Stakelama pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Stakelama

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6FY44|A0A4R6FY44_9SPHN Xylan alpha-1 2-glucuronidase OS=Stakelama pacifica OX=517720 GN=EV664_101504 PE=3 SV=1
MM1 pKa = 7.82FDD3 pKa = 3.77AQQMASPYY11 pKa = 9.42VAFFRR16 pKa = 11.84EE17 pKa = 4.15PTTRR21 pKa = 11.84LDD23 pKa = 3.33RR24 pKa = 11.84FLVQGYY30 pKa = 7.9VDD32 pKa = 4.04RR33 pKa = 11.84AQLAAAVEE41 pKa = 4.32SLIEE45 pKa = 4.92LIDD48 pKa = 5.51AIDD51 pKa = 4.3GDD53 pKa = 4.47PDD55 pKa = 4.19FEE57 pKa = 4.36EE58 pKa = 4.5TDD60 pKa = 4.01CEE62 pKa = 4.21DD63 pKa = 3.41SHH65 pKa = 7.94VLSHH69 pKa = 7.38RR70 pKa = 11.84ALEE73 pKa = 4.33WASSPGCEE81 pKa = 3.88IADD84 pKa = 3.62AGGQHH89 pKa = 7.01DD90 pKa = 4.44EE91 pKa = 4.83DD92 pKa = 5.9GINTMFRR99 pKa = 11.84IDD101 pKa = 3.63EE102 pKa = 4.44TGAGCPIADD111 pKa = 4.62PDD113 pKa = 4.5CAVDD117 pKa = 5.27DD118 pKa = 4.26TACDD122 pKa = 3.4EE123 pKa = 4.54GEE125 pKa = 4.04AAA127 pKa = 5.51

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6FBW2|A0A4R6FBW2_9SPHN Putative lipid-binding transport protein (Tim44 family) OS=Stakelama pacifica OX=517720 GN=EV664_11615 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3799

0

3799

1198406

26

3299

315.5

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.971 ± 0.059

0.756 ± 0.012

6.265 ± 0.031

5.598 ± 0.041

3.515 ± 0.024

8.77 ± 0.039

2.031 ± 0.02

5.151 ± 0.028

3.053 ± 0.031

9.602 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.021

2.591 ± 0.025

5.25 ± 0.03

3.117 ± 0.02

7.55 ± 0.05

5.4 ± 0.029

5.225 ± 0.041

6.95 ± 0.034

1.429 ± 0.017

2.263 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski