Corynebacterium resistens (strain DSM 45100 / JCM 12819 / GTC 2026 / SICGH 158)
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8DY62|F8DY62_CORRG Putative membrane protein OS=Corynebacterium resistens (strain DSM 45100 / JCM 12819 / GTC 2026 / SICGH 158) OX=662755 GN=CRES_0441 PE=4 SV=1
MM1 pKa = 7.42 NPTRR5 pKa = 11.84 GEE7 pKa = 4.08 TDD9 pKa = 3.36 PSRR12 pKa = 11.84 NPVPSEE18 pKa = 3.53 HH19 pKa = 6.87 HH20 pKa = 6.61 GGDD23 pKa = 3.44 GAATGGHH30 pKa = 7.22 DD31 pKa = 4.32 SPVLTPVEE39 pKa = 3.96 ALVEE43 pKa = 4.26 QFGPPSVSLEE53 pKa = 4.14 DD54 pKa = 3.72 MLQVALDD61 pKa = 4.25 PSTPDD66 pKa = 3.97 PGDD69 pKa = 3.39 EE70 pKa = 5.31 LIPDD74 pKa = 3.99 NDD76 pKa = 4.14 SVHH79 pKa = 7.38 DD80 pKa = 4.08 SASDD84 pKa = 3.23 AGADD88 pKa = 3.23 AGGASGEE95 pKa = 4.42 PDD97 pKa = 4.25 LSDD100 pKa = 5.11 LGDD103 pKa = 3.63 GSYY106 pKa = 11.24 PDD108 pKa = 4.22 ATPGAADD115 pKa = 5.05 DD116 pKa = 4.75 LPSPTGEE123 pKa = 4.04 SEE125 pKa = 4.43 AVGGEE130 pKa = 3.95 HH131 pKa = 7.66 WDD133 pKa = 3.77 TNQPNPQGHH142 pKa = 7.49 DD143 pKa = 3.52 DD144 pKa = 3.89 YY145 pKa = 11.71 GVPGEE150 pKa = 3.88 DD151 pKa = 3.64 HH152 pKa = 7.22 GYY154 pKa = 9.7 GGYY157 pKa = 10.69 DD158 pKa = 3.3 GVHH161 pKa = 7.03 DD162 pKa = 5.03 PNHH165 pKa = 5.83 GTGGHH170 pKa = 4.86 NTHH173 pKa = 7.19 PNAHH177 pKa = 6.01 EE178 pKa = 3.9 TDD180 pKa = 3.49 PFNDD184 pKa = 3.36 EE185 pKa = 4.18 GGIGG189 pKa = 3.32
Molecular weight: 19.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.163
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|F8DXH4|F8DXH4_CORRG Putative oxidoreductase OS=Corynebacterium resistens (strain DSM 45100 / JCM 12819 / GTC 2026 / SICGH 158) OX=662755 GN=CRES_0356 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.22 GRR42 pKa = 11.84 KK43 pKa = 9.07 SLTAA47 pKa = 4.07
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2160
0
2160
755222
14
2667
349.6
37.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.308 ± 0.058
0.732 ± 0.014
5.816 ± 0.043
6.361 ± 0.064
3.215 ± 0.034
8.451 ± 0.043
2.186 ± 0.024
4.867 ± 0.038
3.869 ± 0.043
9.163 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.275 ± 0.023
3.186 ± 0.031
5.099 ± 0.045
3.542 ± 0.03
6.331 ± 0.053
6.069 ± 0.041
6.031 ± 0.047
7.989 ± 0.048
1.372 ± 0.021
2.14 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here