Amino acid dipepetide frequency for Vibrio phage phi 2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.018AlaAla: 10.018 ± 1.805
0.777AlaCys: 0.777 ± 0.292
4.97AlaAsp: 4.97 ± 0.405
6.368AlaGlu: 6.368 ± 0.605
2.407AlaPhe: 2.407 ± 0.362
5.203AlaGly: 5.203 ± 0.791
0.311AlaHis: 0.311 ± 0.198
4.271AlaIle: 4.271 ± 0.588
6.523AlaLys: 6.523 ± 0.798
6.756AlaLeu: 6.756 ± 0.742
2.951AlaMet: 2.951 ± 0.669
3.883AlaAsn: 3.883 ± 0.657
2.64AlaPro: 2.64 ± 0.437
2.718AlaGln: 2.718 ± 0.786
3.805AlaArg: 3.805 ± 0.553
5.902AlaSer: 5.902 ± 0.805
4.271AlaThr: 4.271 ± 0.689
5.281AlaVal: 5.281 ± 0.675
1.553AlaTrp: 1.553 ± 0.307
1.786AlaTyr: 1.786 ± 0.304
0.0AlaXaa: 0.0 ± 0.0
Cys
1.01CysAla: 1.01 ± 0.269
0.233CysCys: 0.233 ± 0.136
0.777CysAsp: 0.777 ± 0.252
0.699CysGlu: 0.699 ± 0.219
0.621CysPhe: 0.621 ± 0.219
1.087CysGly: 1.087 ± 0.264
0.078CysHis: 0.078 ± 0.08
0.311CysIle: 0.311 ± 0.153
0.932CysLys: 0.932 ± 0.279
0.777CysLeu: 0.777 ± 0.219
0.311CysMet: 0.311 ± 0.149
0.466CysAsn: 0.466 ± 0.193
0.699CysPro: 0.699 ± 0.227
0.699CysGln: 0.699 ± 0.233
0.544CysArg: 0.544 ± 0.179
0.621CysSer: 0.621 ± 0.249
0.466CysThr: 0.466 ± 0.182
0.388CysVal: 0.388 ± 0.182
0.0CysTrp: 0.0 ± 0.0
0.233CysTyr: 0.233 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
3.961AspAla: 3.961 ± 0.501
0.544AspCys: 0.544 ± 0.208
3.961AspAsp: 3.961 ± 0.561
4.504AspGlu: 4.504 ± 0.519
2.563AspPhe: 2.563 ± 0.497
4.582AspGly: 4.582 ± 0.623
1.243AspHis: 1.243 ± 0.291
3.805AspIle: 3.805 ± 0.579
4.038AspLys: 4.038 ± 0.563
6.135AspLeu: 6.135 ± 0.61
1.165AspMet: 1.165 ± 0.327
2.407AspAsn: 2.407 ± 0.496
3.184AspPro: 3.184 ± 0.568
3.339AspGln: 3.339 ± 0.529
2.64AspArg: 2.64 ± 0.512
4.349AspSer: 4.349 ± 0.55
1.631AspThr: 1.631 ± 0.329
3.961AspVal: 3.961 ± 0.567
1.475AspTrp: 1.475 ± 0.325
1.864AspTyr: 1.864 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
5.436GluAla: 5.436 ± 0.702
0.932GluCys: 0.932 ± 0.271
3.184GluAsp: 3.184 ± 0.456
3.728GluGlu: 3.728 ± 0.526
3.495GluPhe: 3.495 ± 0.48
3.883GluGly: 3.883 ± 0.567
0.777GluHis: 0.777 ± 0.205
5.281GluIle: 5.281 ± 0.729
3.883GluLys: 3.883 ± 0.744
6.834GluLeu: 6.834 ± 0.779
2.174GluMet: 2.174 ± 0.4
2.873GluAsn: 2.873 ± 0.367
2.563GluPro: 2.563 ± 0.551
3.728GluGln: 3.728 ± 0.472
3.728GluArg: 3.728 ± 0.468
4.659GluSer: 4.659 ± 0.534
3.262GluThr: 3.262 ± 0.445
4.892GluVal: 4.892 ± 0.842
1.553GluTrp: 1.553 ± 0.365
2.718GluTyr: 2.718 ± 0.358
0.0GluXaa: 0.0 ± 0.0
Phe
2.485PheAla: 2.485 ± 0.344
1.01PheCys: 1.01 ± 0.308
2.873PheAsp: 2.873 ± 0.363
3.106PheGlu: 3.106 ± 0.434
1.165PhePhe: 1.165 ± 0.253
2.873PheGly: 2.873 ± 0.307
0.466PheHis: 0.466 ± 0.254
2.252PheIle: 2.252 ± 0.537
2.873PheLys: 2.873 ± 0.469
2.796PheLeu: 2.796 ± 0.424
1.087PheMet: 1.087 ± 0.245
1.786PheAsn: 1.786 ± 0.371
0.699PhePro: 0.699 ± 0.206
0.854PheGln: 0.854 ± 0.2
1.087PheArg: 1.087 ± 0.295
3.495PheSer: 3.495 ± 0.524
2.097PheThr: 2.097 ± 0.328
2.485PheVal: 2.485 ± 0.484
0.854PheTrp: 0.854 ± 0.228
1.01PheTyr: 1.01 ± 0.29
0.0PheXaa: 0.0 ± 0.0
Gly
5.669GlyAla: 5.669 ± 0.901
0.854GlyCys: 0.854 ± 0.274
4.892GlyAsp: 4.892 ± 0.508
5.125GlyGlu: 5.125 ± 0.603
3.106GlyPhe: 3.106 ± 0.503
4.504GlyGly: 4.504 ± 0.477
0.932GlyHis: 0.932 ± 0.243
3.883GlyIle: 3.883 ± 0.521
4.504GlyLys: 4.504 ± 0.581
4.271GlyLeu: 4.271 ± 0.506
1.243GlyMet: 1.243 ± 0.317
3.417GlyAsn: 3.417 ± 0.544
1.553GlyPro: 1.553 ± 0.373
3.262GlyGln: 3.262 ± 0.527
3.495GlyArg: 3.495 ± 0.56
4.737GlySer: 4.737 ± 0.705
3.495GlyThr: 3.495 ± 0.57
4.659GlyVal: 4.659 ± 0.615
1.165GlyTrp: 1.165 ± 0.243
2.718GlyTyr: 2.718 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
0.854HisAla: 0.854 ± 0.29
0.466HisCys: 0.466 ± 0.237
0.388HisAsp: 0.388 ± 0.171
0.699HisGlu: 0.699 ± 0.22
1.165HisPhe: 1.165 ± 0.313
1.398HisGly: 1.398 ± 0.328
0.466HisHis: 0.466 ± 0.196
1.32HisIle: 1.32 ± 0.312
1.01HisLys: 1.01 ± 0.292
0.854HisLeu: 0.854 ± 0.226
0.466HisMet: 0.466 ± 0.164
0.544HisAsn: 0.544 ± 0.18
0.699HisPro: 0.699 ± 0.242
0.466HisGln: 0.466 ± 0.194
0.777HisArg: 0.777 ± 0.259
0.932HisSer: 0.932 ± 0.285
0.777HisThr: 0.777 ± 0.235
1.243HisVal: 1.243 ± 0.288
0.311HisTrp: 0.311 ± 0.174
0.388HisTyr: 0.388 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
5.98IleAla: 5.98 ± 0.619
0.699IleCys: 0.699 ± 0.171
4.97IleAsp: 4.97 ± 0.637
5.824IleGlu: 5.824 ± 0.722
1.243IlePhe: 1.243 ± 0.241
3.883IleGly: 3.883 ± 0.517
1.165IleHis: 1.165 ± 0.273
3.029IleIle: 3.029 ± 0.563
4.194IleLys: 4.194 ± 0.586
4.116IleLeu: 4.116 ± 0.638
1.32IleMet: 1.32 ± 0.379
3.805IleAsn: 3.805 ± 0.451
2.951IlePro: 2.951 ± 0.519
2.873IleGln: 2.873 ± 0.385
3.805IleArg: 3.805 ± 0.596
4.892IleSer: 4.892 ± 0.693
3.572IleThr: 3.572 ± 0.491
2.873IleVal: 2.873 ± 0.518
0.466IleTrp: 0.466 ± 0.162
2.097IleTyr: 2.097 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
5.98LysAla: 5.98 ± 0.757
0.466LysCys: 0.466 ± 0.183
4.038LysAsp: 4.038 ± 0.622
3.805LysGlu: 3.805 ± 0.552
2.64LysPhe: 2.64 ± 0.47
4.582LysGly: 4.582 ± 0.739
1.864LysHis: 1.864 ± 0.371
4.426LysIle: 4.426 ± 0.596
5.281LysLys: 5.281 ± 0.775
5.902LysLeu: 5.902 ± 0.633
2.019LysMet: 2.019 ± 0.421
3.339LysAsn: 3.339 ± 0.441
4.116LysPro: 4.116 ± 0.571
4.194LysGln: 4.194 ± 0.618
4.194LysArg: 4.194 ± 0.486
4.426LysSer: 4.426 ± 0.66
4.194LysThr: 4.194 ± 0.532
5.048LysVal: 5.048 ± 0.588
0.777LysTrp: 0.777 ± 0.261
2.407LysTyr: 2.407 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
8.076LeuAla: 8.076 ± 0.757
1.087LeuCys: 1.087 ± 0.28
5.591LeuAsp: 5.591 ± 0.605
4.97LeuGlu: 4.97 ± 0.549
2.951LeuPhe: 2.951 ± 0.443
5.824LeuGly: 5.824 ± 0.903
0.932LeuHis: 0.932 ± 0.293
5.358LeuIle: 5.358 ± 0.617
6.523LeuLys: 6.523 ± 0.644
6.29LeuLeu: 6.29 ± 0.665
1.941LeuMet: 1.941 ± 0.334
4.116LeuAsn: 4.116 ± 0.473
4.349LeuPro: 4.349 ± 0.658
3.262LeuGln: 3.262 ± 0.447
3.805LeuArg: 3.805 ± 0.52
4.892LeuSer: 4.892 ± 0.855
4.97LeuThr: 4.97 ± 0.588
4.815LeuVal: 4.815 ± 0.649
0.854LeuTrp: 0.854 ± 0.219
1.786LeuTyr: 1.786 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
2.873MetAla: 2.873 ± 0.723
0.311MetCys: 0.311 ± 0.123
1.087MetAsp: 1.087 ± 0.294
0.932MetGlu: 0.932 ± 0.287
1.087MetPhe: 1.087 ± 0.244
2.097MetGly: 2.097 ± 0.455
0.078MetHis: 0.078 ± 0.075
1.786MetIle: 1.786 ± 0.298
1.941MetLys: 1.941 ± 0.394
2.796MetLeu: 2.796 ± 0.439
0.854MetMet: 0.854 ± 0.271
1.087MetAsn: 1.087 ± 0.292
1.475MetPro: 1.475 ± 0.376
0.466MetGln: 0.466 ± 0.249
1.243MetArg: 1.243 ± 0.25
1.475MetSer: 1.475 ± 0.324
1.475MetThr: 1.475 ± 0.279
1.864MetVal: 1.864 ± 0.324
0.155MetTrp: 0.155 ± 0.155
0.544MetTyr: 0.544 ± 0.229
0.0MetXaa: 0.0 ± 0.0
Asn
3.029AsnAla: 3.029 ± 0.504
0.388AsnCys: 0.388 ± 0.14
1.475AsnAsp: 1.475 ± 0.309
2.33AsnGlu: 2.33 ± 0.378
2.33AsnPhe: 2.33 ± 0.475
4.504AsnGly: 4.504 ± 0.616
1.087AsnHis: 1.087 ± 0.323
2.33AsnIle: 2.33 ± 0.326
3.029AsnLys: 3.029 ± 0.469
3.961AsnLeu: 3.961 ± 0.554
1.32AsnMet: 1.32 ± 0.249
2.64AsnAsn: 2.64 ± 0.57
2.252AsnPro: 2.252 ± 0.45
2.485AsnGln: 2.485 ± 0.443
2.563AsnArg: 2.563 ± 0.447
2.019AsnSer: 2.019 ± 0.349
2.951AsnThr: 2.951 ± 0.405
2.563AsnVal: 2.563 ± 0.447
0.854AsnTrp: 0.854 ± 0.275
1.165AsnTyr: 1.165 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
2.64ProAla: 2.64 ± 0.519
0.233ProCys: 0.233 ± 0.152
3.029ProAsp: 3.029 ± 0.458
3.728ProGlu: 3.728 ± 0.643
1.32ProPhe: 1.32 ± 0.302
2.097ProGly: 2.097 ± 0.364
0.311ProHis: 0.311 ± 0.143
2.718ProIle: 2.718 ± 0.41
3.572ProLys: 3.572 ± 0.579
2.563ProLeu: 2.563 ± 0.397
1.398ProMet: 1.398 ± 0.315
1.708ProAsn: 1.708 ± 0.344
1.32ProPro: 1.32 ± 0.324
1.398ProGln: 1.398 ± 0.407
1.631ProArg: 1.631 ± 0.322
3.572ProSer: 3.572 ± 0.595
2.873ProThr: 2.873 ± 0.465
2.951ProVal: 2.951 ± 0.553
0.621ProTrp: 0.621 ± 0.271
1.32ProTyr: 1.32 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
3.883GlnAla: 3.883 ± 0.727
0.311GlnCys: 0.311 ± 0.141
1.941GlnAsp: 1.941 ± 0.39
2.873GlnGlu: 2.873 ± 0.42
1.165GlnPhe: 1.165 ± 0.255
1.786GlnGly: 1.786 ± 0.351
0.777GlnHis: 0.777 ± 0.22
3.339GlnIle: 3.339 ± 0.593
3.572GlnLys: 3.572 ± 0.557
4.038GlnLeu: 4.038 ± 0.539
1.243GlnMet: 1.243 ± 0.258
1.708GlnAsn: 1.708 ± 0.33
1.631GlnPro: 1.631 ± 0.356
2.485GlnGln: 2.485 ± 0.434
2.796GlnArg: 2.796 ± 0.366
3.339GlnSer: 3.339 ± 0.527
2.097GlnThr: 2.097 ± 0.39
2.563GlnVal: 2.563 ± 0.405
0.932GlnTrp: 0.932 ± 0.237
0.854GlnTyr: 0.854 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
4.271ArgAla: 4.271 ± 0.704
0.388ArgCys: 0.388 ± 0.172
2.718ArgAsp: 2.718 ± 0.477
4.038ArgGlu: 4.038 ± 0.568
1.708ArgPhe: 1.708 ± 0.316
2.407ArgGly: 2.407 ± 0.392
0.544ArgHis: 0.544 ± 0.161
3.184ArgIle: 3.184 ± 0.533
2.951ArgLys: 2.951 ± 0.514
5.514ArgLeu: 5.514 ± 0.733
1.631ArgMet: 1.631 ± 0.439
1.941ArgAsn: 1.941 ± 0.44
1.786ArgPro: 1.786 ± 0.39
1.941ArgGln: 1.941 ± 0.375
2.407ArgArg: 2.407 ± 0.511
2.718ArgSer: 2.718 ± 0.38
2.951ArgThr: 2.951 ± 0.452
3.728ArgVal: 3.728 ± 0.447
0.388ArgTrp: 0.388 ± 0.162
2.252ArgTyr: 2.252 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
4.892SerAla: 4.892 ± 0.677
0.544SerCys: 0.544 ± 0.168
4.582SerAsp: 4.582 ± 0.77
5.203SerGlu: 5.203 ± 0.64
1.941SerPhe: 1.941 ± 0.392
5.125SerGly: 5.125 ± 0.621
1.631SerHis: 1.631 ± 0.361
5.048SerIle: 5.048 ± 0.641
5.902SerLys: 5.902 ± 0.509
5.203SerLeu: 5.203 ± 0.62
1.553SerMet: 1.553 ± 0.358
2.951SerAsn: 2.951 ± 0.515
2.097SerPro: 2.097 ± 0.373
2.563SerGln: 2.563 ± 0.486
2.873SerArg: 2.873 ± 0.546
4.815SerSer: 4.815 ± 0.83
3.029SerThr: 3.029 ± 0.439
4.426SerVal: 4.426 ± 0.482
0.854SerTrp: 0.854 ± 0.234
1.864SerTyr: 1.864 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
4.349ThrAla: 4.349 ± 0.803
0.466ThrCys: 0.466 ± 0.198
3.417ThrAsp: 3.417 ± 0.605
3.805ThrGlu: 3.805 ± 0.564
2.485ThrPhe: 2.485 ± 0.408
4.815ThrGly: 4.815 ± 0.616
0.932ThrHis: 0.932 ± 0.34
3.805ThrIle: 3.805 ± 0.499
4.116ThrLys: 4.116 ± 0.594
3.961ThrLeu: 3.961 ± 0.514
1.01ThrMet: 1.01 ± 0.343
1.786ThrAsn: 1.786 ± 0.377
2.873ThrPro: 2.873 ± 0.33
2.097ThrGln: 2.097 ± 0.313
1.864ThrArg: 1.864 ± 0.452
2.718ThrSer: 2.718 ± 0.537
2.563ThrThr: 2.563 ± 0.443
4.194ThrVal: 4.194 ± 0.728
0.621ThrTrp: 0.621 ± 0.171
1.553ThrTyr: 1.553 ± 0.38
0.0ThrXaa: 0.0 ± 0.0
Val
4.815ValAla: 4.815 ± 0.615
0.466ValCys: 0.466 ± 0.179
4.582ValAsp: 4.582 ± 0.702
3.961ValGlu: 3.961 ± 0.613
1.864ValPhe: 1.864 ± 0.351
3.961ValGly: 3.961 ± 0.457
0.699ValHis: 0.699 ± 0.256
4.815ValIle: 4.815 ± 0.612
5.591ValLys: 5.591 ± 0.512
5.048ValLeu: 5.048 ± 0.784
1.243ValMet: 1.243 ± 0.272
3.495ValAsn: 3.495 ± 0.519
2.951ValPro: 2.951 ± 0.463
2.563ValGln: 2.563 ± 0.466
3.262ValArg: 3.262 ± 0.393
4.659ValSer: 4.659 ± 0.528
4.038ValThr: 4.038 ± 0.6
4.582ValVal: 4.582 ± 0.575
0.777ValTrp: 0.777 ± 0.219
2.019ValTyr: 2.019 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
0.777TrpAla: 0.777 ± 0.221
0.155TrpCys: 0.155 ± 0.107
1.165TrpAsp: 1.165 ± 0.302
0.932TrpGlu: 0.932 ± 0.233
0.854TrpPhe: 0.854 ± 0.285
1.398TrpGly: 1.398 ± 0.371
0.311TrpHis: 0.311 ± 0.142
1.087TrpIle: 1.087 ± 0.278
1.087TrpLys: 1.087 ± 0.317
1.786TrpLeu: 1.786 ± 0.386
0.388TrpMet: 0.388 ± 0.152
0.233TrpAsn: 0.233 ± 0.127
0.233TrpPro: 0.233 ± 0.115
0.699TrpGln: 0.699 ± 0.246
1.01TrpArg: 1.01 ± 0.291
1.01TrpSer: 1.01 ± 0.219
0.544TrpThr: 0.544 ± 0.253
1.01TrpVal: 1.01 ± 0.289
0.155TrpTrp: 0.155 ± 0.098
0.388TrpTyr: 0.388 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.475TyrAla: 1.475 ± 0.36
0.621TyrCys: 0.621 ± 0.222
1.708TyrAsp: 1.708 ± 0.35
2.951TyrGlu: 2.951 ± 0.469
1.243TyrPhe: 1.243 ± 0.266
1.475TyrGly: 1.475 ± 0.297
0.621TyrHis: 0.621 ± 0.238
1.708TyrIle: 1.708 ± 0.366
2.174TyrLys: 2.174 ± 0.342
2.796TyrLeu: 2.796 ± 0.434
0.233TyrMet: 0.233 ± 0.139
1.087TyrAsn: 1.087 ± 0.242
0.932TyrPro: 0.932 ± 0.306
1.243TyrGln: 1.243 ± 0.32
1.941TyrArg: 1.941 ± 0.357
1.864TyrSer: 1.864 ± 0.351
2.174TyrThr: 2.174 ± 0.419
1.864TyrVal: 1.864 ± 0.39
0.854TyrTrp: 0.854 ± 0.229
1.01TyrTyr: 1.01 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski