Mycobacterium phage Target

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MC78|A0A345MC78_9CAUD Uncharacterized protein OS=Mycobacterium phage Target OX=2283260 GN=80 PE=4 SV=1
MM1 pKa = 7.48NDD3 pKa = 3.18EE4 pKa = 4.17LRR6 pKa = 11.84AVLTEE11 pKa = 4.1ALAGHH16 pKa = 5.59QPEE19 pKa = 4.6NYY21 pKa = 9.94GFNCSGCDD29 pKa = 3.34WEE31 pKa = 4.49PANPAVTDD39 pKa = 3.68AAEE42 pKa = 4.29FAAHH46 pKa = 6.3QLDD49 pKa = 4.17VLDD52 pKa = 4.24TAPGVAVIQLPEE64 pKa = 3.98PSYY67 pKa = 11.09VDD69 pKa = 3.52EE70 pKa = 5.5PEE72 pKa = 5.86AGMKK76 pKa = 10.39GVGFNGGPQDD86 pKa = 3.23THH88 pKa = 7.35LSEE91 pKa = 5.01RR92 pKa = 11.84GVYY95 pKa = 9.08VHH97 pKa = 7.59DD98 pKa = 4.28GLVYY102 pKa = 10.32DD103 pKa = 4.1QWDD106 pKa = 4.09CVTPADD112 pKa = 3.89ALKK115 pKa = 10.87VAGWWAAAAVVAAGEE130 pKa = 4.21EE131 pKa = 4.24AA132 pKa = 3.69

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MC02|A0A345MC02_9CAUD Terminase small subunit OS=Mycobacterium phage Target OX=2283260 GN=3 PE=4 SV=1
MM1 pKa = 7.35SWDD4 pKa = 3.28SSDD7 pKa = 3.95RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 4.13RR11 pKa = 11.84LPPDD15 pKa = 3.09WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.4RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.91ADD37 pKa = 3.88ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.08VRR50 pKa = 11.84YY51 pKa = 9.53RR52 pKa = 11.84DD53 pKa = 3.64EE54 pKa = 4.46EE55 pKa = 4.54SPLQASCRR63 pKa = 11.84PCHH66 pKa = 5.76ARR68 pKa = 11.84KK69 pKa = 9.57SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.87PGRR95 pKa = 11.84RR96 pKa = 11.84SNN98 pKa = 3.52

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

15386

13

870

171.0

18.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.035 ± 0.41

0.767 ± 0.115

6.564 ± 0.224

6.662 ± 0.305

3.269 ± 0.168

8.391 ± 0.377

1.917 ± 0.173

4.907 ± 0.22

4.251 ± 0.274

8.495 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.133

3.139 ± 0.17

5.563 ± 0.281

3.25 ± 0.176

6.103 ± 0.362

5.96 ± 0.257

6.33 ± 0.316

7.214 ± 0.248

2.119 ± 0.151

2.925 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski