Amino acid dipepetide frequency for Ligilactobacillus acidipiscis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.86AlaAla: 6.86 ± 0.137
0.442AlaCys: 0.442 ± 0.026
4.765AlaAsp: 4.765 ± 0.102
4.375AlaGlu: 4.375 ± 0.098
3.037AlaPhe: 3.037 ± 0.079
5.742AlaGly: 5.742 ± 0.109
1.435AlaHis: 1.435 ± 0.046
4.953AlaIle: 4.953 ± 0.086
5.504AlaLys: 5.504 ± 0.102
7.4AlaLeu: 7.4 ± 0.114
1.878AlaMet: 1.878 ± 0.051
3.367AlaAsn: 3.367 ± 0.089
2.217AlaPro: 2.217 ± 0.062
3.841AlaGln: 3.841 ± 0.091
2.97AlaArg: 2.97 ± 0.072
4.235AlaSer: 4.235 ± 0.081
3.939AlaThr: 3.939 ± 0.083
5.412AlaVal: 5.412 ± 0.097
0.606AlaTrp: 0.606 ± 0.034
2.289AlaTyr: 2.289 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.476CysAla: 0.476 ± 0.03
0.068CysCys: 0.068 ± 0.011
0.353CysAsp: 0.353 ± 0.025
0.271CysGlu: 0.271 ± 0.019
0.275CysPhe: 0.275 ± 0.023
0.556CysGly: 0.556 ± 0.036
0.187CysHis: 0.187 ± 0.016
0.296CysIle: 0.296 ± 0.022
0.27CysLys: 0.27 ± 0.022
0.637CysLeu: 0.637 ± 0.033
0.124CysMet: 0.124 ± 0.013
0.192CysAsn: 0.192 ± 0.017
0.278CysPro: 0.278 ± 0.026
0.278CysGln: 0.278 ± 0.024
0.247CysArg: 0.247 ± 0.02
0.403CysSer: 0.403 ± 0.025
0.273CysThr: 0.273 ± 0.018
0.33CysVal: 0.33 ± 0.028
0.06CysTrp: 0.06 ± 0.01
0.229CysTyr: 0.229 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.573AspAla: 3.573 ± 0.074
0.301AspCys: 0.301 ± 0.022
3.419AspAsp: 3.419 ± 0.091
4.131AspGlu: 4.131 ± 0.094
2.986AspPhe: 2.986 ± 0.067
3.508AspGly: 3.508 ± 0.085
1.369AspHis: 1.369 ± 0.047
3.895AspIle: 3.895 ± 0.088
3.98AspLys: 3.98 ± 0.084
6.015AspLeu: 6.015 ± 0.11
1.328AspMet: 1.328 ± 0.045
2.625AspAsn: 2.625 ± 0.075
2.721AspPro: 2.721 ± 0.07
3.215AspGln: 3.215 ± 0.079
2.284AspArg: 2.284 ± 0.07
3.368AspSer: 3.368 ± 0.079
2.676AspThr: 2.676 ± 0.072
3.963AspVal: 3.963 ± 0.089
0.598AspTrp: 0.598 ± 0.032
2.398AspTyr: 2.398 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
3.913GluAla: 3.913 ± 0.087
0.27GluCys: 0.27 ± 0.023
3.133GluAsp: 3.133 ± 0.076
3.934GluGlu: 3.934 ± 0.091
2.369GluPhe: 2.369 ± 0.064
2.794GluGly: 2.794 ± 0.068
1.261GluHis: 1.261 ± 0.047
4.548GluIle: 4.548 ± 0.106
5.573GluLys: 5.573 ± 0.104
6.293GluLeu: 6.293 ± 0.117
2.053GluMet: 2.053 ± 0.058
3.362GluAsn: 3.362 ± 0.07
1.832GluPro: 1.832 ± 0.059
3.393GluGln: 3.393 ± 0.092
2.648GluArg: 2.648 ± 0.076
2.882GluSer: 2.882 ± 0.069
3.227GluThr: 3.227 ± 0.072
3.931GluVal: 3.931 ± 0.088
0.558GluTrp: 0.558 ± 0.028
1.987GluTyr: 1.987 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.409PheAla: 3.409 ± 0.075
0.369PheCys: 0.369 ± 0.029
2.822PheAsp: 2.822 ± 0.071
2.466PheGlu: 2.466 ± 0.064
2.133PhePhe: 2.133 ± 0.062
3.15PheGly: 3.15 ± 0.078
0.784PheHis: 0.784 ± 0.037
2.977PheIle: 2.977 ± 0.076
2.809PheLys: 2.809 ± 0.072
4.079PheLeu: 4.079 ± 0.087
1.164PheMet: 1.164 ± 0.045
2.134PheAsn: 2.134 ± 0.06
1.526PhePro: 1.526 ± 0.05
1.45PheGln: 1.45 ± 0.05
1.3PheArg: 1.3 ± 0.046
3.376PheSer: 3.376 ± 0.073
2.378PheThr: 2.378 ± 0.063
3.149PheVal: 3.149 ± 0.076
0.507PheTrp: 0.507 ± 0.03
1.661PheTyr: 1.661 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.729GlyAla: 4.729 ± 0.104
0.411GlyCys: 0.411 ± 0.026
3.29GlyAsp: 3.29 ± 0.074
3.555GlyGlu: 3.555 ± 0.081
3.081GlyPhe: 3.081 ± 0.066
4.451GlyGly: 4.451 ± 0.115
1.567GlyHis: 1.567 ± 0.052
5.199GlyIle: 5.199 ± 0.094
4.935GlyLys: 4.935 ± 0.098
6.441GlyLeu: 6.441 ± 0.113
1.832GlyMet: 1.832 ± 0.062
2.804GlyAsn: 2.804 ± 0.078
1.613GlyPro: 1.613 ± 0.052
3.333GlyGln: 3.333 ± 0.076
2.653GlyArg: 2.653 ± 0.077
4.207GlySer: 4.207 ± 0.081
3.996GlyThr: 3.996 ± 0.085
4.787GlyVal: 4.787 ± 0.084
0.697GlyTrp: 0.697 ± 0.036
2.694GlyTyr: 2.694 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.379HisAla: 1.379 ± 0.05
0.145HisCys: 0.145 ± 0.015
1.226HisAsp: 1.226 ± 0.044
1.268HisGlu: 1.268 ± 0.043
1.047HisPhe: 1.047 ± 0.044
1.533HisGly: 1.533 ± 0.05
0.592HisHis: 0.592 ± 0.033
1.209HisIle: 1.209 ± 0.039
1.192HisLys: 1.192 ± 0.047
2.074HisLeu: 2.074 ± 0.06
0.48HisMet: 0.48 ± 0.03
0.891HisAsn: 0.891 ± 0.034
0.914HisPro: 0.914 ± 0.037
0.983HisGln: 0.983 ± 0.041
0.873HisArg: 0.873 ± 0.035
1.127HisSer: 1.127 ± 0.047
0.985HisThr: 0.985 ± 0.041
1.374HisVal: 1.374 ± 0.048
0.215HisTrp: 0.215 ± 0.019
0.914HisTyr: 0.914 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.628IleAla: 5.628 ± 0.105
0.481IleCys: 0.481 ± 0.027
4.423IleAsp: 4.423 ± 0.089
3.871IleGlu: 3.871 ± 0.084
3.108IlePhe: 3.108 ± 0.089
4.983IleGly: 4.983 ± 0.097
1.271IleHis: 1.271 ± 0.044
4.626IleIle: 4.626 ± 0.099
4.932IleLys: 4.932 ± 0.085
6.379IleLeu: 6.379 ± 0.13
1.733IleMet: 1.733 ± 0.053
3.308IleAsn: 3.308 ± 0.082
2.721IlePro: 2.721 ± 0.067
2.409IleGln: 2.409 ± 0.068
2.482IleArg: 2.482 ± 0.07
4.825IleSer: 4.825 ± 0.101
3.957IleThr: 3.957 ± 0.081
4.817IleVal: 4.817 ± 0.1
0.614IleTrp: 0.614 ± 0.029
2.258IleTyr: 2.258 ± 0.055
0.003IleXaa: 0.003 ± 0.002
Lys
4.761LysAla: 4.761 ± 0.078
0.283LysCys: 0.283 ± 0.024
4.188LysAsp: 4.188 ± 0.082
4.93LysGlu: 4.93 ± 0.109
2.451LysPhe: 2.451 ± 0.065
3.532LysGly: 3.532 ± 0.07
1.367LysHis: 1.367 ± 0.052
5.265LysIle: 5.265 ± 0.101
6.777LysLys: 6.777 ± 0.137
6.385LysLeu: 6.385 ± 0.105
2.562LysMet: 2.562 ± 0.056
4.324LysAsn: 4.324 ± 0.099
2.17LysPro: 2.17 ± 0.061
4.025LysGln: 4.025 ± 0.087
3.441LysArg: 3.441 ± 0.088
3.583LysSer: 3.583 ± 0.08
4.344LysThr: 4.344 ± 0.085
4.666LysVal: 4.666 ± 0.095
0.697LysTrp: 0.697 ± 0.036
2.941LysTyr: 2.941 ± 0.075
0.002LysXaa: 0.002 ± 0.002
Leu
8.309LeuAla: 8.309 ± 0.134
0.662LeuCys: 0.662 ± 0.033
5.55LeuAsp: 5.55 ± 0.102
5.225LeuGlu: 5.225 ± 0.105
4.136LeuPhe: 4.136 ± 0.097
6.517LeuGly: 6.517 ± 0.132
1.8LeuHis: 1.8 ± 0.053
6.39LeuIle: 6.39 ± 0.131
6.936LeuLys: 6.936 ± 0.111
9.694LeuLeu: 9.694 ± 0.17
2.299LeuMet: 2.299 ± 0.061
5.026LeuAsn: 5.026 ± 0.086
4.077LeuPro: 4.077 ± 0.081
4.23LeuGln: 4.23 ± 0.08
3.755LeuArg: 3.755 ± 0.088
6.662LeuSer: 6.662 ± 0.106
6.449LeuThr: 6.449 ± 0.098
6.821LeuVal: 6.821 ± 0.098
0.753LeuTrp: 0.753 ± 0.042
2.781LeuTyr: 2.781 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
2.016MetAla: 2.016 ± 0.057
0.117MetCys: 0.117 ± 0.012
1.484MetAsp: 1.484 ± 0.045
1.406MetGlu: 1.406 ± 0.043
1.021MetPhe: 1.021 ± 0.047
1.78MetGly: 1.78 ± 0.058
0.509MetHis: 0.509 ± 0.028
2.042MetIle: 2.042 ± 0.059
1.954MetLys: 1.954 ± 0.046
2.385MetLeu: 2.385 ± 0.061
0.717MetMet: 0.717 ± 0.029
1.44MetAsn: 1.44 ± 0.057
1.053MetPro: 1.053 ± 0.037
1.117MetGln: 1.117 ± 0.043
0.98MetArg: 0.98 ± 0.042
1.726MetSer: 1.726 ± 0.052
1.733MetThr: 1.733 ± 0.051
1.762MetVal: 1.762 ± 0.054
0.172MetTrp: 0.172 ± 0.019
0.686MetTyr: 0.686 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.175AsnAla: 3.175 ± 0.078
0.317AsnCys: 0.317 ± 0.023
3.113AsnAsp: 3.113 ± 0.078
3.287AsnGlu: 3.287 ± 0.078
2.359AsnPhe: 2.359 ± 0.062
3.729AsnGly: 3.729 ± 0.093
0.925AsnHis: 0.925 ± 0.037
3.269AsnIle: 3.269 ± 0.076
3.497AsnLys: 3.497 ± 0.076
4.389AsnLeu: 4.389 ± 0.091
1.227AsnMet: 1.227 ± 0.039
2.684AsnAsn: 2.684 ± 0.075
1.496AsnPro: 1.496 ± 0.052
2.055AsnGln: 2.055 ± 0.062
1.622AsnArg: 1.622 ± 0.051
3.173AsnSer: 3.173 ± 0.082
2.395AsnThr: 2.395 ± 0.063
3.372AsnVal: 3.372 ± 0.074
0.582AsnTrp: 0.582 ± 0.033
2.157AsnTyr: 2.157 ± 0.063
0.0AsnXaa: 0.0 ± 0.0
Pro
2.972ProAla: 2.972 ± 0.077
0.158ProCys: 0.158 ± 0.016
2.242ProAsp: 2.242 ± 0.062
2.848ProGlu: 2.848 ± 0.081
1.63ProPhe: 1.63 ± 0.055
2.147ProGly: 2.147 ± 0.067
0.736ProHis: 0.736 ± 0.037
2.113ProIle: 2.113 ± 0.058
2.247ProLys: 2.247 ± 0.063
3.44ProLeu: 3.44 ± 0.077
0.689ProMet: 0.689 ± 0.034
1.657ProAsn: 1.657 ± 0.053
0.626ProPro: 0.626 ± 0.033
1.821ProGln: 1.821 ± 0.054
1.14ProArg: 1.14 ± 0.043
1.949ProSer: 1.949 ± 0.054
2.003ProThr: 2.003 ± 0.055
2.689ProVal: 2.689 ± 0.073
0.351ProTrp: 0.351 ± 0.027
1.313ProTyr: 1.313 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
3.871GlnAla: 3.871 ± 0.089
0.15GlnCys: 0.15 ± 0.018
2.386GlnAsp: 2.386 ± 0.069
3.243GlnGlu: 3.243 ± 0.083
1.565GlnPhe: 1.565 ± 0.052
2.489GlnGly: 2.489 ± 0.061
0.951GlnHis: 0.951 ± 0.04
3.532GlnIle: 3.532 ± 0.079
4.1GlnLys: 4.1 ± 0.092
4.703GlnLeu: 4.703 ± 0.091
1.325GlnMet: 1.325 ± 0.043
2.487GlnAsn: 2.487 ± 0.073
1.565GlnPro: 1.565 ± 0.056
3.017GlnGln: 3.017 ± 0.106
2.104GlnArg: 2.104 ± 0.06
2.33GlnSer: 2.33 ± 0.067
2.84GlnThr: 2.84 ± 0.072
3.349GlnVal: 3.349 ± 0.076
0.377GlnTrp: 0.377 ± 0.025
1.444GlnTyr: 1.444 ± 0.055
0.002GlnXaa: 0.002 ± 0.002
Arg
2.63ArgAla: 2.63 ± 0.069
0.203ArgCys: 0.203 ± 0.019
2.095ArgAsp: 2.095 ± 0.062
2.29ArgGlu: 2.29 ± 0.063
1.856ArgPhe: 1.856 ± 0.058
2.338ArgGly: 2.338 ± 0.066
0.87ArgHis: 0.87 ± 0.041
2.647ArgIle: 2.647 ± 0.068
2.985ArgLys: 2.985 ± 0.08
3.958ArgLeu: 3.958 ± 0.089
1.073ArgMet: 1.073 ± 0.04
1.908ArgAsn: 1.908 ± 0.057
1.409ArgPro: 1.409 ± 0.046
2.144ArgGln: 2.144 ± 0.063
1.85ArgArg: 1.85 ± 0.061
2.344ArgSer: 2.344 ± 0.056
2.009ArgThr: 2.009 ± 0.05
2.651ArgVal: 2.651 ± 0.065
0.33ArgTrp: 0.33 ± 0.025
1.565ArgTyr: 1.565 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
4.609SerAla: 4.609 ± 0.08
0.294SerCys: 0.294 ± 0.022
3.623SerAsp: 3.623 ± 0.086
3.65SerGlu: 3.65 ± 0.082
3.079SerPhe: 3.079 ± 0.077
4.696SerGly: 4.696 ± 0.103
1.248SerHis: 1.248 ± 0.047
3.807SerIle: 3.807 ± 0.081
4.16SerLys: 4.16 ± 0.084
6.35SerLeu: 6.35 ± 0.119
1.52SerMet: 1.52 ± 0.053
2.78SerAsn: 2.78 ± 0.074
1.923SerPro: 1.923 ± 0.058
2.918SerGln: 2.918 ± 0.085
2.396SerArg: 2.396 ± 0.07
4.269SerSer: 4.269 ± 0.109
3.258SerThr: 3.258 ± 0.077
4.223SerVal: 4.223 ± 0.088
0.719SerTrp: 0.719 ± 0.039
2.157SerTyr: 2.157 ± 0.062
0.002SerXaa: 0.002 ± 0.001
Thr
4.675ThrAla: 4.675 ± 0.106
0.293ThrCys: 0.293 ± 0.027
3.42ThrAsp: 3.42 ± 0.071
3.103ThrGlu: 3.103 ± 0.08
2.326ThrPhe: 2.326 ± 0.063
4.394ThrGly: 4.394 ± 0.081
1.068ThrHis: 1.068 ± 0.046
4.069ThrIle: 4.069 ± 0.094
3.812ThrLys: 3.812 ± 0.076
5.472ThrLeu: 5.472 ± 0.089
1.323ThrMet: 1.323 ± 0.039
2.593ThrAsn: 2.593 ± 0.07
2.424ThrPro: 2.424 ± 0.065
2.227ThrGln: 2.227 ± 0.067
1.926ThrArg: 1.926 ± 0.051
3.474ThrSer: 3.474 ± 0.082
3.342ThrThr: 3.342 ± 0.081
4.248ThrVal: 4.248 ± 0.097
0.501ThrTrp: 0.501 ± 0.032
1.824ThrTyr: 1.824 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
5.451ValAla: 5.451 ± 0.105
0.475ValCys: 0.475 ± 0.029
4.196ValAsp: 4.196 ± 0.086
3.771ValGlu: 3.771 ± 0.084
2.887ValPhe: 2.887 ± 0.077
4.787ValGly: 4.787 ± 0.087
1.287ValHis: 1.287 ± 0.045
5.296ValIle: 5.296 ± 0.107
4.862ValLys: 4.862 ± 0.091
6.751ValLeu: 6.751 ± 0.121
1.691ValMet: 1.691 ± 0.049
3.254ValAsn: 3.254 ± 0.082
2.594ValPro: 2.594 ± 0.065
2.59ValGln: 2.59 ± 0.065
2.375ValArg: 2.375 ± 0.06
4.825ValSer: 4.825 ± 0.098
4.42ValThr: 4.42 ± 0.096
5.321ValVal: 5.321 ± 0.122
0.608ValTrp: 0.608 ± 0.031
2.219ValTyr: 2.219 ± 0.062
0.005ValXaa: 0.005 ± 0.003
Trp
0.538TrpAla: 0.538 ± 0.036
0.072TrpCys: 0.072 ± 0.012
0.501TrpAsp: 0.501 ± 0.031
0.406TrpGlu: 0.406 ± 0.021
0.489TrpPhe: 0.489 ± 0.031
0.61TrpGly: 0.61 ± 0.036
0.221TrpHis: 0.221 ± 0.02
0.601TrpIle: 0.601 ± 0.037
0.543TrpLys: 0.543 ± 0.028
1.232TrpLeu: 1.232 ± 0.049
0.289TrpMet: 0.289 ± 0.021
0.458TrpAsn: 0.458 ± 0.03
0.27TrpPro: 0.27 ± 0.023
0.621TrpGln: 0.621 ± 0.034
0.382TrpArg: 0.382 ± 0.028
0.595TrpSer: 0.595 ± 0.037
0.494TrpThr: 0.494 ± 0.029
0.618TrpVal: 0.618 ± 0.033
0.114TrpTrp: 0.114 ± 0.015
0.397TrpTyr: 0.397 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.294TyrAla: 2.294 ± 0.069
0.294TyrCys: 0.294 ± 0.024
2.123TyrAsp: 2.123 ± 0.066
1.928TyrGlu: 1.928 ± 0.052
1.809TyrPhe: 1.809 ± 0.062
2.539TyrGly: 2.539 ± 0.07
0.969TyrHis: 0.969 ± 0.04
2.092TyrIle: 2.092 ± 0.054
1.629TyrLys: 1.629 ± 0.054
3.991TyrLeu: 3.991 ± 0.095
0.792TyrMet: 0.792 ± 0.036
1.525TyrAsn: 1.525 ± 0.054
1.34TyrPro: 1.34 ± 0.042
2.162TyrGln: 2.162 ± 0.07
1.728TyrArg: 1.728 ± 0.046
2.326TyrSer: 2.326 ± 0.059
1.798TyrThr: 1.798 ± 0.055
2.185TyrVal: 2.185 ± 0.061
0.39TyrTrp: 0.39 ± 0.026
1.442TyrTyr: 1.442 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.002XaaAsp: 0.002 ± 0.002
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.002XaaIle: 0.002 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.003XaaLeu: 0.003 ± 0.002
0.002XaaMet: 0.002 ± 0.002
0.002XaaAsn: 0.002 ± 0.002
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.002XaaTrp: 0.002 ± 0.001
0.0XaaTyr: 0.0 ± 0.0
1.325XaaXaa: 1.325 ± 0.614
Statistics based on 2127 proteins (615153 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski