Bacillus phage phiCM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Camtrevirus; Bacillus virus phiCM3

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8CYG4|W8CYG4_9CAUD Uncharacterized protein OS=Bacillus phage phiCM3 OX=1357713 GN=phiCM3_gp36 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.53LNGLIGGIEE11 pKa = 3.88MTKK14 pKa = 10.67EE15 pKa = 3.82EE16 pKa = 4.28IVEE19 pKa = 4.21LFLNTIDD26 pKa = 3.52EE27 pKa = 4.68TKK29 pKa = 10.47PEE31 pKa = 4.76LIEE34 pKa = 4.22EE35 pKa = 4.42YY36 pKa = 9.96IEE38 pKa = 3.89EE39 pKa = 4.2SFRR42 pKa = 11.84II43 pKa = 4.0

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8CZ42|W8CZ42_9CAUD Uncharacterized protein OS=Bacillus phage phiCM3 OX=1357713 GN=phiCM3_gp02 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.35VLRR5 pKa = 11.84DD6 pKa = 3.54QLRR9 pKa = 11.84EE10 pKa = 3.91WKK12 pKa = 10.63SNQTKK17 pKa = 10.61KK18 pKa = 9.12KK19 pKa = 7.5TKK21 pKa = 9.81KK22 pKa = 9.55KK23 pKa = 10.03RR24 pKa = 11.84KK25 pKa = 8.24EE26 pKa = 3.98KK27 pKa = 10.76LSTRR31 pKa = 11.84QIEE34 pKa = 4.42DD35 pKa = 2.97LMEE38 pKa = 4.04GHH40 pKa = 6.7RR41 pKa = 11.84PCYY44 pKa = 8.2EE45 pKa = 3.49RR46 pKa = 11.84RR47 pKa = 11.84YY48 pKa = 9.68GAIRR52 pKa = 11.84EE53 pKa = 4.23KK54 pKa = 11.11

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10625

34

1308

189.7

21.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.278 ± 0.621

0.951 ± 0.2

5.318 ± 0.279

8.433 ± 0.354

4.094 ± 0.257

5.769 ± 0.377

1.675 ± 0.197

6.842 ± 0.258

9.468 ± 0.272

7.727 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.247 ± 0.2

5.948 ± 0.261

2.795 ± 0.303

4.424 ± 0.282

4.668 ± 0.266

5.704 ± 0.234

5.525 ± 0.413

6.174 ± 0.238

1.261 ± 0.157

3.699 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski