Amino acid dipepetide frequency for Mycobacterium phage Target

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.975AlaAla: 13.975 ± 1.485
0.91AlaCys: 0.91 ± 0.22
6.695AlaAsp: 6.695 ± 0.653
6.045AlaGlu: 6.045 ± 0.798
3.445AlaPhe: 3.445 ± 0.565
7.995AlaGly: 7.995 ± 0.716
1.43AlaHis: 1.43 ± 0.347
4.355AlaIle: 4.355 ± 0.549
4.81AlaLys: 4.81 ± 0.576
9.23AlaLeu: 9.23 ± 0.936
2.34AlaMet: 2.34 ± 0.402
2.47AlaAsn: 2.47 ± 0.401
4.81AlaPro: 4.81 ± 0.609
2.275AlaGln: 2.275 ± 0.424
6.37AlaArg: 6.37 ± 0.613
5.005AlaSer: 5.005 ± 0.518
5.655AlaThr: 5.655 ± 0.667
8.255AlaVal: 8.255 ± 0.887
1.69AlaTrp: 1.69 ± 0.311
2.6AlaTyr: 2.6 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.455CysAla: 0.455 ± 0.168
0.065CysCys: 0.065 ± 0.074
0.715CysAsp: 0.715 ± 0.216
0.78CysGlu: 0.78 ± 0.262
0.13CysPhe: 0.13 ± 0.103
0.13CysGly: 0.13 ± 0.106
0.26CysHis: 0.26 ± 0.138
0.325CysIle: 0.325 ± 0.163
0.325CysLys: 0.325 ± 0.158
0.455CysLeu: 0.455 ± 0.223
0.195CysMet: 0.195 ± 0.139
0.325CysAsn: 0.325 ± 0.149
0.325CysPro: 0.325 ± 0.151
0.065CysGln: 0.065 ± 0.061
0.78CysArg: 0.78 ± 0.267
0.715CysSer: 0.715 ± 0.21
0.585CysThr: 0.585 ± 0.21
0.455CysVal: 0.455 ± 0.17
0.325CysTrp: 0.325 ± 0.171
0.26CysTyr: 0.26 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
7.085AspAla: 7.085 ± 0.827
0.715AspCys: 0.715 ± 0.269
4.355AspAsp: 4.355 ± 0.573
3.835AspGlu: 3.835 ± 0.481
2.665AspPhe: 2.665 ± 0.358
5.85AspGly: 5.85 ± 0.686
1.04AspHis: 1.04 ± 0.258
2.535AspIle: 2.535 ± 0.518
2.535AspLys: 2.535 ± 0.454
5.785AspLeu: 5.785 ± 0.65
1.43AspMet: 1.43 ± 0.264
2.015AspAsn: 2.015 ± 0.359
4.81AspPro: 4.81 ± 0.619
1.82AspGln: 1.82 ± 0.3
3.25AspArg: 3.25 ± 0.433
3.185AspSer: 3.185 ± 0.45
3.64AspThr: 3.64 ± 0.438
4.615AspVal: 4.615 ± 0.667
2.08AspTrp: 2.08 ± 0.339
2.405AspTyr: 2.405 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
5.785GluAla: 5.785 ± 0.743
0.26GluCys: 0.26 ± 0.166
5.135GluAsp: 5.135 ± 0.533
5.265GluGlu: 5.265 ± 0.698
2.08GluPhe: 2.08 ± 0.407
4.355GluGly: 4.355 ± 0.565
1.495GluHis: 1.495 ± 0.381
3.12GluIle: 3.12 ± 0.44
2.405GluLys: 2.405 ± 0.382
7.345GluLeu: 7.345 ± 0.708
1.56GluMet: 1.56 ± 0.303
1.56GluAsn: 1.56 ± 0.277
2.86GluPro: 2.86 ± 0.514
2.405GluGln: 2.405 ± 0.315
4.095GluArg: 4.095 ± 0.688
3.51GluSer: 3.51 ± 0.444
3.51GluThr: 3.51 ± 0.458
5.72GluVal: 5.72 ± 0.694
1.43GluTrp: 1.43 ± 0.312
2.73GluTyr: 2.73 ± 0.467
0.0GluXaa: 0.0 ± 0.0
Phe
2.535PheAla: 2.535 ± 0.453
0.13PheCys: 0.13 ± 0.102
2.795PheAsp: 2.795 ± 0.368
2.145PheGlu: 2.145 ± 0.343
0.715PhePhe: 0.715 ± 0.238
3.64PheGly: 3.64 ± 0.459
0.845PheHis: 0.845 ± 0.303
1.3PheIle: 1.3 ± 0.334
1.365PheLys: 1.365 ± 0.286
2.21PheLeu: 2.21 ± 0.474
0.78PheMet: 0.78 ± 0.237
1.365PheAsn: 1.365 ± 0.337
1.69PhePro: 1.69 ± 0.317
1.17PheGln: 1.17 ± 0.263
2.015PheArg: 2.015 ± 0.38
1.82PheSer: 1.82 ± 0.336
2.21PheThr: 2.21 ± 0.407
2.275PheVal: 2.275 ± 0.411
0.845PheTrp: 0.845 ± 0.279
0.845PheTyr: 0.845 ± 0.218
0.0PheXaa: 0.0 ± 0.0
Gly
7.02GlyAla: 7.02 ± 0.957
0.585GlyCys: 0.585 ± 0.223
5.46GlyAsp: 5.46 ± 0.497
4.875GlyGlu: 4.875 ± 0.638
3.055GlyPhe: 3.055 ± 0.565
8.255GlyGly: 8.255 ± 1.222
1.82GlyHis: 1.82 ± 0.375
4.875GlyIle: 4.875 ± 0.791
3.965GlyLys: 3.965 ± 0.552
7.475GlyLeu: 7.475 ± 0.774
1.95GlyMet: 1.95 ± 0.389
3.315GlyAsn: 3.315 ± 0.425
3.51GlyPro: 3.51 ± 0.551
2.535GlyGln: 2.535 ± 0.372
3.965GlyArg: 3.965 ± 0.603
5.59GlySer: 5.59 ± 0.635
5.2GlyThr: 5.2 ± 0.759
5.265GlyVal: 5.265 ± 0.643
2.47GlyTrp: 2.47 ± 0.399
2.73GlyTyr: 2.73 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.69HisAla: 1.69 ± 0.341
0.13HisCys: 0.13 ± 0.097
1.04HisAsp: 1.04 ± 0.248
1.56HisGlu: 1.56 ± 0.406
0.585HisPhe: 0.585 ± 0.177
1.56HisGly: 1.56 ± 0.371
0.65HisHis: 0.65 ± 0.196
0.975HisIle: 0.975 ± 0.268
0.78HisLys: 0.78 ± 0.312
1.235HisLeu: 1.235 ± 0.347
0.195HisMet: 0.195 ± 0.163
0.325HisAsn: 0.325 ± 0.138
1.17HisPro: 1.17 ± 0.266
0.91HisGln: 0.91 ± 0.32
1.625HisArg: 1.625 ± 0.383
0.975HisSer: 0.975 ± 0.236
0.975HisThr: 0.975 ± 0.231
1.755HisVal: 1.755 ± 0.306
0.39HisTrp: 0.39 ± 0.147
0.65HisTyr: 0.65 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.915IleAla: 5.915 ± 0.756
0.39IleCys: 0.39 ± 0.145
3.575IleAsp: 3.575 ± 0.42
3.64IleGlu: 3.64 ± 0.482
0.975IlePhe: 0.975 ± 0.257
3.965IleGly: 3.965 ± 0.506
0.715IleHis: 0.715 ± 0.175
1.69IleIle: 1.69 ± 0.365
1.82IleLys: 1.82 ± 0.346
3.64IleLeu: 3.64 ± 0.474
0.845IleMet: 0.845 ± 0.237
1.69IleAsn: 1.69 ± 0.29
3.25IlePro: 3.25 ± 0.434
1.43IleGln: 1.43 ± 0.367
2.925IleArg: 2.925 ± 0.438
3.185IleSer: 3.185 ± 0.452
3.575IleThr: 3.575 ± 0.454
3.25IleVal: 3.25 ± 0.612
0.845IleTrp: 0.845 ± 0.2
1.755IleTyr: 1.755 ± 0.287
0.0IleXaa: 0.0 ± 0.0
Lys
3.575LysAla: 3.575 ± 0.523
0.26LysCys: 0.26 ± 0.134
2.405LysAsp: 2.405 ± 0.477
2.275LysGlu: 2.275 ± 0.406
1.495LysPhe: 1.495 ± 0.321
2.47LysGly: 2.47 ± 0.384
0.91LysHis: 0.91 ± 0.284
2.73LysIle: 2.73 ± 0.506
2.145LysLys: 2.145 ± 0.377
3.445LysLeu: 3.445 ± 0.535
0.91LysMet: 0.91 ± 0.211
1.495LysAsn: 1.495 ± 0.326
3.055LysPro: 3.055 ± 0.427
1.69LysGln: 1.69 ± 0.427
3.38LysArg: 3.38 ± 0.587
2.795LysSer: 2.795 ± 0.379
2.21LysThr: 2.21 ± 0.379
3.38LysVal: 3.38 ± 0.555
0.975LysTrp: 0.975 ± 0.27
0.91LysTyr: 0.91 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
8.84LeuAla: 8.84 ± 0.842
0.325LeuCys: 0.325 ± 0.145
6.565LeuAsp: 6.565 ± 0.691
5.33LeuGlu: 5.33 ± 0.614
2.275LeuPhe: 2.275 ± 0.389
7.15LeuGly: 7.15 ± 0.722
1.56LeuHis: 1.56 ± 0.324
4.485LeuIle: 4.485 ± 0.558
3.9LeuLys: 3.9 ± 0.572
6.045LeuLeu: 6.045 ± 0.645
1.755LeuMet: 1.755 ± 0.397
2.6LeuAsn: 2.6 ± 0.353
5.395LeuPro: 5.395 ± 0.621
2.535LeuGln: 2.535 ± 0.567
6.435LeuArg: 6.435 ± 0.651
5.915LeuSer: 5.915 ± 0.664
5.85LeuThr: 5.85 ± 0.557
4.745LeuVal: 4.745 ± 0.58
1.17LeuTrp: 1.17 ± 0.368
2.08LeuTyr: 2.08 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.6MetAla: 2.6 ± 0.455
0.065MetCys: 0.065 ± 0.076
1.04MetAsp: 1.04 ± 0.267
1.69MetGlu: 1.69 ± 0.319
0.65MetPhe: 0.65 ± 0.158
1.43MetGly: 1.43 ± 0.286
0.325MetHis: 0.325 ± 0.15
0.325MetIle: 0.325 ± 0.138
1.365MetLys: 1.365 ± 0.319
1.235MetLeu: 1.235 ± 0.365
0.13MetMet: 0.13 ± 0.096
0.78MetAsn: 0.78 ± 0.181
1.17MetPro: 1.17 ± 0.244
0.65MetGln: 0.65 ± 0.202
1.43MetArg: 1.43 ± 0.283
2.34MetSer: 2.34 ± 0.446
2.34MetThr: 2.34 ± 0.378
1.17MetVal: 1.17 ± 0.258
0.325MetTrp: 0.325 ± 0.148
0.325MetTyr: 0.325 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.705AsnAla: 3.705 ± 0.645
0.26AsnCys: 0.26 ± 0.153
1.95AsnAsp: 1.95 ± 0.329
2.34AsnGlu: 2.34 ± 0.38
1.17AsnPhe: 1.17 ± 0.322
3.185AsnGly: 3.185 ± 0.526
0.455AsnHis: 0.455 ± 0.198
1.69AsnIle: 1.69 ± 0.294
0.715AsnLys: 0.715 ± 0.212
2.145AsnLeu: 2.145 ± 0.394
0.845AsnMet: 0.845 ± 0.18
0.91AsnAsn: 0.91 ± 0.264
2.795AsnPro: 2.795 ± 0.375
0.845AsnGln: 0.845 ± 0.208
1.235AsnArg: 1.235 ± 0.353
1.495AsnSer: 1.495 ± 0.389
1.365AsnThr: 1.365 ± 0.319
2.34AsnVal: 2.34 ± 0.39
0.715AsnTrp: 0.715 ± 0.202
1.235AsnTyr: 1.235 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
4.94ProAla: 4.94 ± 0.58
0.65ProCys: 0.65 ± 0.244
4.16ProAsp: 4.16 ± 0.511
5.07ProGlu: 5.07 ± 0.621
2.015ProPhe: 2.015 ± 0.358
4.81ProGly: 4.81 ± 0.58
1.04ProHis: 1.04 ± 0.291
2.21ProIle: 2.21 ± 0.398
2.08ProLys: 2.08 ± 0.348
4.095ProLeu: 4.095 ± 0.503
0.78ProMet: 0.78 ± 0.22
1.69ProAsn: 1.69 ± 0.325
3.055ProPro: 3.055 ± 0.408
1.625ProGln: 1.625 ± 0.327
2.925ProArg: 2.925 ± 0.528
3.835ProSer: 3.835 ± 0.48
4.095ProThr: 4.095 ± 0.551
4.29ProVal: 4.29 ± 0.469
0.585ProTrp: 0.585 ± 0.295
1.69ProTyr: 1.69 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
2.665GlnAla: 2.665 ± 0.405
0.26GlnCys: 0.26 ± 0.119
1.495GlnAsp: 1.495 ± 0.338
1.43GlnGlu: 1.43 ± 0.297
1.17GlnPhe: 1.17 ± 0.257
2.535GlnGly: 2.535 ± 0.373
0.455GlnHis: 0.455 ± 0.156
3.12GlnIle: 3.12 ± 0.514
1.495GlnLys: 1.495 ± 0.317
3.705GlnLeu: 3.705 ± 0.475
0.845GlnMet: 0.845 ± 0.259
0.715GlnAsn: 0.715 ± 0.194
1.69GlnPro: 1.69 ± 0.37
1.69GlnGln: 1.69 ± 0.383
1.95GlnArg: 1.95 ± 0.345
1.755GlnSer: 1.755 ± 0.384
1.885GlnThr: 1.885 ± 0.316
2.21GlnVal: 2.21 ± 0.345
0.845GlnTrp: 0.845 ± 0.237
0.585GlnTyr: 0.585 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
5.2ArgAla: 5.2 ± 0.66
0.845ArgCys: 0.845 ± 0.274
3.315ArgAsp: 3.315 ± 0.433
4.03ArgGlu: 4.03 ± 0.608
1.95ArgPhe: 1.95 ± 0.396
4.745ArgGly: 4.745 ± 0.71
1.105ArgHis: 1.105 ± 0.269
2.795ArgIle: 2.795 ± 0.374
3.185ArgLys: 3.185 ± 0.496
5.2ArgLeu: 5.2 ± 0.626
1.95ArgMet: 1.95 ± 0.374
2.015ArgAsn: 2.015 ± 0.374
2.99ArgPro: 2.99 ± 0.443
2.145ArgGln: 2.145 ± 0.365
4.615ArgArg: 4.615 ± 0.641
3.705ArgSer: 3.705 ± 0.575
3.315ArgThr: 3.315 ± 0.602
4.615ArgVal: 4.615 ± 0.549
1.56ArgTrp: 1.56 ± 0.335
1.69ArgTyr: 1.69 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
6.89SerAla: 6.89 ± 0.778
0.455SerCys: 0.455 ± 0.18
3.25SerAsp: 3.25 ± 0.485
3.965SerGlu: 3.965 ± 0.461
1.885SerPhe: 1.885 ± 0.343
6.305SerGly: 6.305 ± 0.568
1.365SerHis: 1.365 ± 0.334
2.99SerIle: 2.99 ± 0.425
2.6SerLys: 2.6 ± 0.514
5.135SerLeu: 5.135 ± 0.525
1.365SerMet: 1.365 ± 0.286
2.08SerAsn: 2.08 ± 0.39
2.665SerPro: 2.665 ± 0.504
2.015SerGln: 2.015 ± 0.337
2.99SerArg: 2.99 ± 0.401
3.38SerSer: 3.38 ± 0.643
3.445SerThr: 3.445 ± 0.517
4.095SerVal: 4.095 ± 0.618
1.365SerTrp: 1.365 ± 0.4
1.365SerTyr: 1.365 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
6.11ThrAla: 6.11 ± 0.668
0.39ThrCys: 0.39 ± 0.19
3.965ThrAsp: 3.965 ± 0.575
3.9ThrGlu: 3.9 ± 0.577
2.34ThrPhe: 2.34 ± 0.416
6.37ThrGly: 6.37 ± 0.899
1.105ThrHis: 1.105 ± 0.272
2.73ThrIle: 2.73 ± 0.513
2.535ThrLys: 2.535 ± 0.498
5.915ThrLeu: 5.915 ± 0.604
1.235ThrMet: 1.235 ± 0.249
1.56ThrAsn: 1.56 ± 0.32
3.835ThrPro: 3.835 ± 0.447
1.82ThrGln: 1.82 ± 0.356
2.73ThrArg: 2.73 ± 0.463
3.77ThrSer: 3.77 ± 0.668
4.225ThrThr: 4.225 ± 0.638
5.2ThrVal: 5.2 ± 0.709
1.3ThrTrp: 1.3 ± 0.272
2.275ThrTyr: 2.275 ± 0.371
0.0ThrXaa: 0.0 ± 0.0
Val
7.67ValAla: 7.67 ± 0.897
0.455ValCys: 0.455 ± 0.163
5.07ValAsp: 5.07 ± 0.643
5.005ValGlu: 5.005 ± 0.639
2.34ValPhe: 2.34 ± 0.458
4.81ValGly: 4.81 ± 0.696
1.43ValHis: 1.43 ± 0.297
3.965ValIle: 3.965 ± 0.543
2.99ValLys: 2.99 ± 0.439
5.915ValLeu: 5.915 ± 0.659
1.235ValMet: 1.235 ± 0.299
2.73ValAsn: 2.73 ± 0.35
3.9ValPro: 3.9 ± 0.436
2.6ValGln: 2.6 ± 0.386
4.745ValArg: 4.745 ± 0.648
4.225ValSer: 4.225 ± 0.591
5.135ValThr: 5.135 ± 0.682
4.68ValVal: 4.68 ± 0.705
1.17ValTrp: 1.17 ± 0.339
2.08ValTyr: 2.08 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
1.43TrpAla: 1.43 ± 0.349
0.26TrpCys: 0.26 ± 0.128
1.495TrpAsp: 1.495 ± 0.311
1.105TrpGlu: 1.105 ± 0.278
0.975TrpPhe: 0.975 ± 0.24
1.69TrpGly: 1.69 ± 0.334
0.65TrpHis: 0.65 ± 0.212
1.235TrpIle: 1.235 ± 0.231
0.325TrpLys: 0.325 ± 0.224
2.08TrpLeu: 2.08 ± 0.427
0.325TrpMet: 0.325 ± 0.19
0.585TrpAsn: 0.585 ± 0.203
1.105TrpPro: 1.105 ± 0.285
0.975TrpGln: 0.975 ± 0.227
1.365TrpArg: 1.365 ± 0.312
1.04TrpSer: 1.04 ± 0.282
1.885TrpThr: 1.885 ± 0.411
1.755TrpVal: 1.755 ± 0.352
0.715TrpTrp: 0.715 ± 0.26
0.195TrpTyr: 0.195 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.275TyrAla: 2.275 ± 0.417
0.26TyrCys: 0.26 ± 0.132
1.17TyrAsp: 1.17 ± 0.331
2.34TyrGlu: 2.34 ± 0.335
0.78TyrPhe: 0.78 ± 0.221
2.665TyrGly: 2.665 ± 0.412
0.52TyrHis: 0.52 ± 0.157
1.625TyrIle: 1.625 ± 0.334
1.17TyrLys: 1.17 ± 0.236
2.665TyrLeu: 2.665 ± 0.45
0.65TyrMet: 0.65 ± 0.199
1.17TyrAsn: 1.17 ± 0.302
1.56TyrPro: 1.56 ± 0.357
1.3TyrGln: 1.3 ± 0.262
2.21TyrArg: 2.21 ± 0.377
1.365TyrSer: 1.365 ± 0.371
2.21TyrThr: 2.21 ± 0.395
2.08TyrVal: 2.08 ± 0.351
0.39TyrTrp: 0.39 ± 0.159
0.845TyrTyr: 0.845 ± 0.225
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (15386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski