Spider associated circular virus 3

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346BPE2|A0A346BPE2_9VIRU Putative capsid protein OS=Spider associated circular virus 3 OX=2293304 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84TRR5 pKa = 11.84NWCFTIFTEE14 pKa = 5.48DD15 pKa = 3.37GDD17 pKa = 4.67GEE19 pKa = 4.32ASAHH23 pKa = 5.89YY24 pKa = 10.23DD25 pKa = 3.33PNTIDD30 pKa = 3.71WLSATPQVRR39 pKa = 11.84YY40 pKa = 9.92VVAGLEE46 pKa = 4.15VAPTSGRR53 pKa = 11.84LHH55 pKa = 5.05WQGYY59 pKa = 9.75IEE61 pKa = 4.5LQHH64 pKa = 6.69PATLVSCKK72 pKa = 10.39NSLGTDD78 pKa = 3.35KK79 pKa = 11.32AHH81 pKa = 7.11LEE83 pKa = 3.81IRR85 pKa = 11.84RR86 pKa = 11.84GTRR89 pKa = 11.84NQAIAYY95 pKa = 7.67CLKK98 pKa = 10.2QDD100 pKa = 3.7SAAQGEE106 pKa = 4.6PEE108 pKa = 4.22GNEE111 pKa = 3.67PKK113 pKa = 10.28IIFTWGDD120 pKa = 3.76DD121 pKa = 3.93NPQGTDD127 pKa = 3.47DD128 pKa = 4.64IYY130 pKa = 11.69DD131 pKa = 3.67EE132 pKa = 4.77VLCQTDD138 pKa = 3.74VNTAIQFIKK147 pKa = 10.24RR148 pKa = 11.84VRR150 pKa = 11.84PRR152 pKa = 11.84DD153 pKa = 3.43YY154 pKa = 10.97CIFGQQLHH162 pKa = 6.38TNLEE166 pKa = 4.02RR167 pKa = 11.84HH168 pKa = 4.47FTKK171 pKa = 10.53PKK173 pKa = 10.1AYY175 pKa = 10.19DD176 pKa = 3.29RR177 pKa = 11.84GTRR180 pKa = 11.84QYY182 pKa = 11.31NLPFLPDD189 pKa = 3.41HH190 pKa = 6.04VLEE193 pKa = 4.68TKK195 pKa = 10.42AIYY198 pKa = 10.3INGPAGSGKK207 pKa = 7.62TRR209 pKa = 11.84WSADD213 pKa = 3.0HH214 pKa = 6.97FEE216 pKa = 4.28NPLLVSHH223 pKa = 7.19KK224 pKa = 10.85DD225 pKa = 3.41DD226 pKa = 4.62LKK228 pKa = 11.03RR229 pKa = 11.84LAKK232 pKa = 10.31GSYY235 pKa = 9.85NGIIFDD241 pKa = 5.19DD242 pKa = 4.67FNISHH247 pKa = 6.76WPIGAVIHH255 pKa = 6.53LCDD258 pKa = 4.74LEE260 pKa = 4.15WDD262 pKa = 3.5RR263 pKa = 11.84SIDD266 pKa = 3.79VKK268 pKa = 10.91HH269 pKa = 5.67GTVTIPAQMPRR280 pKa = 11.84IFTNNLSFDD289 pKa = 3.65EE290 pKa = 4.48WCPSGLTEE298 pKa = 4.02EE299 pKa = 4.44QKK301 pKa = 10.42NALRR305 pKa = 11.84RR306 pKa = 11.84RR307 pKa = 11.84IHH309 pKa = 6.32VINVHH314 pKa = 4.93VRR316 pKa = 11.84LFEE319 pKa = 3.96

Molecular weight:
36.43 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346BPE2|A0A346BPE2_9VIRU Putative capsid protein OS=Spider associated circular virus 3 OX=2293304 PE=4 SV=1
MM1 pKa = 7.69GYY3 pKa = 10.66GSKK6 pKa = 10.37FYY8 pKa = 10.75QKK10 pKa = 10.41RR11 pKa = 11.84KK12 pKa = 7.67YY13 pKa = 10.16QSPAYY18 pKa = 9.76FGLSPYY24 pKa = 9.84DD25 pKa = 3.3WKK27 pKa = 11.13RR28 pKa = 11.84PTSGYY33 pKa = 10.4YY34 pKa = 10.14YY35 pKa = 10.59GGFHH39 pKa = 6.68IRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84VQRR46 pKa = 11.84GAINSNYY53 pKa = 9.58RR54 pKa = 11.84LKK56 pKa = 10.23STTKK60 pKa = 9.5SYY62 pKa = 10.48LRR64 pKa = 11.84KK65 pKa = 9.36RR66 pKa = 11.84GRR68 pKa = 11.84FVNHH72 pKa = 4.48TRR74 pKa = 11.84KK75 pKa = 10.46GKK77 pKa = 9.33FNRR80 pKa = 11.84YY81 pKa = 7.67HH82 pKa = 6.78HH83 pKa = 6.25ATSTGSRR90 pKa = 11.84RR91 pKa = 11.84FLAKK95 pKa = 10.34RR96 pKa = 11.84FPKK99 pKa = 10.15RR100 pKa = 11.84KK101 pKa = 9.29AVRR104 pKa = 11.84TITKK108 pKa = 10.14RR109 pKa = 11.84LVARR113 pKa = 11.84QKK115 pKa = 10.12PFPINKK121 pKa = 8.11EE122 pKa = 3.93ALGSDD127 pKa = 3.32IKK129 pKa = 9.06TTLLKK134 pKa = 10.42SVPAGTVVPLDD145 pKa = 4.48DD146 pKa = 4.56YY147 pKa = 11.26LIKK150 pKa = 11.11YY151 pKa = 9.71SDD153 pKa = 4.22LGVSSGLQHH162 pKa = 7.36DD163 pKa = 4.63NKK165 pKa = 10.3PLTWSFSTNLHH176 pKa = 4.58VKK178 pKa = 10.32YY179 pKa = 10.88YY180 pKa = 9.65LTFEE184 pKa = 4.2FAGACIDD191 pKa = 3.94LLVPQVPEE199 pKa = 3.74KK200 pKa = 11.18NIVKK204 pKa = 10.43KK205 pKa = 11.0LPDD208 pKa = 4.02ALNSITKK215 pKa = 9.91YY216 pKa = 11.02ADD218 pKa = 2.98VDD220 pKa = 3.91EE221 pKa = 5.36VDD223 pKa = 4.73TLMQTCNII231 pKa = 4.16

Molecular weight:
26.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

550

231

319

275.0

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.818 ± 0.337

1.636 ± 0.417

6.364 ± 0.867

3.818 ± 1.129

4.364 ± 0.216

6.545 ± 0.028

3.636 ± 0.562

6.0 ± 0.904

6.909 ± 1.883

8.0 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.727 ± 0.075

4.909 ± 0.314

5.273 ± 0.042

3.818 ± 0.426

7.273 ± 0.515

5.818 ± 0.834

7.091 ± 0.145

5.455 ± 0.328

1.818 ± 0.515

4.727 ± 1.19

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski