Lactococcus phage PLg-TB25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9IGH5|A0A1X9IGH5_9CAUD Uncharacterized protein OS=Lactococcus phage PLg-TB25 OX=1913145 PE=4 SV=1
MM1 pKa = 7.91EE2 pKa = 5.46ILNPQAEE9 pKa = 4.55LTNALLHH16 pKa = 5.78VLPLLDD22 pKa = 3.39VDD24 pKa = 4.06TYY26 pKa = 11.25FYY28 pKa = 11.01LPQEE32 pKa = 4.01AVKK35 pKa = 11.04YY36 pKa = 8.04PFIVVGEE43 pKa = 3.98QLATGTYY50 pKa = 7.73TKK52 pKa = 10.28EE53 pKa = 3.69RR54 pKa = 11.84SIVSLKK60 pKa = 10.36QVIHH64 pKa = 6.27VYY66 pKa = 10.87ALADD70 pKa = 3.84EE71 pKa = 4.77LPYY74 pKa = 10.2IQRR77 pKa = 11.84LVKK80 pKa = 10.59LIEE83 pKa = 4.0ATVFNLQKK91 pKa = 9.78TEE93 pKa = 3.65NFNWVNTLDD102 pKa = 4.09EE103 pKa = 5.31DD104 pKa = 4.31VLLMDD109 pKa = 6.5DD110 pKa = 3.81STKK113 pKa = 11.12DD114 pKa = 3.88QLWHH118 pKa = 6.03AAITFSCNSNN128 pKa = 3.11

Molecular weight: 14.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9IGJ1|A0A1X9IGJ1_9CAUD Uncharacterized protein OS=Lactococcus phage PLg-TB25 OX=1913145 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.12RR3 pKa = 11.84NKK5 pKa = 9.36GRR7 pKa = 11.84EE8 pKa = 3.97WLGGPGLVILVIMAPFAVILFVQIITAIYY37 pKa = 9.86LYY39 pKa = 10.95LL40 pKa = 5.5

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11360

38

1358

195.9

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.735

0.493 ± 0.118

5.995 ± 0.226

8.037 ± 0.49

4.331 ± 0.179

5.889 ± 0.451

1.215 ± 0.167

7.148 ± 0.359

9.085 ± 0.412

8.644 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.155

5.96 ± 0.223

2.65 ± 0.223

3.873 ± 0.313

3.785 ± 0.218

7.104 ± 0.408

5.915 ± 0.297

5.546 ± 0.229

1.039 ± 0.137

4.032 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski