Amino acid dipepetide frequency for Vibrio phage phi-A318

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.318AlaAla: 9.318 ± 1.835
0.876AlaCys: 0.876 ± 0.269
5.814AlaAsp: 5.814 ± 0.514
6.531AlaGlu: 6.531 ± 0.953
3.026AlaPhe: 3.026 ± 0.47
5.655AlaGly: 5.655 ± 0.59
1.991AlaHis: 1.991 ± 0.581
5.336AlaIle: 5.336 ± 0.783
5.655AlaLys: 5.655 ± 0.684
6.133AlaLeu: 6.133 ± 0.759
2.788AlaMet: 2.788 ± 0.534
3.504AlaAsn: 3.504 ± 0.566
3.265AlaPro: 3.265 ± 0.537
3.106AlaGln: 3.106 ± 0.49
4.699AlaArg: 4.699 ± 0.854
3.903AlaSer: 3.903 ± 0.762
3.345AlaThr: 3.345 ± 0.591
5.416AlaVal: 5.416 ± 0.672
0.876AlaTrp: 0.876 ± 0.323
3.345AlaTyr: 3.345 ± 0.506
0.0AlaXaa: 0.0 ± 0.0
Cys
0.398CysAla: 0.398 ± 0.175
0.0CysCys: 0.0 ± 0.0
0.239CysAsp: 0.239 ± 0.115
0.956CysGlu: 0.956 ± 0.354
0.239CysPhe: 0.239 ± 0.119
0.717CysGly: 0.717 ± 0.304
0.239CysHis: 0.239 ± 0.129
0.717CysIle: 0.717 ± 0.254
0.717CysLys: 0.717 ± 0.22
0.876CysLeu: 0.876 ± 0.301
0.239CysMet: 0.239 ± 0.144
0.558CysAsn: 0.558 ± 0.207
0.558CysPro: 0.558 ± 0.223
0.319CysGln: 0.319 ± 0.145
0.717CysArg: 0.717 ± 0.24
0.637CysSer: 0.637 ± 0.255
0.478CysThr: 0.478 ± 0.209
0.717CysVal: 0.717 ± 0.25
0.239CysTrp: 0.239 ± 0.122
0.558CysTyr: 0.558 ± 0.17
0.0CysXaa: 0.0 ± 0.0
Asp
6.292AspAla: 6.292 ± 0.736
0.717AspCys: 0.717 ± 0.223
3.823AspAsp: 3.823 ± 0.434
4.38AspGlu: 4.38 ± 0.705
2.867AspPhe: 2.867 ± 0.425
4.54AspGly: 4.54 ± 0.879
0.876AspHis: 0.876 ± 0.227
3.982AspIle: 3.982 ± 0.737
5.814AspLys: 5.814 ± 0.682
4.619AspLeu: 4.619 ± 0.673
1.274AspMet: 1.274 ± 0.39
3.345AspAsn: 3.345 ± 0.518
2.549AspPro: 2.549 ± 0.491
1.035AspGln: 1.035 ± 0.345
2.708AspArg: 2.708 ± 0.381
3.186AspSer: 3.186 ± 0.563
3.425AspThr: 3.425 ± 0.588
4.858AspVal: 4.858 ± 0.615
1.274AspTrp: 1.274 ± 0.424
2.867AspTyr: 2.867 ± 0.417
0.0AspXaa: 0.0 ± 0.0
Glu
6.212GluAla: 6.212 ± 0.98
0.796GluCys: 0.796 ± 0.305
4.46GluAsp: 4.46 ± 0.724
8.283GluGlu: 8.283 ± 1.183
3.425GluPhe: 3.425 ± 0.512
5.256GluGly: 5.256 ± 0.531
1.513GluHis: 1.513 ± 0.403
3.345GluIle: 3.345 ± 0.475
4.619GluLys: 4.619 ± 0.625
6.849GluLeu: 6.849 ± 0.83
2.708GluMet: 2.708 ± 0.59
2.628GluAsn: 2.628 ± 0.36
2.469GluPro: 2.469 ± 0.513
4.141GluGln: 4.141 ± 0.562
3.903GluArg: 3.903 ± 0.49
4.46GluSer: 4.46 ± 0.805
2.628GluThr: 2.628 ± 0.288
4.619GluVal: 4.619 ± 0.759
1.673GluTrp: 1.673 ± 0.401
2.867GluTyr: 2.867 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
3.026PheAla: 3.026 ± 0.47
0.478PheCys: 0.478 ± 0.197
3.186PheAsp: 3.186 ± 0.684
2.549PheGlu: 2.549 ± 0.455
1.035PhePhe: 1.035 ± 0.276
2.15PheGly: 2.15 ± 0.384
0.876PheHis: 0.876 ± 0.221
2.389PheIle: 2.389 ± 0.407
2.628PheLys: 2.628 ± 0.445
2.469PheLeu: 2.469 ± 0.391
1.673PheMet: 1.673 ± 0.381
2.31PheAsn: 2.31 ± 0.3
1.513PhePro: 1.513 ± 0.363
1.115PheGln: 1.115 ± 0.229
2.23PheArg: 2.23 ± 0.309
2.549PheSer: 2.549 ± 0.394
1.911PheThr: 1.911 ± 0.375
2.389PheVal: 2.389 ± 0.419
0.558PheTrp: 0.558 ± 0.207
1.035PheTyr: 1.035 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
4.779GlyAla: 4.779 ± 0.744
0.956GlyCys: 0.956 ± 0.317
4.54GlyAsp: 4.54 ± 0.548
5.734GlyGlu: 5.734 ± 0.644
2.23GlyPhe: 2.23 ± 0.343
5.336GlyGly: 5.336 ± 0.784
1.593GlyHis: 1.593 ± 0.431
3.823GlyIle: 3.823 ± 0.618
7.646GlyLys: 7.646 ± 0.756
4.858GlyLeu: 4.858 ± 0.777
2.23GlyMet: 2.23 ± 0.298
3.026GlyAsn: 3.026 ± 0.491
0.0GlyPro: 0.0 ± 0.0
2.15GlyGln: 2.15 ± 0.38
3.345GlyArg: 3.345 ± 0.467
4.141GlySer: 4.141 ± 0.694
4.38GlyThr: 4.38 ± 0.933
3.504GlyVal: 3.504 ± 0.645
1.195GlyTrp: 1.195 ± 0.306
3.265GlyTyr: 3.265 ± 0.56
0.0GlyXaa: 0.0 ± 0.0
His
1.354HisAla: 1.354 ± 0.401
0.239HisCys: 0.239 ± 0.155
1.593HisAsp: 1.593 ± 0.303
1.354HisGlu: 1.354 ± 0.338
0.717HisPhe: 0.717 ± 0.227
1.195HisGly: 1.195 ± 0.341
0.796HisHis: 0.796 ± 0.281
1.274HisIle: 1.274 ± 0.283
1.434HisLys: 1.434 ± 0.359
2.31HisLeu: 2.31 ± 0.469
0.398HisMet: 0.398 ± 0.136
1.274HisAsn: 1.274 ± 0.36
0.637HisPro: 0.637 ± 0.276
1.035HisGln: 1.035 ± 0.262
0.637HisArg: 0.637 ± 0.183
1.274HisSer: 1.274 ± 0.288
0.876HisThr: 0.876 ± 0.312
1.195HisVal: 1.195 ± 0.351
0.159HisTrp: 0.159 ± 0.105
0.558HisTyr: 0.558 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
4.46IleAla: 4.46 ± 0.827
0.398IleCys: 0.398 ± 0.174
4.141IleAsp: 4.141 ± 0.515
3.823IleGlu: 3.823 ± 0.533
1.593IlePhe: 1.593 ± 0.44
4.38IleGly: 4.38 ± 0.522
1.354IleHis: 1.354 ± 0.349
3.106IleIle: 3.106 ± 0.633
5.177IleLys: 5.177 ± 0.781
3.026IleLeu: 3.026 ± 0.449
1.274IleMet: 1.274 ± 0.276
2.788IleAsn: 2.788 ± 0.626
1.911IlePro: 1.911 ± 0.356
2.31IleGln: 2.31 ± 0.518
2.708IleArg: 2.708 ± 0.498
2.708IleSer: 2.708 ± 0.555
3.425IleThr: 3.425 ± 0.67
3.186IleVal: 3.186 ± 0.47
0.796IleTrp: 0.796 ± 0.274
1.832IleTyr: 1.832 ± 0.521
0.0IleXaa: 0.0 ± 0.0
Lys
7.009LysAla: 7.009 ± 0.92
0.717LysCys: 0.717 ± 0.22
5.575LysAsp: 5.575 ± 0.766
6.371LysGlu: 6.371 ± 0.732
2.628LysPhe: 2.628 ± 0.359
3.982LysGly: 3.982 ± 0.628
1.593LysHis: 1.593 ± 0.349
1.911LysIle: 1.911 ± 0.355
4.619LysLys: 4.619 ± 0.696
6.531LysLeu: 6.531 ± 0.675
2.15LysMet: 2.15 ± 0.459
3.504LysAsn: 3.504 ± 0.515
3.186LysPro: 3.186 ± 0.505
3.664LysGln: 3.664 ± 0.396
4.54LysArg: 4.54 ± 0.744
3.425LysSer: 3.425 ± 0.513
4.141LysThr: 4.141 ± 0.548
3.743LysVal: 3.743 ± 0.656
0.717LysTrp: 0.717 ± 0.221
3.026LysTyr: 3.026 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
6.849LeuAla: 6.849 ± 0.706
0.239LeuCys: 0.239 ± 0.152
5.894LeuAsp: 5.894 ± 0.623
6.133LeuGlu: 6.133 ± 0.746
2.389LeuPhe: 2.389 ± 0.364
5.495LeuGly: 5.495 ± 0.499
2.15LeuHis: 2.15 ± 0.496
4.619LeuIle: 4.619 ± 0.597
5.097LeuLys: 5.097 ± 0.631
5.734LeuLeu: 5.734 ± 0.747
2.788LeuMet: 2.788 ± 0.35
3.026LeuAsn: 3.026 ± 0.522
3.823LeuPro: 3.823 ± 0.533
3.345LeuGln: 3.345 ± 0.528
4.619LeuArg: 4.619 ± 0.812
4.54LeuSer: 4.54 ± 0.763
4.38LeuThr: 4.38 ± 0.475
5.734LeuVal: 5.734 ± 0.724
0.717LeuTrp: 0.717 ± 0.225
3.106LeuTyr: 3.106 ± 0.484
0.0LeuXaa: 0.0 ± 0.0
Met
2.708MetAla: 2.708 ± 0.355
0.08MetCys: 0.08 ± 0.074
1.115MetAsp: 1.115 ± 0.327
2.071MetGlu: 2.071 ± 0.429
1.274MetPhe: 1.274 ± 0.344
1.752MetGly: 1.752 ± 0.453
0.717MetHis: 0.717 ± 0.213
1.195MetIle: 1.195 ± 0.274
2.389MetLys: 2.389 ± 0.403
3.265MetLeu: 3.265 ± 0.615
0.558MetMet: 0.558 ± 0.284
1.354MetAsn: 1.354 ± 0.272
1.434MetPro: 1.434 ± 0.343
1.673MetGln: 1.673 ± 0.342
2.071MetArg: 2.071 ± 0.375
2.389MetSer: 2.389 ± 0.488
2.23MetThr: 2.23 ± 0.454
1.195MetVal: 1.195 ± 0.28
0.08MetTrp: 0.08 ± 0.073
1.195MetTyr: 1.195 ± 0.286
0.0MetXaa: 0.0 ± 0.0
Asn
3.743AsnAla: 3.743 ± 0.66
0.159AsnCys: 0.159 ± 0.102
2.947AsnAsp: 2.947 ± 0.52
2.389AsnGlu: 2.389 ± 0.363
1.911AsnPhe: 1.911 ± 0.289
3.743AsnGly: 3.743 ± 0.675
0.717AsnHis: 0.717 ± 0.243
2.788AsnIle: 2.788 ± 0.595
4.38AsnLys: 4.38 ± 0.591
5.336AsnLeu: 5.336 ± 0.659
1.354AsnMet: 1.354 ± 0.308
2.867AsnAsn: 2.867 ± 0.469
2.23AsnPro: 2.23 ± 0.45
1.752AsnGln: 1.752 ± 0.349
2.15AsnArg: 2.15 ± 0.42
2.867AsnSer: 2.867 ± 0.43
2.628AsnThr: 2.628 ± 0.407
2.788AsnVal: 2.788 ± 0.525
0.637AsnTrp: 0.637 ± 0.235
1.513AsnTyr: 1.513 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
2.947ProAla: 2.947 ± 0.469
0.478ProCys: 0.478 ± 0.179
2.469ProAsp: 2.469 ± 0.491
4.062ProGlu: 4.062 ± 0.664
1.593ProPhe: 1.593 ± 0.364
0.239ProGly: 0.239 ± 0.143
0.398ProHis: 0.398 ± 0.154
2.23ProIle: 2.23 ± 0.44
1.911ProLys: 1.911 ± 0.385
3.106ProLeu: 3.106 ± 0.436
1.035ProMet: 1.035 ± 0.195
2.389ProAsn: 2.389 ± 0.343
1.035ProPro: 1.035 ± 0.287
1.434ProGln: 1.434 ± 0.289
1.354ProArg: 1.354 ± 0.434
2.708ProSer: 2.708 ± 0.453
1.752ProThr: 1.752 ± 0.381
2.469ProVal: 2.469 ± 0.459
0.637ProTrp: 0.637 ± 0.229
1.195ProTyr: 1.195 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
4.301GlnAla: 4.301 ± 0.552
0.398GlnCys: 0.398 ± 0.149
1.354GlnAsp: 1.354 ± 0.284
3.823GlnGlu: 3.823 ± 0.656
1.832GlnPhe: 1.832 ± 0.314
2.15GlnGly: 2.15 ± 0.476
0.558GlnHis: 0.558 ± 0.2
1.752GlnIle: 1.752 ± 0.328
1.513GlnLys: 1.513 ± 0.356
3.265GlnLeu: 3.265 ± 0.539
1.434GlnMet: 1.434 ± 0.263
2.389GlnAsn: 2.389 ± 0.326
1.035GlnPro: 1.035 ± 0.314
2.628GlnGln: 2.628 ± 0.576
2.708GlnArg: 2.708 ± 0.678
2.867GlnSer: 2.867 ± 0.564
1.832GlnThr: 1.832 ± 0.422
2.628GlnVal: 2.628 ± 0.41
0.319GlnTrp: 0.319 ± 0.167
1.354GlnTyr: 1.354 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
4.221ArgAla: 4.221 ± 0.712
0.398ArgCys: 0.398 ± 0.189
3.345ArgAsp: 3.345 ± 0.487
3.504ArgGlu: 3.504 ± 0.477
1.593ArgPhe: 1.593 ± 0.307
3.982ArgGly: 3.982 ± 0.598
0.796ArgHis: 0.796 ± 0.242
2.947ArgIle: 2.947 ± 0.464
4.141ArgLys: 4.141 ± 0.625
3.982ArgLeu: 3.982 ± 0.487
1.752ArgMet: 1.752 ± 0.329
2.708ArgAsn: 2.708 ± 0.62
1.593ArgPro: 1.593 ± 0.399
1.991ArgGln: 1.991 ± 0.43
2.867ArgArg: 2.867 ± 0.502
2.389ArgSer: 2.389 ± 0.413
2.788ArgThr: 2.788 ± 0.406
3.504ArgVal: 3.504 ± 0.393
1.035ArgTrp: 1.035 ± 0.218
2.071ArgTyr: 2.071 ± 0.473
0.0ArgXaa: 0.0 ± 0.0
Ser
3.982SerAla: 3.982 ± 0.647
0.796SerCys: 0.796 ± 0.253
2.947SerAsp: 2.947 ± 0.383
3.982SerGlu: 3.982 ± 0.578
2.549SerPhe: 2.549 ± 0.543
5.097SerGly: 5.097 ± 0.774
0.956SerHis: 0.956 ± 0.203
3.982SerIle: 3.982 ± 0.527
4.46SerLys: 4.46 ± 0.611
5.177SerLeu: 5.177 ± 0.579
2.15SerMet: 2.15 ± 0.473
2.788SerAsn: 2.788 ± 0.574
1.911SerPro: 1.911 ± 0.422
1.911SerGln: 1.911 ± 0.388
1.911SerArg: 1.911 ± 0.382
3.345SerSer: 3.345 ± 0.658
3.106SerThr: 3.106 ± 0.633
3.345SerVal: 3.345 ± 0.659
0.717SerTrp: 0.717 ± 0.256
2.31SerTyr: 2.31 ± 0.583
0.0SerXaa: 0.0 ± 0.0
Thr
3.903ThrAla: 3.903 ± 0.535
0.637ThrCys: 0.637 ± 0.212
3.664ThrAsp: 3.664 ± 0.525
4.141ThrGlu: 4.141 ± 0.595
2.628ThrPhe: 2.628 ± 0.509
4.779ThrGly: 4.779 ± 0.764
0.876ThrHis: 0.876 ± 0.274
3.106ThrIle: 3.106 ± 0.44
3.106ThrLys: 3.106 ± 0.55
4.221ThrLeu: 4.221 ± 0.583
1.354ThrMet: 1.354 ± 0.33
2.31ThrAsn: 2.31 ± 0.371
2.071ThrPro: 2.071 ± 0.441
2.23ThrGln: 2.23 ± 0.387
2.31ThrArg: 2.31 ± 0.396
2.628ThrSer: 2.628 ± 0.75
3.265ThrThr: 3.265 ± 0.556
3.186ThrVal: 3.186 ± 0.403
0.398ThrTrp: 0.398 ± 0.193
1.673ThrTyr: 1.673 ± 0.345
0.0ThrXaa: 0.0 ± 0.0
Val
5.575ValAla: 5.575 ± 0.845
0.478ValCys: 0.478 ± 0.2
4.38ValAsp: 4.38 ± 0.657
3.425ValGlu: 3.425 ± 0.609
2.389ValPhe: 2.389 ± 0.572
4.779ValGly: 4.779 ± 0.547
1.035ValHis: 1.035 ± 0.309
3.504ValIle: 3.504 ± 0.558
4.38ValLys: 4.38 ± 0.565
4.779ValLeu: 4.779 ± 0.684
1.911ValMet: 1.911 ± 0.468
3.265ValAsn: 3.265 ± 0.533
2.15ValPro: 2.15 ± 0.385
1.991ValGln: 1.991 ± 0.424
3.664ValArg: 3.664 ± 0.571
3.584ValSer: 3.584 ± 0.77
3.504ValThr: 3.504 ± 0.724
4.699ValVal: 4.699 ± 0.593
0.478ValTrp: 0.478 ± 0.215
1.354ValTyr: 1.354 ± 0.504
0.0ValXaa: 0.0 ± 0.0
Trp
1.035TrpAla: 1.035 ± 0.29
0.637TrpCys: 0.637 ± 0.275
0.478TrpAsp: 0.478 ± 0.155
1.035TrpGlu: 1.035 ± 0.335
0.558TrpPhe: 0.558 ± 0.237
0.717TrpGly: 0.717 ± 0.202
0.398TrpHis: 0.398 ± 0.175
0.558TrpIle: 0.558 ± 0.245
1.115TrpLys: 1.115 ± 0.366
1.274TrpLeu: 1.274 ± 0.397
0.637TrpMet: 0.637 ± 0.196
0.558TrpAsn: 0.558 ± 0.172
0.239TrpPro: 0.239 ± 0.145
0.637TrpGln: 0.637 ± 0.226
0.239TrpArg: 0.239 ± 0.125
1.115TrpSer: 1.115 ± 0.296
0.717TrpThr: 0.717 ± 0.26
0.478TrpVal: 0.478 ± 0.176
0.239TrpTrp: 0.239 ± 0.174
0.876TrpTyr: 0.876 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.549TyrAla: 2.549 ± 0.489
0.637TyrCys: 0.637 ± 0.223
2.469TyrAsp: 2.469 ± 0.385
2.31TyrGlu: 2.31 ± 0.406
1.593TyrPhe: 1.593 ± 0.39
2.788TyrGly: 2.788 ± 0.423
0.876TyrHis: 0.876 ± 0.275
1.832TyrIle: 1.832 ± 0.351
2.23TyrLys: 2.23 ± 0.331
2.867TyrLeu: 2.867 ± 0.345
0.956TyrMet: 0.956 ± 0.268
2.31TyrAsn: 2.31 ± 0.356
1.832TyrPro: 1.832 ± 0.438
1.673TyrGln: 1.673 ± 0.381
2.23TyrArg: 2.23 ± 0.411
2.708TyrSer: 2.708 ± 0.559
1.673TyrThr: 1.673 ± 0.372
1.673TyrVal: 1.673 ± 0.353
0.796TyrTrp: 0.796 ± 0.28
1.195TyrTyr: 1.195 ± 0.299
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (12557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski