Vibrio phage phi 2
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067ZIB4|A0A067ZIB4_9CAUD Uncharacterized protein OS=Vibrio phage phi 2 OX=1476902 GN=Phi2_0067 PE=4 SV=1
MM1 pKa = 7.74 SDD3 pKa = 2.95 KK4 pKa = 11.08 LKK6 pKa = 11.11 NFVVNYY12 pKa = 7.65 TLSNGVIGDD21 pKa = 3.86 YY22 pKa = 10.69 EE23 pKa = 4.3 AVSPSSTLAALDD35 pKa = 4.03 FCQFAYY41 pKa = 9.19 EE42 pKa = 3.98 NKK44 pKa = 9.02 MAVSSISIDD53 pKa = 3.44 EE54 pKa = 4.39 AEE56 pKa = 4.54 EE57 pKa = 4.33 DD58 pKa = 4.22 GEE60 pKa = 4.29
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.872
IPC_protein 3.732
Toseland 3.554
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.986
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.643
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A067ZG47|A0A067ZG47_9CAUD Late control D family protein OS=Vibrio phage phi 2 OX=1476902 GN=Phi2_0025 PE=4 SV=1
MM1 pKa = 6.8 AAEE4 pKa = 5.42 WITFDD9 pKa = 5.78 DD10 pKa = 3.98 SQKK13 pKa = 10.43 QRR15 pKa = 11.84 AEE17 pKa = 3.91 LLLAGIRR24 pKa = 11.84 DD25 pKa = 4.16 GASKK29 pKa = 10.42 AASRR33 pKa = 11.84 AINRR37 pKa = 11.84 AILTARR43 pKa = 11.84 SEE45 pKa = 4.77 SINKK49 pKa = 8.91 ARR51 pKa = 11.84 EE52 pKa = 3.91 EE53 pKa = 4.31 YY54 pKa = 9.27 TVKK57 pKa = 10.9 AKK59 pKa = 10.37 DD60 pKa = 3.3 VRR62 pKa = 11.84 EE63 pKa = 3.87 TLKK66 pKa = 10.51 ISKK69 pKa = 7.87 ATPRR73 pKa = 11.84 RR74 pKa = 11.84 PIAVISSLGAPLPLSSFQVSPKK96 pKa = 8.96 TVNGKK101 pKa = 9.09 RR102 pKa = 11.84 RR103 pKa = 11.84 SAIRR107 pKa = 11.84 VSVKK111 pKa = 10.3 KK112 pKa = 10.59 GGRR115 pKa = 11.84 QSFDD119 pKa = 2.77 RR120 pKa = 11.84 AFIARR125 pKa = 11.84 PSGQIDD131 pKa = 3.73 VYY133 pKa = 10.96 EE134 pKa = 4.14 RR135 pKa = 11.84 AGKK138 pKa = 9.92 KK139 pKa = 9.57 RR140 pKa = 11.84 LPIRR144 pKa = 11.84 KK145 pKa = 8.63 LFGPSVPQMVGNPKK159 pKa = 10.08 VISHH163 pKa = 7.11 IADD166 pKa = 3.68 KK167 pKa = 10.81 ARR169 pKa = 11.84 EE170 pKa = 4.04 MMDD173 pKa = 2.83 KK174 pKa = 10.73 RR175 pKa = 11.84 LDD177 pKa = 3.66 HH178 pKa = 6.9 EE179 pKa = 4.47 IGRR182 pKa = 11.84 LLDD185 pKa = 3.6 GARR188 pKa = 3.68
Molecular weight: 20.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.78
IPC_protein 10.716
Toseland 11.169
ProMoST 11.272
Dawson 11.199
Bjellqvist 10.965
Wikipedia 11.477
Rodwell 11.389
Grimsley 11.228
Solomon 11.447
Lehninger 11.403
Nozaki 11.14
DTASelect 10.965
Thurlkill 11.14
EMBOSS 11.594
Sillero 11.155
Patrickios 11.111
IPC_peptide 11.462
IPC2_peptide 9.926
IPC2.peptide.svr19 8.934
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
12878
48
878
192.2
21.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.247 ± 0.685
1.095 ± 0.178
6.134 ± 0.329
6.958 ± 0.376
3.735 ± 0.17
6.748 ± 0.311
1.522 ± 0.171
6.375 ± 0.283
7.369 ± 0.418
8.239 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.563 ± 0.155
4.527 ± 0.241
4.1 ± 0.296
4.333 ± 0.284
4.946 ± 0.232
6.709 ± 0.412
5.342 ± 0.287
6.538 ± 0.347
1.522 ± 0.123
2.997 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here