Candidatus Berkiella cookevillensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Candidatus Berkiella

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9YMV1|A0A0Q9YMV1_9COXI DDE_Tnp_ISL3 domain-containing protein OS=Candidatus Berkiella cookevillensis OX=437022 GN=CC99x_02439 PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84HH3 pKa = 4.49SHH5 pKa = 5.27QPYY8 pKa = 7.66VGKK11 pKa = 9.58WYY13 pKa = 10.37RR14 pKa = 11.84NHH16 pKa = 7.63DD17 pKa = 3.53INNIFEE23 pKa = 4.49VVASDD28 pKa = 3.98DD29 pKa = 3.94DD30 pKa = 4.18SVEE33 pKa = 3.69IQYY36 pKa = 10.39FAGEE40 pKa = 4.16IEE42 pKa = 4.53EE43 pKa = 5.21LDD45 pKa = 3.64LDD47 pKa = 3.6TWYY50 pKa = 10.47DD51 pKa = 3.67LDD53 pKa = 4.85LKK55 pKa = 10.62QIPAPEE61 pKa = 4.42DD62 pKa = 2.9WSGPFEE68 pKa = 5.17LSHH71 pKa = 8.79DD72 pKa = 3.8DD73 pKa = 3.28MGYY76 pKa = 10.36SDD78 pKa = 4.54EE79 pKa = 4.43TLHH82 pKa = 6.78PEE84 pKa = 4.18DD85 pKa = 3.29WSGPLAEE92 pKa = 5.54LEE94 pKa = 4.59PDD96 pKa = 3.71DD97 pKa = 6.43DD98 pKa = 4.35YY99 pKa = 12.31

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9YC95|A0A0Q9YC95_9COXI Uncharacterized protein OS=Candidatus Berkiella cookevillensis OX=437022 GN=CC99x_01697 PE=4 SV=1
MM1 pKa = 7.72AARR4 pKa = 11.84KK5 pKa = 8.08KK6 pKa = 8.6TAAKK10 pKa = 7.05TTGTTVKK17 pKa = 10.24KK18 pKa = 9.75ATRR21 pKa = 11.84KK22 pKa = 6.24TVKK25 pKa = 10.24RR26 pKa = 11.84KK27 pKa = 7.51TAAKK31 pKa = 9.55AAKK34 pKa = 9.67KK35 pKa = 8.79KK36 pKa = 8.65TAAKK40 pKa = 10.0AKK42 pKa = 10.11KK43 pKa = 9.53AVKK46 pKa = 10.09KK47 pKa = 9.35KK48 pKa = 8.41TAAKK52 pKa = 9.84RR53 pKa = 11.84KK54 pKa = 7.03TVAKK58 pKa = 10.14KK59 pKa = 7.99VTKK62 pKa = 10.24KK63 pKa = 6.72VTKK66 pKa = 10.45RR67 pKa = 11.84KK68 pKa = 6.45VTKK71 pKa = 10.76AKK73 pKa = 8.67VTKK76 pKa = 10.52AKK78 pKa = 10.46AATKK82 pKa = 10.3RR83 pKa = 11.84KK84 pKa = 7.19TAAKK88 pKa = 9.87KK89 pKa = 7.91PTAAKK94 pKa = 8.7TATKK98 pKa = 10.09KK99 pKa = 10.63RR100 pKa = 11.84KK101 pKa = 6.08TTAKK105 pKa = 10.0PKK107 pKa = 9.24AAKK110 pKa = 10.43AKK112 pKa = 8.06GTVVKK117 pKa = 10.34AVKK120 pKa = 9.52AAKK123 pKa = 10.01KK124 pKa = 10.11VVAKK128 pKa = 9.93VKK130 pKa = 9.33RR131 pKa = 11.84TVRR134 pKa = 11.84KK135 pKa = 9.19KK136 pKa = 10.65AKK138 pKa = 9.83KK139 pKa = 9.77AA140 pKa = 3.33

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2537

0

2537

872039

29

3906

343.7

38.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.935 ± 0.045

1.103 ± 0.019

5.021 ± 0.052

6.286 ± 0.051

4.394 ± 0.036

5.652 ± 0.054

2.517 ± 0.025

7.536 ± 0.04

6.854 ± 0.059

10.644 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.021

4.831 ± 0.046

3.906 ± 0.029

4.681 ± 0.038

4.067 ± 0.039

6.762 ± 0.038

5.123 ± 0.035

5.901 ± 0.044

1.071 ± 0.019

3.31 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski