Cronobacter phage PhiCS01
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B8E0M1|A0A3B8E0M1_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_061 PE=4 SV=1
MM1 pKa = 7.59 CEE3 pKa = 3.65 KK4 pKa = 10.49 HH5 pKa = 6.9 DD6 pKa = 4.45 DD7 pKa = 3.61 EE8 pKa = 6.04 FFFRR12 pKa = 11.84 LHH14 pKa = 5.15 QAEE17 pKa = 4.24 EE18 pKa = 4.04 AGLNQEE24 pKa = 4.02 WALKK28 pKa = 9.35 VAYY31 pKa = 10.6 LEE33 pKa = 4.32 VTLDD37 pKa = 3.61 EE38 pKa = 5.09 ALGAMDD44 pKa = 5.01 MEE46 pKa = 5.56 AEE48 pKa = 4.55 SEE50 pKa = 3.98 FDD52 pKa = 4.06 PNFVMCDD59 pKa = 3.48 GVIDD63 pKa = 4.71 ADD65 pKa = 4.04 DD66 pKa = 5.87 GYY68 pKa = 11.53 DD69 pKa = 3.47 CGCRR73 pKa = 11.84 GGDD76 pKa = 3.55 ACCEE80 pKa = 4.02 CMPFF84 pKa = 4.21
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.49
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.401
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 3.986
Thurlkill 3.554
EMBOSS 3.617
Sillero 3.808
Patrickios 0.248
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A3B8DJJ9|A0A3B8DJJ9_9CAUD Uncharacterized protein OS=Cronobacter phage PhiCS01 OX=2496544 GN=CS01_062 PE=4 SV=1
MM1 pKa = 8.54 VMIAVVVAGMRR12 pKa = 11.84 AANVCRR18 pKa = 11.84 FEE20 pKa = 5.24 VIMSKK25 pKa = 10.63 PKK27 pKa = 10.3 VKK29 pKa = 10.25 PIRR32 pKa = 11.84 ADD34 pKa = 3.35 QIKK37 pKa = 9.96 NGDD40 pKa = 3.99 WIFVPVRR47 pKa = 11.84 GGGLSKK53 pKa = 10.83 KK54 pKa = 8.85 PWYY57 pKa = 9.46 VAAARR62 pKa = 11.84 RR63 pKa = 11.84 QRR65 pKa = 11.84 ILGHH69 pKa = 6.47 IEE71 pKa = 3.47 IFVLHH76 pKa = 6.23 GEE78 pKa = 4.54 RR79 pKa = 11.84 YY80 pKa = 9.29 DD81 pKa = 3.91 KK82 pKa = 10.73 PSQRR86 pKa = 11.84 LPVYY90 pKa = 9.91 EE91 pKa = 4.01 SEE93 pKa = 5.32 KK94 pKa = 9.89 IFKK97 pKa = 10.47 VII99 pKa = 4.2
Molecular weight: 11.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.604
IPC_protein 9.853
Toseland 10.555
ProMoST 10.101
Dawson 10.657
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.111
Grimsley 10.687
Solomon 10.701
Lehninger 10.687
Nozaki 10.526
DTASelect 10.277
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.862
IPC_peptide 10.716
IPC2_peptide 8.946
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
14501
54
1141
193.3
21.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.31 ± 0.617
1.255 ± 0.168
6.213 ± 0.266
6.675 ± 0.284
3.689 ± 0.18
6.986 ± 0.284
1.862 ± 0.235
6.11 ± 0.201
6.579 ± 0.257
7.103 ± 0.237
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.048 ± 0.201
4.476 ± 0.29
3.448 ± 0.211
4.02 ± 0.284
5.517 ± 0.289
6.848 ± 0.317
5.227 ± 0.345
6.889 ± 0.268
1.386 ± 0.129
3.358 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here