Kiloniella litopenaei
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3735 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M2R471|A0A0M2R471_9PROT Bifunctional protein GlmU OS=Kiloniella litopenaei OX=1549748 GN=glmU PE=3 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.01 KK3 pKa = 9.96 ILMASTALAASLSYY17 pKa = 10.49 AASASAEE24 pKa = 4.02 DD25 pKa = 4.39 TKK27 pKa = 11.07 IKK29 pKa = 9.75 WSAYY33 pKa = 7.57 TDD35 pKa = 4.02 FNVSSTSVDD44 pKa = 3.28 GNVPGGDD51 pKa = 3.65 TNNGLDD57 pKa = 3.6 FNTNSEE63 pKa = 3.76 IHH65 pKa = 6.5 LNASQTADD73 pKa = 3.0 NGLTYY78 pKa = 10.69 GLHH81 pKa = 5.96 IQLEE85 pKa = 4.34 ADD87 pKa = 3.25 QGNTNNTDD95 pKa = 3.23 EE96 pKa = 4.04 NHH98 pKa = 6.02 IFIEE102 pKa = 4.53 GDD104 pKa = 3.28 FGRR107 pKa = 11.84 VEE109 pKa = 5.76 LGDD112 pKa = 3.44 QDD114 pKa = 4.12 SAGDD118 pKa = 3.34 RR119 pKa = 11.84 MMVSGSSVGFQYY131 pKa = 11.68 GMFGNYY137 pKa = 8.64 TGGLSALEE145 pKa = 4.34 DD146 pKa = 3.67 EE147 pKa = 5.24 GNLGRR152 pKa = 11.84 TAADD156 pKa = 3.98 FSDD159 pKa = 4.1 SSDD162 pKa = 3.4 SSKK165 pKa = 9.26 ITYY168 pKa = 7.58 FTPTFNGFKK177 pKa = 10.21 AGLSFAPDD185 pKa = 3.42 SDD187 pKa = 3.74 SGQTVSGNEE196 pKa = 3.68 TDD198 pKa = 4.27 FNNHH202 pKa = 5.33 FDD204 pKa = 3.49 AALNYY209 pKa = 10.63 SGTFNDD215 pKa = 3.98 FTVDD219 pKa = 3.16 AALIGATHH227 pKa = 6.92 EE228 pKa = 4.47 VTSQGSSDD236 pKa = 3.37 DD237 pKa = 3.11 TYY239 pKa = 9.67 YY240 pKa = 11.45 AVGGGVMLGYY250 pKa = 10.81 AGFKK254 pKa = 10.02 AALGVLHH261 pKa = 7.74 EE262 pKa = 4.45 DD263 pKa = 3.43 TKK265 pKa = 11.03 DD266 pKa = 3.09 TSEE269 pKa = 4.34 INTVDD274 pKa = 2.9 TGLSYY279 pKa = 11.3 GIGAWSLSVGAAWSEE294 pKa = 3.62 FDD296 pKa = 4.1 QDD298 pKa = 3.34 NGVEE302 pKa = 4.21 NEE304 pKa = 4.35 SIAYY308 pKa = 8.59 SAGVGYY314 pKa = 10.18 EE315 pKa = 3.88 IAPGLTTWAGATIGDD330 pKa = 4.05 YY331 pKa = 11.03 EE332 pKa = 4.78 GGVEE336 pKa = 5.42 DD337 pKa = 4.15 FTNFQTGISVAFF349 pKa = 4.0
Molecular weight: 36.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 0.947
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A0M2R3M3|A0A0M2R3M3_9PROT Peptide transporter OS=Kiloniella litopenaei OX=1549748 GN=WH95_12990 PE=3 SV=1
MM1 pKa = 7.61 AKK3 pKa = 9.99 SFSSTLSVLFSLRR16 pKa = 11.84 TVPVLTLMVFLLPVLAGLFSTIFPAFGYY44 pKa = 10.26 LPAIGQVSFTTAAWTQLFAHH64 pKa = 7.14 PALPNALLTSVSSGIIATLLSLILSLTFVAAWQGTNSFKK103 pKa = 10.81 RR104 pKa = 11.84 ARR106 pKa = 11.84 QLLAPILAIPHH117 pKa = 5.66 SALAIGLAFLIAPSGWITRR136 pKa = 11.84 TISPWATGWEE146 pKa = 4.33 RR147 pKa = 11.84 PPDD150 pKa = 3.09 IAVIHH155 pKa = 6.43 DD156 pKa = 3.58 EE157 pKa = 4.29 WGLSLILGLMIKK169 pKa = 7.63 EE170 pKa = 4.41 TPYY173 pKa = 10.95 LLLMIVAALDD183 pKa = 3.34 QSQAKK188 pKa = 9.14 QRR190 pKa = 11.84 LKK192 pKa = 10.5 LAKK195 pKa = 8.92 TLGYY199 pKa = 9.99 HH200 pKa = 6.61 PVSAWIKK207 pKa = 9.2 GVLPVIYY214 pKa = 9.83 KK215 pKa = 9.38 QIRR218 pKa = 11.84 LPIYY222 pKa = 10.17 AVLAFSLSVVDD233 pKa = 3.83 IALILGPTTPPTLSILVLRR252 pKa = 11.84 WFNDD256 pKa = 2.93 PDD258 pKa = 3.37 LSYY261 pKa = 10.33 RR262 pKa = 11.84 TIAAAGALLQLGVVVGVIFLWWATEE287 pKa = 4.0 KK288 pKa = 10.82 AVSRR292 pKa = 11.84 LTRR295 pKa = 11.84 RR296 pKa = 11.84 WLANGKK302 pKa = 9.8 RR303 pKa = 11.84 SFAEE307 pKa = 3.92 TPVRR311 pKa = 11.84 IFSRR315 pKa = 11.84 ISLNSIFVLFFGSLLVIVFWSFTRR339 pKa = 11.84 RR340 pKa = 11.84 WQYY343 pKa = 11.33 PDD345 pKa = 4.08 FLPSVWSIQSWDD357 pKa = 3.47 RR358 pKa = 11.84 NINNLLWSSSATLIIGLISSLIAVILVIACLEE390 pKa = 3.94 NEE392 pKa = 3.92 KK393 pKa = 10.38 RR394 pKa = 11.84 RR395 pKa = 11.84 KK396 pKa = 9.7 KK397 pKa = 10.58 SAGTGALWLVYY408 pKa = 9.87 LPLMIPQVSFLFGVQVLTIKK428 pKa = 10.84 AGIDD432 pKa = 3.58 ATWLAMIWGHH442 pKa = 6.54 LLFVLPYY449 pKa = 10.27 VFLSLTDD456 pKa = 4.04 PYY458 pKa = 10.45 RR459 pKa = 11.84 QHH461 pKa = 7.49 DD462 pKa = 3.6 DD463 pKa = 3.53 RR464 pKa = 11.84 YY465 pKa = 9.82 IKK467 pKa = 10.5 VALSMGVSPTRR478 pKa = 11.84 AFWRR482 pKa = 11.84 IRR484 pKa = 11.84 LPMLLRR490 pKa = 11.84 PLLFALAIGFAVSVAQYY507 pKa = 10.99 LPTLFIGGGRR517 pKa = 11.84 FSTLTTEE524 pKa = 4.4 AVNLSASGNRR534 pKa = 11.84 RR535 pKa = 11.84 IIAVYY540 pKa = 10.74 ALLQALLPLLCFATALALPNVIFRR564 pKa = 11.84 NRR566 pKa = 11.84 RR567 pKa = 11.84 TLRR570 pKa = 11.84 EE571 pKa = 3.87 HH572 pKa = 6.76
Molecular weight: 63.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.94
IPC_protein 10.833
Toseland 10.76
ProMoST 10.555
Dawson 10.877
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.038
Grimsley 10.935
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.628
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.804
Patrickios 10.716
IPC_peptide 11.008
IPC2_peptide 9.648
IPC2.peptide.svr19 8.342
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3735
0
3735
1159452
41
3542
310.4
34.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.074 ± 0.042
0.965 ± 0.013
6.046 ± 0.049
6.403 ± 0.043
4.052 ± 0.027
7.721 ± 0.047
2.047 ± 0.018
6.422 ± 0.034
5.214 ± 0.043
10.108 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.021
3.785 ± 0.029
4.343 ± 0.025
3.537 ± 0.028
5.071 ± 0.039
6.412 ± 0.031
5.413 ± 0.032
6.973 ± 0.032
1.216 ± 0.018
2.687 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here