Vibrio phage phi 2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067ZIB4|A0A067ZIB4_9CAUD Uncharacterized protein OS=Vibrio phage phi 2 OX=1476902 GN=Phi2_0067 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 2.95KK4 pKa = 11.08LKK6 pKa = 11.11NFVVNYY12 pKa = 7.65TLSNGVIGDD21 pKa = 3.86YY22 pKa = 10.69EE23 pKa = 4.3AVSPSSTLAALDD35 pKa = 4.03FCQFAYY41 pKa = 9.19EE42 pKa = 3.98NKK44 pKa = 9.02MAVSSISIDD53 pKa = 3.44EE54 pKa = 4.39AEE56 pKa = 4.54EE57 pKa = 4.33DD58 pKa = 4.22GEE60 pKa = 4.29

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067ZG47|A0A067ZG47_9CAUD Late control D family protein OS=Vibrio phage phi 2 OX=1476902 GN=Phi2_0025 PE=4 SV=1
MM1 pKa = 6.8AAEE4 pKa = 5.42WITFDD9 pKa = 5.78DD10 pKa = 3.98SQKK13 pKa = 10.43QRR15 pKa = 11.84AEE17 pKa = 3.91LLLAGIRR24 pKa = 11.84DD25 pKa = 4.16GASKK29 pKa = 10.42AASRR33 pKa = 11.84AINRR37 pKa = 11.84AILTARR43 pKa = 11.84SEE45 pKa = 4.77SINKK49 pKa = 8.91ARR51 pKa = 11.84EE52 pKa = 3.91EE53 pKa = 4.31YY54 pKa = 9.27TVKK57 pKa = 10.9AKK59 pKa = 10.37DD60 pKa = 3.3VRR62 pKa = 11.84EE63 pKa = 3.87TLKK66 pKa = 10.51ISKK69 pKa = 7.87ATPRR73 pKa = 11.84RR74 pKa = 11.84PIAVISSLGAPLPLSSFQVSPKK96 pKa = 8.96TVNGKK101 pKa = 9.09RR102 pKa = 11.84RR103 pKa = 11.84SAIRR107 pKa = 11.84VSVKK111 pKa = 10.3KK112 pKa = 10.59GGRR115 pKa = 11.84QSFDD119 pKa = 2.77RR120 pKa = 11.84AFIARR125 pKa = 11.84PSGQIDD131 pKa = 3.73VYY133 pKa = 10.96EE134 pKa = 4.14RR135 pKa = 11.84AGKK138 pKa = 9.92KK139 pKa = 9.57RR140 pKa = 11.84LPIRR144 pKa = 11.84KK145 pKa = 8.63LFGPSVPQMVGNPKK159 pKa = 10.08VISHH163 pKa = 7.11IADD166 pKa = 3.68KK167 pKa = 10.81ARR169 pKa = 11.84EE170 pKa = 4.04MMDD173 pKa = 2.83KK174 pKa = 10.73RR175 pKa = 11.84LDD177 pKa = 3.66HH178 pKa = 6.9EE179 pKa = 4.47IGRR182 pKa = 11.84LLDD185 pKa = 3.6GARR188 pKa = 3.68

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12878

48

878

192.2

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.247 ± 0.685

1.095 ± 0.178

6.134 ± 0.329

6.958 ± 0.376

3.735 ± 0.17

6.748 ± 0.311

1.522 ± 0.171

6.375 ± 0.283

7.369 ± 0.418

8.239 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.155

4.527 ± 0.241

4.1 ± 0.296

4.333 ± 0.284

4.946 ± 0.232

6.709 ± 0.412

5.342 ± 0.287

6.538 ± 0.347

1.522 ± 0.123

2.997 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski