Desmodium mottle virus
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6GNC2|A0A1S6GNC2_9GEMI Transcriptional activator protein OS=Desmodium mottle virus OX=1960710 GN=AC2 PE=3 SV=1
MM1 pKa = 8.01 WDD3 pKa = 3.63 PLEE6 pKa = 4.87 HH7 pKa = 7.03 PFPHH11 pKa = 5.63 TVYY14 pKa = 10.66 GVRR17 pKa = 11.84 CMLAVKK23 pKa = 9.85 YY24 pKa = 9.32 VQLVIATYY32 pKa = 9.27 PVDD35 pKa = 4.96 SIGEE39 pKa = 4.0 DD40 pKa = 3.28 LLRR43 pKa = 11.84 RR44 pKa = 11.84 LIQILRR50 pKa = 11.84 CRR52 pKa = 11.84 NHH54 pKa = 7.13 DD55 pKa = 3.79 EE56 pKa = 4.26 AEE58 pKa = 3.95 LRR60 pKa = 11.84 YY61 pKa = 9.87 SLLYY65 pKa = 10.72 ADD67 pKa = 4.56 VEE69 pKa = 4.31 RR70 pKa = 11.84 TEE72 pKa = 5.36 ASDD75 pKa = 3.68 LRR77 pKa = 11.84 NPSGAPCTCRR87 pKa = 11.84 SCPKK91 pKa = 10.08 HH92 pKa = 5.81 VQTKK96 pKa = 9.74 GLEE99 pKa = 4.29 EE100 pKa = 4.12 PAHH103 pKa = 4.94 VQEE106 pKa = 4.73 AQDD109 pKa = 3.57 IQGLPFKK116 pKa = 10.88
Molecular weight: 13.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.581
IPC2_protein 5.652
IPC_protein 5.652
Toseland 6.008
ProMoST 6.008
Dawson 5.919
Bjellqvist 5.919
Wikipedia 5.919
Rodwell 5.906
Grimsley 6.122
Solomon 5.919
Lehninger 5.906
Nozaki 6.173
DTASelect 6.364
Thurlkill 6.376
EMBOSS 6.338
Sillero 6.287
Patrickios 3.694
IPC_peptide 5.931
IPC2_peptide 6.3
IPC2.peptide.svr19 6.229
Protein with the highest isoelectric point:
>tr|A0A1S6GNC0|A0A1S6GNC0_9GEMI Replication enhancer OS=Desmodium mottle virus OX=1960710 GN=AC3 PE=3 SV=1
MM1 pKa = 7.62 TKK3 pKa = 10.23 RR4 pKa = 11.84 SYY6 pKa = 10.09 DD7 pKa = 3.64 TAFSTPMSSARR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LTFATPLALPAPAGAAPNTYY40 pKa = 9.73 KK41 pKa = 10.45 RR42 pKa = 11.84 RR43 pKa = 11.84 AWRR46 pKa = 11.84 NRR48 pKa = 11.84 PMYY51 pKa = 9.61 RR52 pKa = 11.84 KK53 pKa = 8.33 PKK55 pKa = 8.79 IYY57 pKa = 9.82 RR58 pKa = 11.84 VYY60 pKa = 10.48 RR61 pKa = 11.84 SSDD64 pKa = 3.43 VPKK67 pKa = 10.71 GCEE70 pKa = 4.37 GPCKK74 pKa = 9.67 VQSYY78 pKa = 8.25 DD79 pKa = 3.29 QRR81 pKa = 11.84 FDD83 pKa = 3.74 CKK85 pKa = 9.81 HH86 pKa = 5.9 TGSVLCVSDD95 pKa = 3.2 ITRR98 pKa = 11.84 GSGLTHH104 pKa = 6.46 RR105 pKa = 11.84 VGKK108 pKa = 9.73 RR109 pKa = 11.84 FCVKK113 pKa = 10.47 SIMFRR118 pKa = 11.84 GKK120 pKa = 9.32 VWMDD124 pKa = 3.72 DD125 pKa = 3.73 NIKK128 pKa = 10.67 SKK130 pKa = 10.64 SHH132 pKa = 6.03 TNHH135 pKa = 4.9 VMFFLVRR142 pKa = 11.84 DD143 pKa = 3.73 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 8.72 GTPPDD152 pKa = 4.16 FGQVFNMFDD161 pKa = 3.83 NEE163 pKa = 4.14 PTTATVKK170 pKa = 10.46 QDD172 pKa = 2.95 FRR174 pKa = 11.84 DD175 pKa = 3.43 RR176 pKa = 11.84 FQVKK180 pKa = 8.84 RR181 pKa = 11.84 RR182 pKa = 11.84 WWVGVTGGQYY192 pKa = 10.8 ASKK195 pKa = 10.16 EE196 pKa = 3.73 QAIVNKK202 pKa = 9.67 FVYY205 pKa = 10.3 LNNYY209 pKa = 7.71 VVYY212 pKa = 10.25 NHH214 pKa = 6.0 QEE216 pKa = 3.43 AGKK219 pKa = 10.41 YY220 pKa = 8.39 EE221 pKa = 4.09 NHH223 pKa = 6.65 TEE225 pKa = 3.89 NAMLLYY231 pKa = 8.4 MACTHH236 pKa = 7.07 ASNPVYY242 pKa = 9.77 ATLKK246 pKa = 9.27 IRR248 pKa = 11.84 VYY250 pKa = 10.62 FYY252 pKa = 11.36 DD253 pKa = 4.21 SIGNN257 pKa = 3.69
Molecular weight: 29.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.28
IPC2_protein 9.531
IPC_protein 9.736
Toseland 9.984
ProMoST 9.78
Dawson 10.233
Bjellqvist 9.97
Wikipedia 10.438
Rodwell 10.54
Grimsley 10.335
Solomon 10.262
Lehninger 10.218
Nozaki 10.028
DTASelect 9.955
Thurlkill 10.072
EMBOSS 10.409
Sillero 10.16
Patrickios 9.575
IPC_peptide 10.262
IPC2_peptide 8.887
IPC2.peptide.svr19 8.463
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1619
58
358
202.4
23.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.621 ± 0.694
2.347 ± 0.27
4.756 ± 0.222
5.065 ± 0.614
4.262 ± 0.445
5.435 ± 0.516
3.335 ± 0.423
5.312 ± 0.623
6.115 ± 0.745
7.474 ± 0.858
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.325
4.571 ± 0.582
5.374 ± 0.579
4.324 ± 0.44
7.041 ± 1.014
8.833 ± 1.184
6.177 ± 0.535
6.115 ± 0.668
1.297 ± 0.17
4.2 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here