Vibrio phage phi-A318

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Kaohsiungvirus; Vibrio virus A318

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067YCN9|A0A067YCN9_9CAUD Uncharacterized protein OS=Vibrio phage phi-A318 OX=1151014 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.38KK4 pKa = 10.72LSFNDD9 pKa = 3.48WLEE12 pKa = 4.29LYY14 pKa = 10.2QDD16 pKa = 4.09EE17 pKa = 4.89LPEE20 pKa = 4.5EE21 pKa = 4.86GDD23 pKa = 3.77LYY25 pKa = 10.56PLYY28 pKa = 10.57EE29 pKa = 4.63EE30 pKa = 4.46YY31 pKa = 11.06LEE33 pKa = 4.35SVVDD37 pKa = 4.33AEE39 pKa = 5.62IEE41 pKa = 3.95TALINKK47 pKa = 7.18MHH49 pKa = 6.88

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067YAT9|A0A067YAT9_9CAUD Uncharacterized protein OS=Vibrio phage phi-A318 OX=1151014 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.3VDD5 pKa = 3.46EE6 pKa = 4.3KK7 pKa = 11.41LPRR10 pKa = 11.84KK11 pKa = 9.12KK12 pKa = 9.88HH13 pKa = 4.2NHH15 pKa = 5.41KK16 pKa = 10.01KK17 pKa = 9.58AHH19 pKa = 5.42EE20 pKa = 4.45TNSSRR25 pKa = 11.84NRR27 pKa = 11.84KK28 pKa = 9.56RR29 pKa = 11.84IRR31 pKa = 11.84EE32 pKa = 4.05MKK34 pKa = 10.46RR35 pKa = 11.84GWCNN39 pKa = 2.77

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12557

38

1229

273.0

30.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.465 ± 0.556

1.011 ± 0.151

6.443 ± 0.198

7.685 ± 0.444

3.807 ± 0.201

6.873 ± 0.334

2.031 ± 0.21

5.439 ± 0.217

7.303 ± 0.283

8.067 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.188

4.89 ± 0.177

3.544 ± 0.198

4.014 ± 0.256

5.16 ± 0.304

5.742 ± 0.345

5.248 ± 0.332

6.132 ± 0.308

1.354 ± 0.154

3.719 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski