Rattus norvegicus (Rat)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3ZDS3|D3ZDS3_RAT Tyrosine-protein kinase OS=Rattus norvegicus OX=10116 GN=Ptk6 PE=3 SV=1
MM1 pKa = 8.08DD2 pKa = 4.8PQAATGPGPGEE13 pKa = 3.77PSAWEE18 pKa = 4.08APSAEE23 pKa = 4.53AGLATADD30 pKa = 3.66LSGGEE35 pKa = 4.5TEE37 pKa = 4.68TEE39 pKa = 3.77LDD41 pKa = 3.29VDD43 pKa = 4.49RR44 pKa = 11.84LASGAQSIPTDD55 pKa = 3.58VPTHH59 pKa = 6.46AEE61 pKa = 4.42GPSSEE66 pKa = 4.33EE67 pKa = 3.39EE68 pKa = 4.01GFAMEE73 pKa = 5.0KK74 pKa = 10.34EE75 pKa = 4.27ADD77 pKa = 3.63GEE79 pKa = 4.32LYY81 pKa = 10.46AWEE84 pKa = 4.39LSEE87 pKa = 6.39GPACPPMEE95 pKa = 4.08QASGLFNEE103 pKa = 5.09DD104 pKa = 2.11WDD106 pKa = 4.88LEE108 pKa = 4.38LKK110 pKa = 10.49ADD112 pKa = 3.94QGNPYY117 pKa = 10.44DD118 pKa = 4.67ADD120 pKa = 3.96DD121 pKa = 3.96VQGSISQEE129 pKa = 3.43IKK131 pKa = 10.32PWVCCAPQGDD141 pKa = 4.19MMYY144 pKa = 10.5DD145 pKa = 4.02PSWHH149 pKa = 6.82HH150 pKa = 6.78PPPLIPHH157 pKa = 5.6YY158 pKa = 10.76SKK160 pKa = 10.49MVFEE164 pKa = 5.05TGQFDD169 pKa = 4.21DD170 pKa = 6.01AEE172 pKa = 4.35DD173 pKa = 3.31

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P84245|H33_RAT Histone H3.3 OS=Rattus norvegicus OX=10116 GN=H3-3b PE=1 SV=2
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21588

9974

31562

17292116

7

8031

547.9

61.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.829 ± 0.013

2.25 ± 0.013

4.864 ± 0.01

6.965 ± 0.018

3.761 ± 0.01

6.423 ± 0.017

2.577 ± 0.007

4.489 ± 0.013

5.726 ± 0.015

9.984 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.007

3.635 ± 0.01

6.046 ± 0.021

4.704 ± 0.015

5.561 ± 0.014

8.389 ± 0.02

5.463 ± 0.012

6.176 ± 0.013

1.198 ± 0.005

2.73 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski