Cronobacter phage PhiCS01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Gyeonggidovirus; Cronobacter virus PhiCS01

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3B8E0M1|A0A3B8E0M1_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_061 PE=4 SV=1
MM1 pKa = 7.59CEE3 pKa = 3.65KK4 pKa = 10.49HH5 pKa = 6.9DD6 pKa = 4.45DD7 pKa = 3.61EE8 pKa = 6.04FFFRR12 pKa = 11.84LHH14 pKa = 5.15QAEE17 pKa = 4.24EE18 pKa = 4.04AGLNQEE24 pKa = 4.02WALKK28 pKa = 9.35VAYY31 pKa = 10.6LEE33 pKa = 4.32VTLDD37 pKa = 3.61EE38 pKa = 5.09ALGAMDD44 pKa = 5.01MEE46 pKa = 5.56AEE48 pKa = 4.55SEE50 pKa = 3.98FDD52 pKa = 4.06PNFVMCDD59 pKa = 3.48GVIDD63 pKa = 4.71ADD65 pKa = 4.04DD66 pKa = 5.87GYY68 pKa = 11.53DD69 pKa = 3.47CGCRR73 pKa = 11.84GGDD76 pKa = 3.55ACCEE80 pKa = 4.02CMPFF84 pKa = 4.21

Molecular weight:
9.42 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3B8DJJ9|A0A3B8DJJ9_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_062 PE=4 SV=1
MM1 pKa = 8.54VMIAVVVAGMRR12 pKa = 11.84AANVCRR18 pKa = 11.84FEE20 pKa = 5.24VIMSKK25 pKa = 10.63PKK27 pKa = 10.3VKK29 pKa = 10.25PIRR32 pKa = 11.84ADD34 pKa = 3.35QIKK37 pKa = 9.96NGDD40 pKa = 3.99WIFVPVRR47 pKa = 11.84GGGLSKK53 pKa = 10.83KK54 pKa = 8.85PWYY57 pKa = 9.46VAAARR62 pKa = 11.84RR63 pKa = 11.84QRR65 pKa = 11.84ILGHH69 pKa = 6.47IEE71 pKa = 3.47IFVLHH76 pKa = 6.23GEE78 pKa = 4.54RR79 pKa = 11.84YY80 pKa = 9.29DD81 pKa = 3.91KK82 pKa = 10.73PSQRR86 pKa = 11.84LPVYY90 pKa = 9.91EE91 pKa = 4.01SEE93 pKa = 5.32KK94 pKa = 9.89IFKK97 pKa = 10.47VII99 pKa = 4.2

Molecular weight:
11.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14501

54

1141

193.3

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.31 ± 0.617

1.255 ± 0.168

6.213 ± 0.266

6.675 ± 0.284

3.689 ± 0.18

6.986 ± 0.284

1.862 ± 0.235

6.11 ± 0.201

6.579 ± 0.257

7.103 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.048 ± 0.201

4.476 ± 0.29

3.448 ± 0.211

4.02 ± 0.284

5.517 ± 0.289

6.848 ± 0.317

5.227 ± 0.345

6.889 ± 0.268

1.386 ± 0.129

3.358 ± 0.179

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski