Amino acid dipepetide frequency for Rattus norvegicus (Rat)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.441AlaAla: 6.441 ± 0.039	1.401AlaCys: 1.401 ± 0.012	2.888AlaAsp: 2.888 ± 0.015	4.658AlaGlu: 4.658 ± 0.031	2.727AlaPhe: 2.727 ± 0.017	4.483AlaGly: 4.483 ± 0.024	1.568AlaHis: 1.568 ± 0.012	2.91AlaIle: 2.91 ± 0.015	3.435AlaLys: 3.435 ± 0.021	7.09AlaLeu: 7.09 ± 0.027	1.54AlaMet: 1.54 ± 0.011	2.048AlaAsn: 2.048 ± 0.012	3.904AlaPro: 3.904 ± 0.023	3.154AlaGln: 3.154 ± 0.02	3.51AlaArg: 3.51 ± 0.017	5.794AlaSer: 5.794 ± 0.026	3.638AlaThr: 3.638 ± 0.02	4.753AlaVal: 4.753 ± 0.02	0.768AlaTrp: 0.768 ± 0.008	1.578AlaTyr: 1.578 ± 0.011	0.003AlaXaa: 0.003 ± 0.0
Cys	1.287CysAla: 1.287 ± 0.012	0.688CysCys: 0.688 ± 0.014	1.067CysAsp: 1.067 ± 0.012	1.289CysGlu: 1.289 ± 0.016	0.891CysPhe: 0.891 ± 0.009	1.746CysGly: 1.746 ± 0.019	0.653CysHis: 0.653 ± 0.008	0.995CysIle: 0.995 ± 0.008	1.175CysLys: 1.175 ± 0.013	2.22CysLeu: 2.22 ± 0.015	0.453CysMet: 0.453 ± 0.005	0.796CysAsn: 0.796 ± 0.009	1.353CysPro: 1.353 ± 0.016	1.088CysGln: 1.088 ± 0.012	1.29CysArg: 1.29 ± 0.012	2.078CysSer: 2.078 ± 0.015	1.149CysThr: 1.149 ± 0.01	1.379CysVal: 1.379 ± 0.012	0.307CysTrp: 0.307 ± 0.005	0.599CysTyr: 0.599 ± 0.006	0.001CysXaa: 0.001 ± 0.0
Asp	2.829AspAla: 2.829 ± 0.016	1.064AspCys: 1.064 ± 0.01	2.631AspAsp: 2.631 ± 0.018	3.311AspGlu: 3.311 ± 0.017	2.128AspPhe: 2.128 ± 0.013	3.312AspGly: 3.312 ± 0.02	1.125AspHis: 1.125 ± 0.008	2.63AspIle: 2.63 ± 0.017	2.491AspLys: 2.491 ± 0.014	4.991AspLeu: 4.991 ± 0.022	1.149AspMet: 1.149 ± 0.008	1.691AspAsn: 1.691 ± 0.011	2.921AspPro: 2.921 ± 0.014	1.827AspGln: 1.827 ± 0.011	2.523AspArg: 2.523 ± 0.015	4.268AspSer: 4.268 ± 0.021	2.548AspThr: 2.548 ± 0.015	3.099AspVal: 3.099 ± 0.028	0.627AspTrp: 0.627 ± 0.007	1.475AspTyr: 1.475 ± 0.011	0.002AspXaa: 0.002 ± 0.0
Glu	5.157GluAla: 5.157 ± 0.031	1.34GluCys: 1.34 ± 0.016	4.452GluAsp: 4.452 ± 0.02	7.766GluGlu: 7.766 ± 0.05	2.018GluPhe: 2.018 ± 0.012	4.135GluGly: 4.135 ± 0.023	1.491GluHis: 1.491 ± 0.01	3.0GluIle: 3.0 ± 0.017	5.153GluLys: 5.153 ± 0.032	6.292GluLeu: 6.292 ± 0.034	1.626GluMet: 1.626 ± 0.011	2.981GluAsn: 2.981 ± 0.015	3.169GluPro: 3.169 ± 0.021	3.072GluGln: 3.072 ± 0.02	3.893GluArg: 3.893 ± 0.024	4.329GluSer: 4.329 ± 0.022	3.42GluThr: 3.42 ± 0.017	4.124GluVal: 4.124 ± 0.023	0.687GluTrp: 0.687 ± 0.007	1.548GluTyr: 1.548 ± 0.012	0.002GluXaa: 0.002 ± 0.0
Phe	2.028PheAla: 2.028 ± 0.012	1.05PheCys: 1.05 ± 0.01	1.668PheAsp: 1.668 ± 0.01	1.964PheGlu: 1.964 ± 0.012	1.777PhePhe: 1.777 ± 0.015	2.264PheGly: 2.264 ± 0.016	1.028PheHis: 1.028 ± 0.008	2.01PheIle: 2.01 ± 0.015	1.773PheLys: 1.773 ± 0.014	4.4PheLeu: 4.4 ± 0.023	0.825PheMet: 0.825 ± 0.008	1.375PheAsn: 1.375 ± 0.009	2.005PhePro: 2.005 ± 0.011	1.791PheGln: 1.791 ± 0.012	1.975PheArg: 1.975 ± 0.012	3.5PheSer: 3.5 ± 0.016	2.117PheThr: 2.117 ± 0.012	2.264PheVal: 2.264 ± 0.013	0.514PheTrp: 0.514 ± 0.007	1.286PheTyr: 1.286 ± 0.01	0.001PheXaa: 0.001 ± 0.0
Gly	4.334GlyAla: 4.334 ± 0.023	1.346GlyCys: 1.346 ± 0.014	3.168GlyAsp: 3.168 ± 0.016	4.014GlyGlu: 4.014 ± 0.022	2.48GlyPhe: 2.48 ± 0.015	4.92GlyGly: 4.92 ± 0.04	1.699GlyHis: 1.699 ± 0.011	2.785GlyIle: 2.785 ± 0.016	3.772GlyLys: 3.772 ± 0.019	5.909GlyLeu: 5.909 ± 0.024	1.309GlyMet: 1.309 ± 0.01	2.435GlyAsn: 2.435 ± 0.015	4.075GlyPro: 4.075 ± 0.047	2.726GlyGln: 2.726 ± 0.015	3.603GlyArg: 3.603 ± 0.02	5.83GlySer: 5.83 ± 0.031	3.677GlyThr: 3.677 ± 0.021	3.625GlyVal: 3.625 ± 0.018	0.766GlyTrp: 0.766 ± 0.008	1.753GlyTyr: 1.753 ± 0.012	0.002GlyXaa: 0.002 ± 0.0
His	1.301HisAla: 1.301 ± 0.009	0.73HisCys: 0.73 ± 0.009	0.87HisAsp: 0.87 ± 0.008	1.314HisGlu: 1.314 ± 0.01	1.105HisPhe: 1.105 ± 0.009	1.559HisGly: 1.559 ± 0.012	0.887HisHis: 0.887 ± 0.011	1.266HisIle: 1.266 ± 0.01	1.26HisLys: 1.26 ± 0.01	2.901HisLeu: 2.901 ± 0.017	0.593HisMet: 0.593 ± 0.007	0.854HisAsn: 0.854 ± 0.007	1.613HisPro: 1.613 ± 0.011	1.284HisGln: 1.284 ± 0.013	1.614HisArg: 1.614 ± 0.011	2.325HisSer: 2.325 ± 0.014	1.638HisThr: 1.638 ± 0.021	1.478HisVal: 1.478 ± 0.01	0.353HisTrp: 0.353 ± 0.005	0.826HisTyr: 0.826 ± 0.01	0.001HisXaa: 0.001 ± 0.0
Ile	2.607IleAla: 2.607 ± 0.014	1.145IleCys: 1.145 ± 0.009	2.023IleAsp: 2.023 ± 0.015	2.498IleGlu: 2.498 ± 0.016	2.021IlePhe: 2.021 ± 0.016	2.287IleGly: 2.287 ± 0.014	1.358IleHis: 1.358 ± 0.012	2.525IleIle: 2.525 ± 0.016	2.524IleLys: 2.524 ± 0.017	4.818IleLeu: 4.818 ± 0.021	1.048IleMet: 1.048 ± 0.008	1.824IleAsn: 1.824 ± 0.012	2.639IlePro: 2.639 ± 0.014	2.248IleGln: 2.248 ± 0.013	2.36IleArg: 2.36 ± 0.014	3.738IleSer: 3.738 ± 0.018	2.602IleThr: 2.602 ± 0.014	2.602IleVal: 2.602 ± 0.013	0.529IleTrp: 0.529 ± 0.006	1.495IleTyr: 1.495 ± 0.011	0.001IleXaa: 0.001 ± 0.0
Lys	4.093LysAla: 4.093 ± 0.022	1.137LysCys: 1.137 ± 0.011	3.16LysAsp: 3.16 ± 0.016	4.988LysGlu: 4.988 ± 0.03	1.754LysPhe: 1.754 ± 0.012	3.309LysGly: 3.309 ± 0.027	1.396LysHis: 1.396 ± 0.011	2.675LysIle: 2.675 ± 0.015	4.577LysLys: 4.577 ± 0.029	5.025LysLeu: 5.025 ± 0.026	1.446LysMet: 1.446 ± 0.015	2.295LysAsn: 2.295 ± 0.014	3.082LysPro: 3.082 ± 0.021	2.54LysGln: 2.54 ± 0.015	3.231LysArg: 3.231 ± 0.016	3.857LysSer: 3.857 ± 0.02	3.104LysThr: 3.104 ± 0.017	3.466LysVal: 3.466 ± 0.02	0.591LysTrp: 0.591 ± 0.006	1.532LysTyr: 1.532 ± 0.012	0.001LysXaa: 0.001 ± 0.0
Leu	6.849LeuAla: 6.849 ± 0.025	2.243LeuCys: 2.243 ± 0.015	4.744LeuAsp: 4.744 ± 0.022	6.92LeuGlu: 6.92 ± 0.037	3.575LeuPhe: 3.575 ± 0.02	5.941LeuGly: 5.941 ± 0.023	2.732LeuHis: 2.732 ± 0.015	4.021LeuIle: 4.021 ± 0.017	5.734LeuLys: 5.734 ± 0.031	11.061LeuLeu: 11.061 ± 0.047	2.091LeuMet: 2.091 ± 0.012	3.514LeuAsn: 3.514 ± 0.018	5.876LeuPro: 5.876 ± 0.024	5.677LeuGln: 5.677 ± 0.03	6.019LeuArg: 6.019 ± 0.026	8.188LeuSer: 8.188 ± 0.029	5.198LeuThr: 5.198 ± 0.019	5.708LeuVal: 5.708 ± 0.025	1.154LeuTrp: 1.154 ± 0.01	2.591LeuTyr: 2.591 ± 0.015	0.004LeuXaa: 0.004 ± 0.0
Met	1.998MetAla: 1.998 ± 0.012	0.404MetCys: 0.404 ± 0.006	1.274MetAsp: 1.274 ± 0.008	1.833MetGlu: 1.833 ± 0.012	0.756MetPhe: 0.756 ± 0.008	1.329MetGly: 1.329 ± 0.01	0.459MetHis: 0.459 ± 0.006	0.869MetIle: 0.869 ± 0.007	1.449MetLys: 1.449 ± 0.011	2.073MetLeu: 2.073 ± 0.011	0.587MetMet: 0.587 ± 0.006	0.92MetAsn: 0.92 ± 0.008	1.107MetPro: 1.107 ± 0.017	0.906MetGln: 0.906 ± 0.007	1.044MetArg: 1.044 ± 0.009	1.667MetSer: 1.667 ± 0.01	1.171MetThr: 1.171 ± 0.009	1.463MetVal: 1.463 ± 0.01	0.246MetTrp: 0.246 ± 0.004	0.673MetTyr: 0.673 ± 0.006	0.001MetXaa: 0.001 ± 0.0
Asn	2.042AsnAla: 2.042 ± 0.013	0.84AsnCys: 0.84 ± 0.009	1.493AsnAsp: 1.493 ± 0.01	2.09AsnGlu: 2.09 ± 0.012	1.512AsnPhe: 1.512 ± 0.012	2.465AsnGly: 2.465 ± 0.019	0.959AsnHis: 0.959 ± 0.008	2.102AsnIle: 2.102 ± 0.013	2.14AsnLys: 2.14 ± 0.012	3.712AsnLeu: 3.712 ± 0.021	0.9AsnMet: 0.9 ± 0.007	1.486AsnAsn: 1.486 ± 0.015	2.243AsnPro: 2.243 ± 0.015	1.611AsnGln: 1.611 ± 0.011	1.861AsnArg: 1.861 ± 0.012	3.113AsnSer: 3.113 ± 0.016	1.991AsnThr: 1.991 ± 0.013	2.22AsnVal: 2.22 ± 0.014	0.457AsnTrp: 0.457 ± 0.005	1.11AsnTyr: 1.11 ± 0.008	0.001AsnXaa: 0.001 ± 0.0
Pro	4.563ProAla: 4.563 ± 0.028	1.173ProCys: 1.173 ± 0.016	2.759ProAsp: 2.759 ± 0.02	4.389ProGlu: 4.389 ± 0.024	1.929ProPhe: 1.929 ± 0.012	5.023ProGly: 5.023 ± 0.046	1.403ProHis: 1.403 ± 0.011	1.877ProIle: 1.877 ± 0.013	2.823ProLys: 2.823 ± 0.032	5.161ProLeu: 5.161 ± 0.021	1.135ProMet: 1.135 ± 0.01	1.822ProAsn: 1.822 ± 0.012	5.66ProPro: 5.66 ± 0.049	2.78ProGln: 2.78 ± 0.018	3.2ProArg: 3.2 ± 0.021	5.633ProSer: 5.633 ± 0.029	3.125ProThr: 3.125 ± 0.019	3.824ProVal: 3.824 ± 0.018	0.673ProTrp: 0.673 ± 0.007	1.502ProTyr: 1.502 ± 0.012	0.002ProXaa: 0.002 ± 0.0
Gln	3.412GlnAla: 3.412 ± 0.022	0.97GlnCys: 0.97 ± 0.011	2.336GlnAsp: 2.336 ± 0.013	3.803GlnGlu: 3.803 ± 0.024	1.353GlnPhe: 1.353 ± 0.01	2.829GlnGly: 2.829 ± 0.019	1.241GlnHis: 1.241 ± 0.01	1.934GlnIle: 1.934 ± 0.011	2.904GlnLys: 2.904 ± 0.015	4.647GlnLeu: 4.647 ± 0.025	1.12GlnMet: 1.12 ± 0.008	1.784GlnAsn: 1.784 ± 0.012	2.748GlnPro: 2.748 ± 0.021	3.033GlnGln: 3.033 ± 0.034	2.911GlnArg: 2.911 ± 0.021	3.153GlnSer: 3.153 ± 0.017	2.358GlnThr: 2.358 ± 0.013	2.825GlnVal: 2.825 ± 0.014	0.537GlnTrp: 0.537 ± 0.006	1.135GlnTyr: 1.135 ± 0.009	0.002GlnXaa: 0.002 ± 0.0
Arg	3.758ArgAla: 3.758 ± 0.02	1.198ArgCys: 1.198 ± 0.012	2.765ArgAsp: 2.765 ± 0.015	3.869ArgGlu: 3.869 ± 0.023	1.872ArgPhe: 1.872 ± 0.012	3.481ArgGly: 3.481 ± 0.022	1.526ArgHis: 1.526 ± 0.011	2.451ArgIle: 2.451 ± 0.013	3.647ArgLys: 3.647 ± 0.019	5.342ArgLeu: 5.342 ± 0.025	1.21ArgMet: 1.21 ± 0.01	2.179ArgAsn: 2.179 ± 0.013	3.089ArgPro: 3.089 ± 0.018	2.613ArgGln: 2.613 ± 0.016	4.207ArgArg: 4.207 ± 0.026	4.226ArgSer: 4.226 ± 0.025	2.829ArgThr: 2.829 ± 0.013	3.184ArgVal: 3.184 ± 0.014	0.679ArgTrp: 0.679 ± 0.008	1.489ArgTyr: 1.489 ± 0.009	0.002ArgXaa: 0.002 ± 0.0
Ser	5.277SerAla: 5.277 ± 0.022	1.92SerCys: 1.92 ± 0.019	3.886SerAsp: 3.886 ± 0.02	5.117SerGlu: 5.117 ± 0.022	3.14SerPhe: 3.14 ± 0.014	5.676SerGly: 5.676 ± 0.031	2.185SerHis: 2.185 ± 0.013	3.307SerIle: 3.307 ± 0.017	4.064SerLys: 4.064 ± 0.023	8.347SerLeu: 8.347 ± 0.027	1.666SerMet: 1.666 ± 0.013	2.706SerAsn: 2.706 ± 0.019	5.878SerPro: 5.878 ± 0.034	3.872SerGln: 3.872 ± 0.019	4.507SerArg: 4.507 ± 0.024	9.328SerSer: 9.328 ± 0.05	4.657SerThr: 4.657 ± 0.031	5.082SerVal: 5.082 ± 0.021	1.079SerTrp: 1.079 ± 0.01	2.196SerTyr: 2.196 ± 0.012	0.003SerXaa: 0.003 ± 0.0
Thr	3.791ThrAla: 3.791 ± 0.019	1.379ThrCys: 1.379 ± 0.014	2.489ThrAsp: 2.489 ± 0.014	3.631ThrGlu: 3.631 ± 0.019	2.216ThrPhe: 2.216 ± 0.014	3.616ThrGly: 3.616 ± 0.019	1.421ThrHis: 1.421 ± 0.017	2.48ThrIle: 2.48 ± 0.017	2.679ThrLys: 2.679 ± 0.015	5.486ThrLeu: 5.486 ± 0.023	1.184ThrMet: 1.184 ± 0.008	1.73ThrAsn: 1.73 ± 0.011	3.576ThrPro: 3.576 ± 0.023	2.344ThrGln: 2.344 ± 0.013	2.513ThrArg: 2.513 ± 0.012	4.647ThrSer: 4.647 ± 0.034	3.147ThrThr: 3.147 ± 0.049	4.086ThrVal: 4.086 ± 0.018	0.705ThrTrp: 0.705 ± 0.007	1.511ThrTyr: 1.511 ± 0.012	0.002ThrXaa: 0.002 ± 0.0
Val	4.315ValAla: 4.315 ± 0.018	1.538ValCys: 1.538 ± 0.012	3.009ValAsp: 3.009 ± 0.023	3.799ValGlu: 3.799 ± 0.021	2.566ValPhe: 2.566 ± 0.016	3.44ValGly: 3.44 ± 0.015	1.566ValHis: 1.566 ± 0.012	3.041ValIle: 3.041 ± 0.015	3.402ValLys: 3.402 ± 0.018	6.382ValLeu: 6.382 ± 0.028	1.388ValMet: 1.388 ± 0.009	2.266ValAsn: 2.266 ± 0.014	3.674ValPro: 3.674 ± 0.019	2.727ValGln: 2.727 ± 0.013	3.051ValArg: 3.051 ± 0.014	5.019ValSer: 5.019 ± 0.023	3.969ValThr: 3.969 ± 0.021	4.185ValVal: 4.185 ± 0.019	0.73ValTrp: 0.73 ± 0.008	1.686ValTyr: 1.686 ± 0.011	0.002ValXaa: 0.002 ± 0.0
Trp	0.777TrpAla: 0.777 ± 0.009	0.239TrpCys: 0.239 ± 0.004	0.647TrpAsp: 0.647 ± 0.007	0.772TrpGlu: 0.772 ± 0.007	0.466TrpPhe: 0.466 ± 0.006	0.702TrpGly: 0.702 ± 0.008	0.302TrpHis: 0.302 ± 0.004	0.588TrpIle: 0.588 ± 0.007	0.804TrpLys: 0.804 ± 0.009	1.231TrpLeu: 1.231 ± 0.011	0.317TrpMet: 0.317 ± 0.004	0.543TrpAsn: 0.543 ± 0.007	0.507TrpPro: 0.507 ± 0.006	0.518TrpGln: 0.518 ± 0.007	0.711TrpArg: 0.711 ± 0.007	0.886TrpSer: 0.886 ± 0.009	0.689TrpThr: 0.689 ± 0.008	0.73TrpVal: 0.73 ± 0.008	0.201TrpTrp: 0.201 ± 0.004	0.351TrpTyr: 0.351 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.426TyrAla: 1.426 ± 0.01	0.696TyrCys: 0.696 ± 0.008	1.313TyrAsp: 1.313 ± 0.01	1.627TyrGlu: 1.627 ± 0.011	1.327TyrPhe: 1.327 ± 0.01	1.713TyrGly: 1.713 ± 0.013	0.77TyrHis: 0.77 ± 0.007	1.428TyrIle: 1.428 ± 0.012	1.449TyrLys: 1.449 ± 0.013	2.75TyrLeu: 2.75 ± 0.016	0.643TyrMet: 0.643 ± 0.006	1.098TyrAsn: 1.098 ± 0.009	1.334TyrPro: 1.334 ± 0.009	1.225TyrGln: 1.225 ± 0.009	1.589TyrArg: 1.589 ± 0.011	2.308TyrSer: 2.308 ± 0.014	1.603TyrThr: 1.603 ± 0.012	1.659TyrVal: 1.659 ± 0.011	0.376TyrTrp: 0.376 ± 0.005	0.963TyrTyr: 0.963 ± 0.008	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.003XaaGly: 0.003 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.002XaaLeu: 0.002 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.003XaaPro: 0.003 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.023XaaXaa: 0.023 ± 0.004

Statistics based on 31562 proteins (17292116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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