Amino acid dipepetide frequency for Cronobacter phage PhiCS01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.172AlaAla: 11.172 ± 2.281
1.034AlaCys: 1.034 ± 0.322
5.448AlaAsp: 5.448 ± 0.687
6.966AlaGlu: 6.966 ± 0.7
2.69AlaPhe: 2.69 ± 0.443
5.655AlaGly: 5.655 ± 0.614
1.31AlaHis: 1.31 ± 0.281
5.241AlaIle: 5.241 ± 0.586
6.828AlaLys: 6.828 ± 0.977
8.0AlaLeu: 8.0 ± 0.668
2.966AlaMet: 2.966 ± 0.449
3.448AlaAsn: 3.448 ± 0.518
2.138AlaPro: 2.138 ± 0.386
4.345AlaGln: 4.345 ± 0.705
5.172AlaArg: 5.172 ± 0.567
7.379AlaSer: 7.379 ± 1.207
3.862AlaThr: 3.862 ± 0.666
5.793AlaVal: 5.793 ± 0.65
1.241AlaTrp: 1.241 ± 0.285
2.414AlaTyr: 2.414 ± 0.424
0.0AlaXaa: 0.0 ± 0.0
Cys
0.621CysAla: 0.621 ± 0.249
0.276CysCys: 0.276 ± 0.145
1.034CysAsp: 1.034 ± 0.236
1.103CysGlu: 1.103 ± 0.345
0.483CysPhe: 0.483 ± 0.184
1.241CysGly: 1.241 ± 0.39
0.276CysHis: 0.276 ± 0.126
0.759CysIle: 0.759 ± 0.238
0.759CysLys: 0.759 ± 0.262
0.69CysLeu: 0.69 ± 0.192
0.276CysMet: 0.276 ± 0.127
0.621CysAsn: 0.621 ± 0.203
0.345CysPro: 0.345 ± 0.141
0.276CysGln: 0.276 ± 0.128
0.966CysArg: 0.966 ± 0.348
0.621CysSer: 0.621 ± 0.207
0.69CysThr: 0.69 ± 0.246
0.828CysVal: 0.828 ± 0.216
0.345CysTrp: 0.345 ± 0.153
0.345CysTyr: 0.345 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
5.655AspAla: 5.655 ± 0.694
0.414AspCys: 0.414 ± 0.157
4.759AspAsp: 4.759 ± 0.649
4.0AspGlu: 4.0 ± 0.428
2.552AspPhe: 2.552 ± 0.322
6.552AspGly: 6.552 ± 0.757
0.552AspHis: 0.552 ± 0.205
2.828AspIle: 2.828 ± 0.443
3.655AspLys: 3.655 ± 0.442
4.207AspLeu: 4.207 ± 0.573
1.931AspMet: 1.931 ± 0.373
2.483AspAsn: 2.483 ± 0.343
2.414AspPro: 2.414 ± 0.33
2.345AspGln: 2.345 ± 0.502
3.517AspArg: 3.517 ± 0.511
3.793AspSer: 3.793 ± 0.586
2.828AspThr: 2.828 ± 0.52
4.0AspVal: 4.0 ± 0.354
0.966AspTrp: 0.966 ± 0.241
2.69AspTyr: 2.69 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
5.724GluAla: 5.724 ± 0.801
1.034GluCys: 1.034 ± 0.253
2.759GluAsp: 2.759 ± 0.488
4.759GluGlu: 4.759 ± 0.639
2.966GluPhe: 2.966 ± 0.4
2.897GluGly: 2.897 ± 0.46
1.448GluHis: 1.448 ± 0.362
5.862GluIle: 5.862 ± 0.539
3.931GluLys: 3.931 ± 0.482
5.724GluLeu: 5.724 ± 0.649
3.655GluMet: 3.655 ± 0.519
2.138GluAsn: 2.138 ± 0.371
1.931GluPro: 1.931 ± 0.412
3.379GluGln: 3.379 ± 0.707
2.966GluArg: 2.966 ± 0.466
4.483GluSer: 4.483 ± 0.614
3.724GluThr: 3.724 ± 0.677
4.483GluVal: 4.483 ± 0.576
0.69GluTrp: 0.69 ± 0.258
2.207GluTyr: 2.207 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
2.552PheAla: 2.552 ± 0.39
0.552PheCys: 0.552 ± 0.2
2.897PheAsp: 2.897 ± 0.553
2.345PheGlu: 2.345 ± 0.343
1.31PhePhe: 1.31 ± 0.351
3.034PheGly: 3.034 ± 0.447
0.621PheHis: 0.621 ± 0.186
1.724PheIle: 1.724 ± 0.333
2.0PheLys: 2.0 ± 0.357
2.207PheLeu: 2.207 ± 0.311
1.034PheMet: 1.034 ± 0.23
2.759PheAsn: 2.759 ± 0.447
1.379PhePro: 1.379 ± 0.309
1.31PheGln: 1.31 ± 0.275
1.931PheArg: 1.931 ± 0.342
2.414PheSer: 2.414 ± 0.448
2.414PheThr: 2.414 ± 0.401
2.621PheVal: 2.621 ± 0.336
0.69PheTrp: 0.69 ± 0.191
1.103PheTyr: 1.103 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
5.655GlyAla: 5.655 ± 0.628
0.897GlyCys: 0.897 ± 0.213
5.103GlyAsp: 5.103 ± 0.624
4.069GlyGlu: 4.069 ± 0.511
2.483GlyPhe: 2.483 ± 0.452
5.517GlyGly: 5.517 ± 0.902
1.448GlyHis: 1.448 ± 0.429
3.862GlyIle: 3.862 ± 0.525
6.414GlyLys: 6.414 ± 0.571
4.69GlyLeu: 4.69 ± 0.443
2.483GlyMet: 2.483 ± 0.422
2.828GlyAsn: 2.828 ± 0.462
1.034GlyPro: 1.034 ± 0.308
1.655GlyGln: 1.655 ± 0.337
3.724GlyArg: 3.724 ± 0.46
4.897GlySer: 4.897 ± 0.642
2.897GlyThr: 2.897 ± 0.677
5.724GlyVal: 5.724 ± 0.635
1.448GlyTrp: 1.448 ± 0.319
3.034GlyTyr: 3.034 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.517HisAla: 1.517 ± 0.382
0.276HisCys: 0.276 ± 0.126
1.586HisAsp: 1.586 ± 0.367
1.172HisGlu: 1.172 ± 0.294
0.69HisPhe: 0.69 ± 0.211
1.931HisGly: 1.931 ± 0.525
0.621HisHis: 0.621 ± 0.238
0.966HisIle: 0.966 ± 0.218
0.966HisLys: 0.966 ± 0.286
1.586HisLeu: 1.586 ± 0.422
0.345HisMet: 0.345 ± 0.164
0.552HisAsn: 0.552 ± 0.203
1.103HisPro: 1.103 ± 0.268
0.621HisGln: 0.621 ± 0.186
1.103HisArg: 1.103 ± 0.322
0.828HisSer: 0.828 ± 0.198
0.759HisThr: 0.759 ± 0.205
1.103HisVal: 1.103 ± 0.321
0.207HisTrp: 0.207 ± 0.122
0.69HisTyr: 0.69 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.862IleAla: 5.862 ± 0.464
1.241IleCys: 1.241 ± 0.342
3.862IleAsp: 3.862 ± 0.528
4.0IleGlu: 4.0 ± 0.489
2.276IlePhe: 2.276 ± 0.458
3.517IleGly: 3.517 ± 0.401
1.31IleHis: 1.31 ± 0.288
3.586IleIle: 3.586 ± 0.532
4.0IleLys: 4.0 ± 0.397
3.034IleLeu: 3.034 ± 0.508
1.793IleMet: 1.793 ± 0.417
3.379IleAsn: 3.379 ± 0.604
2.276IlePro: 2.276 ± 0.383
2.276IleGln: 2.276 ± 0.496
3.172IleArg: 3.172 ± 0.476
4.759IleSer: 4.759 ± 0.636
4.207IleThr: 4.207 ± 0.659
4.069IleVal: 4.069 ± 0.515
0.897IleTrp: 0.897 ± 0.244
1.586IleTyr: 1.586 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
5.241LysAla: 5.241 ± 0.676
0.897LysCys: 0.897 ± 0.283
3.931LysAsp: 3.931 ± 0.551
4.483LysGlu: 4.483 ± 0.527
2.483LysPhe: 2.483 ± 0.424
3.931LysGly: 3.931 ± 0.382
1.517LysHis: 1.517 ± 0.386
3.724LysIle: 3.724 ± 0.419
5.034LysLys: 5.034 ± 0.758
5.103LysLeu: 5.103 ± 0.767
2.345LysMet: 2.345 ± 0.472
3.517LysAsn: 3.517 ± 0.501
2.897LysPro: 2.897 ± 0.447
2.069LysGln: 2.069 ± 0.442
3.034LysArg: 3.034 ± 0.542
4.276LysSer: 4.276 ± 0.508
4.276LysThr: 4.276 ± 0.714
3.862LysVal: 3.862 ± 0.674
1.103LysTrp: 1.103 ± 0.267
2.069LysTyr: 2.069 ± 0.388
0.0LysXaa: 0.0 ± 0.0
Leu
6.828LeuAla: 6.828 ± 0.858
0.69LeuCys: 0.69 ± 0.18
3.586LeuAsp: 3.586 ± 0.57
4.0LeuGlu: 4.0 ± 0.514
2.414LeuPhe: 2.414 ± 0.425
5.241LeuGly: 5.241 ± 0.673
1.517LeuHis: 1.517 ± 0.46
4.138LeuIle: 4.138 ± 0.5
4.897LeuLys: 4.897 ± 0.496
4.414LeuLeu: 4.414 ± 0.565
2.138LeuMet: 2.138 ± 0.406
2.966LeuAsn: 2.966 ± 0.423
2.966LeuPro: 2.966 ± 0.503
3.172LeuGln: 3.172 ± 0.644
4.414LeuArg: 4.414 ± 0.59
5.862LeuSer: 5.862 ± 0.543
3.931LeuThr: 3.931 ± 0.465
4.69LeuVal: 4.69 ± 0.448
0.897LeuTrp: 0.897 ± 0.246
2.276LeuTyr: 2.276 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
3.034MetAla: 3.034 ± 0.465
0.621MetCys: 0.621 ± 0.254
1.172MetAsp: 1.172 ± 0.273
1.448MetGlu: 1.448 ± 0.281
0.828MetPhe: 0.828 ± 0.223
1.034MetGly: 1.034 ± 0.265
0.483MetHis: 0.483 ± 0.179
3.034MetIle: 3.034 ± 0.44
2.69MetLys: 2.69 ± 0.524
2.828MetLeu: 2.828 ± 0.559
1.034MetMet: 1.034 ± 0.267
1.172MetAsn: 1.172 ± 0.261
0.897MetPro: 0.897 ± 0.239
1.448MetGln: 1.448 ± 0.358
2.345MetArg: 2.345 ± 0.329
1.793MetSer: 1.793 ± 0.276
1.655MetThr: 1.655 ± 0.288
2.0MetVal: 2.0 ± 0.368
0.207MetTrp: 0.207 ± 0.127
0.759MetTyr: 0.759 ± 0.252
0.0MetXaa: 0.0 ± 0.0
Asn
4.828AsnAla: 4.828 ± 0.91
0.621AsnCys: 0.621 ± 0.247
2.828AsnAsp: 2.828 ± 0.379
2.345AsnGlu: 2.345 ± 0.339
1.517AsnPhe: 1.517 ± 0.305
4.483AsnGly: 4.483 ± 0.571
0.897AsnHis: 0.897 ± 0.277
2.483AsnIle: 2.483 ± 0.412
2.0AsnLys: 2.0 ± 0.379
3.448AsnLeu: 3.448 ± 0.383
0.897AsnMet: 0.897 ± 0.284
1.862AsnAsn: 1.862 ± 0.428
2.138AsnPro: 2.138 ± 0.488
1.931AsnGln: 1.931 ± 0.329
2.207AsnArg: 2.207 ± 0.394
2.483AsnSer: 2.483 ± 0.552
2.069AsnThr: 2.069 ± 0.291
3.103AsnVal: 3.103 ± 0.597
0.759AsnTrp: 0.759 ± 0.189
1.862AsnTyr: 1.862 ± 0.369
0.0AsnXaa: 0.0 ± 0.0
Pro
2.966ProAla: 2.966 ± 0.425
0.483ProCys: 0.483 ± 0.238
3.655ProAsp: 3.655 ± 0.522
3.172ProGlu: 3.172 ± 0.646
1.379ProPhe: 1.379 ± 0.239
2.138ProGly: 2.138 ± 0.383
0.552ProHis: 0.552 ± 0.189
1.31ProIle: 1.31 ± 0.305
1.517ProLys: 1.517 ± 0.315
1.931ProLeu: 1.931 ± 0.395
0.966ProMet: 0.966 ± 0.275
1.448ProAsn: 1.448 ± 0.374
1.034ProPro: 1.034 ± 0.245
1.379ProGln: 1.379 ± 0.326
1.931ProArg: 1.931 ± 0.352
2.138ProSer: 2.138 ± 0.359
1.31ProThr: 1.31 ± 0.349
3.31ProVal: 3.31 ± 0.496
0.414ProTrp: 0.414 ± 0.167
1.448ProTyr: 1.448 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
4.069GlnAla: 4.069 ± 0.565
0.483GlnCys: 0.483 ± 0.222
1.793GlnAsp: 1.793 ± 0.377
2.483GlnGlu: 2.483 ± 0.544
1.31GlnPhe: 1.31 ± 0.29
2.0GlnGly: 2.0 ± 0.37
0.828GlnHis: 0.828 ± 0.265
3.241GlnIle: 3.241 ± 0.459
2.552GlnLys: 2.552 ± 0.58
2.552GlnLeu: 2.552 ± 0.49
1.103GlnMet: 1.103 ± 0.287
1.724GlnAsn: 1.724 ± 0.313
0.897GlnPro: 0.897 ± 0.321
2.621GlnGln: 2.621 ± 0.617
1.793GlnArg: 1.793 ± 0.356
2.69GlnSer: 2.69 ± 0.415
1.931GlnThr: 1.931 ± 0.315
3.862GlnVal: 3.862 ± 0.413
0.552GlnTrp: 0.552 ± 0.186
1.724GlnTyr: 1.724 ± 0.303
0.0GlnXaa: 0.0 ± 0.0
Arg
5.172ArgAla: 5.172 ± 0.513
0.621ArgCys: 0.621 ± 0.287
2.828ArgAsp: 2.828 ± 0.445
3.517ArgGlu: 3.517 ± 0.561
2.414ArgPhe: 2.414 ± 0.421
3.379ArgGly: 3.379 ± 0.396
1.103ArgHis: 1.103 ± 0.292
4.276ArgIle: 4.276 ± 0.434
4.0ArgLys: 4.0 ± 0.599
4.069ArgLeu: 4.069 ± 0.652
2.0ArgMet: 2.0 ± 0.355
1.931ArgAsn: 1.931 ± 0.473
2.414ArgPro: 2.414 ± 0.552
2.207ArgGln: 2.207 ± 0.538
3.517ArgArg: 3.517 ± 0.509
3.241ArgSer: 3.241 ± 0.487
2.345ArgThr: 2.345 ± 0.351
4.0ArgVal: 4.0 ± 0.905
0.414ArgTrp: 0.414 ± 0.179
1.724ArgTyr: 1.724 ± 0.355
0.0ArgXaa: 0.0 ± 0.0
Ser
8.621SerAla: 8.621 ± 1.515
0.552SerCys: 0.552 ± 0.18
3.931SerAsp: 3.931 ± 0.477
5.862SerGlu: 5.862 ± 0.65
2.69SerPhe: 2.69 ± 0.469
5.793SerGly: 5.793 ± 0.598
1.172SerHis: 1.172 ± 0.3
4.0SerIle: 4.0 ± 0.611
4.207SerLys: 4.207 ± 0.641
5.034SerLeu: 5.034 ± 0.543
1.793SerMet: 1.793 ± 0.291
2.69SerAsn: 2.69 ± 0.61
1.793SerPro: 1.793 ± 0.27
2.621SerGln: 2.621 ± 0.365
3.793SerArg: 3.793 ± 0.541
3.862SerSer: 3.862 ± 0.607
2.621SerThr: 2.621 ± 0.377
4.483SerVal: 4.483 ± 0.624
0.828SerTrp: 0.828 ± 0.223
2.069SerTyr: 2.069 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
4.828ThrAla: 4.828 ± 0.686
0.483ThrCys: 0.483 ± 0.214
2.966ThrAsp: 2.966 ± 0.396
2.759ThrGlu: 2.759 ± 0.525
2.138ThrPhe: 2.138 ± 0.412
4.69ThrGly: 4.69 ± 0.444
0.414ThrHis: 0.414 ± 0.158
3.103ThrIle: 3.103 ± 0.468
2.759ThrLys: 2.759 ± 0.493
3.517ThrLeu: 3.517 ± 0.45
0.828ThrMet: 0.828 ± 0.268
3.448ThrAsn: 3.448 ± 0.662
2.69ThrPro: 2.69 ± 0.347
2.207ThrGln: 2.207 ± 0.481
1.862ThrArg: 1.862 ± 0.335
4.414ThrSer: 4.414 ± 1.131
2.897ThrThr: 2.897 ± 0.468
3.448ThrVal: 3.448 ± 0.502
0.759ThrTrp: 0.759 ± 0.247
2.069ThrTyr: 2.069 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
5.517ValAla: 5.517 ± 0.737
0.69ValCys: 0.69 ± 0.236
4.483ValAsp: 4.483 ± 0.52
5.103ValGlu: 5.103 ± 0.643
2.414ValPhe: 2.414 ± 0.437
3.448ValGly: 3.448 ± 0.415
1.586ValHis: 1.586 ± 0.319
4.828ValIle: 4.828 ± 0.58
4.966ValLys: 4.966 ± 0.615
4.0ValLeu: 4.0 ± 0.474
1.655ValMet: 1.655 ± 0.345
3.379ValAsn: 3.379 ± 0.484
2.621ValPro: 2.621 ± 0.438
2.483ValGln: 2.483 ± 0.431
4.276ValArg: 4.276 ± 0.597
5.172ValSer: 5.172 ± 0.701
4.897ValThr: 4.897 ± 0.715
4.414ValVal: 4.414 ± 0.499
0.897ValTrp: 0.897 ± 0.231
2.069ValTyr: 2.069 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
0.828TrpAla: 0.828 ± 0.252
0.138TrpCys: 0.138 ± 0.091
0.966TrpAsp: 0.966 ± 0.267
0.759TrpGlu: 0.759 ± 0.249
0.552TrpPhe: 0.552 ± 0.168
0.828TrpGly: 0.828 ± 0.222
0.552TrpHis: 0.552 ± 0.18
0.897TrpIle: 0.897 ± 0.272
0.966TrpLys: 0.966 ± 0.22
1.517TrpLeu: 1.517 ± 0.333
0.483TrpMet: 0.483 ± 0.169
0.897TrpAsn: 0.897 ± 0.233
0.345TrpPro: 0.345 ± 0.18
0.069TrpGln: 0.069 ± 0.067
1.034TrpArg: 1.034 ± 0.254
0.966TrpSer: 0.966 ± 0.429
0.621TrpThr: 0.621 ± 0.25
0.69TrpVal: 0.69 ± 0.224
0.207TrpTrp: 0.207 ± 0.105
0.552TrpTyr: 0.552 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.414TyrAla: 2.414 ± 0.433
0.552TyrCys: 0.552 ± 0.181
2.552TyrAsp: 2.552 ± 0.478
2.414TyrGlu: 2.414 ± 0.387
1.31TyrPhe: 1.31 ± 0.302
2.552TyrGly: 2.552 ± 0.417
0.414TyrHis: 0.414 ± 0.145
1.241TyrIle: 1.241 ± 0.289
1.655TyrLys: 1.655 ± 0.347
2.483TyrLeu: 2.483 ± 0.329
0.69TyrMet: 0.69 ± 0.224
1.517TyrAsn: 1.517 ± 0.314
1.172TyrPro: 1.172 ± 0.271
1.793TyrGln: 1.793 ± 0.34
2.414TyrArg: 2.414 ± 0.378
2.414TyrSer: 2.414 ± 0.402
2.345TyrThr: 2.345 ± 0.362
2.414TyrVal: 2.414 ± 0.474
0.345TyrTrp: 0.345 ± 0.132
0.897TyrTyr: 0.897 ± 0.205
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14501 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski