Wenzhou sobemo-like virus 3
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEG5|A0A1L3KEG5_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 3 OX=1923659 PE=4 SV=1
MM1 pKa = 7.17 FKK3 pKa = 10.61 AAVMDD8 pKa = 4.72 LDD10 pKa = 4.17 MNSVPGQCEE19 pKa = 3.52 MSRR22 pKa = 11.84 FGSTNGEE29 pKa = 4.0 ALGWDD34 pKa = 3.42 ADD36 pKa = 3.99 NYY38 pKa = 10.15 SFDD41 pKa = 3.72 YY42 pKa = 10.81 EE43 pKa = 4.06 RR44 pKa = 11.84 YY45 pKa = 9.26 HH46 pKa = 6.64 YY47 pKa = 10.13 FKK49 pKa = 10.89 QLVKK53 pKa = 10.17 TRR55 pKa = 11.84 LLDD58 pKa = 3.71 VIKK61 pKa = 10.79 APVSDD66 pKa = 4.27 EE67 pKa = 3.45 IKK69 pKa = 10.37 IFVKK73 pKa = 10.35 QEE75 pKa = 3.56 PHH77 pKa = 6.33 KK78 pKa = 10.23 KK79 pKa = 10.43 AKK81 pKa = 9.91 VDD83 pKa = 3.32 EE84 pKa = 4.48 GRR86 pKa = 11.84 FRR88 pKa = 11.84 LISAVSLVDD97 pKa = 3.62 SMVDD101 pKa = 2.64 RR102 pKa = 11.84 MLFMRR107 pKa = 11.84 ITHH110 pKa = 5.92 RR111 pKa = 11.84 VVPNYY116 pKa = 10.44 VNTGVMIGWNPSAGGFRR133 pKa = 11.84 YY134 pKa = 10.29 LSALFPPGTPLLMADD149 pKa = 3.84 RR150 pKa = 11.84 SSWDD154 pKa = 2.91 WTVQYY159 pKa = 11.76 DD160 pKa = 3.72 MIVAAKK166 pKa = 10.03 EE167 pKa = 4.15 VIQRR171 pKa = 11.84 LAVGAPAWWSAAVEE185 pKa = 4.13 HH186 pKa = 6.73 RR187 pKa = 11.84 FIALFEE193 pKa = 4.29 NPVFRR198 pKa = 11.84 FSDD201 pKa = 3.93 GEE203 pKa = 4.17 RR204 pKa = 11.84 IMQQVPGIMKK214 pKa = 9.82 SGCYY218 pKa = 8.46 LTILLNSLIQLLLHH232 pKa = 6.42 YY233 pKa = 9.5 EE234 pKa = 4.1 LSDD237 pKa = 3.51 NPNIVVLGDD246 pKa = 3.5 DD247 pKa = 4.04 TLQEE251 pKa = 4.67 DD252 pKa = 4.14 EE253 pKa = 4.75 DD254 pKa = 4.46 DD255 pKa = 4.55 EE256 pKa = 4.61 YY257 pKa = 11.82 LEE259 pKa = 4.15 KK260 pKa = 11.01 LRR262 pKa = 11.84 DD263 pKa = 3.73 FGITLKK269 pKa = 9.18 VHH271 pKa = 5.72 RR272 pKa = 11.84 GEE274 pKa = 4.52 RR275 pKa = 11.84 IEE277 pKa = 4.07 FAGFYY282 pKa = 10.17 VDD284 pKa = 3.51 EE285 pKa = 4.73 KK286 pKa = 10.92 GYY288 pKa = 10.77 YY289 pKa = 8.16 PAYY292 pKa = 9.08 EE293 pKa = 4.5 DD294 pKa = 3.11 KK295 pKa = 11.09 HH296 pKa = 7.02 VFQLEE301 pKa = 4.37 HH302 pKa = 6.17 MCIDD306 pKa = 3.99 DD307 pKa = 4.1 PEE309 pKa = 5.23 KK310 pKa = 11.06 LGATLEE316 pKa = 4.25 NYY318 pKa = 9.91 QILYY322 pKa = 10.3 ACVPAKK328 pKa = 10.68 LKK330 pKa = 10.44 VLRR333 pKa = 11.84 SMVEE337 pKa = 3.73 EE338 pKa = 3.9 YY339 pKa = 11.16 GLYY342 pKa = 10.49 EE343 pKa = 4.22 NYY345 pKa = 10.06 ISDD348 pKa = 3.37 GRR350 pKa = 11.84 LRR352 pKa = 11.84 GLKK355 pKa = 10.19 HH356 pKa = 5.61 GG357 pKa = 4.39
Molecular weight: 41.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.113
IPC2_protein 5.181
IPC_protein 5.143
Toseland 5.169
ProMoST 5.321
Dawson 5.181
Bjellqvist 5.296
Wikipedia 5.08
Rodwell 5.105
Grimsley 5.118
Solomon 5.181
Lehninger 5.143
Nozaki 5.321
DTASelect 5.499
Thurlkill 5.181
EMBOSS 5.156
Sillero 5.397
Patrickios 3.884
IPC_peptide 5.194
IPC2_peptide 5.397
IPC2.peptide.svr19 5.352
Protein with the highest isoelectric point:
>tr|A0A1L3KEG5|A0A1L3KEG5_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 3 OX=1923659 PE=4 SV=1
MM1 pKa = 7.66 VSRR4 pKa = 11.84 TDD6 pKa = 3.31 TLEE9 pKa = 4.42 AGLGTEE15 pKa = 4.78 TIWSRR20 pKa = 11.84 VFTLLTNTNVSKK32 pKa = 10.75 SASFVARR39 pKa = 11.84 EE40 pKa = 4.1 KK41 pKa = 9.88 VSKK44 pKa = 10.59 YY45 pKa = 10.73 YY46 pKa = 9.74 EE47 pKa = 4.42 TILCLGHH54 pKa = 7.14 AISPGRR60 pKa = 11.84 LLIHH64 pKa = 6.5 VNGANWVFQQLNWCLPCLQRR84 pKa = 11.84 SKK86 pKa = 10.8 PQLTMVCNLLKK97 pKa = 10.57 AVSRR101 pKa = 11.84 SYY103 pKa = 11.12 QYY105 pKa = 11.61 LVTCLTQDD113 pKa = 3.06 QHH115 pKa = 8.18 GLGFRR120 pKa = 11.84 VLHH123 pKa = 6.49 IMLTTTYY130 pKa = 8.78 MPCMSDD136 pKa = 3.17 QVVLILEE143 pKa = 4.31 LAVRR147 pKa = 11.84 IYY149 pKa = 10.61 MRR151 pKa = 11.84 FCAWKK156 pKa = 9.24 IRR158 pKa = 11.84 RR159 pKa = 11.84 TSSYY163 pKa = 8.58 KK164 pKa = 9.67 TSRR167 pKa = 11.84 PLPANDD173 pKa = 3.15 GSTRR177 pKa = 11.84 VLAQLTLLNDD187 pKa = 3.55 WWNTMAVCMFLIEE200 pKa = 5.32 KK201 pKa = 9.5 ILLMLSSILNGMTKK215 pKa = 10.6
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.91
IPC2_protein 8.799
IPC_protein 8.77
Toseland 9.165
ProMoST 9.282
Dawson 9.545
Bjellqvist 9.502
Wikipedia 9.721
Rodwell 9.677
Grimsley 9.56
Solomon 9.633
Lehninger 9.589
Nozaki 9.663
DTASelect 9.355
Thurlkill 9.443
EMBOSS 9.677
Sillero 9.648
Patrickios 4.507
IPC_peptide 9.619
IPC2_peptide 8.712
IPC2.peptide.svr19 7.864
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1087
215
515
362.3
40.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.82 ± 1.221
1.472 ± 0.703
4.968 ± 1.148
5.244 ± 0.95
3.864 ± 0.453
5.98 ± 0.583
1.84 ± 0.312
4.14 ± 0.7
4.324 ± 0.443
9.936 ± 1.633
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.312 ± 0.582
3.588 ± 0.331
5.152 ± 0.987
3.68 ± 0.274
5.98 ± 0.167
7.82 ± 1.128
5.796 ± 1.499
8.464 ± 0.406
1.656 ± 0.373
4.968 ± 0.533
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here