Synchytrium endobioticum
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7895 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A507DGB9|A0A507DGB9_9FUNG WD_REPEATS_REGION domain-containing protein OS=Synchytrium endobioticum OX=286115 GN=SeLEV6574_g01199 PE=4 SV=1
MM1 pKa = 7.15 LTLRR5 pKa = 11.84 QTVVEE10 pKa = 4.37 LIIMVMLMSSNVLLSPVPMGACFSSTRR37 pKa = 11.84 GSAEE41 pKa = 3.85 VVAPDD46 pKa = 3.59 YY47 pKa = 11.33 PNDD50 pKa = 3.27 VVAAHH55 pKa = 5.68 YY56 pKa = 10.52 HH57 pKa = 5.61 NNDD60 pKa = 3.27 DD61 pKa = 4.32 RR62 pKa = 11.84 TDD64 pKa = 3.39 SSDD67 pKa = 3.57 DD68 pKa = 3.45 LRR70 pKa = 11.84 GVNIPFSDD78 pKa = 4.02 GEE80 pKa = 4.23 TDD82 pKa = 3.49 GPEE85 pKa = 4.9 DD86 pKa = 3.84 YY87 pKa = 10.4 FSPSSDD93 pKa = 2.85 PVVSTEE99 pKa = 3.98 VSSDD103 pKa = 3.16 VCMTICQSRR112 pKa = 11.84 STSTVTGSVSLVV124 pKa = 3.14
Molecular weight: 13.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.808
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.846
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.882
Protein with the highest isoelectric point:
>tr|A0A507DSE5|A0A507DSE5_9FUNG RRM domain-containing protein OS=Synchytrium endobioticum OX=286115 GN=SeMB42_g00139 PE=3 SV=1
MM1 pKa = 7.47 LRR3 pKa = 11.84 AQHH6 pKa = 6.33 EE7 pKa = 4.51 PTVQRR12 pKa = 11.84 SPTISIITPKK22 pKa = 9.73 MLSEE26 pKa = 3.93 RR27 pKa = 11.84 ATLNRR32 pKa = 11.84 HH33 pKa = 4.71 PAKK36 pKa = 10.16 PKK38 pKa = 10.55 APTVPLSRR46 pKa = 11.84 TPARR50 pKa = 11.84 TPVALSASTSISSTRR65 pKa = 11.84 AAPARR70 pKa = 11.84 SAPARR75 pKa = 11.84 SEE77 pKa = 3.76 NCATSKK83 pKa = 11.14 ASFASTPRR91 pKa = 11.84 FSSAVTGRR99 pKa = 11.84 EE100 pKa = 3.65 RR101 pKa = 11.84 LTGKK105 pKa = 9.53 HH106 pKa = 5.78 APAATLRR113 pKa = 11.84 AAIQEE118 pKa = 4.01 PFMGKK123 pKa = 8.23 NHH125 pKa = 5.53 STAFGTVYY133 pKa = 10.05 TRR135 pKa = 11.84 GGIPCRR141 pKa = 11.84 LQHH144 pKa = 6.73 GSVQHH149 pKa = 6.71 RR150 pKa = 11.84 IQWTTSPPSLSFPTLLPLFIVGLLEE175 pKa = 3.55 THH177 pKa = 5.87 PQYY180 pKa = 11.23 RR181 pKa = 11.84 FIAHH185 pKa = 6.06 QGLMEE190 pKa = 5.59 CISAPNCGDD199 pKa = 4.38 SIQQAWTVAVPPLRR213 pKa = 11.84 GVLGNSGDD221 pKa = 3.57 KK222 pKa = 10.98 GALLTALKK230 pKa = 10.1 ALAAMIPNLSIDD242 pKa = 4.18 SLLSACGMLLPPLSRR257 pKa = 11.84 LAVSKK262 pKa = 10.83 DD263 pKa = 3.24 RR264 pKa = 11.84 EE265 pKa = 4.52 VNDD268 pKa = 3.79 ATHH271 pKa = 6.47 SMLRR275 pKa = 11.84 TLEE278 pKa = 3.98 EE279 pKa = 3.77 RR280 pKa = 11.84 ALEE283 pKa = 4.02 YY284 pKa = 10.38 PQVLKK289 pKa = 10.79 LIKK292 pKa = 10.36 ARR294 pKa = 11.84 IPTYY298 pKa = 10.31 TSIRR302 pKa = 11.84 SCC304 pKa = 3.89
Molecular weight: 32.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.589
IPC_protein 10.321
Toseland 10.716
ProMoST 10.394
Dawson 10.789
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 11.008
Grimsley 10.833
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.716
IPC_peptide 10.921
IPC2_peptide 9.706
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7895
0
7895
3746747
8
4874
474.6
52.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.44 ± 0.028
1.393 ± 0.011
5.793 ± 0.018
5.644 ± 0.027
3.382 ± 0.014
5.737 ± 0.021
2.729 ± 0.013
5.205 ± 0.016
5.153 ± 0.023
9.192 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.01
4.019 ± 0.015
5.774 ± 0.027
3.972 ± 0.019
6.02 ± 0.02
8.705 ± 0.032
6.081 ± 0.019
6.409 ± 0.018
1.144 ± 0.008
2.817 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here