Cronobacter phage PhiCS01 
Average proteome isoelectric point is 6.43 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A3B8E0M1|A0A3B8E0M1_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_061 PE=4 SV=1MM1 pKa = 7.59  CEE3 pKa = 3.65  KK4 pKa = 10.49  HH5 pKa = 6.9  DD6 pKa = 4.45  DD7 pKa = 3.61  EE8 pKa = 6.04  FFFRR12 pKa = 11.84  LHH14 pKa = 5.15  QAEE17 pKa = 4.24  EE18 pKa = 4.04  AGLNQEE24 pKa = 4.02  WALKK28 pKa = 9.35  VAYY31 pKa = 10.6  LEE33 pKa = 4.32  VTLDD37 pKa = 3.61  EE38 pKa = 5.09  ALGAMDD44 pKa = 5.01  MEE46 pKa = 5.56  AEE48 pKa = 4.55  SEE50 pKa = 3.98  FDD52 pKa = 4.06  PNFVMCDD59 pKa = 3.48  GVIDD63 pKa = 4.71  ADD65 pKa = 4.04  DD66 pKa = 5.87  GYY68 pKa = 11.53  DD69 pKa = 3.47  CGCRR73 pKa = 11.84  GGDD76 pKa = 3.55  ACCEE80 pKa = 4.02  CMPFF84 pKa = 4.21  
 9.42 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.829 
IPC2_protein 3.732 
IPC_protein 3.694 
Toseland    3.49 
ProMoST     3.859 
Dawson      3.681 
Bjellqvist  3.834 
Wikipedia   3.605 
Rodwell     3.528 
Grimsley    3.401 
Solomon     3.656 
Lehninger   3.617 
Nozaki      3.808 
DTASelect   3.986 
Thurlkill   3.554 
EMBOSS      3.617 
Sillero     3.808 
Patrickios  0.248 
IPC_peptide 3.656 
IPC2_peptide  3.783 
IPC2.peptide.svr19  3.734 
 Protein with the highest isoelectric point: 
>tr|A0A3B8DJJ9|A0A3B8DJJ9_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_062 PE=4 SV=1MM1 pKa = 8.54  VMIAVVVAGMRR12 pKa = 11.84  AANVCRR18 pKa = 11.84  FEE20 pKa = 5.24  VIMSKK25 pKa = 10.63  PKK27 pKa = 10.3  VKK29 pKa = 10.25  PIRR32 pKa = 11.84  ADD34 pKa = 3.35  QIKK37 pKa = 9.96  NGDD40 pKa = 3.99  WIFVPVRR47 pKa = 11.84  GGGLSKK53 pKa = 10.83  KK54 pKa = 8.85  PWYY57 pKa = 9.46  VAAARR62 pKa = 11.84  RR63 pKa = 11.84  QRR65 pKa = 11.84  ILGHH69 pKa = 6.47  IEE71 pKa = 3.47  IFVLHH76 pKa = 6.23  GEE78 pKa = 4.54  RR79 pKa = 11.84  YY80 pKa = 9.29  DD81 pKa = 3.91  KK82 pKa = 10.73  PSQRR86 pKa = 11.84  LPVYY90 pKa = 9.91  EE91 pKa = 4.01  SEE93 pKa = 5.32  KK94 pKa = 9.89  IFKK97 pKa = 10.47  VII99 pKa = 4.2  
 11.31 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.252 
IPC2_protein 9.604 
IPC_protein 9.853 
Toseland    10.555 
ProMoST     10.101 
Dawson      10.657 
Bjellqvist  10.277 
Wikipedia   10.789 
Rodwell     11.111 
Grimsley    10.687 
Solomon     10.701 
Lehninger   10.687 
Nozaki      10.526 
DTASelect   10.277 
Thurlkill   10.54 
EMBOSS      10.935 
Sillero     10.57 
Patrickios  10.862 
IPC_peptide 10.716 
IPC2_peptide  8.946 
IPC2.peptide.svr19  8.661 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        75 
0
75 
14501
54
1141
193.3
21.56
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.31 ± 0.617
1.255 ± 0.168
6.213 ± 0.266
6.675 ± 0.284
3.689 ± 0.18
6.986 ± 0.284
1.862 ± 0.235
6.11 ± 0.201
6.579 ± 0.257
7.103 ± 0.237
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        3.048 ± 0.201
4.476 ± 0.29
3.448 ± 0.211
4.02 ± 0.284
5.517 ± 0.289
6.848 ± 0.317
5.227 ± 0.345
6.889 ± 0.268
1.386 ± 0.129
3.358 ± 0.179
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here