Amino acid dipepetide frequency for Pelagibacterium luteolum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.079AlaAla: 15.079 ± 0.157	0.844AlaCys: 0.844 ± 0.031	6.925AlaAsp: 6.925 ± 0.071	7.519AlaGlu: 7.519 ± 0.098	4.389AlaPhe: 4.389 ± 0.062	9.726AlaGly: 9.726 ± 0.107	2.331AlaHis: 2.331 ± 0.046	7.498AlaIle: 7.498 ± 0.087	3.71AlaLys: 3.71 ± 0.07	13.529AlaLeu: 13.529 ± 0.134	3.716AlaMet: 3.716 ± 0.057	3.189AlaAsn: 3.189 ± 0.061	5.37AlaPro: 5.37 ± 0.085	4.41AlaGln: 4.41 ± 0.074	8.029AlaArg: 8.029 ± 0.102	6.369AlaSer: 6.369 ± 0.07	6.068AlaThr: 6.068 ± 0.083	8.374AlaVal: 8.374 ± 0.093	1.36AlaTrp: 1.36 ± 0.032	2.563AlaTyr: 2.563 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.888CysAla: 0.888 ± 0.026	0.092CysCys: 0.092 ± 0.009	0.466CysAsp: 0.466 ± 0.021	0.372CysGlu: 0.372 ± 0.017	0.26CysPhe: 0.26 ± 0.016	0.749CysGly: 0.749 ± 0.026	0.204CysHis: 0.204 ± 0.013	0.353CysIle: 0.353 ± 0.017	0.179CysLys: 0.179 ± 0.011	0.676CysLeu: 0.676 ± 0.024	0.125CysMet: 0.125 ± 0.01	0.194CysAsn: 0.194 ± 0.012	0.388CysPro: 0.388 ± 0.019	0.208CysGln: 0.208 ± 0.013	0.492CysArg: 0.492 ± 0.021	0.369CysSer: 0.369 ± 0.018	0.365CysThr: 0.365 ± 0.016	0.523CysVal: 0.523 ± 0.023	0.09CysTrp: 0.09 ± 0.009	0.181CysTyr: 0.181 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.435AspAla: 7.435 ± 0.086	0.42AspCys: 0.42 ± 0.019	3.517AspAsp: 3.517 ± 0.07	3.701AspGlu: 3.701 ± 0.059	2.315AspPhe: 2.315 ± 0.036	5.331AspGly: 5.331 ± 0.088	1.309AspHis: 1.309 ± 0.036	3.663AspIle: 3.663 ± 0.053	1.733AspLys: 1.733 ± 0.042	6.064AspLeu: 6.064 ± 0.077	1.469AspMet: 1.469 ± 0.037	1.507AspAsn: 1.507 ± 0.035	3.365AspPro: 3.365 ± 0.064	1.833AspGln: 1.833 ± 0.04	4.275AspArg: 4.275 ± 0.063	2.247AspSer: 2.247 ± 0.04	3.059AspThr: 3.059 ± 0.056	4.165AspVal: 4.165 ± 0.061	1.051AspTrp: 1.051 ± 0.033	1.503AspTyr: 1.503 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.571GluAla: 7.571 ± 0.096	0.308GluCys: 0.308 ± 0.017	3.298GluAsp: 3.298 ± 0.056	3.169GluGlu: 3.169 ± 0.063	1.893GluPhe: 1.893 ± 0.038	4.453GluGly: 4.453 ± 0.065	1.238GluHis: 1.238 ± 0.032	3.899GluIle: 3.899 ± 0.054	2.267GluLys: 2.267 ± 0.05	5.348GluLeu: 5.348 ± 0.068	1.705GluMet: 1.705 ± 0.038	1.751GluAsn: 1.751 ± 0.042	2.767GluPro: 2.767 ± 0.066	2.159GluGln: 2.159 ± 0.044	4.828GluArg: 4.828 ± 0.073	2.561GluSer: 2.561 ± 0.048	4.007GluThr: 4.007 ± 0.069	3.89GluVal: 3.89 ± 0.061	0.728GluTrp: 0.728 ± 0.024	1.048GluTyr: 1.048 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.902PheAla: 4.902 ± 0.063	0.366PheCys: 0.366 ± 0.017	2.773PheAsp: 2.773 ± 0.052	2.426PheGlu: 2.426 ± 0.046	1.53PhePhe: 1.53 ± 0.038	3.871PheGly: 3.871 ± 0.063	0.678PheHis: 0.678 ± 0.025	1.904PheIle: 1.904 ± 0.044	0.997PheLys: 0.997 ± 0.031	3.302PheLeu: 3.302 ± 0.057	0.861PheMet: 0.861 ± 0.024	1.118PheAsn: 1.118 ± 0.032	1.568PhePro: 1.568 ± 0.037	0.975PheGln: 0.975 ± 0.027	2.128PheArg: 2.128 ± 0.041	2.508PheSer: 2.508 ± 0.047	2.022PheThr: 2.022 ± 0.041	2.948PheVal: 2.948 ± 0.051	0.584PheTrp: 0.584 ± 0.024	0.949PheTyr: 0.949 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	8.892GlyAla: 8.892 ± 0.117	0.694GlyCys: 0.694 ± 0.022	4.451GlyAsp: 4.451 ± 0.061	4.957GlyGlu: 4.957 ± 0.068	3.644GlyPhe: 3.644 ± 0.053	6.767GlyGly: 6.767 ± 0.083	1.871GlyHis: 1.871 ± 0.037	4.893GlyIle: 4.893 ± 0.075	3.006GlyLys: 3.006 ± 0.057	8.818GlyLeu: 8.818 ± 0.107	2.314GlyMet: 2.314 ± 0.048	2.343GlyAsn: 2.343 ± 0.06	3.383GlyPro: 3.383 ± 0.053	3.055GlyGln: 3.055 ± 0.05	5.574GlyArg: 5.574 ± 0.082	4.526GlySer: 4.526 ± 0.067	4.688GlyThr: 4.688 ± 0.064	6.111GlyVal: 6.111 ± 0.077	1.343GlyTrp: 1.343 ± 0.04	2.268GlyTyr: 2.268 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.225HisAla: 2.225 ± 0.045	0.199HisCys: 0.199 ± 0.013	1.212HisAsp: 1.212 ± 0.037	1.131HisGlu: 1.131 ± 0.03	0.898HisPhe: 0.898 ± 0.024	1.814HisGly: 1.814 ± 0.038	0.581HisHis: 0.581 ± 0.023	1.13HisIle: 1.13 ± 0.032	0.565HisLys: 0.565 ± 0.019	2.033HisLeu: 2.033 ± 0.039	0.501HisMet: 0.501 ± 0.02	0.511HisAsn: 0.511 ± 0.019	1.241HisPro: 1.241 ± 0.032	0.654HisGln: 0.654 ± 0.02	1.338HisArg: 1.338 ± 0.036	0.954HisSer: 0.954 ± 0.029	0.911HisThr: 0.911 ± 0.024	1.44HisVal: 1.44 ± 0.033	0.323HisTrp: 0.323 ± 0.016	0.555HisTyr: 0.555 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.399IleAla: 8.399 ± 0.097	0.484IleCys: 0.484 ± 0.019	4.143IleAsp: 4.143 ± 0.06	4.239IleGlu: 4.239 ± 0.064	2.014IlePhe: 2.014 ± 0.044	5.381IleGly: 5.381 ± 0.07	0.987IleHis: 0.987 ± 0.027	2.85IleIle: 2.85 ± 0.062	1.521IleLys: 1.521 ± 0.029	4.868IleLeu: 4.868 ± 0.075	1.211IleMet: 1.211 ± 0.029	1.561IleAsn: 1.561 ± 0.034	2.5IlePro: 2.5 ± 0.047	1.303IleGln: 1.303 ± 0.034	3.104IleArg: 3.104 ± 0.051	3.342IleSer: 3.342 ± 0.052	3.096IleThr: 3.096 ± 0.05	4.945IleVal: 4.945 ± 0.072	0.684IleTrp: 0.684 ± 0.024	1.212IleTyr: 1.212 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.822LysAla: 3.822 ± 0.066	0.144LysCys: 0.144 ± 0.012	1.581LysAsp: 1.581 ± 0.037	1.293LysGlu: 1.293 ± 0.041	0.983LysPhe: 0.983 ± 0.028	2.515LysGly: 2.515 ± 0.053	0.614LysHis: 0.614 ± 0.024	1.757LysIle: 1.757 ± 0.04	1.163LysLys: 1.163 ± 0.038	3.094LysLeu: 3.094 ± 0.054	0.748LysMet: 0.748 ± 0.026	0.716LysAsn: 0.716 ± 0.027	1.823LysPro: 1.823 ± 0.041	0.89LysGln: 0.89 ± 0.027	2.386LysArg: 2.386 ± 0.044	2.063LysSer: 2.063 ± 0.042	1.978LysThr: 1.978 ± 0.045	2.162LysVal: 2.162 ± 0.052	0.367LysTrp: 0.367 ± 0.017	0.53LysTyr: 0.53 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.176LeuAla: 13.176 ± 0.137	0.736LeuCys: 0.736 ± 0.023	6.434LeuAsp: 6.434 ± 0.079	5.712LeuGlu: 5.712 ± 0.067	3.678LeuPhe: 3.678 ± 0.06	8.321LeuGly: 8.321 ± 0.098	1.723LeuHis: 1.723 ± 0.034	5.608LeuIle: 5.608 ± 0.077	3.291LeuLys: 3.291 ± 0.053	8.934LeuLeu: 8.934 ± 0.106	2.444LeuMet: 2.444 ± 0.04	2.699LeuAsn: 2.699 ± 0.047	5.121LeuPro: 5.121 ± 0.08	2.535LeuGln: 2.535 ± 0.044	6.214LeuArg: 6.214 ± 0.074	6.619LeuSer: 6.619 ± 0.08	5.62LeuThr: 5.62 ± 0.069	7.59LeuVal: 7.59 ± 0.086	1.113LeuTrp: 1.113 ± 0.034	2.096LeuTyr: 2.096 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.328MetAla: 3.328 ± 0.054	0.142MetCys: 0.142 ± 0.01	1.232MetAsp: 1.232 ± 0.038	1.131MetGlu: 1.131 ± 0.031	0.799MetPhe: 0.799 ± 0.026	2.038MetGly: 2.038 ± 0.05	0.426MetHis: 0.426 ± 0.018	1.565MetIle: 1.565 ± 0.04	0.835MetLys: 0.835 ± 0.028	2.637MetLeu: 2.637 ± 0.047	0.709MetMet: 0.709 ± 0.025	0.737MetAsn: 0.737 ± 0.025	1.555MetPro: 1.555 ± 0.038	0.762MetGln: 0.762 ± 0.024	1.753MetArg: 1.753 ± 0.035	1.836MetSer: 1.836 ± 0.041	2.018MetThr: 2.018 ± 0.042	1.975MetVal: 1.975 ± 0.044	0.193MetTrp: 0.193 ± 0.012	0.318MetTyr: 0.318 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.416AsnAla: 3.416 ± 0.056	0.232AsnCys: 0.232 ± 0.013	1.521AsnAsp: 1.521 ± 0.035	1.376AsnGlu: 1.376 ± 0.033	1.123AsnPhe: 1.123 ± 0.033	2.514AsnGly: 2.514 ± 0.058	0.538AsnHis: 0.538 ± 0.024	1.473AsnIle: 1.473 ± 0.037	0.667AsnLys: 0.667 ± 0.026	2.687AsnLeu: 2.687 ± 0.042	0.646AsnMet: 0.646 ± 0.024	0.766AsnAsn: 0.766 ± 0.029	1.867AsnPro: 1.867 ± 0.044	0.877AsnGln: 0.877 ± 0.03	1.813AsnArg: 1.813 ± 0.041	1.39AsnSer: 1.39 ± 0.033	1.42AsnThr: 1.42 ± 0.037	2.006AsnVal: 2.006 ± 0.052	0.472AsnTrp: 0.472 ± 0.023	0.703AsnTyr: 0.703 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.454ProAla: 5.454 ± 0.081	0.278ProCys: 0.278 ± 0.017	3.772ProAsp: 3.772 ± 0.056	3.789ProGlu: 3.789 ± 0.064	2.023ProPhe: 2.023 ± 0.038	3.999ProGly: 3.999 ± 0.064	1.085ProHis: 1.085 ± 0.032	2.727ProIle: 2.727 ± 0.049	1.578ProLys: 1.578 ± 0.045	4.489ProLeu: 4.489 ± 0.066	1.238ProMet: 1.238 ± 0.028	1.455ProAsn: 1.455 ± 0.033	2.118ProPro: 2.118 ± 0.054	1.731ProGln: 1.731 ± 0.034	2.638ProArg: 2.638 ± 0.051	2.83ProSer: 2.83 ± 0.052	2.616ProThr: 2.616 ± 0.05	4.029ProVal: 4.029 ± 0.063	0.629ProTrp: 0.629 ± 0.025	1.157ProTyr: 1.157 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.771GlnAla: 3.771 ± 0.065	0.186GlnCys: 0.186 ± 0.013	1.605GlnAsp: 1.605 ± 0.037	1.406GlnGlu: 1.406 ± 0.034	1.25GlnPhe: 1.25 ± 0.03	2.303GlnGly: 2.303 ± 0.045	0.614GlnHis: 0.614 ± 0.023	2.066GlnIle: 2.066 ± 0.046	1.013GlnLys: 1.013 ± 0.029	3.037GlnLeu: 3.037 ± 0.057	1.065GlnMet: 1.065 ± 0.03	0.914GlnAsn: 0.914 ± 0.029	1.779GlnPro: 1.779 ± 0.038	1.238GlnGln: 1.238 ± 0.034	2.443GlnArg: 2.443 ± 0.044	2.116GlnSer: 2.116 ± 0.042	1.976GlnThr: 1.976 ± 0.041	2.202GlnVal: 2.202 ± 0.042	0.44GlnTrp: 0.44 ± 0.02	0.676GlnTyr: 0.676 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.411ArgAla: 7.411 ± 0.096	0.422ArgCys: 0.422 ± 0.016	4.083ArgAsp: 4.083 ± 0.066	4.113ArgGlu: 4.113 ± 0.063	2.849ArgPhe: 2.849 ± 0.046	4.22ArgGly: 4.22 ± 0.068	1.574ArgHis: 1.574 ± 0.037	3.844ArgIle: 3.844 ± 0.059	2.254ArgLys: 2.254 ± 0.041	7.108ArgLeu: 7.108 ± 0.093	1.763ArgMet: 1.763 ± 0.038	1.884ArgAsn: 1.884 ± 0.042	3.2ArgPro: 3.2 ± 0.055	2.631ArgGln: 2.631 ± 0.053	4.93ArgArg: 4.93 ± 0.084	3.557ArgSer: 3.557 ± 0.049	3.411ArgThr: 3.411 ± 0.06	4.485ArgVal: 4.485 ± 0.07	0.957ArgTrp: 0.957 ± 0.03	1.717ArgTyr: 1.717 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.434SerAla: 6.434 ± 0.082	0.356SerCys: 0.356 ± 0.019	3.346SerAsp: 3.346 ± 0.055	3.086SerGlu: 3.086 ± 0.055	2.284SerPhe: 2.284 ± 0.045	5.655SerGly: 5.655 ± 0.077	1.167SerHis: 1.167 ± 0.029	3.145SerIle: 3.145 ± 0.052	1.495SerLys: 1.495 ± 0.037	5.405SerLeu: 5.405 ± 0.066	1.452SerMet: 1.452 ± 0.037	1.567SerAsn: 1.567 ± 0.035	2.683SerPro: 2.683 ± 0.044	1.886SerGln: 1.886 ± 0.047	3.677SerArg: 3.677 ± 0.054	3.142SerSer: 3.142 ± 0.053	2.908SerThr: 2.908 ± 0.057	4.264SerVal: 4.264 ± 0.06	0.736SerTrp: 0.736 ± 0.027	1.282SerTyr: 1.282 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.163ThrAla: 6.163 ± 0.07	0.379ThrCys: 0.379 ± 0.019	3.162ThrAsp: 3.162 ± 0.054	2.955ThrGlu: 2.955 ± 0.048	1.957ThrPhe: 1.957 ± 0.041	5.13ThrGly: 5.13 ± 0.074	1.148ThrHis: 1.148 ± 0.031	3.501ThrIle: 3.501 ± 0.05	1.401ThrLys: 1.401 ± 0.039	6.288ThrLeu: 6.288 ± 0.069	1.292ThrMet: 1.292 ± 0.034	1.505ThrAsn: 1.505 ± 0.041	3.418ThrPro: 3.418 ± 0.057	1.653ThrGln: 1.653 ± 0.039	3.386ThrArg: 3.386 ± 0.048	2.983ThrSer: 2.983 ± 0.047	3.078ThrThr: 3.078 ± 0.058	4.34ThrVal: 4.34 ± 0.058	0.604ThrTrp: 0.604 ± 0.02	1.28ThrTyr: 1.28 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.847ValAla: 8.847 ± 0.094	0.57ValCys: 0.57 ± 0.021	4.297ValAsp: 4.297 ± 0.067	4.668ValGlu: 4.668 ± 0.069	2.998ValPhe: 2.998 ± 0.049	5.846ValGly: 5.846 ± 0.079	1.357ValHis: 1.357 ± 0.037	4.31ValIle: 4.31 ± 0.066	2.039ValLys: 2.039 ± 0.052	7.488ValLeu: 7.488 ± 0.092	1.93ValMet: 1.93 ± 0.044	2.011ValAsn: 2.011 ± 0.045	3.705ValPro: 3.705 ± 0.064	1.982ValGln: 1.982 ± 0.043	4.644ValArg: 4.644 ± 0.064	4.514ValSer: 4.514 ± 0.058	4.492ValThr: 4.492 ± 0.063	5.798ValVal: 5.798 ± 0.076	0.879ValTrp: 0.879 ± 0.029	1.493ValTyr: 1.493 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.305TrpAla: 1.305 ± 0.036	0.124TrpCys: 0.124 ± 0.01	0.699TrpAsp: 0.699 ± 0.022	0.547TrpGlu: 0.547 ± 0.022	0.54TrpPhe: 0.54 ± 0.021	0.889TrpGly: 0.889 ± 0.03	0.313TrpHis: 0.313 ± 0.016	0.684TrpIle: 0.684 ± 0.023	0.342TrpLys: 0.342 ± 0.019	1.566TrpLeu: 1.566 ± 0.042	0.345TrpMet: 0.345 ± 0.015	0.442TrpAsn: 0.442 ± 0.018	0.683TrpPro: 0.683 ± 0.025	0.563TrpGln: 0.563 ± 0.019	1.075TrpArg: 1.075 ± 0.031	0.848TrpSer: 0.848 ± 0.028	0.761TrpThr: 0.761 ± 0.025	0.897TrpVal: 0.897 ± 0.026	0.217TrpTrp: 0.217 ± 0.013	0.273TrpTyr: 0.273 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.479TyrAla: 2.479 ± 0.045	0.201TyrCys: 0.201 ± 0.012	1.447TyrAsp: 1.447 ± 0.034	1.298TyrGlu: 1.298 ± 0.031	0.98TyrPhe: 0.98 ± 0.025	2.074TyrGly: 2.074 ± 0.042	0.459TyrHis: 0.459 ± 0.016	0.976TyrIle: 0.976 ± 0.028	0.573TyrLys: 0.573 ± 0.024	2.292TyrLeu: 2.292 ± 0.045	0.454TyrMet: 0.454 ± 0.02	0.638TyrAsn: 0.638 ± 0.026	1.098TyrPro: 1.098 ± 0.033	0.756TyrGln: 0.756 ± 0.027	1.686TyrArg: 1.686 ± 0.032	1.2TyrSer: 1.2 ± 0.032	1.129TyrThr: 1.129 ± 0.038	1.721TyrVal: 1.721 ± 0.036	0.342TyrTrp: 0.342 ± 0.015	0.599TyrTyr: 0.599 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4253 proteins (1271227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---