Amino acid dipepetide frequency for Lactococcus phage PLg-TB25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.018AlaAla: 5.018 ± 1.333
0.44AlaCys: 0.44 ± 0.31
4.314AlaAsp: 4.314 ± 0.649
4.666AlaGlu: 4.666 ± 0.632
2.817AlaPhe: 2.817 ± 0.597
4.05AlaGly: 4.05 ± 0.904
0.704AlaHis: 0.704 ± 0.22
4.138AlaIle: 4.138 ± 0.688
5.458AlaLys: 5.458 ± 0.816
5.986AlaLeu: 5.986 ± 0.742
2.113AlaMet: 2.113 ± 0.481
3.962AlaAsn: 3.962 ± 0.6
1.321AlaPro: 1.321 ± 0.356
2.905AlaGln: 2.905 ± 0.708
2.729AlaArg: 2.729 ± 0.587
4.138AlaSer: 4.138 ± 0.938
5.194AlaThr: 5.194 ± 0.943
3.609AlaVal: 3.609 ± 0.833
0.44AlaTrp: 0.44 ± 0.187
2.377AlaTyr: 2.377 ± 0.33
0.0AlaXaa: 0.0 ± 0.0
Cys
0.264CysAla: 0.264 ± 0.147
0.0CysCys: 0.0 ± 0.0
0.616CysAsp: 0.616 ± 0.311
0.352CysGlu: 0.352 ± 0.197
0.088CysPhe: 0.088 ± 0.076
0.264CysGly: 0.264 ± 0.145
0.264CysHis: 0.264 ± 0.133
0.352CysIle: 0.352 ± 0.18
0.264CysLys: 0.264 ± 0.145
0.704CysLeu: 0.704 ± 0.318
0.088CysMet: 0.088 ± 0.08
0.352CysAsn: 0.352 ± 0.206
0.176CysPro: 0.176 ± 0.104
0.176CysGln: 0.176 ± 0.11
0.176CysArg: 0.176 ± 0.115
0.264CysSer: 0.264 ± 0.147
0.176CysThr: 0.176 ± 0.115
0.264CysVal: 0.264 ± 0.131
0.0CysTrp: 0.0 ± 0.0
0.088CysTyr: 0.088 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
3.257AspAla: 3.257 ± 0.625
0.0AspCys: 0.0 ± 0.0
3.169AspAsp: 3.169 ± 0.515
4.754AspGlu: 4.754 ± 0.961
3.257AspPhe: 3.257 ± 0.608
3.257AspGly: 3.257 ± 0.507
0.44AspHis: 0.44 ± 0.153
4.754AspIle: 4.754 ± 0.659
5.282AspLys: 5.282 ± 0.539
5.37AspLeu: 5.37 ± 0.679
1.497AspMet: 1.497 ± 0.382
4.842AspAsn: 4.842 ± 0.553
1.497AspPro: 1.497 ± 0.31
1.056AspGln: 1.056 ± 0.317
2.377AspArg: 2.377 ± 0.48
4.05AspSer: 4.05 ± 0.712
3.874AspThr: 3.874 ± 0.601
2.905AspVal: 2.905 ± 0.485
0.704AspTrp: 0.704 ± 0.237
3.609AspTyr: 3.609 ± 0.71
0.0AspXaa: 0.0 ± 0.0
Glu
5.194GluAla: 5.194 ± 0.736
0.528GluCys: 0.528 ± 0.232
3.698GluAsp: 3.698 ± 0.49
7.659GluGlu: 7.659 ± 1.118
3.169GluPhe: 3.169 ± 0.581
3.257GluGly: 3.257 ± 0.404
1.144GluHis: 1.144 ± 0.426
5.282GluIle: 5.282 ± 0.618
8.628GluLys: 8.628 ± 0.905
9.068GluLeu: 9.068 ± 1.15
1.497GluMet: 1.497 ± 0.318
5.81GluAsn: 5.81 ± 0.709
1.673GluPro: 1.673 ± 0.553
3.081GluGln: 3.081 ± 0.668
2.817GluArg: 2.817 ± 0.461
4.226GluSer: 4.226 ± 0.495
4.05GluThr: 4.05 ± 0.821
4.754GluVal: 4.754 ± 0.64
0.88GluTrp: 0.88 ± 0.3
3.962GluTyr: 3.962 ± 0.667
0.0GluXaa: 0.0 ± 0.0
Phe
2.201PheAla: 2.201 ± 0.405
0.352PheCys: 0.352 ± 0.177
3.345PheAsp: 3.345 ± 0.436
3.521PheGlu: 3.521 ± 0.523
1.849PhePhe: 1.849 ± 0.372
3.257PheGly: 3.257 ± 0.471
0.528PheHis: 0.528 ± 0.215
3.609PheIle: 3.609 ± 0.553
3.521PheLys: 3.521 ± 0.459
2.465PheLeu: 2.465 ± 0.541
1.673PheMet: 1.673 ± 0.312
2.201PheAsn: 2.201 ± 0.402
1.673PhePro: 1.673 ± 0.297
1.673PheGln: 1.673 ± 0.366
1.144PheArg: 1.144 ± 0.25
3.874PheSer: 3.874 ± 0.586
2.641PheThr: 2.641 ± 0.438
1.673PheVal: 1.673 ± 0.374
0.44PheTrp: 0.44 ± 0.19
1.673PheTyr: 1.673 ± 0.358
0.0PheXaa: 0.0 ± 0.0
Gly
4.666GlyAla: 4.666 ± 0.863
0.264GlyCys: 0.264 ± 0.155
2.905GlyAsp: 2.905 ± 0.731
4.402GlyGlu: 4.402 ± 0.692
3.433GlyPhe: 3.433 ± 0.65
3.521GlyGly: 3.521 ± 0.666
0.528GlyHis: 0.528 ± 0.187
5.018GlyIle: 5.018 ± 0.729
5.722GlyLys: 5.722 ± 0.681
4.314GlyLeu: 4.314 ± 0.536
1.673GlyMet: 1.673 ± 0.374
3.433GlyAsn: 3.433 ± 0.53
0.352GlyPro: 0.352 ± 0.173
1.585GlyGln: 1.585 ± 0.475
2.113GlyArg: 2.113 ± 0.316
4.578GlySer: 4.578 ± 0.864
3.786GlyThr: 3.786 ± 0.856
3.874GlyVal: 3.874 ± 0.666
0.44GlyTrp: 0.44 ± 0.191
2.289GlyTyr: 2.289 ± 0.602
0.0GlyXaa: 0.0 ± 0.0
His
0.352HisAla: 0.352 ± 0.176
0.088HisCys: 0.088 ± 0.1
0.616HisAsp: 0.616 ± 0.293
1.056HisGlu: 1.056 ± 0.35
0.88HisPhe: 0.88 ± 0.26
0.792HisGly: 0.792 ± 0.298
0.088HisHis: 0.088 ± 0.089
0.88HisIle: 0.88 ± 0.353
0.88HisLys: 0.88 ± 0.329
0.968HisLeu: 0.968 ± 0.299
0.176HisMet: 0.176 ± 0.125
0.616HisAsn: 0.616 ± 0.264
0.616HisPro: 0.616 ± 0.244
0.264HisGln: 0.264 ± 0.152
0.176HisArg: 0.176 ± 0.122
1.321HisSer: 1.321 ± 0.339
0.616HisThr: 0.616 ± 0.247
1.144HisVal: 1.144 ± 0.363
0.176HisTrp: 0.176 ± 0.126
0.44HisTyr: 0.44 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.666IleAla: 4.666 ± 0.804
0.352IleCys: 0.352 ± 0.161
4.93IleAsp: 4.93 ± 0.713
6.251IleGlu: 6.251 ± 0.886
2.553IlePhe: 2.553 ± 0.493
3.345IleGly: 3.345 ± 0.515
0.792IleHis: 0.792 ± 0.39
5.282IleIle: 5.282 ± 0.785
7.043IleLys: 7.043 ± 0.8
5.194IleLeu: 5.194 ± 0.621
1.144IleMet: 1.144 ± 0.304
3.962IleAsn: 3.962 ± 0.518
2.553IlePro: 2.553 ± 0.397
3.257IleGln: 3.257 ± 0.532
2.289IleArg: 2.289 ± 0.429
5.634IleSer: 5.634 ± 0.721
4.402IleThr: 4.402 ± 0.568
4.138IleVal: 4.138 ± 0.598
0.704IleTrp: 0.704 ± 0.264
2.993IleTyr: 2.993 ± 0.577
0.0IleXaa: 0.0 ± 0.0
Lys
5.898LysAla: 5.898 ± 0.795
0.44LysCys: 0.44 ± 0.21
5.722LysAsp: 5.722 ± 0.695
8.98LysGlu: 8.98 ± 1.174
3.433LysPhe: 3.433 ± 0.473
5.458LysGly: 5.458 ± 0.796
1.233LysHis: 1.233 ± 0.313
5.81LysIle: 5.81 ± 0.665
7.835LysLys: 7.835 ± 1.14
7.395LysLeu: 7.395 ± 0.869
3.345LysMet: 3.345 ± 0.467
4.93LysAsn: 4.93 ± 0.767
1.673LysPro: 1.673 ± 0.336
4.49LysGln: 4.49 ± 0.749
4.05LysArg: 4.05 ± 0.591
6.163LysSer: 6.163 ± 0.638
5.458LysThr: 5.458 ± 0.649
4.402LysVal: 4.402 ± 0.577
0.616LysTrp: 0.616 ± 0.237
3.521LysTyr: 3.521 ± 0.609
0.0LysXaa: 0.0 ± 0.0
Leu
5.546LeuAla: 5.546 ± 0.79
0.704LeuCys: 0.704 ± 0.267
5.986LeuAsp: 5.986 ± 0.73
7.395LeuGlu: 7.395 ± 1.089
3.521LeuPhe: 3.521 ± 0.641
6.074LeuGly: 6.074 ± 0.946
0.704LeuHis: 0.704 ± 0.223
6.251LeuIle: 6.251 ± 0.534
8.363LeuLys: 8.363 ± 0.865
7.043LeuLeu: 7.043 ± 0.907
2.377LeuMet: 2.377 ± 0.463
4.49LeuAsn: 4.49 ± 0.657
2.113LeuPro: 2.113 ± 0.424
2.905LeuGln: 2.905 ± 0.454
2.377LeuArg: 2.377 ± 0.471
7.043LeuSer: 7.043 ± 0.747
4.93LeuThr: 4.93 ± 0.646
5.194LeuVal: 5.194 ± 0.678
1.056LeuTrp: 1.056 ± 0.323
2.377LeuTyr: 2.377 ± 0.618
0.0LeuXaa: 0.0 ± 0.0
Met
2.289MetAla: 2.289 ± 0.415
0.0MetCys: 0.0 ± 0.0
1.673MetAsp: 1.673 ± 0.329
2.289MetGlu: 2.289 ± 0.451
0.968MetPhe: 0.968 ± 0.308
0.616MetGly: 0.616 ± 0.203
0.44MetHis: 0.44 ± 0.214
1.761MetIle: 1.761 ± 0.48
2.465MetLys: 2.465 ± 0.446
1.585MetLeu: 1.585 ± 0.334
0.528MetMet: 0.528 ± 0.251
1.761MetAsn: 1.761 ± 0.392
0.792MetPro: 0.792 ± 0.287
0.792MetGln: 0.792 ± 0.551
1.497MetArg: 1.497 ± 0.369
2.289MetSer: 2.289 ± 0.523
1.937MetThr: 1.937 ± 0.437
1.321MetVal: 1.321 ± 0.337
0.352MetTrp: 0.352 ± 0.167
0.88MetTyr: 0.88 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.874AsnAla: 3.874 ± 0.986
0.352AsnCys: 0.352 ± 0.146
3.081AsnAsp: 3.081 ± 0.484
3.874AsnGlu: 3.874 ± 0.598
3.081AsnPhe: 3.081 ± 0.614
4.578AsnGly: 4.578 ± 0.568
0.528AsnHis: 0.528 ± 0.2
3.874AsnIle: 3.874 ± 0.831
5.458AsnLys: 5.458 ± 0.765
4.842AsnLeu: 4.842 ± 0.595
1.849AsnMet: 1.849 ± 0.343
3.169AsnAsn: 3.169 ± 0.566
2.289AsnPro: 2.289 ± 0.473
2.465AsnGln: 2.465 ± 0.478
2.553AsnArg: 2.553 ± 0.473
3.521AsnSer: 3.521 ± 0.779
3.345AsnThr: 3.345 ± 0.444
3.169AsnVal: 3.169 ± 0.566
0.968AsnTrp: 0.968 ± 0.25
2.729AsnTyr: 2.729 ± 0.449
0.0AsnXaa: 0.0 ± 0.0
Pro
1.233ProAla: 1.233 ± 0.352
0.088ProCys: 0.088 ± 0.08
1.761ProAsp: 1.761 ± 0.331
2.465ProGlu: 2.465 ± 0.576
1.761ProPhe: 1.761 ± 0.41
0.968ProGly: 0.968 ± 0.295
0.704ProHis: 0.704 ± 0.266
1.585ProIle: 1.585 ± 0.394
2.113ProLys: 2.113 ± 0.473
2.289ProLeu: 2.289 ± 0.501
0.704ProMet: 0.704 ± 0.244
1.056ProAsn: 1.056 ± 0.5
0.44ProPro: 0.44 ± 0.176
1.585ProGln: 1.585 ± 0.35
0.704ProArg: 0.704 ± 0.305
2.993ProSer: 2.993 ± 0.57
1.673ProThr: 1.673 ± 0.524
1.321ProVal: 1.321 ± 0.296
0.088ProTrp: 0.088 ± 0.1
0.968ProTyr: 0.968 ± 0.27
0.0ProXaa: 0.0 ± 0.0
Gln
2.993GlnAla: 2.993 ± 0.607
0.088GlnCys: 0.088 ± 0.078
2.377GlnAsp: 2.377 ± 0.421
2.817GlnGlu: 2.817 ± 0.596
1.321GlnPhe: 1.321 ± 0.329
2.201GlnGly: 2.201 ± 0.529
0.528GlnHis: 0.528 ± 0.216
2.905GlnIle: 2.905 ± 0.477
3.521GlnLys: 3.521 ± 0.58
3.433GlnLeu: 3.433 ± 0.515
0.88GlnMet: 0.88 ± 0.369
2.905GlnAsn: 2.905 ± 0.641
1.321GlnPro: 1.321 ± 0.538
1.761GlnGln: 1.761 ± 0.476
2.465GlnArg: 2.465 ± 0.458
2.113GlnSer: 2.113 ± 0.614
2.025GlnThr: 2.025 ± 0.349
1.585GlnVal: 1.585 ± 0.34
0.352GlnTrp: 0.352 ± 0.161
1.144GlnTyr: 1.144 ± 0.327
0.0GlnXaa: 0.0 ± 0.0
Arg
1.937ArgAla: 1.937 ± 0.384
0.352ArgCys: 0.352 ± 0.171
1.937ArgAsp: 1.937 ± 0.299
3.169ArgGlu: 3.169 ± 0.517
1.144ArgPhe: 1.144 ± 0.32
1.761ArgGly: 1.761 ± 0.392
0.616ArgHis: 0.616 ± 0.282
2.553ArgIle: 2.553 ± 0.496
2.817ArgLys: 2.817 ± 0.651
4.666ArgLeu: 4.666 ± 0.696
1.497ArgMet: 1.497 ± 0.392
2.113ArgAsn: 2.113 ± 0.395
1.144ArgPro: 1.144 ± 0.35
1.849ArgGln: 1.849 ± 0.427
1.497ArgArg: 1.497 ± 0.366
2.201ArgSer: 2.201 ± 0.525
1.585ArgThr: 1.585 ± 0.428
2.289ArgVal: 2.289 ± 0.483
0.528ArgTrp: 0.528 ± 0.21
2.201ArgTyr: 2.201 ± 0.464
0.0ArgXaa: 0.0 ± 0.0
Ser
5.282SerAla: 5.282 ± 1.309
0.176SerCys: 0.176 ± 0.133
4.05SerAsp: 4.05 ± 0.532
4.666SerGlu: 4.666 ± 0.692
3.609SerPhe: 3.609 ± 0.686
5.106SerGly: 5.106 ± 0.788
0.792SerHis: 0.792 ± 0.319
5.194SerIle: 5.194 ± 0.623
6.427SerLys: 6.427 ± 0.496
5.634SerLeu: 5.634 ± 0.911
1.937SerMet: 1.937 ± 0.386
5.194SerAsn: 5.194 ± 0.666
1.497SerPro: 1.497 ± 0.337
2.993SerGln: 2.993 ± 0.506
2.465SerArg: 2.465 ± 0.368
5.106SerSer: 5.106 ± 0.599
3.081SerThr: 3.081 ± 0.483
3.874SerVal: 3.874 ± 0.631
0.616SerTrp: 0.616 ± 0.287
3.257SerTyr: 3.257 ± 0.595
0.0SerXaa: 0.0 ± 0.0
Thr
5.018ThrAla: 5.018 ± 1.226
0.088ThrCys: 0.088 ± 0.088
3.081ThrAsp: 3.081 ± 0.463
4.226ThrGlu: 4.226 ± 0.567
2.377ThrPhe: 2.377 ± 0.501
3.874ThrGly: 3.874 ± 0.556
1.144ThrHis: 1.144 ± 0.333
4.402ThrIle: 4.402 ± 0.725
5.634ThrLys: 5.634 ± 0.668
5.81ThrLeu: 5.81 ± 0.82
0.528ThrMet: 0.528 ± 0.199
2.729ThrAsn: 2.729 ± 0.683
2.729ThrPro: 2.729 ± 0.798
1.497ThrGln: 1.497 ± 0.291
2.729ThrArg: 2.729 ± 0.49
3.081ThrSer: 3.081 ± 0.657
2.905ThrThr: 2.905 ± 0.583
4.226ThrVal: 4.226 ± 0.627
0.792ThrTrp: 0.792 ± 0.205
2.289ThrTyr: 2.289 ± 0.602
0.0ThrXaa: 0.0 ± 0.0
Val
3.874ValAla: 3.874 ± 1.088
0.0ValCys: 0.0 ± 0.0
3.698ValAsp: 3.698 ± 0.534
3.962ValGlu: 3.962 ± 0.554
2.465ValPhe: 2.465 ± 0.477
3.081ValGly: 3.081 ± 0.569
0.528ValHis: 0.528 ± 0.261
4.138ValIle: 4.138 ± 0.82
5.458ValLys: 5.458 ± 0.716
4.578ValLeu: 4.578 ± 0.447
1.585ValMet: 1.585 ± 0.392
3.433ValAsn: 3.433 ± 0.457
1.673ValPro: 1.673 ± 0.326
2.201ValGln: 2.201 ± 0.479
1.144ValArg: 1.144 ± 0.333
4.49ValSer: 4.49 ± 0.575
4.226ValThr: 4.226 ± 0.539
2.993ValVal: 2.993 ± 0.537
0.704ValTrp: 0.704 ± 0.24
1.233ValTyr: 1.233 ± 0.275
0.0ValXaa: 0.0 ± 0.0
Trp
0.528TrpAla: 0.528 ± 0.227
0.352TrpCys: 0.352 ± 0.147
0.704TrpAsp: 0.704 ± 0.286
0.792TrpGlu: 0.792 ± 0.343
0.352TrpPhe: 0.352 ± 0.17
0.792TrpGly: 0.792 ± 0.24
0.176TrpHis: 0.176 ± 0.126
0.352TrpIle: 0.352 ± 0.154
0.792TrpLys: 0.792 ± 0.26
0.88TrpLeu: 0.88 ± 0.218
0.0TrpMet: 0.0 ± 0.0
0.528TrpAsn: 0.528 ± 0.273
0.176TrpPro: 0.176 ± 0.123
0.44TrpGln: 0.44 ± 0.197
0.176TrpArg: 0.176 ± 0.109
0.968TrpSer: 0.968 ± 0.287
0.792TrpThr: 0.792 ± 0.236
0.704TrpVal: 0.704 ± 0.247
0.0TrpTrp: 0.0 ± 0.0
0.88TrpTyr: 0.88 ± 0.342
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.289TyrAla: 2.289 ± 0.465
0.264TyrCys: 0.264 ± 0.149
2.289TyrAsp: 2.289 ± 0.547
3.081TyrGlu: 3.081 ± 0.58
1.233TyrPhe: 1.233 ± 0.387
2.641TyrGly: 2.641 ± 0.534
0.264TyrHis: 0.264 ± 0.148
3.345TyrIle: 3.345 ± 0.67
3.169TyrLys: 3.169 ± 0.477
4.226TyrLeu: 4.226 ± 0.631
1.056TyrMet: 1.056 ± 0.344
2.113TyrAsn: 2.113 ± 0.513
0.792TyrPro: 0.792 ± 0.28
1.761TyrGln: 1.761 ± 0.393
2.377TyrArg: 2.377 ± 0.441
2.993TyrSer: 2.993 ± 0.488
2.465TyrThr: 2.465 ± 0.541
2.025TyrVal: 2.025 ± 0.408
0.528TyrTrp: 0.528 ± 0.235
1.409TyrTyr: 1.409 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11360 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski