Butyrivibrio sp. CB08

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9SN74|A0A3A9SN74_9FIRM PaaI family thioesterase OS=Butyrivibrio sp. CB08 OX=2364879 GN=D6855_06240 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.32KK3 pKa = 10.03SIAMIMTGLMVLSLTACGQAAPAEE27 pKa = 4.3APAAEE32 pKa = 4.43EE33 pKa = 4.41TEE35 pKa = 4.18QAAAEE40 pKa = 4.04ASVEE44 pKa = 4.21STEE47 pKa = 4.97IANPWRR53 pKa = 11.84DD54 pKa = 3.19ASEE57 pKa = 4.22EE58 pKa = 3.94EE59 pKa = 4.38CRR61 pKa = 11.84QLVGNGFSAPEE72 pKa = 3.81GATNVKK78 pKa = 9.05WSVMDD83 pKa = 3.33SGDD86 pKa = 3.79PYY88 pKa = 10.72RR89 pKa = 11.84QLVQMTFDD97 pKa = 4.88LDD99 pKa = 4.18DD100 pKa = 4.21KK101 pKa = 11.59SFTARR106 pKa = 11.84QQATGDD112 pKa = 3.6NAEE115 pKa = 5.15DD116 pKa = 3.65LSGMYY121 pKa = 10.19FDD123 pKa = 4.32WTVEE127 pKa = 4.19DD128 pKa = 5.91DD129 pKa = 3.62VTLANWAGGNMAGKK143 pKa = 9.96CKK145 pKa = 10.38RR146 pKa = 11.84FIGDD150 pKa = 3.76TEE152 pKa = 4.34WVDD155 pKa = 3.03VCTWYY160 pKa = 10.27DD161 pKa = 3.46VEE163 pKa = 4.65TGDD166 pKa = 5.18SYY168 pKa = 12.07SLSTTAKK175 pKa = 10.37DD176 pKa = 3.68LDD178 pKa = 4.05GFDD181 pKa = 3.26IQAIAEE187 pKa = 4.62AIYY190 pKa = 10.64DD191 pKa = 3.76PNKK194 pKa = 10.3QFGANAPEE202 pKa = 4.01EE203 pKa = 4.26SGEE206 pKa = 4.06LQEE209 pKa = 5.34HH210 pKa = 6.28IPMVNLDD217 pKa = 3.58GCDD220 pKa = 3.17TFTQIVDD227 pKa = 3.75KK228 pKa = 11.04LPAGSGYY235 pKa = 9.22TNIKK239 pKa = 10.36LGDD242 pKa = 3.66TDD244 pKa = 3.93VLLVASWTFDD254 pKa = 3.5DD255 pKa = 6.09LEE257 pKa = 4.25GHH259 pKa = 6.24NAAIDD264 pKa = 3.8AEE266 pKa = 4.8VYY268 pKa = 10.53SYY270 pKa = 11.02TDD272 pKa = 2.62SGTPQYY278 pKa = 11.5AGFVEE283 pKa = 4.61AAGTAYY289 pKa = 9.86PLAVNNDD296 pKa = 3.22KK297 pKa = 11.25LYY299 pKa = 10.86VGGNHH304 pKa = 5.64FMKK307 pKa = 10.47VYY309 pKa = 8.17TSSYY313 pKa = 10.8GFPCWDD319 pKa = 3.01EE320 pKa = 4.21FAWEE324 pKa = 4.19EE325 pKa = 4.28FDD327 pKa = 3.72TDD329 pKa = 3.89GKK331 pKa = 8.95ATYY334 pKa = 10.03YY335 pKa = 10.38YY336 pKa = 10.6RR337 pKa = 11.84SDD339 pKa = 3.18LHH341 pKa = 7.6DD342 pKa = 3.81IADD345 pKa = 3.65VGADD349 pKa = 3.51EE350 pKa = 5.58NGVLKK355 pKa = 10.87DD356 pKa = 3.5NSVLSRR362 pKa = 11.84LYY364 pKa = 10.5DD365 pKa = 3.25EE366 pKa = 5.39CFSGEE371 pKa = 3.97ILNFDD376 pKa = 4.11VIKK379 pKa = 10.95

Molecular weight: 41.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9SJX6|A0A3A9SJX6_9FIRM ArsC family transcriptional regulator OS=Butyrivibrio sp. CB08 OX=2364879 GN=D6855_09760 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3241

0

3241

1078809

21

3130

332.9

37.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.051

1.349 ± 0.019

6.529 ± 0.035

7.281 ± 0.05

4.369 ± 0.031

7.12 ± 0.04

1.616 ± 0.018

7.56 ± 0.044

6.877 ± 0.04

8.504 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.112 ± 0.022

4.435 ± 0.027

3.186 ± 0.023

2.774 ± 0.022

4.097 ± 0.038

6.105 ± 0.032

5.362 ± 0.036

7.013 ± 0.035

0.892 ± 0.016

4.28 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski