Amino acid dipepetide frequency for Butyrivibrio sp. CB08

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.037AlaAla: 7.037 ± 0.12	0.999AlaCys: 0.999 ± 0.031	4.933AlaAsp: 4.933 ± 0.075	5.183AlaGlu: 5.183 ± 0.078	3.166AlaPhe: 3.166 ± 0.056	5.89AlaGly: 5.89 ± 0.084	1.034AlaHis: 1.034 ± 0.031	5.534AlaIle: 5.534 ± 0.079	4.882AlaLys: 4.882 ± 0.077	7.041AlaLeu: 7.041 ± 0.097	2.399AlaMet: 2.399 ± 0.052	2.738AlaAsn: 2.738 ± 0.066	2.117AlaPro: 2.117 ± 0.056	2.225AlaGln: 2.225 ± 0.054	2.912AlaArg: 2.912 ± 0.053	4.358AlaSer: 4.358 ± 0.069	3.793AlaThr: 3.793 ± 0.072	5.853AlaVal: 5.853 ± 0.085	0.601AlaTrp: 0.601 ± 0.023	2.699AlaTyr: 2.699 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.97CysAla: 0.97 ± 0.034	0.241CysCys: 0.241 ± 0.016	0.934CysAsp: 0.934 ± 0.034	0.833CysGlu: 0.833 ± 0.029	0.632CysPhe: 0.632 ± 0.023	1.349CysGly: 1.349 ± 0.042	0.273CysHis: 0.273 ± 0.018	1.019CysIle: 1.019 ± 0.029	0.778CysLys: 0.778 ± 0.029	1.026CysLeu: 1.026 ± 0.032	0.396CysMet: 0.396 ± 0.021	0.564CysAsn: 0.564 ± 0.024	0.544CysPro: 0.544 ± 0.026	0.345CysGln: 0.345 ± 0.019	0.59CysArg: 0.59 ± 0.026	0.787CysSer: 0.787 ± 0.027	0.593CysThr: 0.593 ± 0.025	0.938CysVal: 0.938 ± 0.03	0.118CysTrp: 0.118 ± 0.011	0.565CysTyr: 0.565 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.728AspAla: 4.728 ± 0.08	0.722AspCys: 0.722 ± 0.028	4.042AspAsp: 4.042 ± 0.083	5.636AspGlu: 5.636 ± 0.087	3.004AspPhe: 3.004 ± 0.06	4.94AspGly: 4.94 ± 0.089	1.015AspHis: 1.015 ± 0.033	5.192AspIle: 5.192 ± 0.073	4.643AspLys: 4.643 ± 0.073	4.866AspLeu: 4.866 ± 0.067	2.084AspMet: 2.084 ± 0.044	2.836AspAsn: 2.836 ± 0.058	2.189AspPro: 2.189 ± 0.047	1.535AspGln: 1.535 ± 0.04	2.673AspArg: 2.673 ± 0.048	3.597AspSer: 3.597 ± 0.055	3.305AspThr: 3.305 ± 0.056	4.418AspVal: 4.418 ± 0.064	0.571AspTrp: 0.571 ± 0.021	3.293AspTyr: 3.293 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	5.634GluAla: 5.634 ± 0.087	0.759GluCys: 0.759 ± 0.026	4.724GluAsp: 4.724 ± 0.078	6.242GluGlu: 6.242 ± 0.127	2.78GluPhe: 2.78 ± 0.049	4.593GluGly: 4.593 ± 0.067	1.298GluHis: 1.298 ± 0.035	5.428GluIle: 5.428 ± 0.075	6.065GluLys: 6.065 ± 0.093	6.496GluLeu: 6.496 ± 0.091	2.35GluMet: 2.35 ± 0.046	3.761GluAsn: 3.761 ± 0.068	1.899GluPro: 1.899 ± 0.053	2.292GluGln: 2.292 ± 0.048	2.962GluArg: 2.962 ± 0.057	3.63GluSer: 3.63 ± 0.061	3.521GluThr: 3.521 ± 0.052	4.581GluVal: 4.581 ± 0.066	0.59GluTrp: 0.59 ± 0.023	3.203GluTyr: 3.203 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	3.157PheAla: 3.157 ± 0.057	0.71PheCys: 0.71 ± 0.027	3.175PheAsp: 3.175 ± 0.05	2.925PheGlu: 2.925 ± 0.055	2.179PhePhe: 2.179 ± 0.055	3.317PheGly: 3.317 ± 0.062	0.698PheHis: 0.698 ± 0.025	3.154PheIle: 3.154 ± 0.058	2.532PheLys: 2.532 ± 0.043	3.829PheLeu: 3.829 ± 0.078	1.3PheMet: 1.3 ± 0.045	1.769PheAsn: 1.769 ± 0.044	1.398PhePro: 1.398 ± 0.035	0.975PheGln: 0.975 ± 0.031	1.618PheArg: 1.618 ± 0.039	2.879PheSer: 2.879 ± 0.062	2.513PheThr: 2.513 ± 0.052	3.241PheVal: 3.241 ± 0.055	0.48PheTrp: 0.48 ± 0.022	1.838PheTyr: 1.838 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.228GlyAla: 5.228 ± 0.079	1.188GlyCys: 1.188 ± 0.038	4.306GlyAsp: 4.306 ± 0.07	4.507GlyGlu: 4.507 ± 0.06	3.319GlyPhe: 3.319 ± 0.06	4.781GlyGly: 4.781 ± 0.091	1.223GlyHis: 1.223 ± 0.041	6.003GlyIle: 6.003 ± 0.073	5.437GlyLys: 5.437 ± 0.079	5.825GlyLeu: 5.825 ± 0.08	2.413GlyMet: 2.413 ± 0.046	3.265GlyAsn: 3.265 ± 0.066	1.419GlyPro: 1.419 ± 0.036	1.976GlyGln: 1.976 ± 0.044	2.957GlyArg: 2.957 ± 0.057	4.312GlySer: 4.312 ± 0.062	4.053GlyThr: 4.053 ± 0.073	4.92GlyVal: 4.92 ± 0.069	0.671GlyTrp: 0.671 ± 0.025	3.394GlyTyr: 3.394 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.003HisAla: 1.003 ± 0.035	0.252HisCys: 0.252 ± 0.017	1.02HisAsp: 1.02 ± 0.039	1.145HisGlu: 1.145 ± 0.036	0.8HisPhe: 0.8 ± 0.031	1.182HisGly: 1.182 ± 0.036	0.389HisHis: 0.389 ± 0.028	1.186HisIle: 1.186 ± 0.033	0.941HisLys: 0.941 ± 0.031	1.369HisLeu: 1.369 ± 0.037	0.544HisMet: 0.544 ± 0.022	0.722HisAsn: 0.722 ± 0.027	0.779HisPro: 0.779 ± 0.027	0.428HisGln: 0.428 ± 0.018	0.686HisArg: 0.686 ± 0.027	0.932HisSer: 0.932 ± 0.027	0.817HisThr: 0.817 ± 0.025	1.092HisVal: 1.092 ± 0.031	0.133HisTrp: 0.133 ± 0.012	0.737HisTyr: 0.737 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.941IleAla: 5.941 ± 0.087	1.222IleCys: 1.222 ± 0.035	4.962IleAsp: 4.962 ± 0.064	4.967IleGlu: 4.967 ± 0.07	3.017IlePhe: 3.017 ± 0.06	5.142IleGly: 5.142 ± 0.073	1.206IleHis: 1.206 ± 0.032	5.658IleIle: 5.658 ± 0.094	4.687IleLys: 4.687 ± 0.077	6.509IleLeu: 6.509 ± 0.108	2.228IleMet: 2.228 ± 0.054	3.259IleAsn: 3.259 ± 0.053	3.089IlePro: 3.089 ± 0.055	1.816IleGln: 1.816 ± 0.042	3.214IleArg: 3.214 ± 0.058	5.216IleSer: 5.216 ± 0.07	4.536IleThr: 4.536 ± 0.067	5.286IleVal: 5.286 ± 0.084	0.644IleTrp: 0.644 ± 0.026	3.003IleTyr: 3.003 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.428LysAla: 5.428 ± 0.084	0.726LysCys: 0.726 ± 0.03	4.54LysAsp: 4.54 ± 0.069	6.517LysGlu: 6.517 ± 0.077	2.165LysPhe: 2.165 ± 0.045	4.321LysGly: 4.321 ± 0.066	1.004LysHis: 1.004 ± 0.031	4.565LysIle: 4.565 ± 0.065	6.091LysLys: 6.091 ± 0.085	5.459LysLeu: 5.459 ± 0.071	2.164LysMet: 2.164 ± 0.048	3.502LysAsn: 3.502 ± 0.064	1.957LysPro: 1.957 ± 0.043	1.947LysGln: 1.947 ± 0.047	3.143LysArg: 3.143 ± 0.062	3.567LysSer: 3.567 ± 0.054	3.602LysThr: 3.602 ± 0.062	4.445LysVal: 4.445 ± 0.074	0.675LysTrp: 0.675 ± 0.026	2.949LysTyr: 2.949 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	6.23LeuAla: 6.23 ± 0.081	1.263LeuCys: 1.263 ± 0.037	5.341LeuAsp: 5.341 ± 0.071	5.537LeuGlu: 5.537 ± 0.081	3.991LeuPhe: 3.991 ± 0.082	5.77LeuGly: 5.77 ± 0.081	1.293LeuHis: 1.293 ± 0.037	6.199LeuIle: 6.199 ± 0.088	5.906LeuLys: 5.906 ± 0.075	7.583LeuLeu: 7.583 ± 0.108	2.621LeuMet: 2.621 ± 0.058	3.55LeuAsn: 3.55 ± 0.057	3.077LeuPro: 3.077 ± 0.054	2.379LeuGln: 2.379 ± 0.052	3.413LeuArg: 3.413 ± 0.062	6.479LeuSer: 6.479 ± 0.085	4.753LeuThr: 4.753 ± 0.069	5.607LeuVal: 5.607 ± 0.076	0.692LeuTrp: 0.692 ± 0.029	3.355LeuTyr: 3.355 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.557MetAla: 2.557 ± 0.061	0.37MetCys: 0.37 ± 0.019	2.001MetAsp: 2.001 ± 0.045	2.075MetGlu: 2.075 ± 0.05	1.115MetPhe: 1.115 ± 0.033	2.096MetGly: 2.096 ± 0.049	0.521MetHis: 0.521 ± 0.021	2.329MetIle: 2.329 ± 0.058	2.243MetLys: 2.243 ± 0.047	2.627MetLeu: 2.627 ± 0.051	1.033MetMet: 1.033 ± 0.037	1.662MetAsn: 1.662 ± 0.042	1.134MetPro: 1.134 ± 0.035	1.011MetGln: 1.011 ± 0.029	1.236MetArg: 1.236 ± 0.031	2.059MetSer: 2.059 ± 0.044	1.76MetThr: 1.76 ± 0.039	1.999MetVal: 1.999 ± 0.045	0.243MetTrp: 0.243 ± 0.014	1.051MetTyr: 1.051 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.229AsnAla: 3.229 ± 0.063	0.564AsnCys: 0.564 ± 0.023	2.519AsnAsp: 2.519 ± 0.045	2.787AsnGlu: 2.787 ± 0.055	1.662AsnPhe: 1.662 ± 0.041	3.551AsnGly: 3.551 ± 0.068	0.767AsnHis: 0.767 ± 0.027	3.566AsnIle: 3.566 ± 0.059	2.866AsnLys: 2.866 ± 0.057	3.671AsnLeu: 3.671 ± 0.059	1.476AsnMet: 1.476 ± 0.037	2.101AsnAsn: 2.101 ± 0.055	1.85AsnPro: 1.85 ± 0.041	1.186AsnGln: 1.186 ± 0.032	1.846AsnArg: 1.846 ± 0.042	2.539AsnSer: 2.539 ± 0.049	2.468AsnThr: 2.468 ± 0.049	3.243AsnVal: 3.243 ± 0.056	0.447AsnTrp: 0.447 ± 0.018	2.016AsnTyr: 2.016 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.392ProAla: 2.392 ± 0.069	0.39ProCys: 0.39 ± 0.022	2.533ProAsp: 2.533 ± 0.047	3.152ProGlu: 3.152 ± 0.061	1.537ProPhe: 1.537 ± 0.042	2.252ProGly: 2.252 ± 0.05	0.509ProHis: 0.509 ± 0.027	2.188ProIle: 2.188 ± 0.046	1.866ProLys: 1.866 ± 0.044	2.484ProLeu: 2.484 ± 0.056	0.882ProMet: 0.882 ± 0.03	1.162ProAsn: 1.162 ± 0.033	0.59ProPro: 0.59 ± 0.029	0.94ProGln: 0.94 ± 0.032	0.966ProArg: 0.966 ± 0.038	1.91ProSer: 1.91 ± 0.045	1.526ProThr: 1.526 ± 0.039	2.833ProVal: 2.833 ± 0.056	0.308ProTrp: 0.308 ± 0.017	1.44ProTyr: 1.44 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.061GlnAla: 2.061 ± 0.046	0.293GlnCys: 0.293 ± 0.018	1.573GlnAsp: 1.573 ± 0.037	2.137GlnGlu: 2.137 ± 0.045	1.161GlnPhe: 1.161 ± 0.032	1.86GlnGly: 1.86 ± 0.043	0.399GlnHis: 0.399 ± 0.017	2.323GlnIle: 2.323 ± 0.045	2.113GlnLys: 2.113 ± 0.049	2.384GlnLeu: 2.384 ± 0.044	1.017GlnMet: 1.017 ± 0.031	1.321GlnAsn: 1.321 ± 0.036	0.754GlnPro: 0.754 ± 0.027	1.032GlnGln: 1.032 ± 0.033	1.18GlnArg: 1.18 ± 0.037	1.493GlnSer: 1.493 ± 0.04	1.406GlnThr: 1.406 ± 0.037	1.717GlnVal: 1.717 ± 0.039	0.228GlnTrp: 0.228 ± 0.014	1.291GlnTyr: 1.291 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.752ArgAla: 2.752 ± 0.043	0.506ArgCys: 0.506 ± 0.022	2.658ArgAsp: 2.658 ± 0.051	3.074ArgGlu: 3.074 ± 0.047	1.883ArgPhe: 1.883 ± 0.041	2.417ArgGly: 2.417 ± 0.056	0.747ArgHis: 0.747 ± 0.032	3.37ArgIle: 3.37 ± 0.062	3.049ArgLys: 3.049 ± 0.066	3.503ArgLeu: 3.503 ± 0.065	1.377ArgMet: 1.377 ± 0.035	1.999ArgAsn: 1.999 ± 0.046	1.269ArgPro: 1.269 ± 0.036	1.403ArgGln: 1.403 ± 0.042	1.928ArgArg: 1.928 ± 0.046	2.125ArgSer: 2.125 ± 0.047	2.069ArgThr: 2.069 ± 0.04	2.623ArgVal: 2.623 ± 0.054	0.38ArgTrp: 0.38 ± 0.022	1.838ArgTyr: 1.838 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	4.359SerAla: 4.359 ± 0.071	0.783SerCys: 0.783 ± 0.027	4.322SerAsp: 4.322 ± 0.084	4.437SerGlu: 4.437 ± 0.07	3.287SerPhe: 3.287 ± 0.062	5.041SerGly: 5.041 ± 0.077	0.96SerHis: 0.96 ± 0.034	4.381SerIle: 4.381 ± 0.071	3.813SerLys: 3.813 ± 0.062	5.137SerLeu: 5.137 ± 0.071	1.821SerMet: 1.821 ± 0.039	2.393SerAsn: 2.393 ± 0.049	1.647SerPro: 1.647 ± 0.043	1.666SerGln: 1.666 ± 0.038	2.512SerArg: 2.512 ± 0.049	4.004SerSer: 4.004 ± 0.08	2.915SerThr: 2.915 ± 0.055	4.395SerVal: 4.395 ± 0.065	0.545SerTrp: 0.545 ± 0.025	2.628SerTyr: 2.628 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	4.215ThrAla: 4.215 ± 0.073	0.655ThrCys: 0.655 ± 0.024	3.659ThrAsp: 3.659 ± 0.062	3.614ThrGlu: 3.614 ± 0.064	2.381ThrPhe: 2.381 ± 0.048	4.566ThrGly: 4.566 ± 0.073	0.785ThrHis: 0.785 ± 0.025	4.129ThrIle: 4.129 ± 0.071	3.051ThrLys: 3.051 ± 0.06	4.414ThrLeu: 4.414 ± 0.069	1.44ThrMet: 1.44 ± 0.042	2.077ThrAsn: 2.077 ± 0.045	2.032ThrPro: 2.032 ± 0.051	1.426ThrGln: 1.426 ± 0.038	2.069ThrArg: 2.069 ± 0.044	3.111ThrSer: 3.111 ± 0.047	2.988ThrThr: 2.988 ± 0.063	4.267ThrVal: 4.267 ± 0.068	0.498ThrTrp: 0.498 ± 0.021	2.245ThrTyr: 2.245 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	5.162ValAla: 5.162 ± 0.081	1.106ValCys: 1.106 ± 0.034	4.417ValAsp: 4.417 ± 0.07	4.339ValGlu: 4.339 ± 0.073	3.239ValPhe: 3.239 ± 0.058	4.369ValGly: 4.369 ± 0.067	1.058ValHis: 1.058 ± 0.031	5.61ValIle: 5.61 ± 0.076	4.623ValLys: 4.623 ± 0.07	6.429ValLeu: 6.429 ± 0.093	2.087ValMet: 2.087 ± 0.046	3.043ValAsn: 3.043 ± 0.058	2.54ValPro: 2.54 ± 0.047	1.609ValGln: 1.609 ± 0.038	2.767ValArg: 2.767 ± 0.06	4.905ValSer: 4.905 ± 0.078	4.235ValThr: 4.235 ± 0.085	5.261ValVal: 5.261 ± 0.077	0.592ValTrp: 0.592 ± 0.021	2.736ValTyr: 2.736 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.59TrpAla: 0.59 ± 0.024	0.149TrpCys: 0.149 ± 0.012	0.577TrpAsp: 0.577 ± 0.023	0.567TrpGlu: 0.567 ± 0.023	0.423TrpPhe: 0.423 ± 0.021	0.649TrpGly: 0.649 ± 0.025	0.183TrpHis: 0.183 ± 0.014	0.674TrpIle: 0.674 ± 0.027	0.583TrpLys: 0.583 ± 0.025	0.76TrpLeu: 0.76 ± 0.032	0.3TrpMet: 0.3 ± 0.017	0.544TrpAsn: 0.544 ± 0.023	0.22TrpPro: 0.22 ± 0.016	0.331TrpGln: 0.331 ± 0.016	0.362TrpArg: 0.362 ± 0.016	0.465TrpSer: 0.465 ± 0.02	0.46TrpThr: 0.46 ± 0.026	0.537TrpVal: 0.537 ± 0.023	0.133TrpTrp: 0.133 ± 0.012	0.414TrpTyr: 0.414 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.722TyrAla: 2.722 ± 0.05	0.594TyrCys: 0.594 ± 0.02	3.051TyrAsp: 3.051 ± 0.059	3.134TyrGlu: 3.134 ± 0.055	1.944TyrPhe: 1.944 ± 0.044	3.114TyrGly: 3.114 ± 0.052	0.796TyrHis: 0.796 ± 0.026	3.093TyrIle: 3.093 ± 0.061	2.597TyrLys: 2.597 ± 0.045	3.625TyrLeu: 3.625 ± 0.07	1.189TyrMet: 1.189 ± 0.036	2.085TyrAsn: 2.085 ± 0.045	1.358TyrPro: 1.358 ± 0.036	1.222TyrGln: 1.222 ± 0.037	1.939TyrArg: 1.939 ± 0.043	2.679TyrSer: 2.679 ± 0.053	2.307TyrThr: 2.307 ± 0.057	2.873TyrVal: 2.873 ± 0.06	0.374TyrTrp: 0.374 ± 0.018	2.105TyrTyr: 2.105 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3241 proteins (1078809 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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