candidate division MSBL1 archaeon SCGC-AAA382F02

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VIZ1|A0A133VIZ1_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382F02 OX=1698282 GN=AKJ53_00445 PE=4 SV=1
MM1 pKa = 7.23ATDD4 pKa = 4.34PVCGMEE10 pKa = 4.25VDD12 pKa = 4.09PEE14 pKa = 3.96EE15 pKa = 5.18APAEE19 pKa = 4.27TKK21 pKa = 10.7HH22 pKa = 5.99EE23 pKa = 4.33GEE25 pKa = 4.62TIYY28 pKa = 10.58FCCSVCKK35 pKa = 10.36EE36 pKa = 4.03KK37 pKa = 11.14FEE39 pKa = 4.62EE40 pKa = 4.27NPEE43 pKa = 3.93KK44 pKa = 10.91FMM46 pKa = 6.46

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VIB5|A0A133VIB5_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382F02 OX=1698282 GN=AKJ53_01115 PE=4 SV=1
MM1 pKa = 7.63PCRR4 pKa = 11.84VGITTRR10 pKa = 11.84PEE12 pKa = 3.19KK13 pKa = 10.28RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.11WEE19 pKa = 3.92NKK21 pKa = 9.92VIGLKK26 pKa = 8.8NWRR29 pKa = 11.84IIGKK33 pKa = 8.31HH34 pKa = 4.41QSRR37 pKa = 11.84KK38 pKa = 9.29KK39 pKa = 9.84AQEE42 pKa = 4.24HH43 pKa = 5.91EE44 pKa = 4.03DD45 pKa = 3.51SYY47 pKa = 11.93ARR49 pKa = 11.84QHH51 pKa = 6.24GCGAMHH57 pKa = 7.46GGRR60 pKa = 11.84WGSGMYY66 pKa = 9.49YY67 pKa = 10.36VYY69 pKa = 10.67RR70 pKa = 11.84FDD72 pKa = 3.63YY73 pKa = 10.72TRR75 pKa = 11.84TKK77 pKa = 10.98

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

344

0

344

90793

43

1258

263.9

29.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.495 ± 0.15

0.897 ± 0.052

5.586 ± 0.104

10.371 ± 0.174

3.598 ± 0.101

7.454 ± 0.133

1.772 ± 0.05

7.302 ± 0.114

7.83 ± 0.135

9.236 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.052

4.021 ± 0.111

4.081 ± 0.082

2.459 ± 0.076

4.747 ± 0.114

6.546 ± 0.111

5.035 ± 0.125

6.765 ± 0.105

0.969 ± 0.048

2.769 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski