Amino acid dipepetide frequency for Bacteroidales bacterium Barb4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.742AlaAla: 7.742 ± 0.122	1.193AlaCys: 1.193 ± 0.04	4.987AlaAsp: 4.987 ± 0.079	5.43AlaGlu: 5.43 ± 0.09	4.04AlaPhe: 4.04 ± 0.095	6.727AlaGly: 6.727 ± 0.108	1.3AlaHis: 1.3 ± 0.041	4.162AlaIle: 4.162 ± 0.078	4.361AlaLys: 4.361 ± 0.079	7.213AlaLeu: 7.213 ± 0.104	1.624AlaMet: 1.624 ± 0.044	3.268AlaAsn: 3.268 ± 0.072	2.387AlaPro: 2.387 ± 0.052	2.418AlaGln: 2.418 ± 0.048	3.493AlaArg: 3.493 ± 0.064	4.73AlaSer: 4.73 ± 0.083	3.861AlaThr: 3.861 ± 0.082	6.838AlaVal: 6.838 ± 0.106	0.74AlaTrp: 0.74 ± 0.027	3.011AlaTyr: 3.011 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.858CysAla: 0.858 ± 0.03	0.278CysCys: 0.278 ± 0.019	0.533CysAsp: 0.533 ± 0.026	0.574CysGlu: 0.574 ± 0.029	0.776CysPhe: 0.776 ± 0.032	1.076CysGly: 1.076 ± 0.037	0.37CysHis: 0.37 ± 0.02	0.85CysIle: 0.85 ± 0.032	0.716CysLys: 0.716 ± 0.029	1.284CysLeu: 1.284 ± 0.037	0.335CysMet: 0.335 ± 0.018	0.512CysAsn: 0.512 ± 0.026	0.717CysPro: 0.717 ± 0.029	0.363CysGln: 0.363 ± 0.021	0.916CysArg: 0.916 ± 0.036	0.773CysSer: 0.773 ± 0.034	0.76CysThr: 0.76 ± 0.035	0.702CysVal: 0.702 ± 0.025	0.131CysTrp: 0.131 ± 0.012	0.464CysTyr: 0.464 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.16AspAla: 4.16 ± 0.07	0.69AspCys: 0.69 ± 0.029	2.609AspAsp: 2.609 ± 0.063	3.366AspGlu: 3.366 ± 0.069	2.937AspPhe: 2.937 ± 0.056	4.095AspGly: 4.095 ± 0.08	0.727AspHis: 0.727 ± 0.029	4.052AspIle: 4.052 ± 0.067	3.552AspLys: 3.552 ± 0.072	4.503AspLeu: 4.503 ± 0.072	1.358AspMet: 1.358 ± 0.038	2.806AspAsn: 2.806 ± 0.05	1.836AspPro: 1.836 ± 0.047	0.945AspGln: 0.945 ± 0.036	2.721AspArg: 2.721 ± 0.059	3.056AspSer: 3.056 ± 0.077	2.967AspThr: 2.967 ± 0.063	3.377AspVal: 3.377 ± 0.063	0.674AspTrp: 0.674 ± 0.026	2.712AspTyr: 2.712 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.406GluAla: 5.406 ± 0.096	0.586GluCys: 0.586 ± 0.032	2.96GluAsp: 2.96 ± 0.065	4.495GluGlu: 4.495 ± 0.085	2.131GluPhe: 2.131 ± 0.046	4.111GluGly: 4.111 ± 0.069	1.202GluHis: 1.202 ± 0.038	4.311GluIle: 4.311 ± 0.071	4.649GluLys: 4.649 ± 0.074	5.575GluLeu: 5.575 ± 0.085	1.612GluMet: 1.612 ± 0.047	3.043GluAsn: 3.043 ± 0.058	1.691GluPro: 1.691 ± 0.05	2.356GluGln: 2.356 ± 0.05	3.431GluArg: 3.431 ± 0.066	3.216GluSer: 3.216 ± 0.054	3.691GluThr: 3.691 ± 0.059	3.797GluVal: 3.797 ± 0.073	0.762GluTrp: 0.762 ± 0.029	2.395GluTyr: 2.395 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.997PheAla: 3.997 ± 0.081	0.679PheCys: 0.679 ± 0.027	2.817PheAsp: 2.817 ± 0.052	2.593PheGlu: 2.593 ± 0.054	2.413PhePhe: 2.413 ± 0.063	3.498PheGly: 3.498 ± 0.072	1.035PheHis: 1.035 ± 0.039	3.142PheIle: 3.142 ± 0.068	2.241PheLys: 2.241 ± 0.052	4.473PheLeu: 4.473 ± 0.086	1.1PheMet: 1.1 ± 0.036	2.26PheAsn: 2.26 ± 0.054	1.99PhePro: 1.99 ± 0.044	1.57PheGln: 1.57 ± 0.046	2.663PheArg: 2.663 ± 0.061	3.329PheSer: 3.329 ± 0.073	2.953PheThr: 2.953 ± 0.071	2.968PheVal: 2.968 ± 0.064	0.456PheTrp: 0.456 ± 0.024	1.797PheTyr: 1.797 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.646GlyAla: 4.646 ± 0.078	1.03GlyCys: 1.03 ± 0.043	3.875GlyAsp: 3.875 ± 0.09	4.326GlyGlu: 4.326 ± 0.073	3.584GlyPhe: 3.584 ± 0.06	5.722GlyGly: 5.722 ± 0.134	1.324GlyHis: 1.324 ± 0.04	5.682GlyIle: 5.682 ± 0.092	5.197GlyLys: 5.197 ± 0.078	6.042GlyLeu: 6.042 ± 0.096	1.961GlyMet: 1.961 ± 0.048	3.79GlyAsn: 3.79 ± 0.075	0.879GlyPro: 0.879 ± 0.035	2.098GlyGln: 2.098 ± 0.04	3.542GlyArg: 3.542 ± 0.07	4.298GlySer: 4.298 ± 0.079	4.454GlyThr: 4.454 ± 0.092	4.723GlyVal: 4.723 ± 0.082	0.834GlyTrp: 0.834 ± 0.034	3.387GlyTyr: 3.387 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	1.364HisAla: 1.364 ± 0.042	0.31HisCys: 0.31 ± 0.019	0.833HisAsp: 0.833 ± 0.029	0.915HisGlu: 0.915 ± 0.035	1.25HisPhe: 1.25 ± 0.045	1.314HisGly: 1.314 ± 0.037	0.507HisHis: 0.507 ± 0.024	1.724HisIle: 1.724 ± 0.038	1.033HisLys: 1.033 ± 0.038	1.992HisLeu: 1.992 ± 0.045	0.346HisMet: 0.346 ± 0.019	0.853HisAsn: 0.853 ± 0.027	1.137HisPro: 1.137 ± 0.04	0.545HisGln: 0.545 ± 0.024	1.091HisArg: 1.091 ± 0.038	1.352HisSer: 1.352 ± 0.043	1.193HisThr: 1.193 ± 0.034	1.203HisVal: 1.203 ± 0.041	0.201HisTrp: 0.201 ± 0.014	0.823HisTyr: 0.823 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.327IleAla: 5.327 ± 0.087	0.858IleCys: 0.858 ± 0.032	3.961IleAsp: 3.961 ± 0.077	4.154IleGlu: 4.154 ± 0.063	2.602IlePhe: 2.602 ± 0.054	4.53IleGly: 4.53 ± 0.071	1.32IleHis: 1.32 ± 0.037	4.4IleIle: 4.4 ± 0.094	3.913IleLys: 3.913 ± 0.07	5.602IleLeu: 5.602 ± 0.102	1.268IleMet: 1.268 ± 0.04	3.251IleAsn: 3.251 ± 0.06	3.324IlePro: 3.324 ± 0.07	2.086IleGln: 2.086 ± 0.048	3.797IleArg: 3.797 ± 0.071	4.419IleSer: 4.419 ± 0.068	4.13IleThr: 4.13 ± 0.076	4.338IleVal: 4.338 ± 0.074	0.529IleTrp: 0.529 ± 0.025	2.489IleTyr: 2.489 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	4.975LysAla: 4.975 ± 0.083	0.521LysCys: 0.521 ± 0.025	3.615LysAsp: 3.615 ± 0.068	5.054LysGlu: 5.054 ± 0.095	1.992LysPhe: 1.992 ± 0.046	4.562LysGly: 4.562 ± 0.082	1.256LysHis: 1.256 ± 0.039	3.896LysIle: 3.896 ± 0.079	4.319LysLys: 4.319 ± 0.082	4.827LysLeu: 4.827 ± 0.071	1.626LysMet: 1.626 ± 0.043	3.091LysAsn: 3.091 ± 0.056	2.252LysPro: 2.252 ± 0.053	2.389LysGln: 2.389 ± 0.056	3.313LysArg: 3.313 ± 0.062	3.768LysSer: 3.768 ± 0.073	3.551LysThr: 3.551 ± 0.067	3.787LysVal: 3.787 ± 0.066	0.766LysTrp: 0.766 ± 0.036	2.395LysTyr: 2.395 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	6.462LeuAla: 6.462 ± 0.086	1.366LeuCys: 1.366 ± 0.04	4.28LeuAsp: 4.28 ± 0.075	4.738LeuGlu: 4.738 ± 0.087	4.812LeuPhe: 4.812 ± 0.095	5.067LeuGly: 5.067 ± 0.085	2.219LeuHis: 2.219 ± 0.055	5.467LeuIle: 5.467 ± 0.08	6.208LeuLys: 6.208 ± 0.08	9.128LeuLeu: 9.128 ± 0.138	2.115LeuMet: 2.115 ± 0.046	4.34LeuAsn: 4.34 ± 0.069	4.657LeuPro: 4.657 ± 0.083	3.297LeuGln: 3.297 ± 0.065	4.85LeuArg: 4.85 ± 0.088	7.455LeuSer: 7.455 ± 0.091	5.897LeuThr: 5.897 ± 0.1	4.866LeuVal: 4.866 ± 0.081	0.831LeuTrp: 0.831 ± 0.035	3.502LeuTyr: 3.502 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	1.744MetAla: 1.744 ± 0.045	0.238MetCys: 0.238 ± 0.016	1.152MetAsp: 1.152 ± 0.037	1.6MetGlu: 1.6 ± 0.046	0.867MetPhe: 0.867 ± 0.029	1.452MetGly: 1.452 ± 0.044	0.423MetHis: 0.423 ± 0.022	1.441MetIle: 1.441 ± 0.039	1.945MetLys: 1.945 ± 0.039	2.171MetLeu: 2.171 ± 0.049	0.602MetMet: 0.602 ± 0.025	1.206MetAsn: 1.206 ± 0.036	1.187MetPro: 1.187 ± 0.034	1.006MetGln: 1.006 ± 0.038	1.314MetArg: 1.314 ± 0.041	1.32MetSer: 1.32 ± 0.04	1.372MetThr: 1.372 ± 0.033	1.312MetVal: 1.312 ± 0.036	0.258MetTrp: 0.258 ± 0.016	0.747MetTyr: 0.747 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.762AsnAla: 3.762 ± 0.074	0.518AsnCys: 0.518 ± 0.033	2.316AsnAsp: 2.316 ± 0.06	2.675AsnGlu: 2.675 ± 0.054	1.971AsnPhe: 1.971 ± 0.051	3.641AsnGly: 3.641 ± 0.076	0.896AsnHis: 0.896 ± 0.033	3.524AsnIle: 3.524 ± 0.057	2.786AsnLys: 2.786 ± 0.055	4.057AsnLeu: 4.057 ± 0.074	1.095AsnMet: 1.095 ± 0.036	2.464AsnAsn: 2.464 ± 0.058	2.689AsnPro: 2.689 ± 0.055	1.324AsnGln: 1.324 ± 0.047	2.603AsnArg: 2.603 ± 0.058	2.842AsnSer: 2.842 ± 0.059	2.825AsnThr: 2.825 ± 0.063	3.011AsnVal: 3.011 ± 0.06	0.493AsnTrp: 0.493 ± 0.023	2.094AsnTyr: 2.094 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	3.705ProAla: 3.705 ± 0.08	0.548ProCys: 0.548 ± 0.029	2.828ProAsp: 2.828 ± 0.055	3.085ProGlu: 3.085 ± 0.056	2.186ProPhe: 2.186 ± 0.058	2.054ProGly: 2.054 ± 0.049	1.03ProHis: 1.03 ± 0.035	1.892ProIle: 1.892 ± 0.046	1.951ProLys: 1.951 ± 0.057	3.601ProLeu: 3.601 ± 0.078	0.79ProMet: 0.79 ± 0.031	1.68ProAsn: 1.68 ± 0.043	1.397ProPro: 1.397 ± 0.054	1.333ProGln: 1.333 ± 0.039	1.475ProArg: 1.475 ± 0.039	2.611ProSer: 2.611 ± 0.055	1.853ProThr: 1.853 ± 0.045	3.601ProVal: 3.601 ± 0.077	0.363ProTrp: 0.363 ± 0.023	1.686ProTyr: 1.686 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	2.612GlnAla: 2.612 ± 0.063	0.315GlnCys: 0.315 ± 0.017	1.385GlnAsp: 1.385 ± 0.038	2.064GlnGlu: 2.064 ± 0.04	1.276GlnPhe: 1.276 ± 0.044	2.112GlnGly: 2.112 ± 0.049	0.653GlnHis: 0.653 ± 0.03	2.058GlnIle: 2.058 ± 0.048	2.194GlnLys: 2.194 ± 0.058	2.997GlnLeu: 2.997 ± 0.059	0.922GlnMet: 0.922 ± 0.031	1.477GlnAsn: 1.477 ± 0.042	1.342GlnPro: 1.342 ± 0.044	1.424GlnGln: 1.424 ± 0.042	1.767GlnArg: 1.767 ± 0.049	2.021GlnSer: 2.021 ± 0.052	2.234GlnThr: 2.234 ± 0.044	1.792GlnVal: 1.792 ± 0.046	0.381GlnTrp: 0.381 ± 0.021	1.247GlnTyr: 1.247 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	3.325ArgAla: 3.325 ± 0.061	0.651ArgCys: 0.651 ± 0.029	2.259ArgAsp: 2.259 ± 0.052	3.26ArgGlu: 3.26 ± 0.066	2.735ArgPhe: 2.735 ± 0.058	2.812ArgGly: 2.812 ± 0.064	1.212ArgHis: 1.212 ± 0.039	4.018ArgIle: 4.018 ± 0.072	3.524ArgLys: 3.524 ± 0.07	5.227ArgLeu: 5.227 ± 0.095	1.545ArgMet: 1.545 ± 0.041	2.629ArgAsn: 2.629 ± 0.058	1.959ArgPro: 1.959 ± 0.054	2.198ArgGln: 2.198 ± 0.053	2.979ArgArg: 2.979 ± 0.079	2.855ArgSer: 2.855 ± 0.064	2.873ArgThr: 2.873 ± 0.055	2.889ArgVal: 2.889 ± 0.061	0.58ArgTrp: 0.58 ± 0.029	2.215ArgTyr: 2.215 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	5.196SerAla: 5.196 ± 0.091	0.951SerCys: 0.951 ± 0.032	3.446SerAsp: 3.446 ± 0.062	3.288SerGlu: 3.288 ± 0.065	3.524SerPhe: 3.524 ± 0.072	5.414SerGly: 5.414 ± 0.101	1.172SerHis: 1.172 ± 0.04	4.125SerIle: 4.125 ± 0.093	3.236SerLys: 3.236 ± 0.053	6.609SerLeu: 6.609 ± 0.094	1.288SerMet: 1.288 ± 0.034	2.683SerAsn: 2.683 ± 0.065	2.743SerPro: 2.743 ± 0.058	1.677SerGln: 1.677 ± 0.05	3.256SerArg: 3.256 ± 0.065	4.28SerSer: 4.28 ± 0.093	2.976SerThr: 2.976 ± 0.072	4.888SerVal: 4.888 ± 0.088	0.65SerTrp: 0.65 ± 0.027	2.546SerTyr: 2.546 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	5.685ThrAla: 5.685 ± 0.115	0.591ThrCys: 0.591 ± 0.023	3.685ThrAsp: 3.685 ± 0.067	3.41ThrGlu: 3.41 ± 0.069	2.971ThrPhe: 2.971 ± 0.064	5.201ThrGly: 5.201 ± 0.091	0.967ThrHis: 0.967 ± 0.032	3.748ThrIle: 3.748 ± 0.079	2.702ThrLys: 2.702 ± 0.057	5.522ThrLeu: 5.522 ± 0.093	1.069ThrMet: 1.069 ± 0.035	2.314ThrAsn: 2.314 ± 0.06	2.712ThrPro: 2.712 ± 0.053	1.574ThrGln: 1.574 ± 0.043	2.188ThrArg: 2.188 ± 0.049	3.294ThrSer: 3.294 ± 0.065	2.913ThrThr: 2.913 ± 0.069	5.055ThrVal: 5.055 ± 0.113	0.508ThrTrp: 0.508 ± 0.024	2.027ThrTyr: 2.027 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.835ValAla: 4.835 ± 0.066	1.024ValCys: 1.024 ± 0.037	2.777ValAsp: 2.777 ± 0.06	3.627ValGlu: 3.627 ± 0.054	3.413ValPhe: 3.413 ± 0.065	4.125ValGly: 4.125 ± 0.075	1.228ValHis: 1.228 ± 0.039	4.55ValIle: 4.55 ± 0.072	4.317ValLys: 4.317 ± 0.073	6.175ValLeu: 6.175 ± 0.098	1.478ValMet: 1.478 ± 0.044	3.185ValAsn: 3.185 ± 0.073	2.78ValPro: 2.78 ± 0.058	1.979ValGln: 1.979 ± 0.046	3.551ValArg: 3.551 ± 0.063	5.15ValSer: 5.15 ± 0.085	4.363ValThr: 4.363 ± 0.112	4.536ValVal: 4.536 ± 0.084	0.704ValTrp: 0.704 ± 0.03	2.543ValTyr: 2.543 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.667TrpAla: 0.667 ± 0.03	0.121TrpCys: 0.121 ± 0.011	0.567TrpAsp: 0.567 ± 0.026	0.665TrpGlu: 0.665 ± 0.03	0.465TrpPhe: 0.465 ± 0.019	0.89TrpGly: 0.89 ± 0.033	0.242TrpHis: 0.242 ± 0.015	0.595TrpIle: 0.595 ± 0.027	0.727TrpLys: 0.727 ± 0.029	0.986TrpLeu: 0.986 ± 0.034	0.285TrpMet: 0.285 ± 0.016	0.547TrpAsn: 0.547 ± 0.024	0.176TrpPro: 0.176 ± 0.016	0.5TrpGln: 0.5 ± 0.021	0.519TrpArg: 0.519 ± 0.024	0.6TrpSer: 0.6 ± 0.023	0.713TrpThr: 0.713 ± 0.037	0.564TrpVal: 0.564 ± 0.025	0.118TrpTrp: 0.118 ± 0.012	0.433TrpTyr: 0.433 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.059TyrAla: 3.059 ± 0.061	0.524TyrCys: 0.524 ± 0.026	2.259TyrAsp: 2.259 ± 0.055	2.101TyrGlu: 2.101 ± 0.051	2.028TyrPhe: 2.028 ± 0.053	2.99TyrGly: 2.99 ± 0.063	0.902TyrHis: 0.902 ± 0.033	2.662TyrIle: 2.662 ± 0.054	2.383TyrLys: 2.383 ± 0.043	3.573TyrLeu: 3.573 ± 0.066	0.936TyrMet: 0.936 ± 0.03	2.186TyrAsn: 2.186 ± 0.054	1.815TyrPro: 1.815 ± 0.048	1.194TyrGln: 1.194 ± 0.034	2.277TyrArg: 2.277 ± 0.054	2.577TyrSer: 2.577 ± 0.063	2.557TyrThr: 2.557 ± 0.055	2.094TyrVal: 2.094 ± 0.051	0.4TyrTrp: 0.4 ± 0.024	1.812TyrTyr: 1.812 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3840 proteins (958267 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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