Amino acid dipepetide frequency for Bacillus phage phiCM3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.177AlaAla: 5.177 ± 0.924
1.035AlaCys: 1.035 ± 0.375
3.294AlaAsp: 3.294 ± 0.677
4.142AlaGlu: 4.142 ± 0.877
1.977AlaPhe: 1.977 ± 0.375
4.047AlaGly: 4.047 ± 0.779
0.659AlaHis: 0.659 ± 0.262
4.518AlaIle: 4.518 ± 0.579
6.777AlaLys: 6.777 ± 0.802
5.177AlaLeu: 5.177 ± 0.767
2.259AlaMet: 2.259 ± 0.439
3.012AlaAsn: 3.012 ± 0.711
1.506AlaPro: 1.506 ± 0.293
2.071AlaGln: 2.071 ± 0.52
2.824AlaArg: 2.824 ± 0.563
2.73AlaSer: 2.73 ± 0.81
4.047AlaThr: 4.047 ± 1.14
4.518AlaVal: 4.518 ± 1.157
0.659AlaTrp: 0.659 ± 0.246
2.259AlaTyr: 2.259 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
0.471CysAla: 0.471 ± 0.2
0.188CysCys: 0.188 ± 0.14
1.035CysAsp: 1.035 ± 0.33
0.659CysGlu: 0.659 ± 0.215
0.377CysPhe: 0.377 ± 0.236
0.659CysGly: 0.659 ± 0.285
0.377CysHis: 0.377 ± 0.261
0.282CysIle: 0.282 ± 0.153
0.753CysLys: 0.753 ± 0.273
0.471CysLeu: 0.471 ± 0.274
0.659CysMet: 0.659 ± 0.271
0.282CysAsn: 0.282 ± 0.155
0.471CysPro: 0.471 ± 0.251
0.471CysGln: 0.471 ± 0.256
0.377CysArg: 0.377 ± 0.197
0.753CysSer: 0.753 ± 0.275
0.471CysThr: 0.471 ± 0.163
0.282CysVal: 0.282 ± 0.175
0.0CysTrp: 0.0 ± 0.0
0.471CysTyr: 0.471 ± 0.278
0.0CysXaa: 0.0 ± 0.0
Asp
3.012AspAla: 3.012 ± 0.733
0.188AspCys: 0.188 ± 0.141
2.824AspAsp: 2.824 ± 0.542
4.8AspGlu: 4.8 ± 0.627
2.259AspPhe: 2.259 ± 0.371
3.765AspGly: 3.765 ± 0.571
0.941AspHis: 0.941 ± 0.299
3.671AspIle: 3.671 ± 0.509
5.553AspLys: 5.553 ± 0.505
4.518AspLeu: 4.518 ± 0.549
2.541AspMet: 2.541 ± 0.68
2.353AspAsn: 2.353 ± 0.467
1.6AspPro: 1.6 ± 0.384
1.318AspGln: 1.318 ± 0.383
1.788AspArg: 1.788 ± 0.448
2.541AspSer: 2.541 ± 0.449
2.353AspThr: 2.353 ± 0.489
3.483AspVal: 3.483 ± 0.677
0.941AspTrp: 0.941 ± 0.254
2.73AspTyr: 2.73 ± 0.519
0.0AspXaa: 0.0 ± 0.0
Glu
5.836GluAla: 5.836 ± 0.668
0.847GluCys: 0.847 ± 0.354
3.953GluAsp: 3.953 ± 0.832
7.624GluGlu: 7.624 ± 0.915
3.2GluPhe: 3.2 ± 0.615
4.047GluGly: 4.047 ± 0.61
1.506GluHis: 1.506 ± 0.444
7.53GluIle: 7.53 ± 0.566
8.377GluLys: 8.377 ± 0.693
7.436GluLeu: 7.436 ± 0.754
2.73GluMet: 2.73 ± 0.451
4.047GluAsn: 4.047 ± 0.765
1.788GluPro: 1.788 ± 0.493
4.33GluGln: 4.33 ± 0.615
4.989GluArg: 4.989 ± 0.892
3.765GluSer: 3.765 ± 0.717
4.047GluThr: 4.047 ± 0.601
4.518GluVal: 4.518 ± 0.696
0.847GluTrp: 0.847 ± 0.291
2.918GluTyr: 2.918 ± 0.529
0.0GluXaa: 0.0 ± 0.0
Phe
2.165PheAla: 2.165 ± 0.464
0.188PheCys: 0.188 ± 0.137
1.977PheAsp: 1.977 ± 0.463
3.2PheGlu: 3.2 ± 0.543
1.6PhePhe: 1.6 ± 0.385
3.106PheGly: 3.106 ± 0.494
0.941PheHis: 0.941 ± 0.294
2.918PheIle: 2.918 ± 0.447
4.047PheLys: 4.047 ± 0.64
3.859PheLeu: 3.859 ± 0.612
1.318PheMet: 1.318 ± 0.34
2.447PheAsn: 2.447 ± 0.399
0.941PhePro: 0.941 ± 0.341
1.412PheGln: 1.412 ± 0.304
1.694PheArg: 1.694 ± 0.348
2.636PheSer: 2.636 ± 0.457
2.447PheThr: 2.447 ± 0.613
1.977PheVal: 1.977 ± 0.398
0.659PheTrp: 0.659 ± 0.239
1.412PheTyr: 1.412 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
2.73GlyAla: 2.73 ± 0.631
0.282GlyCys: 0.282 ± 0.159
2.447GlyAsp: 2.447 ± 0.325
5.083GlyGlu: 5.083 ± 0.653
3.012GlyPhe: 3.012 ± 0.6
3.483GlyGly: 3.483 ± 0.606
0.565GlyHis: 0.565 ± 0.207
4.518GlyIle: 4.518 ± 0.815
5.365GlyLys: 5.365 ± 0.713
3.765GlyLeu: 3.765 ± 0.647
2.353GlyMet: 2.353 ± 0.658
3.012GlyAsn: 3.012 ± 0.551
0.753GlyPro: 0.753 ± 0.256
2.165GlyGln: 2.165 ± 0.494
3.389GlyArg: 3.389 ± 0.479
3.859GlySer: 3.859 ± 0.422
3.012GlyThr: 3.012 ± 0.468
4.236GlyVal: 4.236 ± 0.626
1.224GlyTrp: 1.224 ± 0.349
2.447GlyTyr: 2.447 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
0.659HisAla: 0.659 ± 0.24
0.282HisCys: 0.282 ± 0.157
0.282HisAsp: 0.282 ± 0.139
1.6HisGlu: 1.6 ± 0.296
0.471HisPhe: 0.471 ± 0.218
0.847HisGly: 0.847 ± 0.329
0.377HisHis: 0.377 ± 0.239
1.13HisIle: 1.13 ± 0.29
1.694HisLys: 1.694 ± 0.432
1.694HisLeu: 1.694 ± 0.406
0.565HisMet: 0.565 ± 0.184
0.847HisAsn: 0.847 ± 0.315
0.188HisPro: 0.188 ± 0.167
0.659HisGln: 0.659 ± 0.277
0.847HisArg: 0.847 ± 0.295
1.977HisSer: 1.977 ± 0.507
1.224HisThr: 1.224 ± 0.363
0.847HisVal: 0.847 ± 0.232
0.094HisTrp: 0.094 ± 0.097
0.471HisTyr: 0.471 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.742IleAla: 5.742 ± 0.691
0.471IleCys: 0.471 ± 0.174
3.577IleAsp: 3.577 ± 0.572
7.154IleGlu: 7.154 ± 0.774
2.165IlePhe: 2.165 ± 0.468
3.294IleGly: 3.294 ± 0.568
1.13IleHis: 1.13 ± 0.309
4.424IleIle: 4.424 ± 0.633
6.401IleLys: 6.401 ± 0.775
3.859IleLeu: 3.859 ± 0.578
2.447IleMet: 2.447 ± 0.395
4.706IleAsn: 4.706 ± 0.64
2.071IlePro: 2.071 ± 0.355
3.012IleGln: 3.012 ± 0.448
3.294IleArg: 3.294 ± 0.439
3.671IleSer: 3.671 ± 0.492
4.236IleThr: 4.236 ± 0.618
3.765IleVal: 3.765 ± 0.614
0.659IleTrp: 0.659 ± 0.267
2.353IleTyr: 2.353 ± 0.522
0.0IleXaa: 0.0 ± 0.0
Lys
6.589LysAla: 6.589 ± 0.816
0.941LysCys: 0.941 ± 0.312
4.989LysAsp: 4.989 ± 0.831
8.283LysGlu: 8.283 ± 0.752
3.671LysPhe: 3.671 ± 0.581
3.953LysGly: 3.953 ± 0.597
2.071LysHis: 2.071 ± 0.456
6.212LysIle: 6.212 ± 0.457
9.319LysLys: 9.319 ± 1.046
6.965LysLeu: 6.965 ± 0.774
2.73LysMet: 2.73 ± 0.687
5.177LysAsn: 5.177 ± 0.651
2.636LysPro: 2.636 ± 0.573
5.271LysGln: 5.271 ± 0.672
4.8LysArg: 4.8 ± 0.63
4.424LysSer: 4.424 ± 0.61
5.271LysThr: 5.271 ± 0.614
7.059LysVal: 7.059 ± 0.722
1.13LysTrp: 1.13 ± 0.344
3.2LysTyr: 3.2 ± 0.471
0.0LysXaa: 0.0 ± 0.0
Leu
4.989LeuAla: 4.989 ± 0.719
1.13LeuCys: 1.13 ± 0.428
4.8LeuAsp: 4.8 ± 0.535
6.024LeuGlu: 6.024 ± 1.045
2.636LeuPhe: 2.636 ± 0.418
3.577LeuGly: 3.577 ± 0.695
1.412LeuHis: 1.412 ± 0.353
4.33LeuIle: 4.33 ± 0.67
7.812LeuLys: 7.812 ± 0.905
5.271LeuLeu: 5.271 ± 0.932
1.6LeuMet: 1.6 ± 0.393
5.553LeuAsn: 5.553 ± 0.804
2.447LeuPro: 2.447 ± 0.55
3.765LeuGln: 3.765 ± 0.506
4.142LeuArg: 4.142 ± 0.608
6.871LeuSer: 6.871 ± 0.76
3.294LeuThr: 3.294 ± 0.587
4.33LeuVal: 4.33 ± 0.671
0.847LeuTrp: 0.847 ± 0.256
2.447LeuTyr: 2.447 ± 0.585
0.0LeuXaa: 0.0 ± 0.0
Met
1.694MetAla: 1.694 ± 0.369
0.282MetCys: 0.282 ± 0.176
1.506MetAsp: 1.506 ± 0.32
3.2MetGlu: 3.2 ± 0.466
0.941MetPhe: 0.941 ± 0.484
2.071MetGly: 2.071 ± 0.504
0.188MetHis: 0.188 ± 0.142
1.6MetIle: 1.6 ± 0.335
4.8MetLys: 4.8 ± 0.618
2.73MetLeu: 2.73 ± 0.56
0.847MetMet: 0.847 ± 0.219
2.259MetAsn: 2.259 ± 0.475
0.941MetPro: 0.941 ± 0.239
1.694MetGln: 1.694 ± 0.358
1.506MetArg: 1.506 ± 0.29
1.318MetSer: 1.318 ± 0.295
2.071MetThr: 2.071 ± 0.411
1.224MetVal: 1.224 ± 0.301
0.377MetTrp: 0.377 ± 0.171
1.224MetTyr: 1.224 ± 0.434
0.0MetXaa: 0.0 ± 0.0
Asn
4.047AsnAla: 4.047 ± 0.668
0.377AsnCys: 0.377 ± 0.196
2.259AsnAsp: 2.259 ± 0.424
5.553AsnGlu: 5.553 ± 0.644
1.883AsnPhe: 1.883 ± 0.408
4.047AsnGly: 4.047 ± 0.637
1.035AsnHis: 1.035 ± 0.278
4.33AsnIle: 4.33 ± 0.712
4.706AsnLys: 4.706 ± 0.907
3.859AsnLeu: 3.859 ± 0.742
2.071AsnMet: 2.071 ± 0.36
3.389AsnAsn: 3.389 ± 0.488
2.541AsnPro: 2.541 ± 0.504
3.012AsnGln: 3.012 ± 0.403
3.012AsnArg: 3.012 ± 0.642
3.765AsnSer: 3.765 ± 0.513
3.859AsnThr: 3.859 ± 0.593
3.765AsnVal: 3.765 ± 0.443
0.565AsnTrp: 0.565 ± 0.201
1.412AsnTyr: 1.412 ± 0.395
0.0AsnXaa: 0.0 ± 0.0
Pro
1.224ProAla: 1.224 ± 0.264
0.377ProCys: 0.377 ± 0.215
1.506ProAsp: 1.506 ± 0.401
2.353ProGlu: 2.353 ± 0.555
1.13ProPhe: 1.13 ± 0.401
1.318ProGly: 1.318 ± 0.323
0.847ProHis: 0.847 ± 0.195
2.353ProIle: 2.353 ± 0.604
1.506ProLys: 1.506 ± 0.393
2.165ProLeu: 2.165 ± 0.516
0.188ProMet: 0.188 ± 0.101
1.977ProAsn: 1.977 ± 0.435
1.318ProPro: 1.318 ± 0.447
0.847ProGln: 0.847 ± 0.268
0.753ProArg: 0.753 ± 0.271
2.259ProSer: 2.259 ± 0.449
2.165ProThr: 2.165 ± 0.439
1.506ProVal: 1.506 ± 0.56
0.282ProTrp: 0.282 ± 0.154
1.883ProTyr: 1.883 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
3.012GlnAla: 3.012 ± 0.891
0.282GlnCys: 0.282 ± 0.158
2.071GlnAsp: 2.071 ± 0.484
2.636GlnGlu: 2.636 ± 0.542
1.694GlnPhe: 1.694 ± 0.501
2.353GlnGly: 2.353 ± 0.457
0.471GlnHis: 0.471 ± 0.22
3.012GlnIle: 3.012 ± 0.447
5.083GlnLys: 5.083 ± 0.879
2.824GlnLeu: 2.824 ± 0.579
1.883GlnMet: 1.883 ± 0.348
3.2GlnAsn: 3.2 ± 0.422
1.694GlnPro: 1.694 ± 0.293
2.73GlnGln: 2.73 ± 0.488
2.071GlnArg: 2.071 ± 0.324
2.353GlnSer: 2.353 ± 0.483
2.071GlnThr: 2.071 ± 0.358
2.165GlnVal: 2.165 ± 0.472
0.659GlnTrp: 0.659 ± 0.216
1.977GlnTyr: 1.977 ± 0.343
0.0GlnXaa: 0.0 ± 0.0
Arg
2.165ArgAla: 2.165 ± 0.51
0.377ArgCys: 0.377 ± 0.145
3.294ArgAsp: 3.294 ± 0.566
4.236ArgGlu: 4.236 ± 0.614
2.73ArgPhe: 2.73 ± 0.438
2.918ArgGly: 2.918 ± 0.475
0.377ArgHis: 0.377 ± 0.194
3.389ArgIle: 3.389 ± 0.649
4.047ArgLys: 4.047 ± 0.539
4.33ArgLeu: 4.33 ± 0.683
1.412ArgMet: 1.412 ± 0.293
2.165ArgAsn: 2.165 ± 0.499
1.318ArgPro: 1.318 ± 0.391
2.447ArgGln: 2.447 ± 0.589
2.353ArgArg: 2.353 ± 0.491
2.071ArgSer: 2.071 ± 0.43
1.788ArgThr: 1.788 ± 0.47
2.447ArgVal: 2.447 ± 0.461
1.035ArgTrp: 1.035 ± 0.272
1.788ArgTyr: 1.788 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
3.294SerAla: 3.294 ± 0.769
0.282SerCys: 0.282 ± 0.163
4.047SerAsp: 4.047 ± 0.543
4.424SerGlu: 4.424 ± 0.535
3.012SerPhe: 3.012 ± 0.646
3.859SerGly: 3.859 ± 0.509
1.224SerHis: 1.224 ± 0.376
4.33SerIle: 4.33 ± 0.555
4.33SerLys: 4.33 ± 0.65
4.142SerLeu: 4.142 ± 0.614
1.788SerMet: 1.788 ± 0.416
3.953SerAsn: 3.953 ± 0.561
1.6SerPro: 1.6 ± 0.401
1.694SerGln: 1.694 ± 0.331
2.071SerArg: 2.071 ± 0.439
3.294SerSer: 3.294 ± 0.588
3.106SerThr: 3.106 ± 0.606
4.047SerVal: 4.047 ± 0.614
0.565SerTrp: 0.565 ± 0.297
1.977SerTyr: 1.977 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
2.918ThrAla: 2.918 ± 0.651
0.565ThrCys: 0.565 ± 0.241
2.73ThrAsp: 2.73 ± 0.55
4.895ThrGlu: 4.895 ± 0.788
2.824ThrPhe: 2.824 ± 0.48
3.483ThrGly: 3.483 ± 0.626
0.941ThrHis: 0.941 ± 0.219
3.389ThrIle: 3.389 ± 0.661
5.93ThrLys: 5.93 ± 0.628
5.177ThrLeu: 5.177 ± 0.688
1.13ThrMet: 1.13 ± 0.271
2.918ThrAsn: 2.918 ± 0.639
1.6ThrPro: 1.6 ± 0.43
1.506ThrGln: 1.506 ± 0.316
1.694ThrArg: 1.694 ± 0.33
3.012ThrSer: 3.012 ± 0.557
4.142ThrThr: 4.142 ± 0.877
4.047ThrVal: 4.047 ± 0.351
0.565ThrTrp: 0.565 ± 0.204
1.788ThrTyr: 1.788 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
4.236ValAla: 4.236 ± 0.679
0.471ValCys: 0.471 ± 0.239
4.33ValAsp: 4.33 ± 0.55
4.989ValGlu: 4.989 ± 0.892
2.353ValPhe: 2.353 ± 0.527
4.236ValGly: 4.236 ± 0.633
0.659ValHis: 0.659 ± 0.342
3.389ValIle: 3.389 ± 0.464
3.953ValLys: 3.953 ± 0.667
4.8ValLeu: 4.8 ± 0.732
2.259ValMet: 2.259 ± 0.373
4.424ValAsn: 4.424 ± 0.486
1.6ValPro: 1.6 ± 0.375
3.389ValGln: 3.389 ± 0.424
2.165ValArg: 2.165 ± 0.501
3.953ValSer: 3.953 ± 0.637
3.294ValThr: 3.294 ± 0.45
3.577ValVal: 3.577 ± 0.724
0.941ValTrp: 0.941 ± 0.346
2.73ValTyr: 2.73 ± 0.545
0.0ValXaa: 0.0 ± 0.0
Trp
0.753TrpAla: 0.753 ± 0.309
0.188TrpCys: 0.188 ± 0.145
0.847TrpAsp: 0.847 ± 0.233
0.941TrpGlu: 0.941 ± 0.259
0.659TrpPhe: 0.659 ± 0.259
0.565TrpGly: 0.565 ± 0.273
0.188TrpHis: 0.188 ± 0.11
0.753TrpIle: 0.753 ± 0.251
0.847TrpLys: 0.847 ± 0.255
0.847TrpLeu: 0.847 ± 0.247
0.377TrpMet: 0.377 ± 0.195
0.941TrpAsn: 0.941 ± 0.226
0.0TrpPro: 0.0 ± 0.0
0.753TrpGln: 0.753 ± 0.288
0.847TrpArg: 0.847 ± 0.223
0.753TrpSer: 0.753 ± 0.236
0.659TrpThr: 0.659 ± 0.24
1.035TrpVal: 1.035 ± 0.32
0.094TrpTrp: 0.094 ± 0.081
0.565TrpTyr: 0.565 ± 0.219
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.071TyrAla: 2.071 ± 0.43
0.753TyrCys: 0.753 ± 0.249
1.412TyrAsp: 1.412 ± 0.411
2.541TyrGlu: 2.541 ± 0.479
2.353TyrPhe: 2.353 ± 0.466
2.071TyrGly: 2.071 ± 0.433
0.847TyrHis: 0.847 ± 0.297
2.353TyrIle: 2.353 ± 0.489
3.389TyrLys: 3.389 ± 0.685
3.389TyrLeu: 3.389 ± 0.823
1.224TyrMet: 1.224 ± 0.342
2.824TyrAsn: 2.824 ± 0.575
0.941TyrPro: 0.941 ± 0.342
1.694TyrGln: 1.694 ± 0.321
2.071TyrArg: 2.071 ± 0.399
1.035TyrSer: 1.035 ± 0.309
1.694TyrThr: 1.694 ± 0.387
2.918TyrVal: 2.918 ± 0.553
0.471TyrTrp: 0.471 ± 0.185
0.941TyrTyr: 0.941 ± 0.414
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (10625 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski