Spider associated circular virus 3
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPE2|A0A346BPE2_9VIRU Putative capsid protein OS=Spider associated circular virus 3 OX=2293304 PE=4 SV=1
MM1 pKa = 7.36 SRR3 pKa = 11.84 TRR5 pKa = 11.84 NWCFTIFTEE14 pKa = 5.48 DD15 pKa = 3.37 GDD17 pKa = 4.67 GEE19 pKa = 4.32 ASAHH23 pKa = 5.89 YY24 pKa = 10.23 DD25 pKa = 3.33 PNTIDD30 pKa = 3.71 WLSATPQVRR39 pKa = 11.84 YY40 pKa = 9.92 VVAGLEE46 pKa = 4.15 VAPTSGRR53 pKa = 11.84 LHH55 pKa = 5.05 WQGYY59 pKa = 9.75 IEE61 pKa = 4.5 LQHH64 pKa = 6.69 PATLVSCKK72 pKa = 10.39 NSLGTDD78 pKa = 3.35 KK79 pKa = 11.32 AHH81 pKa = 7.11 LEE83 pKa = 3.81 IRR85 pKa = 11.84 RR86 pKa = 11.84 GTRR89 pKa = 11.84 NQAIAYY95 pKa = 7.67 CLKK98 pKa = 10.2 QDD100 pKa = 3.7 SAAQGEE106 pKa = 4.6 PEE108 pKa = 4.22 GNEE111 pKa = 3.67 PKK113 pKa = 10.28 IIFTWGDD120 pKa = 3.76 DD121 pKa = 3.93 NPQGTDD127 pKa = 3.47 DD128 pKa = 4.64 IYY130 pKa = 11.69 DD131 pKa = 3.67 EE132 pKa = 4.77 VLCQTDD138 pKa = 3.74 VNTAIQFIKK147 pKa = 10.24 RR148 pKa = 11.84 VRR150 pKa = 11.84 PRR152 pKa = 11.84 DD153 pKa = 3.43 YY154 pKa = 10.97 CIFGQQLHH162 pKa = 6.38 TNLEE166 pKa = 4.02 RR167 pKa = 11.84 HH168 pKa = 4.47 FTKK171 pKa = 10.53 PKK173 pKa = 10.1 AYY175 pKa = 10.19 DD176 pKa = 3.29 RR177 pKa = 11.84 GTRR180 pKa = 11.84 QYY182 pKa = 11.31 NLPFLPDD189 pKa = 3.41 HH190 pKa = 6.04 VLEE193 pKa = 4.68 TKK195 pKa = 10.42 AIYY198 pKa = 10.3 INGPAGSGKK207 pKa = 7.62 TRR209 pKa = 11.84 WSADD213 pKa = 3.0 HH214 pKa = 6.97 FEE216 pKa = 4.28 NPLLVSHH223 pKa = 7.19 KK224 pKa = 10.85 DD225 pKa = 3.41 DD226 pKa = 4.62 LKK228 pKa = 11.03 RR229 pKa = 11.84 LAKK232 pKa = 10.31 GSYY235 pKa = 9.85 NGIIFDD241 pKa = 5.19 DD242 pKa = 4.67 FNISHH247 pKa = 6.76 WPIGAVIHH255 pKa = 6.53 LCDD258 pKa = 4.74 LEE260 pKa = 4.15 WDD262 pKa = 3.5 RR263 pKa = 11.84 SIDD266 pKa = 3.79 VKK268 pKa = 10.91 HH269 pKa = 5.67 GTVTIPAQMPRR280 pKa = 11.84 IFTNNLSFDD289 pKa = 3.65 EE290 pKa = 4.48 WCPSGLTEE298 pKa = 4.02 EE299 pKa = 4.44 QKK301 pKa = 10.42 NALRR305 pKa = 11.84 RR306 pKa = 11.84 RR307 pKa = 11.84 IHH309 pKa = 6.32 VINVHH314 pKa = 4.93 VRR316 pKa = 11.84 LFEE319 pKa = 3.96
Molecular weight: 36.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.861
IPC2_protein 5.779
IPC_protein 5.855
Toseland 6.3
ProMoST 6.148
Dawson 6.16
Bjellqvist 6.16
Wikipedia 6.186
Rodwell 6.16
Grimsley 6.453
Solomon 6.16
Lehninger 6.16
Nozaki 6.44
DTASelect 6.634
Thurlkill 6.664
EMBOSS 6.62
Sillero 6.547
Patrickios 4.113
IPC_peptide 6.186
IPC2_peptide 6.547
IPC2.peptide.svr19 6.482
Protein with the highest isoelectric point:
>tr|A0A346BPE2|A0A346BPE2_9VIRU Putative capsid protein OS=Spider associated circular virus 3 OX=2293304 PE=4 SV=1
MM1 pKa = 7.69 GYY3 pKa = 10.66 GSKK6 pKa = 10.37 FYY8 pKa = 10.75 QKK10 pKa = 10.41 RR11 pKa = 11.84 KK12 pKa = 7.67 YY13 pKa = 10.16 QSPAYY18 pKa = 9.76 FGLSPYY24 pKa = 9.84 DD25 pKa = 3.3 WKK27 pKa = 11.13 RR28 pKa = 11.84 PTSGYY33 pKa = 10.4 YY34 pKa = 10.14 YY35 pKa = 10.59 GGFHH39 pKa = 6.68 IRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 VQRR46 pKa = 11.84 GAINSNYY53 pKa = 9.58 RR54 pKa = 11.84 LKK56 pKa = 10.23 STTKK60 pKa = 9.5 SYY62 pKa = 10.48 LRR64 pKa = 11.84 KK65 pKa = 9.36 RR66 pKa = 11.84 GRR68 pKa = 11.84 FVNHH72 pKa = 4.48 TRR74 pKa = 11.84 KK75 pKa = 10.46 GKK77 pKa = 9.33 FNRR80 pKa = 11.84 YY81 pKa = 7.67 HH82 pKa = 6.78 HH83 pKa = 6.25 ATSTGSRR90 pKa = 11.84 RR91 pKa = 11.84 FLAKK95 pKa = 10.34 RR96 pKa = 11.84 FPKK99 pKa = 10.15 RR100 pKa = 11.84 KK101 pKa = 9.29 AVRR104 pKa = 11.84 TITKK108 pKa = 10.14 RR109 pKa = 11.84 LVARR113 pKa = 11.84 QKK115 pKa = 10.12 PFPINKK121 pKa = 8.11 EE122 pKa = 3.93 ALGSDD127 pKa = 3.32 IKK129 pKa = 9.06 TTLLKK134 pKa = 10.42 SVPAGTVVPLDD145 pKa = 4.48 DD146 pKa = 4.56 YY147 pKa = 11.26 LIKK150 pKa = 11.11 YY151 pKa = 9.71 SDD153 pKa = 4.22 LGVSSGLQHH162 pKa = 7.36 DD163 pKa = 4.63 NKK165 pKa = 10.3 PLTWSFSTNLHH176 pKa = 4.58 VKK178 pKa = 10.32 YY179 pKa = 10.88 YY180 pKa = 9.65 LTFEE184 pKa = 4.2 FAGACIDD191 pKa = 3.94 LLVPQVPEE199 pKa = 3.74 KK200 pKa = 11.18 NIVKK204 pKa = 10.43 KK205 pKa = 11.0 LPDD208 pKa = 4.02 ALNSITKK215 pKa = 9.91 YY216 pKa = 11.02 ADD218 pKa = 2.98 VDD220 pKa = 3.91 EE221 pKa = 5.36 VDD223 pKa = 4.73 TLMQTCNII231 pKa = 4.16
Molecular weight: 26.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.838
IPC_protein 10.131
Toseland 10.379
ProMoST 10.043
Dawson 10.54
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 11.023
Grimsley 10.613
Solomon 10.57
Lehninger 10.54
Nozaki 10.35
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.672
IPC_peptide 10.57
IPC2_peptide 8.931
IPC2.peptide.svr19 8.712
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
550
231
319
275.0
31.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.818 ± 0.337
1.636 ± 0.417
6.364 ± 0.867
3.818 ± 1.129
4.364 ± 0.216
6.545 ± 0.028
3.636 ± 0.562
6.0 ± 0.904
6.909 ± 1.883
8.0 ± 0.356
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.727 ± 0.075
4.909 ± 0.314
5.273 ± 0.042
3.818 ± 0.426
7.273 ± 0.515
5.818 ± 0.834
7.091 ± 0.145
5.455 ± 0.328
1.818 ± 0.515
4.727 ± 1.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here