Corynebacterium phocae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7D2N2|A0A1L7D2N2_9CORY RNA polymerase sigma factor SigA OS=Corynebacterium phocae OX=161895 GN=sigA PE=3 SV=1
MM1 pKa = 7.53SNLPANYY8 pKa = 9.6SYY10 pKa = 11.52SEE12 pKa = 3.85DD13 pKa = 4.36HH14 pKa = 6.41EE15 pKa = 5.04WIDD18 pKa = 3.81APAAEE23 pKa = 4.58VAGKK27 pKa = 6.59TLKK30 pKa = 10.97VGITSVAADD39 pKa = 3.73RR40 pKa = 11.84LGEE43 pKa = 4.25VVFAEE48 pKa = 4.31LPEE51 pKa = 4.52VGDD54 pKa = 3.7AVTAGEE60 pKa = 4.3TCGEE64 pKa = 4.21VEE66 pKa = 4.36STKK69 pKa = 10.7SVSDD73 pKa = 4.05LYY75 pKa = 11.59SPVTGTVTAVNDD87 pKa = 4.18AVHH90 pKa = 7.45DD91 pKa = 4.68DD92 pKa = 3.72YY93 pKa = 12.18SLINSDD99 pKa = 3.54PFEE102 pKa = 4.89AGWLFEE108 pKa = 4.43VEE110 pKa = 4.14VSEE113 pKa = 6.02AGDD116 pKa = 3.5LMTAEE121 pKa = 5.39EE122 pKa = 4.54YY123 pKa = 10.92ASQNGVV129 pKa = 2.85

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7D282|A0A1L7D282_9CORY Histidinol dehydrogenase OS=Corynebacterium phocae OX=161895 GN=hisD PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.38KK3 pKa = 10.05FRR5 pKa = 11.84EE6 pKa = 4.12WPGNFKK12 pKa = 10.6FAVVCGAAAVLAGVFLLCIGQSGMDD37 pKa = 3.43YY38 pKa = 11.6AMAGVAIAGGLVVVLGAPAWGLNDD62 pKa = 3.53HH63 pKa = 6.94EE64 pKa = 4.35EE65 pKa = 3.82TARR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.55RR71 pKa = 11.84ARR73 pKa = 11.84QARR76 pKa = 11.84AEE78 pKa = 3.94LRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 3.56

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2229

0

2229

732272

40

3266

328.5

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.715 ± 0.085

0.69 ± 0.013

5.915 ± 0.047

6.352 ± 0.052

3.457 ± 0.033

8.4 ± 0.044

2.106 ± 0.023

4.878 ± 0.036

3.774 ± 0.045

9.463 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.023

3.028 ± 0.027

5.226 ± 0.044

3.47 ± 0.032

5.903 ± 0.051

5.747 ± 0.041

5.931 ± 0.042

8.153 ± 0.05

1.356 ± 0.019

2.296 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski