Bacteroidales bacterium Barb4

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A180F610|A0A180F610_9BACT Copper homeostasis protein CutC OS=Bacteroidales bacterium Barb4 OX=1633200 GN=cutC PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.63NEE4 pKa = 3.98AQHH7 pKa = 6.82LIDD10 pKa = 4.63RR11 pKa = 11.84FLSARR16 pKa = 11.84DD17 pKa = 3.66EE18 pKa = 4.13GRR20 pKa = 11.84EE21 pKa = 4.0AYY23 pKa = 10.29FDD25 pKa = 3.95ADD27 pKa = 4.41EE28 pKa = 5.68IDD30 pKa = 5.41DD31 pKa = 4.36LLEE34 pKa = 4.34GCEE37 pKa = 4.16DD38 pKa = 4.24KK39 pKa = 11.37EE40 pKa = 5.27DD41 pKa = 3.82FDD43 pKa = 5.86LYY45 pKa = 11.38EE46 pKa = 4.26EE47 pKa = 4.32VLALGLKK54 pKa = 9.13LHH56 pKa = 7.11PEE58 pKa = 4.33NIGLQTKK65 pKa = 9.86RR66 pKa = 11.84CWQYY70 pKa = 11.12VVEE73 pKa = 4.48EE74 pKa = 4.47NYY76 pKa = 7.72TTALEE81 pKa = 4.59LIEE84 pKa = 4.7QIGATGDD91 pKa = 3.54QEE93 pKa = 4.21IEE95 pKa = 4.05AVQLEE100 pKa = 5.14CYY102 pKa = 10.62LMLDD106 pKa = 3.52MTDD109 pKa = 3.25EE110 pKa = 4.56AEE112 pKa = 4.27ALSEE116 pKa = 4.18KK117 pKa = 9.97MIAEE121 pKa = 4.3GRR123 pKa = 11.84EE124 pKa = 4.03SMDD127 pKa = 3.76LVFEE131 pKa = 4.61YY132 pKa = 10.18IVPLLNDD139 pKa = 3.11KK140 pKa = 10.7EE141 pKa = 4.44MYY143 pKa = 10.43SLALEE148 pKa = 4.23YY149 pKa = 11.05VNRR152 pKa = 11.84GLEE155 pKa = 4.33LYY157 pKa = 9.93PDD159 pKa = 3.69SLVLKK164 pKa = 10.56EE165 pKa = 4.13EE166 pKa = 4.38LCFLMEE172 pKa = 3.99VAEE175 pKa = 4.55DD176 pKa = 3.79LEE178 pKa = 4.72GAIDD182 pKa = 4.26LCNAMIDD189 pKa = 3.83EE190 pKa = 5.04DD191 pKa = 4.66PYY193 pKa = 11.43SYY195 pKa = 10.78EE196 pKa = 3.66YY197 pKa = 10.55WFTLGRR203 pKa = 11.84LYY205 pKa = 11.1SLTEE209 pKa = 4.01EE210 pKa = 4.18FEE212 pKa = 4.21KK213 pKa = 11.1AIDD216 pKa = 3.9AFDD219 pKa = 4.04FAQTCEE225 pKa = 4.32EE226 pKa = 4.56PDD228 pKa = 4.07SEE230 pKa = 5.08LKK232 pKa = 10.63LLKK235 pKa = 10.16AYY237 pKa = 10.56CFFMSDD243 pKa = 3.26KK244 pKa = 11.15YY245 pKa = 11.13EE246 pKa = 4.06EE247 pKa = 4.33ALILYY252 pKa = 8.44TEE254 pKa = 4.26LLKK257 pKa = 11.14DD258 pKa = 3.57EE259 pKa = 4.9EE260 pKa = 4.76KK261 pKa = 11.02NGEE264 pKa = 4.23DD265 pKa = 4.64GEE267 pKa = 4.47GDD269 pKa = 3.41NYY271 pKa = 10.24ILEE274 pKa = 4.32RR275 pKa = 11.84VRR277 pKa = 11.84PLLAEE282 pKa = 4.56CYY284 pKa = 8.12MKK286 pKa = 11.06LFDD289 pKa = 5.07YY290 pKa = 10.03EE291 pKa = 3.82AAYY294 pKa = 10.48LILHH298 pKa = 6.42EE299 pKa = 5.64LIADD303 pKa = 3.89DD304 pKa = 5.38AEE306 pKa = 4.34AAVYY310 pKa = 9.55EE311 pKa = 4.41ADD313 pKa = 4.62DD314 pKa = 4.63AGTEE318 pKa = 3.94ADD320 pKa = 4.2EE321 pKa = 4.85TDD323 pKa = 3.62TDD325 pKa = 3.98DD326 pKa = 4.06TGAAQKK332 pKa = 10.59DD333 pKa = 3.98YY334 pKa = 11.38SPYY337 pKa = 9.71IYY339 pKa = 10.9YY340 pKa = 7.49MHH342 pKa = 7.46CCAQTDD348 pKa = 3.98RR349 pKa = 11.84KK350 pKa = 10.24QDD352 pKa = 3.48ALDD355 pKa = 4.04ALEE358 pKa = 4.25TAATVFDD365 pKa = 4.58GSAHH369 pKa = 5.66VLSLMAYY376 pKa = 9.82AYY378 pKa = 9.66MEE380 pKa = 4.27SAEE383 pKa = 4.29NDD385 pKa = 3.58DD386 pKa = 4.75NLDD389 pKa = 3.78VINKK393 pKa = 9.28LFEE396 pKa = 5.87LIDD399 pKa = 4.32DD400 pKa = 5.06PGDD403 pKa = 4.15DD404 pKa = 5.02LEE406 pKa = 4.85QDD408 pKa = 3.38EE409 pKa = 5.55DD410 pKa = 4.36DD411 pKa = 4.73PSGEE415 pKa = 4.25SDD417 pKa = 3.45FSEE420 pKa = 4.65TVGKK424 pKa = 8.97MLEE427 pKa = 4.2YY428 pKa = 10.32FKK430 pKa = 10.7KK431 pKa = 10.29IYY433 pKa = 9.9AANPDD438 pKa = 3.25QPYY441 pKa = 10.56INLYY445 pKa = 10.09LATAYY450 pKa = 10.62LINDD454 pKa = 3.48DD455 pKa = 3.65MQNFEE460 pKa = 3.9KK461 pKa = 10.44HH462 pKa = 5.65YY463 pKa = 9.78NLLSAEE469 pKa = 4.34EE470 pKa = 4.08LDD472 pKa = 5.01SYY474 pKa = 10.26AKK476 pKa = 10.26KK477 pKa = 10.91YY478 pKa = 9.94NAGDD482 pKa = 3.44SRR484 pKa = 11.84ALRR487 pKa = 11.84TVAEE491 pKa = 4.04KK492 pKa = 10.68HH493 pKa = 5.61IGLRR497 pKa = 11.84DD498 pKa = 3.35LAEE501 pKa = 4.63EE502 pKa = 4.16YY503 pKa = 10.61LSKK506 pKa = 10.93PEE508 pKa = 3.94NSNN511 pKa = 3.08

Molecular weight: 58.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A180FCT2|A0A180FCT2_9BACT Multidrug-efflux transporter OS=Bacteroidales bacterium Barb4 OX=1633200 GN=mdtK PE=4 SV=1
MM1 pKa = 7.5SLLLRR6 pKa = 11.84LLLLLLRR13 pKa = 11.84LLRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LLRR21 pKa = 11.84LFRR24 pKa = 11.84LLRR27 pKa = 11.84LIRR30 pKa = 11.84LSLLMLLRR38 pKa = 11.84LIRR41 pKa = 11.84LRR43 pKa = 11.84LLVRR47 pKa = 11.84LRR49 pKa = 11.84LII51 pKa = 3.96

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3840

0

3840

958267

29

2482

249.5

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.952 ± 0.048

1.299 ± 0.022

5.314 ± 0.026

6.142 ± 0.042

4.797 ± 0.03

7.139 ± 0.052

1.999 ± 0.022

6.63 ± 0.04

6.196 ± 0.045

9.156 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.029

4.758 ± 0.038

3.967 ± 0.03

3.227 ± 0.027

5.175 ± 0.042

6.395 ± 0.043

5.814 ± 0.045

6.634 ± 0.04

1.038 ± 0.015

4.033 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski