Mycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma conjunctivae

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 695 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5J692|C5J692_MYCCR Uncharacterized protein OS=Mycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147) OX=572263 GN=MCJ_002930 PE=4 SV=1
MM1 pKa = 7.47LSKK4 pKa = 10.78VHH6 pKa = 6.23IKK8 pKa = 10.49KK9 pKa = 9.63IDD11 pKa = 3.4EE12 pKa = 4.19LQYY15 pKa = 10.72SEE17 pKa = 5.64EE18 pKa = 4.13EE19 pKa = 3.85FEE21 pKa = 5.27QIYY24 pKa = 9.57KK25 pKa = 9.12QQIEE29 pKa = 4.36EE30 pKa = 3.99ATPIVKK36 pKa = 10.18DD37 pKa = 4.08LSTTEE42 pKa = 3.65QFEE45 pKa = 4.56RR46 pKa = 11.84ISRR49 pKa = 11.84ILFMSDD55 pKa = 3.12SEE57 pKa = 4.61DD58 pKa = 3.74NEE60 pKa = 4.12PVEE63 pKa = 6.02CPTCDD68 pKa = 3.3LEE70 pKa = 5.27VVLEE74 pKa = 4.2EE75 pKa = 4.06QSCPCEE81 pKa = 4.17LIEE84 pKa = 4.92IQQEE88 pKa = 4.13PKK90 pKa = 9.95IEE92 pKa = 4.01EE93 pKa = 4.29SQEE96 pKa = 3.83LASEE100 pKa = 4.25MIEE103 pKa = 3.99EE104 pKa = 4.51VSCSIDD110 pKa = 3.32EE111 pKa = 4.17EE112 pKa = 4.87KK113 pKa = 10.76EE114 pKa = 3.67IEE116 pKa = 4.18EE117 pKa = 4.21VEE119 pKa = 4.16KK120 pKa = 11.3VEE122 pKa = 4.08EE123 pKa = 4.34VEE125 pKa = 5.1SEE127 pKa = 4.07EE128 pKa = 5.07LGCTNCQNPPVCNVDD143 pKa = 4.5PIAVCDD149 pKa = 3.7SCEE152 pKa = 4.33VEE154 pKa = 3.91VDD156 pKa = 3.46EE157 pKa = 6.83ANDD160 pKa = 3.55QSCQCNYY167 pKa = 10.48CC168 pKa = 3.6

Molecular weight: 19.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5J5W1|C5J5W1_MYCCR Arginine--tRNA ligase OS=Mycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147) OX=572263 GN=argS PE=3 SV=1
MM1 pKa = 7.73ANPHH5 pKa = 5.4QIYY8 pKa = 10.23RR9 pKa = 11.84RR10 pKa = 11.84DD11 pKa = 3.61ATWRR15 pKa = 11.84KK16 pKa = 8.94HH17 pKa = 4.37VLRR20 pKa = 11.84SQATALLMHH29 pKa = 6.59GSITTTLARR38 pKa = 11.84AKK40 pKa = 9.57EE41 pKa = 3.96LRR43 pKa = 11.84KK44 pKa = 9.75HH45 pKa = 4.82VEE47 pKa = 3.66KK48 pKa = 11.02LITKK52 pKa = 8.79GKK54 pKa = 10.17KK55 pKa = 8.67DD56 pKa = 3.22TLAARR61 pKa = 11.84RR62 pKa = 11.84NVIKK66 pKa = 10.09FIRR69 pKa = 11.84HH70 pKa = 5.3EE71 pKa = 4.1KK72 pKa = 7.98TKK74 pKa = 10.36EE75 pKa = 3.79GIYY78 pKa = 10.48IMSHH82 pKa = 6.38LFNNIAPKK90 pKa = 10.68YY91 pKa = 8.14KK92 pKa = 10.23DD93 pKa = 3.34RR94 pKa = 11.84NGGYY98 pKa = 7.82TRR100 pKa = 11.84IIKK103 pKa = 10.4LPARR107 pKa = 11.84QGDD110 pKa = 4.0SSKK113 pKa = 10.38MAIIEE118 pKa = 4.16LVV120 pKa = 3.09

Molecular weight: 13.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

695

0

695

256286

34

3915

368.8

42.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.524 ± 0.089

0.322 ± 0.018

5.462 ± 0.08

5.953 ± 0.079

6.156 ± 0.084

4.638 ± 0.083

1.388 ± 0.038

9.736 ± 0.112

9.883 ± 0.107

9.368 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.297 ± 0.035

7.669 ± 0.098

2.832 ± 0.048

4.539 ± 0.062

2.831 ± 0.055

7.302 ± 0.085

5.104 ± 0.099

5.3 ± 0.066

0.881 ± 0.031

3.814 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski