Wenzhou sobemo-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KEG5|A0A1L3KEG5_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 3 OX=1923659 PE=4 SV=1
MM1 pKa = 7.17FKK3 pKa = 10.61AAVMDD8 pKa = 4.72LDD10 pKa = 4.17MNSVPGQCEE19 pKa = 3.52MSRR22 pKa = 11.84FGSTNGEE29 pKa = 4.0ALGWDD34 pKa = 3.42ADD36 pKa = 3.99NYY38 pKa = 10.15SFDD41 pKa = 3.72YY42 pKa = 10.81EE43 pKa = 4.06RR44 pKa = 11.84YY45 pKa = 9.26HH46 pKa = 6.64YY47 pKa = 10.13FKK49 pKa = 10.89QLVKK53 pKa = 10.17TRR55 pKa = 11.84LLDD58 pKa = 3.71VIKK61 pKa = 10.79APVSDD66 pKa = 4.27EE67 pKa = 3.45IKK69 pKa = 10.37IFVKK73 pKa = 10.35QEE75 pKa = 3.56PHH77 pKa = 6.33KK78 pKa = 10.23KK79 pKa = 10.43AKK81 pKa = 9.91VDD83 pKa = 3.32EE84 pKa = 4.48GRR86 pKa = 11.84FRR88 pKa = 11.84LISAVSLVDD97 pKa = 3.62SMVDD101 pKa = 2.64RR102 pKa = 11.84MLFMRR107 pKa = 11.84ITHH110 pKa = 5.92RR111 pKa = 11.84VVPNYY116 pKa = 10.44VNTGVMIGWNPSAGGFRR133 pKa = 11.84YY134 pKa = 10.29LSALFPPGTPLLMADD149 pKa = 3.84RR150 pKa = 11.84SSWDD154 pKa = 2.91WTVQYY159 pKa = 11.76DD160 pKa = 3.72MIVAAKK166 pKa = 10.03EE167 pKa = 4.15VIQRR171 pKa = 11.84LAVGAPAWWSAAVEE185 pKa = 4.13HH186 pKa = 6.73RR187 pKa = 11.84FIALFEE193 pKa = 4.29NPVFRR198 pKa = 11.84FSDD201 pKa = 3.93GEE203 pKa = 4.17RR204 pKa = 11.84IMQQVPGIMKK214 pKa = 9.82SGCYY218 pKa = 8.46LTILLNSLIQLLLHH232 pKa = 6.42YY233 pKa = 9.5EE234 pKa = 4.1LSDD237 pKa = 3.51NPNIVVLGDD246 pKa = 3.5DD247 pKa = 4.04TLQEE251 pKa = 4.67DD252 pKa = 4.14EE253 pKa = 4.75DD254 pKa = 4.46DD255 pKa = 4.55EE256 pKa = 4.61YY257 pKa = 11.82LEE259 pKa = 4.15KK260 pKa = 11.01LRR262 pKa = 11.84DD263 pKa = 3.73FGITLKK269 pKa = 9.18VHH271 pKa = 5.72RR272 pKa = 11.84GEE274 pKa = 4.52RR275 pKa = 11.84IEE277 pKa = 4.07FAGFYY282 pKa = 10.17VDD284 pKa = 3.51EE285 pKa = 4.73KK286 pKa = 10.92GYY288 pKa = 10.77YY289 pKa = 8.16PAYY292 pKa = 9.08EE293 pKa = 4.5DD294 pKa = 3.11KK295 pKa = 11.09HH296 pKa = 7.02VFQLEE301 pKa = 4.37HH302 pKa = 6.17MCIDD306 pKa = 3.99DD307 pKa = 4.1PEE309 pKa = 5.23KK310 pKa = 11.06LGATLEE316 pKa = 4.25NYY318 pKa = 9.91QILYY322 pKa = 10.3ACVPAKK328 pKa = 10.68LKK330 pKa = 10.44VLRR333 pKa = 11.84SMVEE337 pKa = 3.73EE338 pKa = 3.9YY339 pKa = 11.16GLYY342 pKa = 10.49EE343 pKa = 4.22NYY345 pKa = 10.06ISDD348 pKa = 3.37GRR350 pKa = 11.84LRR352 pKa = 11.84GLKK355 pKa = 10.19HH356 pKa = 5.61GG357 pKa = 4.39

Molecular weight:
41.0 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KEG5|A0A1L3KEG5_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 3 OX=1923659 PE=4 SV=1
MM1 pKa = 7.66VSRR4 pKa = 11.84TDD6 pKa = 3.31TLEE9 pKa = 4.42AGLGTEE15 pKa = 4.78TIWSRR20 pKa = 11.84VFTLLTNTNVSKK32 pKa = 10.75SASFVARR39 pKa = 11.84EE40 pKa = 4.1KK41 pKa = 9.88VSKK44 pKa = 10.59YY45 pKa = 10.73YY46 pKa = 9.74EE47 pKa = 4.42TILCLGHH54 pKa = 7.14AISPGRR60 pKa = 11.84LLIHH64 pKa = 6.5VNGANWVFQQLNWCLPCLQRR84 pKa = 11.84SKK86 pKa = 10.8PQLTMVCNLLKK97 pKa = 10.57AVSRR101 pKa = 11.84SYY103 pKa = 11.12QYY105 pKa = 11.61LVTCLTQDD113 pKa = 3.06QHH115 pKa = 8.18GLGFRR120 pKa = 11.84VLHH123 pKa = 6.49IMLTTTYY130 pKa = 8.78MPCMSDD136 pKa = 3.17QVVLILEE143 pKa = 4.31LAVRR147 pKa = 11.84IYY149 pKa = 10.61MRR151 pKa = 11.84FCAWKK156 pKa = 9.24IRR158 pKa = 11.84RR159 pKa = 11.84TSSYY163 pKa = 8.58KK164 pKa = 9.67TSRR167 pKa = 11.84PLPANDD173 pKa = 3.15GSTRR177 pKa = 11.84VLAQLTLLNDD187 pKa = 3.55WWNTMAVCMFLIEE200 pKa = 5.32KK201 pKa = 9.5ILLMLSSILNGMTKK215 pKa = 10.6

Molecular weight:
24.51 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1087

215

515

362.3

40.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.82 ± 1.221

1.472 ± 0.703

4.968 ± 1.148

5.244 ± 0.95

3.864 ± 0.453

5.98 ± 0.583

1.84 ± 0.312

4.14 ± 0.7

4.324 ± 0.443

9.936 ± 1.633

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.312 ± 0.582

3.588 ± 0.331

5.152 ± 0.987

3.68 ± 0.274

5.98 ± 0.167

7.82 ± 1.128

5.796 ± 1.499

8.464 ± 0.406

1.656 ± 0.373

4.968 ± 0.533

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski