Candidatus Nitrotoga sp. AM1P

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Gallionellaceae; Candidatus Nitrotoga; unclassified Candidatus Nitrotoga

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455X4R2|A0A455X4R2_9PROT 4Fe-4S ferredoxin OS=Candidatus Nitrotoga sp. AM1P OX=2559597 GN=W01_23910 PE=4 SV=1
MM1 pKa = 7.38TPNPEE6 pKa = 4.11YY7 pKa = 9.42KK8 pKa = 9.97TYY10 pKa = 9.92MCLICGWLYY19 pKa = 11.27DD20 pKa = 4.08EE21 pKa = 5.13ATGSPEE27 pKa = 5.07DD28 pKa = 4.75GILPGTRR35 pKa = 11.84WEE37 pKa = 4.26DD38 pKa = 3.46VPVNWTCPEE47 pKa = 3.86CGARR51 pKa = 11.84KK52 pKa = 9.56EE53 pKa = 4.09DD54 pKa = 3.55FDD56 pKa = 3.85MVEE59 pKa = 4.07FF60 pKa = 4.63

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455WZD2|A0A455WZD2_9PROT NTP_transf_2 domain-containing protein OS=Candidatus Nitrotoga sp. AM1P OX=2559597 GN=W01_03140 PE=4 SV=1
MM1 pKa = 6.98KK2 pKa = 10.26HH3 pKa = 6.63EE4 pKa = 4.25YY5 pKa = 8.93PQPFARR11 pKa = 11.84NYY13 pKa = 9.65EE14 pKa = 4.24SLLKK18 pKa = 10.23RR19 pKa = 11.84LKK21 pKa = 10.65LRR23 pKa = 11.84GMQPLYY29 pKa = 10.91GLKK32 pKa = 10.01FYY34 pKa = 10.28YY35 pKa = 10.56AHH37 pKa = 6.96VLDD40 pKa = 4.45QPWPGAEE47 pKa = 3.88LTKK50 pKa = 10.59APKK53 pKa = 10.09VSRR56 pKa = 11.84LPDD59 pKa = 3.29IVTVAQMQQIINATRR74 pKa = 11.84VLSYY78 pKa = 10.78RR79 pKa = 11.84VFFFTLYY86 pKa = 11.32SMGLRR91 pKa = 11.84LGEE94 pKa = 4.08GLQLRR99 pKa = 11.84VGDD102 pKa = 5.21LDD104 pKa = 3.69AQQMRR109 pKa = 11.84VHH111 pKa = 6.16VRR113 pKa = 11.84DD114 pKa = 3.35AKK116 pKa = 10.58GNRR119 pKa = 11.84DD120 pKa = 3.51RR121 pKa = 11.84LVPLPTHH128 pKa = 4.96TLEE131 pKa = 4.18VLRR134 pKa = 11.84AFWKK138 pKa = 8.75NTATRR143 pKa = 11.84CCCFPAAKK151 pKa = 9.74KK152 pKa = 10.47ASQTQPAPRR161 pKa = 11.84RR162 pKa = 11.84TWIVVGCNKK171 pKa = 9.69RR172 pKa = 11.84CAKK175 pKa = 9.26SRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84SVV181 pKa = 2.72

Molecular weight: 20.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2658

0

2658

833905

42

2812

313.7

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.728 ± 0.05

0.98 ± 0.018

5.125 ± 0.031

5.745 ± 0.046

3.821 ± 0.031

7.181 ± 0.043

2.447 ± 0.026

6.074 ± 0.04

4.76 ± 0.04

10.679 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.022

3.998 ± 0.031

4.409 ± 0.031

4.304 ± 0.042

5.708 ± 0.048

5.893 ± 0.037

5.401 ± 0.051

7.009 ± 0.04

1.324 ± 0.023

2.812 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski