Pleomorphomonas diazotrophica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Pleomorphomonadaceae; Pleomorphomonas

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4TQB3|A0A1I4TQB3_9RHIZ Oligopeptide ABC transporter ATP-binding protein OppF OS=Pleomorphomonas diazotrophica OX=1166257 GN=CXZ10_17875 PE=3 SV=1
MM1 pKa = 7.37NFQMQASALAAAIFVMCVADD21 pKa = 4.23ASAYY25 pKa = 9.32DD26 pKa = 3.57RR27 pKa = 11.84RR28 pKa = 11.84VVVVNNTSTTIQEE41 pKa = 4.8FYY43 pKa = 11.32ASNTGQNDD51 pKa = 3.12WQEE54 pKa = 4.67DD55 pKa = 3.8IFGEE59 pKa = 4.69GALPPGGEE67 pKa = 4.0VTINIDD73 pKa = 4.88DD74 pKa = 4.05GSGYY78 pKa = 10.45CKK80 pKa = 10.4YY81 pKa = 10.79DD82 pKa = 3.6FRR84 pKa = 11.84AVFEE88 pKa = 4.95DD89 pKa = 3.39ATEE92 pKa = 4.14TTKK95 pKa = 11.12AGVNVCEE102 pKa = 4.15VGRR105 pKa = 11.84FSFEE109 pKa = 3.75EE110 pKa = 4.04

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4TCE9|A0A1I4TCE9_9RHIZ Acetylglutamate kinase OS=Pleomorphomonas diazotrophica OX=1166257 GN=argB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 10.24LIRR12 pKa = 11.84KK13 pKa = 8.58RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.18GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.16RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4175

0

4175

1319347

29

2007

316.0

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.04 ± 0.053

0.735 ± 0.011

5.871 ± 0.031

5.679 ± 0.037

3.715 ± 0.026

8.666 ± 0.034

1.847 ± 0.019

5.183 ± 0.028

3.184 ± 0.033

10.365 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.018

2.362 ± 0.019

5.109 ± 0.032

2.597 ± 0.021

7.107 ± 0.04

5.455 ± 0.03

5.376 ± 0.026

7.95 ± 0.033

1.238 ± 0.015

2.138 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski