Romboutsia maritimum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Romboutsia

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371IUP8|A0A371IUP8_9FIRM AraC family transcriptional regulator OS=Romboutsia maritimum OX=2020948 GN=CHF27_003755 PE=4 SV=1
MM1 pKa = 7.11SQYY4 pKa = 11.12ILLTSDD10 pKa = 4.05CEE12 pKa = 4.18LPEE15 pKa = 5.71IDD17 pKa = 5.03LTNKK21 pKa = 9.91QNITVEE27 pKa = 3.9EE28 pKa = 4.08AKK30 pKa = 10.45IKK32 pKa = 10.76GISSPDD38 pKa = 2.81WCTWDD43 pKa = 4.17EE44 pKa = 4.42LDD46 pKa = 4.16PNCEE50 pKa = 3.64ILHH53 pKa = 6.25FEE55 pKa = 4.99NEE57 pKa = 3.78DD58 pKa = 4.65DD59 pKa = 4.8INNLCIYY66 pKa = 9.9EE67 pKa = 4.34NYY69 pKa = 10.89DD70 pKa = 3.75FIDD73 pKa = 3.51EE74 pKa = 4.21LRR76 pKa = 11.84LYY78 pKa = 9.89TEE80 pKa = 3.62NKK82 pKa = 7.92YY83 pKa = 10.75IYY85 pKa = 9.87MIDD88 pKa = 3.68CLTDD92 pKa = 3.24KK93 pKa = 10.79KK94 pKa = 10.64RR95 pKa = 11.84AEE97 pKa = 3.81QLFDD101 pKa = 4.04YY102 pKa = 10.36ICSINLLNEE111 pKa = 3.43ICIYY115 pKa = 10.69

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371ISH8|A0A371ISH8_9FIRM Protein jag OS=Romboutsia maritimum OX=2020948 GN=CHF27_008045 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2679

0

2679

797421

17

1444

297.7

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.07 ± 0.047

1.202 ± 0.02

5.713 ± 0.041

7.239 ± 0.055

4.211 ± 0.039

6.075 ± 0.043

1.291 ± 0.017

10.609 ± 0.067

9.622 ± 0.052

9.062 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.023

6.796 ± 0.048

2.593 ± 0.024

2.443 ± 0.022

3.014 ± 0.033

6.461 ± 0.044

4.848 ± 0.037

6.396 ± 0.036

0.558 ± 0.015

4.164 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski