Desmodium mottle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S6GNC2|A0A1S6GNC2_9GEMI Transcriptional activator protein OS=Desmodium mottle virus OX=1960710 GN=AC2 PE=3 SV=1
MM1 pKa = 8.01WDD3 pKa = 3.63PLEE6 pKa = 4.87HH7 pKa = 7.03PFPHH11 pKa = 5.63TVYY14 pKa = 10.66GVRR17 pKa = 11.84CMLAVKK23 pKa = 9.85YY24 pKa = 9.32VQLVIATYY32 pKa = 9.27PVDD35 pKa = 4.96SIGEE39 pKa = 4.0DD40 pKa = 3.28LLRR43 pKa = 11.84RR44 pKa = 11.84LIQILRR50 pKa = 11.84CRR52 pKa = 11.84NHH54 pKa = 7.13DD55 pKa = 3.79EE56 pKa = 4.26AEE58 pKa = 3.95LRR60 pKa = 11.84YY61 pKa = 9.87SLLYY65 pKa = 10.72ADD67 pKa = 4.56VEE69 pKa = 4.31RR70 pKa = 11.84TEE72 pKa = 5.36ASDD75 pKa = 3.68LRR77 pKa = 11.84NPSGAPCTCRR87 pKa = 11.84SCPKK91 pKa = 10.08HH92 pKa = 5.81VQTKK96 pKa = 9.74GLEE99 pKa = 4.29EE100 pKa = 4.12PAHH103 pKa = 4.94VQEE106 pKa = 4.73AQDD109 pKa = 3.57IQGLPFKK116 pKa = 10.88

Molecular weight:
13.26 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S6GNC0|A0A1S6GNC0_9GEMI Replication enhancer OS=Desmodium mottle virus OX=1960710 GN=AC3 PE=3 SV=1
MM1 pKa = 7.62TKK3 pKa = 10.23RR4 pKa = 11.84SYY6 pKa = 10.09DD7 pKa = 3.64TAFSTPMSSARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LTFATPLALPAPAGAAPNTYY40 pKa = 9.73KK41 pKa = 10.45RR42 pKa = 11.84RR43 pKa = 11.84AWRR46 pKa = 11.84NRR48 pKa = 11.84PMYY51 pKa = 9.61RR52 pKa = 11.84KK53 pKa = 8.33PKK55 pKa = 8.79IYY57 pKa = 9.82RR58 pKa = 11.84VYY60 pKa = 10.48RR61 pKa = 11.84SSDD64 pKa = 3.43VPKK67 pKa = 10.71GCEE70 pKa = 4.37GPCKK74 pKa = 9.67VQSYY78 pKa = 8.25DD79 pKa = 3.29QRR81 pKa = 11.84FDD83 pKa = 3.74CKK85 pKa = 9.81HH86 pKa = 5.9TGSVLCVSDD95 pKa = 3.2ITRR98 pKa = 11.84GSGLTHH104 pKa = 6.46RR105 pKa = 11.84VGKK108 pKa = 9.73RR109 pKa = 11.84FCVKK113 pKa = 10.47SIMFRR118 pKa = 11.84GKK120 pKa = 9.32VWMDD124 pKa = 3.72DD125 pKa = 3.73NIKK128 pKa = 10.67SKK130 pKa = 10.64SHH132 pKa = 6.03TNHH135 pKa = 4.9VMFFLVRR142 pKa = 11.84DD143 pKa = 3.73RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 8.72GTPPDD152 pKa = 4.16FGQVFNMFDD161 pKa = 3.83NEE163 pKa = 4.14PTTATVKK170 pKa = 10.46QDD172 pKa = 2.95FRR174 pKa = 11.84DD175 pKa = 3.43RR176 pKa = 11.84FQVKK180 pKa = 8.84RR181 pKa = 11.84RR182 pKa = 11.84WWVGVTGGQYY192 pKa = 10.8ASKK195 pKa = 10.16EE196 pKa = 3.73QAIVNKK202 pKa = 9.67FVYY205 pKa = 10.3LNNYY209 pKa = 7.71VVYY212 pKa = 10.25NHH214 pKa = 6.0QEE216 pKa = 3.43AGKK219 pKa = 10.41YY220 pKa = 8.39EE221 pKa = 4.09NHH223 pKa = 6.65TEE225 pKa = 3.89NAMLLYY231 pKa = 8.4MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.77ATLKK246 pKa = 9.27IRR248 pKa = 11.84VYY250 pKa = 10.62FYY252 pKa = 11.36DD253 pKa = 4.21SIGNN257 pKa = 3.69

Molecular weight:
29.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1619

58

358

202.4

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 0.694

2.347 ± 0.27

4.756 ± 0.222

5.065 ± 0.614

4.262 ± 0.445

5.435 ± 0.516

3.335 ± 0.423

5.312 ± 0.623

6.115 ± 0.745

7.474 ± 0.858

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.325

4.571 ± 0.582

5.374 ± 0.579

4.324 ± 0.44

7.041 ± 1.014

8.833 ± 1.184

6.177 ± 0.535

6.115 ± 0.668

1.297 ± 0.17

4.2 ± 0.576

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski