Venturia nashicola
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11754 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z1P151|A0A4Z1P151_9PEZI VHS subgroup OS=Venturia nashicola OX=86259 GN=E6O75_ATG10297 PE=4 SV=1
MM1 pKa = 7.84 KK2 pKa = 9.83 STTLFTAATALFASTIANPVNSGSKK27 pKa = 8.68 STLSTADD34 pKa = 3.64 LQSVDD39 pKa = 4.0 TLLEE43 pKa = 4.25 SNKK46 pKa = 9.9 AFSDD50 pKa = 3.87 EE51 pKa = 4.13 AVVGHH56 pKa = 6.14 VKK58 pKa = 10.74 LPIDD62 pKa = 3.66 NSEE65 pKa = 4.65 TILSATPSAPLTGPTYY81 pKa = 11.04 GNSSHH86 pKa = 6.87 AGPRR90 pKa = 11.84 IWAPVCSKK98 pKa = 10.83 GCASTTTTTVIHH110 pKa = 6.38 VSTSTAVVSTMTVTAGVSSSPASTLSDD137 pKa = 3.54 YY138 pKa = 11.9 SNAADD143 pKa = 4.51 DD144 pKa = 4.58 SSTSSSDD151 pKa = 2.99 VDD153 pKa = 3.49 VGYY156 pKa = 7.52 MTSTQTLISTMTGANVTFSRR176 pKa = 11.84 STVTLAMNTTSSYY189 pKa = 10.79 TSLATVTIDD198 pKa = 3.23 PTNSVTFTLTTEE210 pKa = 4.05 TTMATTDD217 pKa = 3.18 TAEE220 pKa = 4.16 YY221 pKa = 10.74 DD222 pKa = 3.54 VTEE225 pKa = 4.27 TMDD228 pKa = 3.41 VASTISVDD236 pKa = 4.22 SVIDD240 pKa = 3.91 PDD242 pKa = 4.24 TATACANAIVDD253 pKa = 3.71 HH254 pKa = 6.35 AVNLTVDD261 pKa = 4.04 LTVDD265 pKa = 4.18 LDD267 pKa = 3.69 VCKK270 pKa = 10.58 ALDD273 pKa = 3.88 GVSIEE278 pKa = 4.14
Molecular weight: 28.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.554
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.279
Thurlkill 3.706
EMBOSS 3.846
Sillero 3.999
Patrickios 1.926
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A4Z1NPF6|A0A4Z1NPF6_9PEZI Glycoside hydrolase family 1 protein OS=Venturia nashicola OX=86259 GN=E6O75_ATG10541 PE=3 SV=1
MM1 pKa = 7.76 PKK3 pKa = 9.34 VQTYY7 pKa = 7.42 KK8 pKa = 10.69 TYY10 pKa = 9.84 IQPSIARR17 pKa = 11.84 GRR19 pKa = 11.84 SNSAAGRR26 pKa = 11.84 GHH28 pKa = 6.76 TSSTWRR34 pKa = 11.84 RR35 pKa = 11.84 FTRR38 pKa = 11.84 WRR40 pKa = 11.84 AVCAVNIGWKK50 pKa = 10.07 DD51 pKa = 3.2 RR52 pKa = 11.84 SSRR55 pKa = 11.84 EE56 pKa = 3.87 NILKK60 pKa = 10.09 LRR62 pKa = 11.84 QLPPNEE68 pKa = 4.56 TYY70 pKa = 10.85 NSSFSQRR77 pKa = 11.84 DD78 pKa = 3.68 NVCHH82 pKa = 6.45 EE83 pKa = 5.21 EE84 pKa = 3.95 IPVPDD89 pKa = 3.99 KK90 pKa = 11.56 SGDD93 pKa = 3.59 GPLVGFSVQLGTSLQVNINCGIGTLLGSCSSTEE126 pKa = 3.83 GHH128 pKa = 5.88 VRR130 pKa = 11.84 AGTQNQCMSQNTMPSLASKK149 pKa = 9.12 VRR151 pKa = 11.84 NWEE154 pKa = 3.75 LHH156 pKa = 5.54 LSSNSRR162 pKa = 11.84 GGFAQAFRR170 pKa = 11.84 FSRR173 pKa = 11.84 VRR175 pKa = 11.84 WLL177 pKa = 3.31
Molecular weight: 19.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.56
IPC_protein 10.379
Toseland 10.628
ProMoST 10.335
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.935
Rodwell 10.877
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.657
DTASelect 10.438
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.613
IPC_peptide 10.862
IPC2_peptide 9.78
IPC2.peptide.svr19 8.564
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11754
0
11754
5212399
66
9086
443.5
49.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.448 ± 0.021
1.194 ± 0.009
5.546 ± 0.015
6.257 ± 0.023
3.703 ± 0.016
6.894 ± 0.021
2.388 ± 0.01
4.995 ± 0.016
5.367 ± 0.022
8.607 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.271 ± 0.009
3.797 ± 0.013
6.145 ± 0.03
4.054 ± 0.019
5.913 ± 0.02
8.305 ± 0.026
6.113 ± 0.015
5.909 ± 0.016
1.426 ± 0.01
2.662 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here