Ligilactobacillus acidipiscis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2127 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2KKY0|A0A0R2KKY0_9LACO Phage protein OS=Ligilactobacillus acidipiscis OX=89059 GN=IV43_GL000048 PE=4 SV=1
MM1 pKa = 7.7 DD2 pKa = 4.56 NEE4 pKa = 4.01 MTFYY8 pKa = 10.64 QFLMTLRR15 pKa = 11.84 DD16 pKa = 3.53 PDD18 pKa = 4.86 DD19 pKa = 4.47 YY20 pKa = 12.08 DD21 pKa = 4.13 EE22 pKa = 4.85 VVQFANNAFYY32 pKa = 10.75 DD33 pKa = 3.55 QSFPKK38 pKa = 10.33 QQTNYY43 pKa = 9.96 QKK45 pKa = 10.8 LSQYY49 pKa = 11.5 LEE51 pKa = 4.04 MNGNYY56 pKa = 9.42 LPSMTIFDD64 pKa = 5.07 DD65 pKa = 3.65 AWQQYY70 pKa = 8.45 LEE72 pKa = 4.74 KK73 pKa = 10.46 IQQ75 pKa = 3.45
Molecular weight: 9.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.401
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A0R2KAZ3|A0A0R2KAZ3_9LACO NLPA lipoprotein OS=Ligilactobacillus acidipiscis OX=89059 GN=IV43_GL001231 PE=3 SV=1
MM1 pKa = 7.09 EE2 pKa = 5.33 RR3 pKa = 11.84 CRR5 pKa = 11.84 FCKK8 pKa = 8.82 STKK11 pKa = 8.56 VVKK14 pKa = 10.28 NGKK17 pKa = 9.56 RR18 pKa = 11.84 PTKK21 pKa = 10.46 LKK23 pKa = 10.76 LPSLSEE29 pKa = 3.67 QPLRR33 pKa = 11.84 FRR35 pKa = 11.84 LYY37 pKa = 9.13 KK38 pKa = 9.78 QRR40 pKa = 11.84 YY41 pKa = 5.88 FCRR44 pKa = 11.84 EE45 pKa = 3.63 CHH47 pKa = 5.28 RR48 pKa = 11.84 TFSAKK53 pKa = 9.52 TEE55 pKa = 3.74 LTQRR59 pKa = 11.84 HH60 pKa = 4.93 RR61 pKa = 11.84 SITRR65 pKa = 11.84 RR66 pKa = 11.84 ARR68 pKa = 11.84 QVIHH72 pKa = 6.64 SLAKK76 pKa = 8.68 EE77 pKa = 3.88 TSPVKK82 pKa = 10.75 NIAKK86 pKa = 9.71 IVGVSASSVQRR97 pKa = 11.84 ILYY100 pKa = 9.82 ANKK103 pKa = 8.27 TANVTPHH110 pKa = 7.13 GLPKK114 pKa = 10.27 ALCFAEE120 pKa = 4.35 FRR122 pKa = 11.84 STRR125 pKa = 11.84 GRR127 pKa = 11.84 FSFICIDD134 pKa = 4.43 AKK136 pKa = 10.3 THH138 pKa = 6.11 DD139 pKa = 4.05 CSYY142 pKa = 11.72 SKK144 pKa = 10.26 IACLRR149 pKa = 11.84 RR150 pKa = 11.84 SKK152 pKa = 10.99 NIFSIITFF160 pKa = 4.1
Molecular weight: 18.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.823
IPC_protein 10.599
Toseland 10.994
ProMoST 10.701
Dawson 11.052
Bjellqvist 10.774
Wikipedia 11.257
Rodwell 11.33
Grimsley 11.082
Solomon 11.213
Lehninger 11.184
Nozaki 10.994
DTASelect 10.76
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 11.052
IPC_peptide 11.213
IPC2_peptide 9.984
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2127
0
2127
615153
50
1601
289.2
32.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.527 ± 0.064
0.58 ± 0.015
5.835 ± 0.049
5.996 ± 0.057
4.364 ± 0.045
6.628 ± 0.052
2.042 ± 0.021
6.883 ± 0.054
7.27 ± 0.064
9.807 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.027
4.882 ± 0.037
3.477 ± 0.025
4.783 ± 0.052
3.916 ± 0.039
6.168 ± 0.043
5.651 ± 0.037
6.967 ± 0.043
0.945 ± 0.018
3.536 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here