Streptococcus phage Javan573

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AJ46|A0A4D6AJ46_9CAUD Uncharacterized protein OS=Streptococcus phage Javan573 OX=2548266 GN=Javan573_0016 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.78NLLAEE7 pKa = 4.59WADD10 pKa = 3.5QCPITVKK17 pKa = 10.83LKK19 pKa = 10.32DD20 pKa = 3.26STVIVFDD27 pKa = 5.26GILDD31 pKa = 3.67VHH33 pKa = 7.18DD34 pKa = 4.79DD35 pKa = 4.29CIEE38 pKa = 4.08LLEE41 pKa = 5.3KK42 pKa = 10.3DD43 pKa = 3.4TGDD46 pKa = 2.99IYY48 pKa = 10.74ILQKK52 pKa = 10.95EE53 pKa = 4.73DD54 pKa = 3.25ILFAKK59 pKa = 9.94INSSDD64 pKa = 3.31IPEE67 pKa = 3.95AA68 pKa = 4.27

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ANM9|A0A4D6ANM9_9CAUD HNHc domain-containing protein OS=Streptococcus phage Javan573 OX=2548266 GN=Javan573_0015 PE=4 SV=1
MM1 pKa = 7.47SIVEE5 pKa = 4.25KK6 pKa = 11.03VRR8 pKa = 11.84TALRR12 pKa = 11.84NKK14 pKa = 9.92PNTTAKK20 pKa = 10.0EE21 pKa = 3.59ISVALNIKK29 pKa = 9.86VGLVRR34 pKa = 11.84IAMNRR39 pKa = 11.84LKK41 pKa = 10.59KK42 pKa = 10.26AQQSS46 pKa = 3.44

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11573

46

1436

206.7

23.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.535 ± 0.488

0.406 ± 0.081

6.351 ± 0.354

7.543 ± 0.489

3.586 ± 0.174

6.299 ± 0.385

1.426 ± 0.138

6.999 ± 0.284

8.235 ± 0.517

7.759 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.191

5.452 ± 0.181

2.653 ± 0.178

4.251 ± 0.218

4.649 ± 0.295

6.049 ± 0.379

6.36 ± 0.647

6.826 ± 0.352

1.339 ± 0.193

3.888 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski