± 0.51
1.371GluPhe: 1.371 ± 0.228
4.62GluGly: 4.62 ± 0.441
1.168GluHis: 1.168 ± 0.257
4.468GluIle: 4.468 ± 0.416
1.625GluLys: 1.625 ± 0.275
5.89GluLeu: 5.89 ± 0.694
0.965GluMet: 0.965 ± 0.208
1.015GluAsn: 1.015 ± 0.19
3.453GluPro: 3.453 ± 0.491
2.894GluGln: 2.894 ± 0.413
3.757GluArg: 3.757 ± 0.329
2.589GluSer: 2.589 ± 0.342
4.367GluThr: 4.367 ± 0.591
4.163GluVal: 4.163 ± 0.457
1.32GluTrp: 1.32 ± 0.229
1.32GluTyr: 1.32 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
3.503PheAla: 3.503 ± 0.367
0.203PheCys: 0.203 ± 0.108
2.589PheAsp: 2.589 ± 0.419
1.371PheGlu: 1.371 ± 0.275
0.559PhePhe: 0.559 ± 0.239
2.183PheGly: 2.183 ± 0.293
0.406PheHis: 0.406 ± 0.128
1.015PheIle: 1.015 ± 0.29
0.559PheLys: 0.559 ± 0.152
1.676PheLeu: 1.676 ± 0.364
0.762PheMet: 0.762 ± 0.192
0.406PheAsn: 0.406 ± 0.138
1.015PhePro: 1.015 ± 0.187
0.609PheGln: 0.609 ± 0.219
1.117PheArg: 1.117 ± 0.252
1.371PheSer: 1.371 ± 0.235
2.336PheThr: 2.336 ± 0.267
1.625PheVal: 1.625 ± 0.309
0.559PheTrp: 0.559 ± 0.191
0.305PheTyr: 0.305 ± 0.125
0.0PheXaa: 0.0 ± 0.0
Gly
6.804GlyAla: 6.804 ± 0.53
0.812GlyCys: 0.812 ± 0.235
5.331GlyAsp: 5.331 ± 0.473
6.042GlyGlu: 6.042 ± 0.478
2.183GlyPhe: 2.183 ± 0.406
7.464GlyGly: 7.464 ± 1.154
1.828GlyHis: 1.828 ± 0.366
2.945GlyIle: 2.945 ± 0.57
2.183GlyLys: 2.183 ± 0.371
8.225GlyLeu: 8.225 ± 0.697
1.726GlyMet: 1.726 ± 0.266
2.64GlyAsn: 2.64 ± 0.373
3.808GlyPro: 3.808 ± 0.455
3.046GlyGln: 3.046 ± 0.449
6.448GlyArg: 6.448 ± 0.701
4.976GlySer: 4.976 ± 0.525
5.636GlyThr: 5.636 ± 0.538
6.651GlyVal: 6.651 ± 0.508
2.336GlyTrp: 2.336 ± 0.384
2.843GlyTyr: 2.843 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
1.929HisAla: 1.929 ± 0.324
0.102HisCys: 0.102 ± 0.065
1.371HisAsp: 1.371 ± 0.252
1.219HisGlu: 1.219 ± 0.31
0.609HisPhe: 0.609 ± 0.166
1.168HisGly: 1.168 ± 0.243
0.711HisHis: 0.711 ± 0.163
0.609HisIle: 0.609 ± 0.176
0.305HisLys: 0.305 ± 0.139
1.879HisLeu: 1.879 ± 0.369
0.203HisMet: 0.203 ± 0.12
0.305HisAsn: 0.305 ± 0.104
1.422HisPro: 1.422 ± 0.261
0.762HisGln: 0.762 ± 0.188
1.574HisArg: 1.574 ± 0.322
1.066HisSer: 1.066 ± 0.225
1.422HisThr: 1.422 ± 0.262
0.965HisVal: 0.965 ± 0.263
0.406HisTrp: 0.406 ± 0.108
0.254HisTyr: 0.254 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
6.093IleAla: 6.093 ± 0.701
0.406IleCys: 0.406 ± 0.158
4.011IleAsp: 4.011 ± 0.431
4.316IleGlu: 4.316 ± 0.527
0.812IlePhe: 0.812 ± 0.189
4.113IleGly: 4.113 ± 0.716
0.609IleHis: 0.609 ± 0.2
1.98IleIle: 1.98 ± 0.327
1.32IleLys: 1.32 ± 0.468
1.726IleLeu: 1.726 ± 0.274
0.762IleMet: 0.762 ± 0.242
1.422IleAsn: 1.422 ± 0.3
1.625IlePro: 1.625 ± 0.405
1.371IleGln: 1.371 ± 0.33
2.285IleArg: 2.285 ± 0.323
3.453IleSer: 3.453 ± 0.481
3.097IleThr: 3.097 ± 0.461
4.265IleVal: 4.265 ± 0.477
0.711IleTrp: 0.711 ± 0.211
0.711IleTyr: 0.711 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
3.808LysAla: 3.808 ± 0.524
0.051LysCys: 0.051 ± 0.059
1.879LysAsp: 1.879 ± 0.291
0.762LysGlu: 0.762 ± 0.207
0.559LysPhe: 0.559 ± 0.278
2.488LysGly: 2.488 ± 0.332
0.305LysHis: 0.305 ± 0.134
2.183LysIle: 2.183 ± 0.374
1.117LysLys: 1.117 ± 0.41
2.285LysLeu: 2.285 ± 0.323
0.305LysMet: 0.305 ± 0.103
0.812LysAsn: 0.812 ± 0.16
1.472LysPro: 1.472 ± 0.452
1.168LysGln: 1.168 ± 0.25
1.422LysArg: 1.422 ± 0.333
1.32LysSer: 1.32 ± 0.276
1.269LysThr: 1.269 ± 0.261
2.894LysVal: 2.894 ± 0.355
0.051LysTrp: 0.051 ± 0.05
0.863LysTyr: 0.863 ± 0.178
0.0LysXaa: 0.0 ± 0.0
Leu
10.307LeuAla: 10.307 ± 0.712
0.914LeuCys: 0.914 ± 0.262
7.565LeuAsp: 7.565 ± 0.632
3.503LeuGlu: 3.503 ± 0.354
2.234LeuPhe: 2.234 ± 0.41
7.565LeuGly: 7.565 ± 0.681
1.726LeuHis: 1.726 ± 0.325
4.011LeuIle: 4.011 ± 0.5
2.082LeuLys: 2.082 ± 0.292
5.839LeuLeu: 5.839 ± 0.504
2.082LeuMet: 2.082 ± 0.384
2.843LeuAsn: 2.843 ± 0.35
4.671LeuPro: 4.671 ± 0.635
2.843LeuGln: 2.843 ± 0.492
5.128LeuArg: 5.128 ± 0.572
3.96LeuSer: 3.96 ± 0.411
5.991LeuThr: 5.991 ± 0.463
5.382LeuVal: 5.382 ± 0.493
1.625LeuTrp: 1.625 ± 0.27
1.929LeuTyr: 1.929 ± 0.388
0.0LeuXaa: 0.0 ± 0.0
Met
2.945MetAla: 2.945 ± 0.4
0.305MetCys: 0.305 ± 0.142
1.168MetAsp: 1.168 ± 0.255
1.168MetGlu: 1.168 ± 0.268
0.559MetPhe: 0.559 ± 0.19
1.879MetGly: 1.879 ± 0.274
0.355MetHis: 0.355 ± 0.124
1.219MetIle: 1.219 ± 0.25
0.914MetLys: 0.914 ± 0.225
1.676MetLeu: 1.676 ± 0.308
0.102MetMet: 0.102 ± 0.096
0.457MetAsn: 0.457 ± 0.14
1.726MetPro: 1.726 ± 0.283
0.762MetGln: 0.762 ± 0.197
1.472MetArg: 1.472 ± 0.294
1.879MetSer: 1.879 ± 0.261
1.98MetThr: 1.98 ± 0.319
1.676MetVal: 1.676 ± 0.248
0.406MetTrp: 0.406 ± 0.148
0.305MetTyr: 0.305 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.402AsnAla: 3.402 ± 0.462
0.457AsnCys: 0.457 ± 0.141
1.777AsnAsp: 1.777 ± 0.281
0.914AsnGlu: 0.914 ± 0.196
0.355AsnPhe: 0.355 ± 0.133
3.402AsnGly: 3.402 ± 0.62
0.457AsnHis: 0.457 ± 0.161
0.812AsnIle: 0.812 ± 0.188
0.711AsnLys: 0.711 ± 0.17
2.336AsnLeu: 2.336 ± 0.333
0.355AsnMet: 0.355 ± 0.135
1.32AsnAsn: 1.32 ± 0.463
1.32AsnPro: 1.32 ± 0.257
1.066AsnGln: 1.066 ± 0.276
1.371AsnArg: 1.371 ± 0.268
1.828AsnSer: 1.828 ± 0.309
2.285AsnThr: 2.285 ± 0.38
2.437AsnVal: 2.437 ± 0.609
0.355AsnTrp: 0.355 ± 0.123
0.812AsnTyr: 0.812 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
5.687ProAla: 5.687 ± 0.709
0.508ProCys: 0.508 ± 0.176
4.011ProAsp: 4.011 ± 0.461
4.62ProGlu: 4.62 ± 0.594
1.625ProPhe: 1.625 ± 0.26
5.128ProGly: 5.128 ± 0.545
0.66ProHis: 0.66 ± 0.198
2.488ProIle: 2.488 ± 0.384
1.066ProLys: 1.066 ± 0.2
3.148ProLeu: 3.148 ± 0.377
1.574ProMet: 1.574 ± 0.258
1.371ProAsn: 1.371 ± 0.225
2.691ProPro: 2.691 ± 0.388
1.929ProGln: 1.929 ± 0.353
3.503ProArg: 3.503 ± 0.409
3.707ProSer: 3.707 ± 0.589
3.554ProThr: 3.554 ± 0.364
5.534ProVal: 5.534 ± 0.481
1.168ProTrp: 1.168 ± 0.214
1.625ProTyr: 1.625 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
4.976GlnAla: 4.976 ± 0.463
0.203GlnCys: 0.203 ± 0.092
1.32GlnAsp: 1.32 ± 0.229
1.371GlnGlu: 1.371 ± 0.364
0.812GlnPhe: 0.812 ± 0.175
2.945GlnGly: 2.945 ± 0.35
0.406GlnHis: 0.406 ± 0.159
2.082GlnIle: 2.082 ± 0.274
0.812GlnLys: 0.812 ± 0.228
3.859GlnLeu: 3.859 ± 0.504
0.914GlnMet: 0.914 ± 0.194
1.574GlnAsn: 1.574 ± 0.594
1.929GlnPro: 1.929 ± 0.311
1.015GlnGln: 1.015 ± 0.23
2.488GlnArg: 2.488 ± 0.35
1.219GlnSer: 1.219 ± 0.304
2.386GlnThr: 2.386 ± 0.37
2.336GlnVal: 2.336 ± 0.303
0.914GlnTrp: 0.914 ± 0.242
0.812GlnTyr: 0.812 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
8.479ArgAla: 8.479 ± 0.654
0.559ArgCys: 0.559 ± 0.165
4.011ArgAsp: 4.011 ± 0.499
4.316ArgGlu: 4.316 ± 0.484
1.625ArgPhe: 1.625 ± 0.347
4.163ArgGly: 4.163 ± 0.421
1.625ArgHis: 1.625 ± 0.341
2.285ArgIle: 2.285 ± 0.315
2.133ArgLys: 2.133 ± 0.343
5.941ArgLeu: 5.941 ± 0.596
1.117ArgMet: 1.117 ± 0.241
1.726ArgAsn: 1.726 ± 0.334
3.656ArgPro: 3.656 ± 0.564
2.539ArgGln: 2.539 ± 0.296
5.687ArgArg: 5.687 ± 0.717
4.265ArgSer: 4.265 ± 0.511
4.011ArgThr: 4.011 ± 0.432
4.824ArgVal: 4.824 ± 0.502
1.726ArgTrp: 1.726 ± 0.369
2.082ArgTyr: 2.082 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
6.956SerAla: 6.956 ± 0.602
0.914SerCys: 0.914 ± 0.263
4.062SerAsp: 4.062 ± 0.451
2.539SerGlu: 2.539 ± 0.45
0.914SerPhe: 0.914 ± 0.26
6.144SerGly: 6.144 ± 0.576
0.812SerHis: 0.812 ± 0.212
3.402SerIle: 3.402 ± 0.548
1.371SerLys: 1.371 ± 0.276
3.859SerLeu: 3.859 ± 0.553
1.98SerMet: 1.98 ± 0.309
1.219SerAsn: 1.219 ± 0.277
3.707SerPro: 3.707 ± 0.428
2.285SerGln: 2.285 ± 0.349
3.148SerArg: 3.148 ± 0.448
3.25SerSer: 3.25 ± 0.508
3.808SerThr: 3.808 ± 0.352
4.367SerVal: 4.367 ± 0.463
1.676SerTrp: 1.676 ± 0.291
1.472SerTyr: 1.472 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
8.022ThrAla: 8.022 ± 0.633
0.711ThrCys: 0.711 ± 0.307
4.214ThrAsp: 4.214 ± 0.504
3.757ThrGlu: 3.757 ± 0.476
1.726ThrPhe: 1.726 ± 0.305
5.839ThrGly: 5.839 ± 0.411
1.015ThrHis: 1.015 ± 0.205
3.148ThrIle: 3.148 ± 0.439
1.676ThrLys: 1.676 ± 0.324
5.534ThrLeu: 5.534 ± 0.581
1.828ThrMet: 1.828 ± 0.325
2.285ThrAsn: 2.285 ± 0.319
4.925ThrPro: 4.925 ± 0.603
1.32ThrGln: 1.32 ± 0.27
4.874ThrArg: 4.874 ± 0.511
4.163ThrSer: 4.163 ± 0.482
3.25ThrThr: 3.25 ± 0.462
5.128ThrVal: 5.128 ± 0.528
1.422ThrTrp: 1.422 ± 0.261
1.726ThrTyr: 1.726 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
8.479ValAla: 8.479 ± 0.585
0.66ValCys: 0.66 ± 0.188
5.839ValAsp: 5.839 ± 0.548
5.737ValGlu: 5.737 ± 0.492
1.828ValPhe: 1.828 ± 0.344
6.753ValGly: 6.753 ± 0.558
1.422ValHis: 1.422 ± 0.269
4.265ValIle: 4.265 ± 0.925
1.828ValLys: 1.828 ± 0.279
6.398ValLeu: 6.398 ± 0.582
1.422ValMet: 1.422 ± 0.238
1.98ValAsn: 1.98 ± 0.312
3.859ValPro: 3.859 ± 0.282
2.133ValGln: 2.133 ± 0.31
5.737ValArg: 5.737 ± 0.646
3.757ValSer: 3.757 ± 0.482
5.941ValThr: 5.941 ± 0.7
5.991ValVal: 5.991 ± 0.609
1.879ValTrp: 1.879 ± 0.348
1.828ValTyr: 1.828 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
2.386TrpAla: 2.386 ± 0.369
0.305TrpCys: 0.305 ± 0.122
1.472TrpAsp: 1.472 ± 0.261
1.015TrpGlu: 1.015 ± 0.207
0.66TrpPhe: 0.66 ± 0.231
1.066TrpGly: 1.066 ± 0.297
0.508TrpHis: 0.508 ± 0.17
0.559TrpIle: 0.559 ± 0.142
0.457TrpLys: 0.457 ± 0.129
1.98TrpLeu: 1.98 ± 0.255
0.508TrpMet: 0.508 ± 0.195
1.015TrpAsn: 1.015 ± 0.391
0.863TrpPro: 0.863 ± 0.217
0.965TrpGln: 0.965 ± 0.292
1.32TrpArg: 1.32 ± 0.222
1.828TrpSer: 1.828 ± 0.313
1.625TrpThr: 1.625 ± 0.322
1.574TrpVal: 1.574 ± 0.293
0.406TrpTrp: 0.406 ± 0.159
0.66TrpTyr: 0.66 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.488TyrAla: 2.488 ± 0.388
0.254TyrCys: 0.254 ± 0.156
1.828TyrAsp: 1.828 ± 0.295
1.168TyrGlu: 1.168 ± 0.287
0.609TyrPhe: 0.609 ± 0.161
2.488TyrGly: 2.488 ± 0.308
0.406TyrHis: 0.406 ± 0.108
0.762TyrIle: 0.762 ± 0.186
0.355TyrLys: 0.355 ± 0.147
2.082TyrLeu: 2.082 ± 0.384
0.762TyrMet: 0.762 ± 0.167
0.711TyrAsn: 0.711 ± 0.172
1.422TyrPro: 1.422 ± 0.29
1.015TyrGln: 1.015 ± 0.258
2.031TyrArg: 2.031 ± 0.35
1.676TyrSer: 1.676 ± 0.325
1.422TyrThr: 1.422 ± 0.282
1.929TyrVal: 1.929 ± 0.304
0.457TyrTrp: 0.457 ± 0.139
0.66TyrTyr: 0.66 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (19696 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski